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Objective: To understand the current prevalence of clonorchiasis in Jiangxi Province., Methods: A survey was performed according to the scheme of the 3rd Principal Human Parasites of Jiangxi Province. Based on the ecological regions, a stratified cluster sampling method was applied by the economic and geographic situation. In rural areas, the investigation of C. sinensis was carried out together with the soil-transmitted helminths investigation, and in the urban areas, the random cluster sampling method was applied for the C. sinensis investigation. There were 92 survey sites from 32 counties. The eggs of C. sinensis in stool were examined by Kato-Katz technique, and health knowledge was also investigated by questionnaires in some people at the same time., Results: A total of 23 606 sample residents were investigated, and 138 were found infected with C. sinensis , with the infection rate of 0.58%. Light infection was found in most of them. Totally 124 C. sinensis infected persons focused in Xinfeng County, and only a few of infected people scattered in the other counties. In Xinfeng County, 851 residents were investigated. Among them, the infected people were found in all the age groups except the 0- year age group. The highest infection rate appeared in the 70- years group (24.00%). The male infection rate was 20.29%, which was higher than that of the female (6.25%), showing a statistically significant difference ( P ＜0.01). The infection rate was highest in the population who received high school or technical secondary school education (31.48%). For the occupation distribution, the infection rate was highest in public officers (39.39%). The questionnaire survey showed that the infection rate in the populations in Xinfeng County who had the history of eating raw fish or raw shrimp was 33.15%., Conclusions: The distribution of C. sinensis infection presents a regional aggregation in Xinfeng County, but in other areas, the distribution is sporadic. It is necessary to continue to carry out the parasitic disease screening, and in Xinfeng County, it is necessary to strengthen the comprehensive prevention and control intervention.