1. Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020.
- Author
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Konrad R, Eberle U, Dangel A, Treis B, Berger A, Bengs K, Fingerle V, Liebl B, Ackermann N, and Sing A
- Subjects
- COVID-19, COVID-19 Testing, COVID-19 Vaccines, Germany, Humans, Oligonucleotide Array Sequence Analysis, SARS-CoV-2, Sensitivity and Specificity, Time Factors, Viral Envelope Proteins analysis, Viral Envelope Proteins genetics, Workflow, Betacoronavirus genetics, Betacoronavirus isolation & purification, Clinical Laboratory Techniques, Coronavirus Infections diagnosis, Coronavirus Infections genetics, Pneumonia, Viral diagnosis, Pneumonia, Viral genetics, Real-Time Polymerase Chain Reaction methods
- Abstract
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
- Published
- 2020
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