1. Capturing the interactome of newly transcribed RNA.
- Author
-
Bao X, Guo X, Yin M, Tariq M, Lai Y, Kanwal S, Zhou J, Li N, Lv Y, Pulido-Quetglas C, Wang X, Ji L, Khan MJ, Zhu X, Luo Z, Shao C, Lim DH, Liu X, Li N, Wang W, He M, Liu YL, Ward C, Wang T, Zhang G, Wang D, Yang J, Chen Y, Zhang C, Jauch R, Yang YG, Wang Y, Qin B, Anko ML, Hutchins AP, Sun H, Wang H, Fu XD, Zhang B, and Esteban MA
- Subjects
- Animals, Embryonic Stem Cells cytology, Embryonic Stem Cells metabolism, HeLa Cells, High-Throughput Nucleotide Sequencing methods, Humans, Mass Spectrometry methods, Mice, Protein Interaction Maps, RNA genetics, RNA-Binding Proteins genetics, Uridine analogs & derivatives, Uridine chemistry, Click Chemistry methods, Proteome metabolism, RNA metabolism, RNA-Binding Proteins metabolism
- Abstract
We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.
- Published
- 2018
- Full Text
- View/download PDF