1. Detection of substantial porcine group B rotavirus genetic diversity in the United States, resulting in a modified classification proposal for G genotypes
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Marthaler, Douglas, Rossow, Kurt, Gramer, Marie, Collins, James, Goyal, Sagar, Tsunemitsu, Hiroshi, Kuga, Kazufumi, Suzuki, Tohru, Ciarlet, Max, and Matthijnssens, Jelle
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ROTAVIRUSES , *REVERSE transcriptase polymerase chain reaction , *HISTOPATHOLOGY , *NUCLEOTIDE analysis , *VIRAL genetics , *VIRUS diseases in swine - Abstract
Abstract: Rotavirus (RV) is an important cause of gastrointestinal disease in animals and humans. In this study, we developed an RT-PCR to detect RV group B (RVB) and characterized the VP7 (G) gene segment detected in porcine samples. One hundred seventy three samples were tested for RV group A (RVA), RVB, and C (RVC) by RT-PCR and examined for RV-like lesion using histopathology. A majority (86.4%) of the samples had mixed RV infections and co-infections of RVA/RVB/RVC were detected at a higher rate (24.3%) than previously reported. RVB was identified in 46.8% of the 173 samples. An adapted VP7 classification was developed using previously published (n=57) and newly sequenced (n=68) RVB strains, resulting in 20 G genotypes based on an 80% nucleotide identity cutoff value. Our results revealed a broad genetic diversity of porcine RVB strains, suggesting RVB has been the cause of common/pre-existing, yet undiagnosed, disease in pigs. [Copyright &y& Elsevier]
- Published
- 2012
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