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Characterizing the genetic diversity present in a working set of plant germplasm can contribute to its effective management and genetic improvement. The cut flower chrysanthemum ( Chrysanthemum morifolium Ramat.) is an economically important ornamental species. With the repeated germplasm exchange and intensive breeding activities, it remains a major task in genetic research. The purpose of the present study was to characterize the genetic diversity and the population structure of a worldwide collection of 159 varieties, and to apply an association mapping approach to identify DNA-based markers linked to five plant architecture traits and six inflorescence traits. The genotyping demonstrated that there was no lack of genetic diversity in the collection and that pair-wise kinship values were relatively low. The clustering based on a Bayesian model of population structure did not reflect known variation in either provenance or inflorescence type. A principal coordinate analysis was, however, able to discriminate most of the varieties according to both of these criteria. About 1 in 100 marker pairs exhibited a degree of linkage disequilibrium. The association analysis identified a number of markers putatively linked to one or more of the traits. Some of these associations were robust over two seasons. The findings provide an in-depth understanding of genetic diversity and population structure present in cut flower chrysanthemum varieties, and an insight into the genetic control of plant architecture and inflorescence-related traits. [ABSTRACT FROM AUTHOR]

Understanding the genetic architecture is a prerequisite for crop improvement. The current research aimed to characterize the extent of genetic variation of drought tolerance harbored in a global collection of 159 chrysanthemum cultivars over 2 years. An average subordinate function value (ASFV), integrating the wilting index, the fresh weight retention rate, and the survival rate after re-watering under two drought-stressed trials, was used to quantify the level of drought tolerance. The performance of ASFV was generally correlated between the 2 years; and a high magnitude (0.95) of broad-sense heritability, coupled with the moderate genetic advance, was estimated for the ASFV. By applying MLM model with both population structure and kinship matrix as covariates association mapping identified 16 markers linked to drought tolerance, with the proportion of the phenotypic variation explained by an individual marker ranging from 4.4 to 7.6%. Of the eight markers predictive across the 2 years, four (E11M24-9, E3M2-8, E1M5-5, and EST-SSR34-3) were identified as favorable alleles for drought tolerance. Several cultivars that carry at least three of the four favorable alleles were identified as potential donor parents for future improvement of the drought tolerance. The findings provide an insight into the genetic basis of the drought tolerance in chrysanthemum and will, therefore, aid in developing new cultivars with enhanced tolerance against drought stress. [ABSTRACT FROM AUTHOR]

Highlights • CmTCP20 belonged to PCF in Class I of TCP transcription factor family. CmTCP20 localized on nucleus and presented no transcriptional activation. • OX-CmTCP20 in chrysanthemum led to the longer petals with longer cells, and the CmBPE2 expression level was down-regulated in OX-CmTCP20 lines. • Ectopic expression in S. pombe yeast cells showed that CmTCP20 could repress cell division and promote cell elongation. • Yeast two-hybrid and BiFC experiments results indicated that CmTCP20 interacted with CmJAZ1-like. Abstract Chrysanthemum morifolium is one of the most popular ornamental species worldwide, with high ornamental and economic value. Petal size is an important factor that influences the ornamental value. CmTCP20 is a member of TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORs (TCPs) gene family, which is closely associated with the growth and development of plants. Our previous study found that the expression of CmTCP20 was obviously down-regulated during chrysanthemum petal elongation, but its function in petal elongation has not yet been revealed. We show here that the overexpression CmTCP20 in Arabidopsis and chrysanthemum leads to similar phenotypes, including larger flower buds (or inflorescences) and longer petals. Interestingly, ectopic expression in Schizosaccharomyces pombe yeast cells showed that CmTCP20 could repress cell division and promote cell elongation. Moreover, the yeast two-hybrid, BiFC and pull-down experimental results indicated that CmTCP20 may regulate petal size via interacting with CmJAZ1-like and inducing down-regulation of CmBPE2 gene expression. This study preliminarily clarifies the function of CmTCP20 on chrysanthemum petal elongation, providing the basic theory for improving the ornamental characteristic of chrysanthemum. [ABSTRACT FROM AUTHOR]