1. Chromosomal status of uni-pronuclear human zygotes following in-vitro fertilization and intracytoplasmic sperm injection.
- Author
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Sultan KM, Munné S, Palermo GD, Alikani M, and Cohen J
- Subjects
- Adult, Cell Nucleus ultrastructure, Chromosome Aberrations, Cytoplasm, Female, Humans, In Situ Hybridization, Fluorescence, Male, Microinjections, Parthenogenesis, Ploidies, Spermatozoa, Y Chromosome, Chromosomes, Human, Fertilization in Vitro, Reproductive Techniques, Zygote ultrastructure
- Abstract
Uni-pronuclear embryos (n = 42) were analysed by fluorescence in-situ hybridization (FISH) with two to four chromosome pair-specific probes. Half of these embryos resulted from conventional insemination and half from intracytoplasmic sperm injection (ICSI). The majority of uni-pronuclear embryos from conventional insemination were normally diploid (61.9%) whereas only 9.5% of uni-pronuclear ICSI embryos (P < 0.001) were diploid. In addition, a significantly higher number of uni-pronuclear embryos from conventional insemination had a Y chromosome (10/21, 47.6%) when compared with ICSI embryos (2/21, 9.5%) (P = 0.015). It is concluded that the majority of uni-pronuclear embryos following regular in-vitro fertilization are fertilized, whereas those from ICSI are parthenogenetically activated. The latter embryos should not be considered for embryo replacement. more...
- Published
- 1995
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