1. Rb1 gene expression in B-cell lymphocytic leukaemia cases with deletion in the 13q14 region.
- Author
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Antosz H, Kitlińska J, Kwiatkowska-Drabik B, Koczkodaj D, Wach M, Antosz M, Rudzki S, and Wojcierowski J
- Subjects
- Cells, Cultured, Chromosome Aberrations genetics, Chromosome Banding, Female, Humans, Immunophenotyping, Karyotyping, Lymphocytes, Male, Polymerase Chain Reaction, RNA, Messenger blood, RNA, Neoplasm blood, Chromosome Deletion, Chromosomes, Human, Pair 13 genetics, Gene Expression Regulation, Leukemic genetics, Genes, Retinoblastoma genetics, Leukemia, Lymphocytic, Chronic, B-Cell genetics
- Abstract
Recent studies have been carried out to delineate further the role of the Rb1 gene in B-cell chronic lymphocytic leukaemia (B-CLL). The suggested role of the Rb1 gene in this disease was based on cytogenetic data. CLL patients (40 in toto) were examined using cytogenetic and molecular biological methods. R-banding analysis of metaphase chromosomes revealed aberrations in only seven cases containing either the Rb1 gene or a chromosome 13 monosomy. No evident differences were found by RT-PCR analysis of Rb1 gene expression. The amounts of the RT-PCR products obtained appeared to be approximately equal in all cases, and was independent of the clinical stage, immunophenotypes and LPS or TPA stimulation.
- Published
- 1997