1. Melanin: Quantification and protection against oxidative stress in chromoblastomycosis agents.
- Author
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Heidrich D, Corbellini VA, Mendes SDC, Fernandes EK, Lazzarotto L, Ribeiro AC, Zanette RA, and Scroferneker ML
- Subjects
- Antifungal Agents pharmacology, Ascomycota drug effects, Ascomycota isolation & purification, Humans, Hydrogen Peroxide pharmacology, Melanins biosynthesis, Microbial Viability drug effects, Phialophora chemistry, Phialophora drug effects, Phialophora isolation & purification, Phialophora physiology, Species Specificity, Spores, Fungal physiology, Thiazoles pharmacology, Ascomycota chemistry, Ascomycota physiology, Chromoblastomycosis microbiology, Melanins analysis, Oxidative Stress drug effects
- Abstract
Chromoblastomycosis (CBM) is a chronic cutaneous and subcutaneous infection caused by melanized fungal species. We quantified the extractable melanin of 77 strains of CBM agents distributed within five genera. Moreover, resistance to oxidative stress was evaluated in strains exposed or not to the melanin inhibitor tricyclazole. The median percentage of melanin mass extracted from dry fungal mass varied from 0.69 (Rhinocladiella similis) to 3.81 (Phialophora americana). Inhibition of melanin synthesis decreased survival rates to hydrogen peroxide. Together, these data highlight the importance of melanin in CBM agents.
- Published
- 2019
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