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In addition to blood glucose testing, monitoring of blood glycated proteins got considerable attention on the glycemic control of diabetes. Glycated albumin has been reported as a good complementary to or better indicator than glycated hemoglobin for the evaluation of short-term glycemic control. Herein, the development of magnetic particle-based immunoassays for blood glycated proteins is demonstrated. Spherical magnetic particles are fabricated composed of a polystyrene core covered with a thin layer of Fe3O4 and SiO2. The core-shell structure allow us to control a wide size range by using the polystyrene cores of different size, and the silica outer layer can be functionalized easily with silane coupling agents. Aminophenylboronic acid is covalently immobilized onto magnetic particles for the affinity isolation of glycated proteins, while horseradish peroxidase labeled antibodies are used for detection. The assays are optimized and applied successfully to the determination of glycated albumin in standard and human serum samples and show good potential as tools for diabetes monitoring.

We developed a fast and precision immunoassay-based microfluidic assay system that consisted of a microfluidic analytic cartridge and immunoassay reagents for point-of-care testing (POCT). The nano-magnetic beads conjugated antibodies, HRP-labeled analog, and color reagent were all pre-loaded and packaged in a microfluidic analytic cartridge. The whole assay process was completed within 30 min without any additional washing or loading of reagents once the sample was injected in the microfluidic analytic cartridge. The performance of the immunoassay-based microfluidic assay system had been examined by using mouse IgG model. The results indicated that the dynamic range of the measurement could be approached to two orders with low deviation. The present study demonstrated that the performance of our immunoassay-based microfluidic assay system was suitable for POCT for healthcare professionals or patients.