1. Application of Dried Blood Spot Cards combined with liquid chromatography-tandem mass spectrometry to determine eight fat-soluble micronutrients in human blood
- Author
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Maria-José Motilva, Maria-Paz Romero, Lorena Calderón, Alba Macià, Judit Companys, Laura Pla-Pagà, Laura Rubió, Iziar A. Ludwig, Silvia Yuste, Ministerio de Economía y Competitividad (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Universidad de Lleida, and Universidad Rovira i Virgili
- Subjects
Adult ,Male ,Lutein ,Clinical Biochemistry ,Tocopherols ,Hematocrit ,Tandem mass spectrometry ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Liquid chromatography–mass spectrometry ,Limit of Detection ,Tandem Mass Spectrometry ,medicine ,Humans ,Micronutrients ,Vitamin A ,Tandem MS ,Whole blood ,DBS cards ,Detection limit ,Chromatography ,medicine.diagnostic_test ,Retinol ,Chemistry ,010401 analytical chemistry ,food and beverages ,Reproducibility of Results ,Cell Biology ,General Medicine ,Middle Aged ,Carotenoids ,0104 chemical sciences ,Dried blood spot ,Linear Models ,Female ,Dried Blood Spot Testing ,Blood sampling ,Chromatography, Liquid - Abstract
The analysis of the fat-soluble vitamins A and E and lipid micronutrients in blood, such as carotenoids, is an important parameter to monitor the micronutrient status in humans. Although the potential of dried blood spot (DBS) cards, the use of this technique for blood sampling and subsequent analysis of these fat-soluble micronutrients has been poorly or not studied. An analytical method based on DBS cards (FTA® DMPK-A) combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been developed and validated for the determination of carotenoids (lutein, zeaxanthin, β-cryptoxanthin and β-carotene), tocopherols (α-tocopherol, γ-tocopherol and δ-tocopherol) and all-trans-retinol in human blood. Under optimum DBS card extraction conditions, the extraction recoveries of the studied compounds were higher than 72%, the sample matrix effect lower than 17%, and the detection limits at hundred nM concentration levels. The developed method was applied to the analysis of human blood, and the concentration ranges obtained fell within the expected ranges previously reported in healthy adults. Moreover, the influence of hematocrit effect was investigated in a range of 25–55% in order to compare the obtained results to those reported in the literature for the analysis of plasma samples. This method represents an improvement over current techniques reported in the literature due to the use of a non-invasive blood collection method, and moreover, this methodology was for the first time 1) validated for the analysis of all-trans-retinol, tocopherols and carotenoids, and 2) applied for the determination of tocopherols in human blood samples., This study was supported by the Spanish Ministry of Industry, Economy and Competitiveness through the AGL2016-76943-C2-1-R and AGL2016-76943-C2-2-R projects (co-funded by the European Social Fund, European Union); M.J. Motilva thanks to Consejo Superior de Investigaciones Científicas-CSIC for partial funding throught the “Ayudas incorporación a escalas científicas CSIC, 2018” (Reference 201870I129). I.A.L. enjoys a post-doctoral contract (2017PMF-POST2-19) from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement and from the Universitat Rovira i Virgili (URV). S.Y. was supported by a pre-doctoral grant from the University of Lleida.
- Published
- 2020