1. Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system.
- Author
-
Perez RH, Himeno K, Ishibashi N, Masuda Y, Zendo T, Fujita K, Wilaipun P, Leelawatcharamas V, Nakayama J, and Sonomoto K
- Subjects
- Anti-Bacterial Agents analysis, Bacteriocins analysis, Fermentation, Peptides isolation & purification, Temperature, Anti-Bacterial Agents biosynthesis, Bacteriocins biosynthesis, Chromatography, Liquid methods, Enterococcus faecium metabolism, Spectrometry, Mass, Electrospray Ionization methods
- Abstract
Enterococcus faecium NKR-5-3 produces four antimicrobial peptides referred here as enterocins NKR-5-3A, B, C and D. A two-step electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS)-based quantification system was developed to monitor its multiple bacteriocin production profiles, which is essential in understanding the complex production regulation mechanism of strain NKR-5-3. The developed ESI-LC/MS-based quantification system can easily monitor the multiple bacteriocin production of this strain. Using the developed system, the production of enterocin NKR-5-3B was found to be not as variable as those of the other enterocins in different cultivation media. Production of enterocin NKR-5-3B was also found to have a wider optimum incubation temperature (20-30°C) than enterocins NKR-5-3A, C and D (25°C). Furthermore, at least 2 nM of the bacteriocin-like inducing peptide, enterocin NKR-5-3D, regulated the production of NKR-5-3 enterocins except enterocin NKR-5-3B. These findings taken together suggest that enterocin NKR-5-3B has an independent production regulation mechanism from the other NKR-5-3 enterocins. The developed system could effectively pin-point the production profiles of the multiple bacteriocins of E. faecium NKR-5-3 under different fermentation conditions., (Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF