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2 results on '"Pantić, Igor"'

1. The influence of lithium sulphate on Shannon entropy in lymphocyte chromatin

Author

Jeremić Marta, Pantić Igor, and Jakšić Mila

Subjects
lithium, chromatin, lymphocytes, Shannon entropy, Medicine
Abstract

Introduction: Lithium affects numerous signal pathways in cells, which may ultimately lead to either increased or decreased gene expression in cell nuclei. However, effects of lithium on higher level of gene organization in the nuclei, like chromatin, is still poorly understood. Aim: To investigate the effects of lithium on chromatin organization in the nuclei of lymphocytes by investigating changes in Shannon entropy of chromatin in these cells. Material and Methods: Peripheral blood was treated with lithium sulphate until lithium concentrations of 1mmol/l, 2 mmol/l i 3 mmol/l was not reached. The smears were fixed with methanol and stained by Felgen method for DNK visualization. Cells in the smear were treated with hydrochloric acid for 120 min. Schiff reagent was used for staining for 120 min and smear washing was done with 6 ml of 10% of water solution of natrium metabisulfite. After staining, digital micrographs for each of the most representative sample of 30 lymphocytic chromatin structures were made. Shannon entropy was calculated by converting micrograph in the format of textual numerical string. Lymphocyte chromatin was also analyzed by textural method analysis. Results: Treatment with 1 mmol/l of lithium sulphate did not lead to a statistically significant increase in entropy values (p > 0.05). However, in samples where the concentration of lithium was 2 i 3 mmol/l, respectively, there was a statistically significant increase in entropy (p < 0.05). Also, a statistically significant and dose-dependent linear trend of an increase of chromatin entropy was detected in samples (p < 0.05). After lithium sulphate treatment, neither the mean value of angular second moment lymphocyte chromatin in control sample nor the inverse moment of the difference of treated lymphocytes changed (p > 0.05). Conclusion: Lithium sulphate in peripheral blood lymphocytes causes dose-dependent increase in Shannon chromatin entropy, which is not followed with similar changes in a textural chromatin parameters.

Published
2018

2. Fractal and textural analysis of blood lymphocyte chromatin structure in patients with different grades of depression

Author

Dimitrijević, Draga N., Pantić, Igor, Damjanović, Aleksandar, Nešić, Dejan, and Vukomanović Đurđević, Biserka

Subjects
Depression, Entropy, Fraktal, Depresija, Lakunarnost, Lacunarity, Hromatin, Fractal, Entropija, Chromatin
Abstract

Imajući u vidu dokazanu povezanost između promena u strukturnoj kompleksnosti tkiva i pojedinih fizioloških i patoloških procesa, pretpostavili smo da u toku depresije dolazi do smanjenja strukturne kompleksnosti nukleusnog hromatina limfocita, odnosno da sa povećanjem depresivnih simptoma dolazi do smanjenja hromatinske fraktalne dimenzije. Takođe smo pretpostavili da su depresivni simptomi u pozitivnoj korelaciji sa nivoom hromatinske neuređenosti, odnosno da teksturalna entropija hromatina limfocita raste sa povećanjem depresije kod pacijenata. Ciljevi istraživanja su bili: (1) Utvrditi postojanje razlike u fraktalnim i teksturalnim parametrima hromatina limfocita između pacijenata sa depresijom, i ispitanika koji ne zadovoljavaju dijagnostičke kriterijume za depresiju i (2) Utvrditi postojanje i jačinu korelacije između stepena depresije i fraktalnih, odnosno teksturalnih parametara hromatina limfocita Metod. Istraživanje je dizajnirano po tipu studije preseka i obavljeno na uzorku od 100 ispitanika, od kojih 50 pacijenata obolelih od rekurentnog depresivnog poremećaja lečenih u Klinici „Dr Laza Lazarević“ u Beogradu. Kontrolnu grupu je činilo 50 ispitanika koji su pripadali drugim dijagnostičkim kategorijama, a nisu ispunjavali dijagnostičke kriterijume za depresiju. Eksperimentalni protokol je sproveden na sledeći način: uzimanje detaljne istorije bolesti, primena Bekove skale za procenu depresije i Skale 5 depresivnosti, anksioznosti i stresa DASS–21, uzimanje krvi za standardne hormonske (tireoidni hormoni) i biohemijske analize... Introduction. Having in mind already proven connection between changes in tissue complexity and certain physiological and pathological processes, we assumed that during the depression the structural complexity of the nucleic lymphocytes chromate is reduced, that is, with the increase in depressive symptoms, the chromatin fractal dimension decreases. We also assumed that depressive symptoms were in a positive correlation with the level of chromatic disorder, that textural entropy of lymphocyte chromatin was increasing with increasing depression in patients. The objectives of the research were: (1) to determine the existence of a difference in the fractal and textural parameters of lymphocyte chromatin between patients with depression and subjects who did not meet the diagnostic criteria for depression; and (2) determine the existence and strength of the correlation between the degree of depression and fractal or textural parameters of chromatin in lymphocytes. Methods. The study was designed as a cross section study, and performed on a sample of 100 subjects, of which 50 patients with recurrent depressive disorder treated at the Clinic "Dr Laza Lazarević" in Belgrade. The control group consisted of 50 subjects who belonged to other diagnostic categories and did not fulfill the diagnostic criteria for depression. The experimental protocol was carried out as follows: taking a detailed history of the disease, applying the Beck scale to assess depression, and Depression, anxiety 8 and stress scales DASS-21, taking blood for standard hormone (thyroid hormones) and biochemical analysis...

Published
2018

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