1. Degradation of actin nucleators affects cortical polarity of aged mouse oocytes
- Author
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Sun, Shao-Chen, Gao, Wei-Wei, Xu, Yong-Nan, Jin, Yong-Xun, Wang, Qing-Ling, Yin, Xi-Jun, Cui, Xiang-Shun, and Kim, Nam-Hyung
- Subjects
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OVUM , *LABORATORY mice , *CHORIONIC gonadotropins , *CAFFEINE , *HEALTH outcome assessment , *GENE expression , *ACTIN , *REVERSE transcriptase polymerase chain reaction - Abstract
Objective: To investigate the molecular mechanism of mouse oocyte polarity loss during aging. Design: Experimental study. Setting: Academic basic research laboratory. Animal(s): Mice. Intervention(s): Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine. Main Outcome Measure(s): Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription–polymerase chain reaction. Result(s): The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes. Conclusion(s): Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes. [Copyright &y& Elsevier]
- Published
- 2012
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