1. Oxidative stress assessment in sickle cell anemia patients treated with hydroxyurea.
- Author
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Renó, Cristiane O., Barbosa, Amanda Rodrigues, de Carvalho, Sara Santos, Pinheiro, Melina B., Rios, Danyelle Romana, Cortes, Vanessa F., Barbosa, Leandro A., and Santos, Hérica L.
- Subjects
SICKLE cell anemia ,CHOLESTEROL content of food ,OXIDATIVE stress ,GLUTATHIONE reductase ,GLUTATHIONE peroxidase ,ERYTHROCYTE membranes ,ERYTHROCYTE metabolism ,DRUG therapy for sickle cell anemia ,RESEARCH ,FERRANS & Powers Quality of Life Index ,CLINICAL trials ,HYDROXYUREA ,HEMOLYSIS & hemolysins ,RESEARCH methodology ,EVALUATION research ,MEDICAL cooperation ,COMPARATIVE studies ,PHOSPHOLIPIDS ,CHOLESTEROL - Abstract
Hydroxyurea (HU) is used as a therapy in sickle cell anemia (SCA). Many studies have established that HU improves patient quality of life by reducing symptoms. However, the effect of HU on erythrocytes is not well-described. We evaluated several parameters related to oxidative stress and total lipid content of erythrocytes in patients with SCA. The patient cohort consisted of 7 SCA patients treated with HU, 17 untreated SCA patients, and 15 healthy subjects. Erythrocytes from patients with SCA displayed increased oxidative stress relative to the control group, including higher thiobarbituric acid reactive substances (TBARS), Fe3+ content, and osmotic fragility, and decreased total cholesterol. We observed that treatment of SCA patients with HU increased Fe3+ content and activity of glutathione peroxidase, and decreased glutathione reductase activity, glutathione levels, total cholesterol, and phospholipid content comaperaded to patients untreated with HU. Thus, HU alters biochemical characteristics of erythrocytes; future studies will determine whether they are beneficial or not. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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