1. Flavonoids and saponins extracted from black bean (Phaseolus vulgaris L.) seed coats modulate lipid metabolism and biliary cholesterol secretion in C57BL/6 mice.
- Author
-
Chavez-Santoscoy RA, Gutierrez-Uribe JA, Granados O, Torre-Villalvazo I, Serna-Saldivar SO, Torres N, Palacios-González B, and Tovar AR
- Subjects
- Animals, Bile Acids and Salts metabolism, Cells, Cultured, Cholagogues and Choleretics chemistry, Cholagogues and Choleretics isolation & purification, Cholagogues and Choleretics metabolism, Cholesterol metabolism, Flavonoids chemistry, Flavonoids isolation & purification, Flavonoids metabolism, Gene Expression Regulation, Hepatocytes cytology, Hepatocytes enzymology, Hepatocytes metabolism, Lipid Metabolism, Lipotropic Agents chemistry, Lipotropic Agents isolation & purification, Lipotropic Agents metabolism, Liver X Receptors, Male, Mexico, Mice, Mice, Inbred C57BL, Orphan Nuclear Receptors agonists, Orphan Nuclear Receptors genetics, Orphan Nuclear Receptors metabolism, Plant Extracts chemistry, Plant Extracts isolation & purification, Plant Extracts metabolism, Plant Extracts therapeutic use, Random Allocation, Rats, Saponins chemistry, Saponins isolation & purification, Saponins metabolism, Cholagogues and Choleretics therapeutic use, Dietary Supplements, Flavonoids therapeutic use, Lipotropic Agents therapeutic use, Phaseolus chemistry, Saponins therapeutic use, Seeds chemistry
- Abstract
Black bean (Phaseolus vulgaris L.) seed coats are a rich source of natural compounds with potential beneficial effects on human health. Beans exert hypolipidaemic activity; however, this effect has not been attributed to any particular component, and the underlying mechanisms of action and protein targets remain unknown. The aim of the present study was to identify and quantify primary saponins and flavonoids extracted from black bean seed coats, and to study their effects on lipid metabolism in primary rat hepatocytes and C57BL/6 mice. The methanol extract of black bean seed coats, characterised by a HPLC system with a UV-visible detector and an evaporative light-scattering detector and HPLC-time-of-flight/MS, contained quercetin 3-O-glucoside and soyasaponin Af as the primary flavonoid and saponin, respectively. The extract significantly reduced the expression of SREBP1c, FAS and HMGCR, and stimulated the expression of the reverse cholesterol transporters ABCG5/ABCG8 and CYP7A1 in the liver. In addition, there was an increase in the expression of hepatic PPAR-α. Consequently, there was a decrease in hepatic lipid depots and a significant increase in bile acid secretion. Furthermore, the ingestion of this extract modulated the proportion of lipids that was used as a substrate for energy generation. Thus, the results suggest that the extract of black bean seed coats may decrease hepatic lipogenesis and stimulate cholesterol excretion, in part, via bile acid synthesis.
- Published
- 2014
- Full Text
- View/download PDF