Search

This paper investigates the effect of rootstock (Gisela 6, PiKu 1 and SL 64) on the total phenol content, total anthocyanin content, the content of the individual phenols and the antioxidant activity in fruits of two sweet cherry cultivars, 'Kordia' and 'Regina'. The total phenolic content determined by the spectrophotometric method using Folin-Ciocalteu reagent varied from 34.84 to 149.28 to mg GAE/100 g FW depending on the cultivar/rootstock combination. The concentration of total anthocyanins was determined by using the pH-differential method and it ranged from 0.46 to 11.54 mg CGE/100 g FW. Highest level of the total phenolic content and concentration of the total anthocyanins content was detected in the cultivar 'Regina' grafted onto the Gisela 6 rootstock. Neochlorogenic acid, catechin, chlorogenic acid and quercetine-3-O-glucoside were detected using HPLC method. Significant variation of detected individual polyphenols in sweet cherry fruits grafted on different rootstocks was observed. The lowest content of individual polyphenols was measured in 'Regina' cultivar grafted on the SL 64. Ferric reducing antioxidant power assay indicated that all investigated fruits possessed similar antioxidant activity. There was a statistically significant correlation observed between the total phenolic content and antioxidant activity (correlation coefficient 0.972, P-value below 0.01), as well as between the anthocyanins and antioxidant activity (correlation coefficient 0.855, P-value below 0.01). [ABSTRACT FROM AUTHOR]

The present study aimed to investigate the influence of different rootstocks (Gisela 6, Pi-Ku 1, and SL 64) on various parameters, including total phenol content, total anthocyanin content, individual phenol content, and antioxidant activity in the fruits of two sweet cherry cultivars, namely Early Lory and Prime Giant. The determination of total phenolic content was carried out using the Folin-Ciocalteu reagent and spectrophotometric method, resulting in a range of 34.77±1.76 to 88.58±8.83 mg GAE/100 g FW, depending on the specific combination of cultivar and rootstock. The concentration of total anthocyanins, determined through the pH-differential method, varied from 1.08±0.07 to 18.62±0.66 mg CGE/100 g FW. Among the different combinations, the highest levels of total phenolic content and total anthocyanin concentration were found in Early Lory cultivar grafted onto Pi-Ku 1 rootstock. Using HPLC analysis, neochlorogenic acid, catechin, chlorogenic acid, p-coumaric acid and quercetin-3-O-glucoside were detected as individual phenols, exhibiting significant variation among sweet cherry fruits grafted on different rootstocks. The lowest content of the investigated individual polyphenols was observed in Early Lory grafted onto Pi-Ku 1 rootstock. Furthermore, the ferric reducing antioxidant power assay indicated higher antioxidant activity in Early Lory cultivar compared to Prime Giant. A statistically significant correlation was observed between total phenolic content and antioxidant activity (0.978 p<0.01), as well as between anthocyanins and antioxidant activity (0.956 p<0.01). [ABSTRACT FROM AUTHOR]