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Start Over Descriptor "c.trachomatis" Topic chlamydia trachomatis
3 results on '"c.trachomatis"'

1. Cost-benefit analysis of Chlamydia trachomatis screening in pregnant women in a high burden setting in the United States.

Author

Ditkowsky, Jared, Shah, Khushal H., Hammerschlag, Margaret R., Kohlhoff, Stephan, and Smith-Norowitz, Tamar A.

Subjects
*CHLAMYDIA trachomatis, *PREGNANT women, *PREGNANCY complications, *MEDICAL screening, *PUBLIC health, *DIAGNOSIS
Abstract

Background: Chlamydia trachomatis is the most common bacterial sexually transmitted infection (STI) in the United States (U.S.) [1] and remains a major public health problem. We determined the cost- benefit of screening all pregnant women aged 15-24 for Chlamydia trachomatis infection compared with no screening.Methods: We developed a decision analysis model to estimate costs and health-related effects of screening pregnant women for C. trachomatis in a high burden setting (Brooklyn, NY). Outcome data was from literature for pregnant women in the 2015 US population. A virtual cohort of 6,444,686 pregnant women, followed for 1 year was utilized. Using outcomes data from the literature, we predicted the number of C. trachomatis cases, associated morbidity, and related costs. Two comparison arms were developed: pregnant women who received chlamydia screening, and those who did not. Costs and morbidity of a pregnant woman-infant pair with C. trachomatis were calculated and compared.Results: Cost and benefit of screening relied on the prevalence of C. trachomatis; when rates are above 16.9%, screening was proven to offer net cost savings. At a pre-screening era prevalence of 8%, a screening program has an increased expense of $124.65 million ($19.34/individual), with 328 thousand more cases of chlamydia treated, and significant reduction in morbidity. At a current estimate of prevalence, 6.7%, net expenditure for screening is $249.08 million ($38.65/individual), with 204.63 thousand cases of treated chlamydia and reduced morbidity.Conclusions: Considering a high prevalence region, prenatal screening for C. trachomatis resulted in increased expenditure, with a significant reduction in morbidity to woman-infant pairs. Screening programs are appropriate if the cost per individual is deemed acceptable to prevent the morbidity associated with C. trachomatis. [ABSTRACT FROM AUTHOR]

Published
2017
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2. In-cell western assay as a high-throughput approach for Chlamydia trachomatis quantification and susceptibility testing to antimicrobials

Author

Rosa Sessa, Fabiana Diaco, Marisa Di Pietro, Simone Filardo, Patrizio Pasqualetti, and Martina Manera

Subjects
0301 basic medicine, c.trachomatis, Serial dilution, Cervicitis, Chlamydia trachomatis, Pathology and Laboratory Medicine, medicine.disease_cause, Chlamydia Infection, Medical Conditions, Antibiotics, Medicine and Health Sciences, Chlamydia, Staining, Multidisciplinary, Antimicrobials, Drugs, Cell Staining, Antimicrobial, Anti-Bacterial Agents, Bacterial Pathogens, Infectious Diseases, Medical Microbiology, erythromycin, in-cell western, direct immunofluorescence, Engineering and Technology, Medicine, Biological Cultures, Pathogens, Research Article, medicine.drug, Science, Blotting, Western, 030106 microbiology, Sexually Transmitted Diseases, Erythromycin, Microbial Sensitivity Tests, Research and Analysis Methods, Microbiology, Antibiotic Susceptibility Testing, Cell Line, 03 medical and health sciences, Minimum inhibitory concentration, Microbial Control, medicine, Humans, Urethritis, Signal to Noise Ratio, Microbial Pathogens, Direct fluorescent antibody, Pharmacology, Bacteria, business.industry, Organisms, Biology and Life Sciences, Chlamydia Infections, Cell Cultures, medicine.disease, Virology, Bacterial Load, Pharmacologic Analysis, 030104 developmental biology, Specimen Preparation and Treatment, Signal Processing, Feasibility Studies, business
Abstract

Chlamydia trachomatis, the leading cause of bacterial sexually transmitted diseases in developed countries, with around 127 million new cases per year, is mainly responsible for urethritis and cervicitis in women, and urethritis and epididymitis in men. Most C. trachomatis infections remain asymptomatic (>50%) and, hence, untreated, leading to severe reproductive complications in both women and men, like infertility. Therefore, the detection of C. trachomatis as well as the antimicrobial susceptibility testing becomes a priority, and, along the years, several methods have been recommended, like cell culture and direct immunofluorescence (DFA) on cell cultures. Herein, we described the application of In-Cell Western assay (ICW) via Odyssey CLx as a fast, more accessible, and high-throughput platform for the quantification of C. trachomatis and the screening of anti-chlamydial drugs. As a first step, we set up a standard curve by infecting cell monolayers with 2-fold serial dilutions of C. trachomatis Elementary Body (EB) suspension. Then, different unknown C. trachomatis EB suspensions were quantified and the chlamydial susceptibility testing to erythromycin was performed, using the DFA as comparison. Our results showed a very high concordance between these two assays, as evidenced by the enumeration of chlamydial IFUs as well as the determination of erythromycin Minimum Inhibitory Concentration (MIC). In conclusion, the ICW assay may be a promising candidate as an accurate and accessible methodology for C. trachomatis antimicrobial susceptibility testing.

Published
2021
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3. Genital chlamydial infection in STD patients: Its relation to HIV infection

Author

M. Ganapathy, S P Thyagarajan, Joyee Ag, E. V. Reddy, and C. Venkatesan

Subjects
Microbiology (medical), Adult, Male, medicine.medical_specialty, C.trachomatis, HIV Positivity, lcsh:QR1-502, Human immunodeficiency virus (HIV), Sexually Transmitted Diseases, Chlamydia trachomatis, HIV Infections, Std clinic, medicine.disease_cause, lcsh:Microbiology, Risk Factors, Internal medicine, HIV Seropositivity, medicine, Humans, Sex organ, General hospital, Chlamydia, business.industry, HIV, virus diseases, Chlamydia Infections, medicine.disease, Clinical research, Immunology, HIV-1, Female, business, Chlamydial infection
Abstract

In the present report, we have analysed C.trachomatis infection and HIV positivity among patients (n-143) who attended the STD clinic at the Institute of STDs, Government General Hospital, Chennai. HIV positivity rate was significantly high among those with chlamydial infection than in those without chlamydial infection (29.5% (13/44) vs. 11.1% (11/99); p<0.05). The results of the present study suggest the association between C.trachomatis and HIV infections and reinforce the need for routine screening for C.trachomatis as a necessary intervention to reduce the burden of chlamydial diseases and to reduce the risk of HIV and its spread in India.

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