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Clove essential oil is a natural and highly effective bacteriostatic agent, but its poor water solubility limits its wide application. Tn this study，palmitic acid (PA) was used as the hydrophobic group and N-acetyl-L cysteine (NAC) was used as the hydrophilic group to synthesize amphiphilic chitosan derivatives (PA-CSNAC), and further prepared clove essential oil-amphiphilic chitosan drug-loaded nanomicelles (NMs). Fourier infrared spectroscopy and nuclear magnetic resonance hydrogen spectroscopy showed that both PA and NAC were successfully grafted onto chitosan. Scanning electron microscopy results showed that the morphology of NMs showed a uniformly dispersed spherical shape. The research results have shown that clove essential oil-amphiphilic chitosan-loaded nanomicelles had pH-responsive performance at room temperature, and their antibacterial properties against Botrytis Cinerea increased by more than 10% compared with unembedded clove essential oil，where the performance of bacteria was improved significantly. [ABSTRACT FROM AUTHOR]