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2. Detection and Identification of 16SrII Group Phytoplasmas Infecting Stylosanthes in China.

Author

Che, Haiyan, Li, Zhengnan, Zhang, Lei, Wu, Yunfeng, and Luo, Daquan

Subjects
*PHYTOPLASMAS, *STYLOSANTHES, *PHYTOPATHOGENIC microorganisms, *WITCHES' broom disease, *SYMPTOMS
Abstract

In July 2008, Stylosanthes guianensis with witches'-broom symptoms and Stylosanthes capitata with yellows symptoms were observed in Hainan Province, China. From the symptomatic S. guianensis, the phytoplasma-specific 16S rRNA gene fragment of 1.4 kb was amplified by a direct PCR, and a fragment of 16S rRNA gene of 1.2 kb was obtained from the symptomatic Stylosanthes capitata by a nested PCR. Sequencing results and NCBI BLASTn analysis showed that the two phytoplasmas belonged to group 16SrII. Polygenetic analysis and virtual RFLP classified the two phytoplasmas into subgroup 16SrII-A. [ABSTRACT FROM AUTHOR]

Published
2012
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