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Marek's Disease (MD), which can result in neurological damage and tumour formation, has large effects on the economy and animal welfare of the poultry industry worldwide. Previously, we mapped autosomal MD QTL regions (QTLRs) by individual genotyping of an F 6 population from a full-sib advanced intercross line. We further mapped MD QTLRs on the chicken Z chromosome (GGZ) using the same F 6 population, and by selective DNA pooling (SDP) of 8 elite egg production lines. Here we used SDP of the same pools used on GGZ to map autosomal MD QTLRs. Thirty-seven QTLRs were found. Seven of the QTLRs were tested by all sires from the same 8 lines, individually genotyped for QTLR markers. Five of the tested QTLRs were confirmed. Linkage disequilibrium (LD) was calculated for all QTLR markers on the same chromosome, and complex LD blocks were found. Distribution of P and LD values were used to assess the QTLR causative elements. Allele substitution effects were calculated based on both pooled SNP microarray genotypes, and individual genotypes of QTLRs markers. Substantial allele effect and contribution to the phenotypic and genotypic variation were obtained. The results explain part of the MD response, and provide targets for mitigating MD., Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethics approval: Institutional Review Board Statement: The animal study protocol was approved by Hy-Line International Institutional Animal Care and Use Committee guidelines. Hy-Line do not have specific project identification codes. All of these samples had been collected in the past and stored frozen until use. At the termination of the study animals were humanely euthanized under the approval of the Hy-Line IACUC and supervision of licensed veterinarians., (© 2024. The Author(s).)

In the mid-1960s egg production, fertility, and hatchability of broiler breeder females dropped precipitously. Due to disrupted follicle hierarchies and development of the erratic oviposition and defective eggs (EODES) syndrome. EODES was controlled by restricting feed. In the 1990s, another set of problems arose at entry of broiler breeders into lay and characterized by high mortality followed by lower peak lay and reduction in egg and chick production. These problems are induced by even slight over-feeding, and hence we termed it the "Over Feeding Complex" (OFC). We have speculated that OFC is a quasi-EODES condition, induced by the intense selection for increased breast proportion. To test this, we compared, under fast (FF) and slow (SF) release from feed restriction, body composition and reproductive performance of a broiler breeder from year 1980 (B1980) and kept without selection for performance traits since then, to a line hatched in 2000 (B2000). During the first 16 d of lay, feeding treatment had little effect on egg mass or Laying % for the B1980 birds, while for the B2000 birds, SF treatment resulted in significantly greater egg mass and Laying % compared to FF, showing that the OFC indeed manifested in this experiment. However, contrary to hypothesis, follicle hierarchies were normal for both lines under both feeding treatments. To gain further insight into the OFC syndrome, we here report levels of estradiol, testosterone, and progesterone for these line and treatment groups in the time period leading up to and into lay. A significant line × feeding treatment interaction effect was found for estradiol and testosterone, to a lesser extent for progesterone. For all 3 hormones, for B1980 levels 2 to 3 wk post entry into lay were similar and intermediate under FF and SF, but differed significantly for B2000, being much greater under SF than under FF. Thus, the hormonal effects were parallel and may explain the egg mass and Laying % effects of FF and SF in the 2 genetic types.

During the 1990s, various disturbances arose affecting broiler breeder females at entry into lay. These disturbances were associated with even slight overfeeding during release of feed restriction in this critical maturation period. The present experiment was carried out to gain some insight into the causes of these disturbances by comparing the effect of fast (FF) and slow (SF) release from feed restriction at entry into lay in 2 broiler breeder populations: B1980, representing the genetic level of 1980, and B2000, the genetic level of 2000. Under the FF treatment, B1980 entered lay 19.2 d earlier than B2000; this increased to 37.4 d earlier under SF. The B1980 population entered lay at virtually the same mean age for SF and FF, whereas B2000 entered lay 15.7 d earlier under the FF. Body weight at first egg were 2,621 g for the B1980 and 3,591 g for B2000. Differences in BW at first egg between feeding treatments within lines were minor. As a percentage of BW, ovary, oviduct, and follicle weights were the same for B1980 and B2000; breast weight was 14.9% for B1980 and 21.2% for B2000; abdominal fat pad weight was 5.37% for B1980 and 2.67% for B2000. Follicle weight and absolute difference in weight between successive follicles was greater in B2000 than in B1980. It is concluded that body fat content does not limit entry into lay, and that threshold BW for onset of sexual maturity of broiler breeder hens increased by about 1,000 g between 1980 and 2000, indicating a tight association between juvenile growth rate and threshold BW for onset of sexual maturity. It is also concluded that disturbances at entry into lay due to overfeeding are not due to smaller differences between successive follicles in B2000 compared with B1980. There are hints, however, that overfeeding may contribute to these disturbances by decreasing differences between successive follicles.

Avian eggshell quality is an important trait for commercial egg production, as the eggshell is the primary packaging material and antimicrobial barrier for the internal food resource. Strong eggshells are essential to ensure that eggs can reach their final destination without damage. Ovocalyxin-32 (OCX32) is a matrix protein found within the outer layers of the eggshell and in the cuticle. Numerous reports in the literature have identified association between variants in the gene encoding this protein, OCX32, and various eggshell quality traits. Thus, OCX32 is a candidate gene for selection for eggshell traits in commercial poultry populations. Sequencing of exons 2-6 of the OCX32 gene in eight elite brown and white eggshell commercial egg-laying lines revealed 28 SNPs and one SNP/indel. Eighteen of these SNPs were predicted to alter the amino acid sequence of the protein. Clusters of SNPs in complete linkage disequilibrium were found in both exons 2 and 6. A total of 19 different versions or protein-sequence haplotypes of the OCX32 protein were inferred, revealing considerable variation within commercial lines. Genotypes for 13 of the SNPs were determined for 330-1819 individuals per line. Trait association studies revealed a significant effect of OCX32 on shell color in white egg lines and line-specific significant effects on albumen height, early egg weight, puncture score, and yolk weight. Three of the lines showed a significant change in OCX32 frequency over time, indicating selection pressure for certain variants of this gene during the breeding program., (© 2012 The Authors, Animal Genetics © 2012 Stichting International Foundation for Animal Genetics.)

Background: Marek's disease (MD) is a T-cell lymphoma of chickens caused by the Marek's disease virus (MDV), an oncogenic avian herpesvirus. MD is a major cause of economic loss to the poultry industry and the most serious and persistent infectious disease concern. A full-sib intercross population, consisting of five independent families was generated by crossing and repeated intercrossing of two partially inbred commercial White Leghorn layer lines known to differ in genetic resistance to MD. At the F6 generation, a total of 1615 chicks were produced (98 to 248 per family) and phenotyped for MD resistance measured as survival time in days after challenge with a very virulent plus (vv+) strain of MDV., Results: QTL affecting MD resistance were identified by selective DNA pooling using a panel of 15 SNPs and 217 microsatellite markers. Since MHC blood type (BT) is known to affect MD resistance, a total of 18 independent pool pairs were constructed according to family x BT combination, with some combinations represented twice for technical reasons. Twenty-one QTL regions (QTLR) affecting post-challenge survival time were identified, distributed among 11 chromosomes (GGA1, 2, 3, 4, 5, 8, 9, 15, 18, 26 and Z), with about two-thirds of the MD resistance alleles derived from the more MD resistant parental line. Eight of the QTLR associated with MD resistance, were previously identified in a backcross (BC) mapping study with the same parental lines. Of these, 7 originated from the more resistant line, and one from the less resistant line., Conclusion: There was considerable evidence suggesting that MD resistance alleles tend to be recessive. The width of the QTLR for these QTL appeared to be reduced about two-fold in the F6 as compared to that found in the previous BC study. These results provide a firm basis for high-resolution linkage disequilibrium mapping and positional cloning of the resistance genes.

While rearing birds in confinement and at high density are very successful practices for producing poultry meat and eggs, these conditions may promote the spread of infectious diseases. Consequently, the poultry industry places greatemphasis on disease control measures, primarily at the animal husbandry level. The field of genomics offers great promise to complement these current control measures by providing information on the molecular basis for disease, disease resistance, and vaccinal immunity. This briefly summarizes some of our efforts to apply several genomic and functional genomics approaches to identify genes and pathways that confer genetic resistance to Marek's disease (MD), a herpesvirus-induced T cell lymphoma of chickens. By utilizing the "top-down" approach of QTL to identify genomics regions, and integrating it with "bottom-up" approaches of transcript profiling and Marek's disease virus (MDV)-chicken protein-protein interactions, three genes that confer resistance to MD are revealed, plus a number of other positional candidate genes of high confidence. These genes can be further evaluated in poultry breeding programmes to determine if they confer genetic resistance to MD. This integrative genomics strategy can be applied to other infectious diseases. The impact of the genome sequence and other technological advancements are also discussed.

A primary goal in the assessment of structural variation in the avian genome is to understand the relationship of this variation with biodiversity and with biological performance. To develop such knowledge, certain essential tools are needed. One set of tools includes the laboratory techniques used to assess molecular genetic variation. The current time is a transitional one for this field, in that the recently sequenced chicken genome will add significantly to the portfolio of existing methods used to identify molecular markers. To most efficiently discover marker-trait associations, the experimental mapping populations must be appropriately designed and the relevant statistical analyses applied. This paper reviews methods for assessment of molecular markers in poultry and their use in the characterization of avian biodiversity and in studies to identify marker associations with biological traits, including important considerations of population structure and statistical analysis.

Recent studies report a surprisingly high degree of marker-to-marker linkage disequilibrium (LD) in ruminant livestock populations. This has important implications for QTL mapping and marker-assisted selection. This study evaluated LD between microsatellite markers in a number of breeding populations of layer chickens using the standardized chi-square (chi(2')) measure. The results show appreciable LD among markers separated by up to 5 cM, decreasing rapidly with increased separation between markers. The LD within 5 cM was strongly conserved across generations and differed among chromosomal regions. Using marker-to-marker LD as an indication for marker-QTL LD, a genome scan of markers spaced 2 cM apart at moderate power would have good chances of uncovering most QTL segregating in these populations. However, of markers showing significant trait associations, only 57% are expected to be within 5 cM of the responsible QTL, and the remainder will be up to 20 cM away. Thus, high-resolution LD mapping of QTL will require dense marker genotyping across the region of interest to allow for interval mapping of the QTL.

The objective of the current study was to identify QTL conferring resistance to Marek's disease (MD) in commercial layer chickens. To generate the resource population, 2 partially inbred lines that differed in MD-caused mortality were intermated to produce 5 backcross families. Vaccinated chicks were challenged with very virulent plus (vv+) MD virus strain 648A at 6 d and monitored for MD symptoms. A recent field isolate of the MD virus was used because the lines were resistant to commonly used older laboratory strains. Selective genotyping was employed using 81 microsatellites selected based on prior results with selective DNA pooling. Linear regression and Cox proportional hazard models were used to detect associations between marker genotypes and survival. Significance thresholds were validated by simulation. Seven and 6 markers were significant based on proportion of false positive and false discovery rate thresholds less than 0.2, respectively. Seventeen markers were associated with MD survival considering a comparison-wise error rate of 0.10, which is about twice the number expected by chance, indicating that at least some of the associations represent true effects. Thus, the present study shows that loci affecting MD resistance can be mapped in commercial layer lines. More comprehensive studies are under way to confirm and extend these results.

Many large, half-sib sire families are an integral component of chicken genetic improvement programs. These family structures include a sufficient number of individuals for mapping quantitative trait loci (QTL) at high statistical power. However, realizing this statistical power through individual or selective genotyping is yet too costly to be feasible under current genotyping methodologies. Genotyping costs can be greatly reduced through selective DNA pooling, involving densitometric estimates of marker allele frequencies in pooled DNA samples. When using dinucleotide microsatellite markers, however, such estimates are often confounded by overlapping "shadow" bands and can be confounded further by differential amplification of alleles. In the present study a shadow correction procedure provided accurate densitometric estimates of allele frequency for dinucleotide microsatellite markers in pools made from chicken purified DNA samples, fresh blood samples, and frozen-thawed blood samples. In a retrospective study, selective DNA pooling with thawed blood samples successfully identified two QTL previously shown by selective genotyping to affect resistance in chickens to Marek's disease. It is proposed that use of selective DNA pooling can provide relatively low-cost mapping and use in marker-assisted selection of QTL that affect production traits in chickens.

The effects of feed restriction and photoperiod on body weight (WTSXM) and age (ASXM) at onset of mature semen production in males and onset of lay in females of a broiler female line were examined. Feeding treatments were as follows: ad libitum (AL) and quantitative feed restriction (QR). Photoperiod treatments were as follows: open shed (OS), in which the chicks were reared under naturally increasing daylight with supplemental light; dark room (DR), in which chicks were reared under short days, gradually increasing from 6 h of light (L) to 11.5L; and dark room to open shed (DR-OS), in which chicks reared under short days in the dark room were transferred to the open shed at 149 d. Treatment effects were similar in direction in males and females, suggesting similar control of entry into reproduction of the two sexes, although within a given treatment, males matured earlier than females. Effects of feeding and photoperiod treatments were additive. Relative to QR, the AL feeding treatment increased WTSXM by 832 g for males and 1,089 g for females; ASXM was decreased by 15.8 d for males and 9.3 d for females. Relative to OS, the DR photoperiod increased WTSXM by 591 g for males and 513 g for females; ASXM was increased by 17.5 d for males and 26.8 d for females. The DR-OS photoperiod increased WTSXM by 86 and 169 g for males and females, respectively; ASXM was increased by 6.1 d and 4.9 d, respectively. Under DR, the delay in onset of sexual maturity caused by QR was not due to failure to reach threshold body weight or age and, hence, appears to have resulted from the feed restriction itself. A similar delay for QR was found under OS but might have been due to failure to reach threshold body weight. It is speculated that the delay in onset of sexual maturity caused by feed restriction may be an ecological adaptation or, alternatively, a result of nutritional imbalance.

Previous studies indicate that responsiveness to photoperiod (PR) has deteriorated in White Rock heavy breed females and males, and that selection for increased PR in females has a positive effect on egg production. In order to determine whether PR can be affected by selection in males, a two-way selection experiment for high and low PR, as expressed through early and late onset of mature semen production under suboptimal photoperiod, was implemented in White Rock heavy breed males. In each selection line, the selected males in each generation were mated to unselected females of the same line. After four generations of selection, males and females of the two selection lines did not differ significantly in 6-wk body weight. Males of the high-PR line, however, achieved mature semen production 18.9 d earlier (P < 0.01) and at a body weight 587 g lighter (P < 0.01) than the males of the low-PR line. Females of the high-PR line came into lay 25.9 d earlier (P < 0.01) and at a body weight 375 g lighter (P < 0.01) than females of the low-PR line; they also produced 13.9 more eggs over a 14-wk test period (P < 0.01). These results show that it is possible to affect PR by selection in males, with parallel effects on female PR and reproductive performance. This result carries the potential of improving reproductive performance of male and female broiler chickens through mass selection for early sexual maturity among males.

A consensus linkage map has been developed in the chicken that combines all of the genotyping data from the three available chicken mapping populations. Genotyping data were contributed by the laboratories that have been using the East Lansing and Compton reference populations and from the Animal Breeding and Genetics Group of the Wageningen University using the Wageningen/Euribrid population. The resulting linkage map of the chicken genome contains 1889 loci. A framework map is presented that contains 480 loci ordered on 50 linkage groups. Framework loci are defined as loci whose order relative to one another is supported by odds greater then 3. The possible positions of the remaining 1409 loci are indicated relative to these framework loci. The total map spans 3800 cM, which is considerably larger than previous estimates for the chicken genome. Furthermore, although the physical size of the chicken genome is threefold smaller then that of mammals, its genetic map is comparable in size to that of most mammals. The map contains 350 markers within expressed sequences, 235 of which represent identified genes or sequences that have significant sequence identity to known genes. This improves the contribution of the chicken linkage map to comparative gene mapping considerably and clearly shows the conservation of large syntenic regions between the human and chicken genomes. The compact physical size of the chicken genome, combined with the large size of its genetic map and the observed degree of conserved synteny, makes the chicken a valuable model organism in the genomics as well as the postgenomics era. The linkage maps, the two-point lod scores, and additional information about the loci are available at web sites in Wageningen (http://www.zod.wau.nl/vf/ research/chicken/frame&#95;chicken.html) and East Lansing (http://poultry.mph.msu.edu/).

The time course of comb development and estrogen levels were compared in broiler (BX) and layer (LX) females that consumed feed ad libitum (A) or were subjected to quantitative feed restriction (R). The chicks were reared under short photoperiod [(6 h light (L):18 h dark (D)] until 22 wk of age. At this time, photoperiod was increased in one step to 8L:16 D, and then gradually increased until 14L:10D at 34 wk. There was a significant interaction between genetic type and feeding treatment, such that entry into lay of the LX-R and BX-R females was delayed by 1 and 4 wk, respectively, relative to the LX-A and BX-A birds. Mean comb size of LX-A birds began to increase while still under 6L:18D photoperiod; that of the other treatment groups did not begin to increase until photoperiod was shifted to 8L:16D. Comb size of individual LX-A, LX-R, and BX-A birds began to increase about 8 wk prior to individual onset of lay; that of BX-R birds about 11 wk prior to onset of lay. In all groups, estrogen levels remained low until 3 to 4 wk prior to onset of lay, when they showed a sharp increase. Following onset of lay, estrogen levels of all groups remained high. Critical day length of LX-A birds appears to be lower than that of BX-A birds. In addition, feed restriction per se appears to decrease photoperiod responsiveness of BX birds.

An endogenous virus, denoted evA, is present at high frequency in all brown egg layer lines. Using inverse polymerase chain reaction (PCR) based on the viral LTR regions, products were obtained containing cellular sequences 5' and 3' to the viral insertion point. PCR of chicken genomic DNA was carried out, using primers chosen from the 5' and 3' cellular sequences and a primer chosen from either the U3 or U5 portions of the viral LTR. Amplification of DNA from birds that did not carry evA with the primer triplets always gave a single 364 bp reaction product, interpreted as representing the flank-to-flank amplification product. Amplification of DNA from known homozygous or heterozygous evA carriers, with the same primer triplets, always gave both the expected junction product and 364 bp product. Therefore, these primer sequences can be used to distinguish evA carriers from non-carriers but cannot distinguish between homozygous and heterozygous evA carriers.

Effect of photoperiod on age and BW at attainment of mature semen production was examined in males of lines selected for high (HL) and low (LL) threshold BW at onset of lay, and in males of a commercial Cornish male line (MC), a commercial broiler breeder female line cross (BX), and a commercial layer cross (LX). Photoperiod treatments were: I, increasing natural spring light; and D, steadily decreasing artificial light provided in a light-controlled room. Under I, age at onset of mature semen production was 168.5, 133.6, 138.1, 124.0, and 118.0 d for HL, LL, MC, BX, and LX males, respectively. Under D, age at onset of mature semen production increased by 18.3, 8.0, 55.9, 60.9, and -2.0 d, respectively. The differential effect of Treatment D on the various genetic stocks is attributed to differences between stocks in photoperiodic drive. Therefore, the very great increase in age at onset of mature semen production in BX and MC males points to a severe reduction in photoperiodic drive in males of these stocks, and to future problems in male fertility in broiler breeder flocks entering lay in the summer and fall. Lines HL, LL, BX, and LX rank the same with respect to photoperiodic drive, whether measured in males as in this experiment, or in females, as in a previous experiment. This result suggests that genetic factors affecting photoperiodic drive come to similar expression in males and females. Thus, selection for increased photoperiodic drive in broiler line males may be able to increase photoperiodic drive in broiler line females, and in this way improve female reproductive performance.

A two-way selection program, carried out for high and low threshold BW at onset of lay (TWL) resulted in a marked difference in TWL in the selected lines. Later studies showed that the selection procedure had decreased photoperiodic drive in the high line (HL) as compared to the low line (LL), in this way increasing the number of days required from onset of photosensitivity to onset of lay in the HL as compared to the LL. The present study was carried out to determine whether the difference in photoperiodic drive was sufficient to account for the entire difference in TWL between the HL and LL, or whether it is also necessary to postulate a difference in threshold weight for onset of photosensitivity (TWSM) as well. Birds of the two lines were raised under feed restriction to 22 wk reaching weights (1,881.8 g) for LL, 2,304.8 g for HL), well below anticipated TWSM. They were then provided gradually increasing amounts of feed, resulting in daily gains of 3 to 5 g for the LL and 8 to 9 g for the HL. Under this regimen, the HL entered lay an average of 64.4 d later and at a body weight 752.8 g greater than the LL. On the basis of previous studies of these lines, of the 64.4 d delay in onset of lay in the HL as compared to LL, 21 d can be attributed to the difference in photoperiodic drive. The excess of 43 d must thus represent a difference in TWSM. During this period the HL were gaining about 8.5 g/d, and at 22 wk HL body weight was already 153 g greater than LL body weight. In all then, TWSM of HL is estimated at 518 g greater than that of the LL. Thus, the selection procedure employed for TWL apparently affected TWSM as well as photoperiodic drive.

Bulked co-segregant analysis is a method of rapidly allocating unmapped genetic markers to a specific chromosomal region. Although originally developed for utilization in populations derived from crosses between fully inbred lines, it has been proposed that co-segregant pools could also serve the same purpose in outbreeding populations, if individuals from only a single large family are pooled. Large, fully mapped, single-sire backcross and half-sib families are presently available as part of the international chicken and bovine reference family panels respectively. In this study, power and tests of significance for single-parent co-segregant analysis are derived for full-sib, single-parent back-cross and single-parent half-sib families, as a function of proportion of recombination between index marker and linked marker, pro-portion of single-parent alleles among the mates, number of individuals in each segregant pool and technical error variance. Power was found to be greater than 0.80 for many reasonable parameter combinations. The method is illustrated using microsatellite markers and a large single-sire bovine family, part of the international bovine reference family panel.

Selection for high and low threshold weight for onset of lay (ThrWtLay), carried out in a broiler line over six generations, was previously found to increase reproductive performance in the low line (LL). Because of the potential importance of this effect, a two-generation replication of the original experiment was carried out using a modern broiler line. Selection was on the basis of ThrWtLay, measured as body weight at first egg, following gradual release from feed restriction. The total response to selection over two generations was 382 g, giving a realized heritability of .42. The difference in 6-wk body weight of the two lines was +19 g in favor (P > .05) of the high line (HL). Reproductive performance was measured in the S2 generation and in the first (R1) generation of relaxed selection. On the average, age at first egg of LL was 17.6 d earlier, and prepeak, postpeak, and total egg production of LL to a fixed age was 9.9, 1.1, and 11.1 eggs greater, respectively, than that of HL. The differences in age at first egg, prepeak, and total egg production were significant; those in postpeak production were not. There was a difference of 1.1 g in favor (P > .05) of HL for egg weight. Thus, the results of this short-term selection experiment are broadly consistent with those of the original, long-term selection. Namely, ThrWtLay responded strongly to selection, with a powerful accompanied response in age at onset of lay and in prepeak egg production, and only a small effect on 6-wk body weight. However, in contrast to the results obtained previously, LL in the present experiment showed only a small increase (P > .05) in postpeak production relative to HL. Nevertheless, although an effect on postpeak production was not obtained in the present experiment, the results do provide general support for the previously expressed view that selection for early onset of lay, on release from feed restriction, may provide a means of increasing photoperiodic drive and consequent reproductive performance of broiler breeders.

The effects of presence or absence of individual endogenous virus (ev) genes on production traits was studied in a highly productive commercial layer cross. Age and BW at first egg, egg production, egg weight, and mature BW were recorded for each bird. The birds were examined for presence of ev gene fragments by Southern analysis. A general linear model was used to determine significance of effects of the 21 individual ev fragments on the individual traits and the effects of all ev fragments taken together on each of the traits. Seven significant effects were found for individual ev fragments on individual traits. Four of these involved BW at first egg, and all ev gene fragments taken together had a significant effect on BW at first egg, explaining 17% of total phenotypic variation in this trait. Significant nonlinear correlations were found between total number of ev genes and both BW at first egg and mature BW, with birds having a moderate number of ev genes showing the lowest BW. For age at first egg and egg weight, nonlinear correlations, although not significant, were consistent in sign with those found for BW, implying minimum trait magnitude at moderate number of ev genes. These effects imply that animals with intermediate numbers of ev genes will tend to be favored by commercial selection in layer flocks, whereas birds with either too many or too few ev genes will tend to be culled.(ABSTRACT TRUNCATED AT 250 WORDS)

A two-way selection program, carried out for high and low threshold BW at onset of lay (ThrWtLay), decreased photoperiodic drive of the high line (HL) relative to the low line (LL). Photoperiodic drive in modern broiler breeder females was compared to layer females in experiments using the HL and LL lines, a commercial layer cross (LX), and a commercial broiler breeder female line cross (BX). Treatment groups were: NS, natural decreasing light to 20 wk, followed by natural light supplemented to 16 h daily with artificial light; NN, natural decreasing light to end of experiment; DD, dark room from hatch to end of experiment; ND, natural decreasing light to 20 wk, dark room thereafter. Under NS, mean age and BW at first egg was greater for the HL line than for the LL line. The difference increased by a further 8.9 d and 217.5 g under NN. Similarly, under NS, mean age and BW at first egg was greater for BX than for LX, and the difference increased by a further 22.6 d and 256.1 g under NN. These interactions indicate greater photoperiodic drive of LX and LL stocks relative to the HL and BX stocks. Prepeak and peak egg production (EP) was greatest for the LX, lower for the LL, and lower yet for HL and BX. Photoperiodic drive may be of major importance in determining prepeak and peak EP. Except for the DD treatment, age and BW of the LX cross at first egg were virtually identical across treatments, those of the LL line were similar, whereas those of the HL and BX stocks showed differences. Similarly, EP curves of the LX cross were virtually identical across treatments, those of the LL line were relatively uniform, but those of the HL and BX stocks again showed great differences. Standard deviation and coefficient of variation of peak EP were very low for the LX cross, still low, but significantly greater for the LL line, and significantly greater yet again for both HL and BX stocks. Thus, increased photoperiodic drive may also be associated with greater stability of reproductive performance. Selection for early onset of lay on release from feed restriction may provide a means of increasing photoperiodic drive and consequent reproductive performance of broiler breeders.

Two chicken genomic libraries were screened for the presence of poly(TG/AC) microsatellite tracts. The number of positive clones was low, confirming the low frequency of such microsatellites in the chicken genome relative to mammalian genomes. Polymorphism of 29 microsatellite tracts, comprising 11 from the library screening and 18 obtained from GenBank, was examined in the East Lansing and Compton reference families, in a resource population formed by a cross between a single White Rock broiler and inbred Leghorn females, and in a panel of birds from five layer stocks. Twenty microsatellites, primarily of the poly(TG/AC) type, were polymorphic in at least one of the populations. Thirteen of the microsatellites were polymorphic in the East Lansing reference family and 13 were also polymorphic in the resource population, confirming that the genetic distance between White Rock and White Leghorn is about as great as between Jungle fowl and White Leghorn. Only six microsatellites were polymorphic in the Compton reference family, formed by a cross between two White Leghorn strains. Twelve of the microsatellites were mapped in the East Lansing and/or Compton reference families. These were well dispersed among the various linkage groups and did not show any indications of terminal clustering.

In order to undergo the processes leading to lay, female chickens must reach a minimum threshold age and body weight, and be exposed to some minimum amount of light stimulation. By two-way selection over six generations lines were developed that differed markedly in body weight at first egg. The effect of supplemental light on age and body weight at first egg of these lines was studied in two experiments. In the first experiment, birds of the two lines were provided ad libitum access to feed from hatch, reaching the weight threshold at an early age. Supplemental light was provided at either 91 or at 126 days of age (before or after the age threshold, respectively). Differences in body weight and age at first egg of the two lines were the same in both light treatments. In the second experiment, birds of the two lines were raised under feed restriction to 154 days of age and then released to ad libitum feeding under supplemental or natural (autumn) light. Differences between the lines in age and weight at first egg were greater in the group maintained under natural light than under supplemental light. The results are interpreted in terms of a model relating age at first egg to weight and age thresholds for onset of competence to respond to light stimulation, and to cumulative light stimulation required from onset of competence to first egg. It is proposed that selection affected the total amount of light stimulation required from onset of competence to onset of lay, but did not affect weight or age thresholds for onset of competence.

A selection program for high and low threshold body weight at first egg was carried out in a broiler line. Selection was on the basis of weight at first egg, following gradual release from feed restriction at a relatively advanced age. After six generations of selection, the lines differed by 862 g in the trait under selection. In addition, 6-wk body weight of high-line (HL) birds was 91 g greater, and age at first egg was 32 days greater than in low-line (LL) birds. When raised under an ad libitum feeding regimen from hatch, HL birds entered lay 20 days later than LL birds. When onset of lay was markedly delayed by maintaining feed restriction until 29 wk of age, body weight at first egg of HL birds was greater by 645 g than that of LL birds. Similarly, when birds of the two lines were subjected to forced molt and brought to a body weight well below that of initial body weight at first egg, and then allowed to gain weight and reenter lay, body weight difference at first egg of HL and LL birds following rehabilitation was similar to that found on original entry into lay. It is proposed that the results may most plausibly be explained as resulting from a primary effect of the selection procedure on the time required to first egg from onset of sexual competence, defined as onset of responsiveness to lay-inducing factors such as light. Alternative explanations involve effects of the selection procedure on threshold weight or threshold age requirements for sexual competence.

The distribution of endogenous virus (ev) genes was studied in five commercial layer lines; four were brown-egg types and one was White Leghorn. The DNA samples were obtained from nine birds of each line and digested with SacI and BamHI endonucleases. The DNA fragments were separated by gel electrophoresis, and Southern blots were prepared and examined for the presence of ev genes following hybridization with the labeled recombinant plasmid pRAV-2 and autoradiography. Almost all fragments were present in more than one line, suggesting that each line has drawn a random assortment of ev genes from the same common pool, possibly as a founder effect. A great degree of polymorphism is shown by the ev genes in the lines investigated. Most fragments were present at low or intermediate frequencies. Very few fragments were present in only one line or in only one bird. This suggests that de novo integration of ev genes at new sites is a relatively rare event. Very few fragments were present at high frequencies, and none of the fragments, except for the BamHI internal fragments, were present in all birds of all lines. Considering that ev genes are present in the wild progenitor of the domestic chicken, the lack of fixation of any ev genes in the chicken genome is remarkable. It is proposed that ev genes have general deleterious effects in the chicken (hence, the lack of fixation), but those ev genes that have been retained in the chicken genome have favorable effects under some circumstances with consequent equilibrium at low to intermediate frequencies. It is speculated that the retained ev genes may represent sites of favorable mutation via insertional mutagenesis and, hence, a potential route to the cloning of genes of economic importance in poultry.

Genetic and phenotypic variation and covariation in immune response to inactivated Newcastle disease virus (NDV) vaccine and to Escherichia coli vaccine were studied in commercial poultry strains. Within any given experiment there was no tendency for individual birds to respond in a correlated manner to NDV and E. coli vaccines. There were highly significant differences between sire families in immune response to NDV vaccine (57 sire families) and to E. coli vaccine (35 sire families). Heritabilities of immune response levels to NDV and to E. coli were .41 and .25, respectively. In both cases, additive genetic standard deviations were slightly over 1.0 titer unit. The correlation between sire-family means for response to NDV and sire-family means for response to E. coli (35 sire families) was .077 and statistically nonsignificant. Thus, the data provide evidence for the presence of significant genetic variation in immune response with respect to two endemic disease antigens, but they provide no evidence for a genetic correlation in response to the two antigens.

The heritability of minimum weight for onset of sexual maturity in chickens (WTSXM) and its phenotypic and genetic correlations with early growth rate were studied in a Leghorn (L) layer stock, in two White Rock (WR) broiler stocks, and in the F4, F5, and F6 segregating generations of a White Rock X Leghorn (WR X L) cross. Mean heritability of WTSXM was .38 in the WR populations and .84 in the WR X L cross populations. Mean WTSXM of the various genetic stocks were closely related to their mean early growth rate. The mean phenotypic correlation of WTSXM and early growth rate was .07 in the WR populations but .47 in the WR X L cross populations. Estimates of the genetic correlation of WTSXM with early growth rate were high, and uniform in sign in the WR X L cross populations but smaller in magnitude and variable in sign in the WR populations. The implications of these results with respect to WTSXM as a breeding objective are discussed.

1. Compensatory growth and the onset of lay were studied in White Rock broiler females severely restricted in food intake from day of hatching. 2. When changed to feeding ad libitum the restricted birds gained weight rapidly, but stabilised at a mean body weight 500 to 700 g less than that of controls fed ad libitum. Egg production was similar to that of controls. 3. Birds maintained at low mean body weight ( 2 224 g at 42 weeks) by continued restriction did not come into lay. In groups maintained at somewhat higher mean body weights (2 300 to 2 700 g) for period of up to a year, some birds came into lay. 4. Birds fed ad libitum showed accelerated development of comb, ovary and oviduct and weighed 2 300 to 2 700 g between 14 and 15 weeks of age, but did not come into lay until 20.5 weeks of age. 5. These results suggest that a minimum body weight and age may be required for the onset of lay in this strain.

The effect of diet and feed restriction on minimum weight for onset of sexual maturity was studied in White Rock broiler breeders. In the restriction experiment, three groups of birds were subjected to moderate, severe, and very severe quantitative food restriction during the rearing period (19 week target weights: 1900, 1300, and 700 g, respectively). From 20 to 24 weeks body weight was equalized at 2200 g, and from 24 weeks birds were allowed to gain about 150 g/week until first egg. In the diet experiment, two groups of birds were raised under severe quantitative food restriction during the rearing period and then allowed to gain about 150 g/week on a low metabolizable energy/protein (ME/P) ratio diet (25.4% protein, ME/P = 116) and on a high ME/P ratio (14.7% protein, ME/P = 219) until first egg. All birds were killed at first egg. Birds of the three restriction groups entered lay at the same lean body weight and ash and protein content, but at different age, carcass weight, shank length, dry matter, and fat content. Birds of the two diet groups entered lay at the same lean body weight, shank length, and ash content but at different carcass weight, dry matter, and fat content. Considering both experiments together, birds of the different treatment groups entered lay at similar lean body weight and ash content but differed in age, carcass weight, shank length, dry matter, and fat content.(ABSTRACT TRUNCATED AT 250 WORDS)

Immune response to Newcastle disease virus (NDV) vaccine, fowl pox, and E. coli vaccine was compared in the native Bedouin fowl of the Sinai desert, in a commercial Leghorn layer strain, and in the reciprocal crosses between them. Differences were not found in antibody titer levels to attenuated or inactivated NDV vaccines, in the proportion of birds showing post-vaccination immunity to fowl pox, or in the kinetics of postvaccination NDV titer levels. Rate of development of titer to Escherichia coli from day 1 to day 4, however, was significantly more rapid in Bedouin chicks than in the purebred Leghorn or the reciprocal crosses.

Genetic differences in immune response to Newcastle disease virus (NDV) were studied in 4-week-old broilers, vaccinated with attenuated (live) or inactivated NDV. The experiment included 370 chicks from two farms distributed among 22 sire families and 60 dam families. Results in chicks from both farms were similar. Survival after challenge was closely related to titer level. The genetic correlation between day-7 and day-12 titers (attenuated virus) was 1.0. Significant differences were found between sire families in both sorts of vaccinations. Heritabilities based on the sire variance components for attenuated and inactivated virus vaccinations were respectively 0.31 and 0.60. The genetic correlation between them was 0.49. Nevertheless, it is concluded that selection for response to NDV based on inactivated virus may be most effective in improving response to attenuated NDV vaccinations.