1. UV-B Induced stimulation of phosphatidylinositol 3-kinase in human fetal hepatocytes
- Author
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Khaja Khaleel Ur. Rahman, M. Raghuveer Singh, Sathish Kasina, R. S. R. Gaddameedi, C. M. Habibullah, V. B. Chari, and Surya S. Singh
- Subjects
Kinase ,Clinical Biochemistry ,Lipid kinase activity ,Biology ,Molecular biology ,Article ,Wortmannin ,Transplantation ,chemistry.chemical_compound ,chemistry ,Biochemistry ,LY294002 ,Viability assay ,Phosphatidylinositol ,Intracellular - Abstract
We investigated the effect of UV-B irradiation on PI 3-kinase activity in human fetal hepatocytes. When cells were exposed to monochromatic (304nm) UV-B light, a significant increase in intracellular PI 3-kinase activity was observed in a dose dependent manner with maximal activity upon 1500 Jm(-2) irradiation. At 1500 Jm(-2) dose PI 3-kinse activity increased by 80% in membrane fraction of fetal hepatocytes of 25 weeks gestation. PI 3-kinse inhibitors wortmannin and LY294002 specifically inhibited the UV-B induced lipid kinase activity and blocked significantly the UV-B induced cell viability. The data suggests a correlation between cell survival and elevated levels of PI 3-kinase and suggest that UV-B irradiation at a dose of 1500 Jm(-2) is ideal for fetal hepatocyte transplantation. Also, PI 3-kinase levels could be a representative marker for viable UV-B irradiated fetal hepatocytes for transplantation.
- Published
- 2004
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