1. Liquid chromatography–tandem mass spectrometry method for the analysis of N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, a biocidal disinfectant, in dairy products
- Author
-
Jean-Pierre Abjean, Dominique Hurtaud-Pessel, Kahina Slimani, Pierre Maris, Yvette Pirotais, and Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
- Subjects
désinfectant ,Analyte ,analysis ,Electrospray ionization ,analyse ,Mass spectrometry ,01 natural sciences ,Analytical Chemistry ,Matrix (chemical analysis) ,residues ,chemistry.chemical_compound ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Cheese ,Limit of Detection ,Tandem Mass Spectrometry ,Liquid chromatography–mass spectrometry ,Chromatographie liquide couplée à la spectrométrie de masse ,animal ,résidus ,Acetonitrile ,ComputingMilieux_MISCELLANEOUS ,diamine ,Detection limit ,milk ,Chromatography, Reverse-Phase ,Chromatography ,Propylamines ,010405 organic chemistry ,010401 analytical chemistry ,Selected reaction monitoring ,produits laitiers ,biocide ,General Medicine ,lait ,0104 chemical sciences ,food safety ,veterinary medicine ,dairy product ,chemistry ,Liquid chromatography-tandem mass spectrometry ,Dairy Products ,disinfectant ,Disinfectants ,Food Science - Abstract
A novel and reliable method to quantify residual levels of N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine in dairy products using ion-pairing reversed-phase liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed and fully validated. Sample extraction was done with salting-out technique using acetonitrile and sodium chloride. For LC–MS/MS, the analyte was detected using positive electrospray ionization (ESI+) and two multiple reaction monitoring (MRM) transitions were monitored. The method was validated in the 5–150 µg kg−1 range using total error approach. Thus, performance criteria of the method were evaluated. Relative standard deviations for trueness and precision were lower than 10%; with the exception of hard pressed cheese at 5 µg kg−1 for precision. The limit of quantification (LOQ) was around 5–7 µg kg−1 depending on the matrix of interest. The method was successfully applied to accurately quantify N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine in 146 various dairy products with a maximum contamination level of 225 µg kg−1 in cheese.
- Published
- 2018
- Full Text
- View/download PDF