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Nitrate is the principal source of nitrogen for most higher plants. Particularly leafy vegetables require large quantities of nitrate fertilizer to attain maximal yields. The crop yield and quality as well as nitrate accumulation in leafy vegetables depends upon many preharvest factors such as genetic materials, agronomical and environmental factors. Three greenhouse experiments were carried out in order to assess the effects of preharvest factors (cultivars, plant density and N dose) on yield and quality of spinach (Spinacia oleracea L.). In the first trial four spinach cultivars were compared in terms of yield and quality attributes. The marketable yield of the four cultivars tested was in the following order: ‘Tasman’ = ‘Kookaburra’ > ‘Platypus’ = ‘Regiment’. No significant differences among cultivars were observed for the βcarotene, vitamin C and nitrate contents. In the second trial, treatments were defined by four plant densities (800,000; 1,000,000; 1,200,000; 1,500,000 seeds ha-1). The lowest marketable yield was observed under the lowest plant density, whereas no significant difference was recorded between the other treatments. The highest leaf dry matter, K, and nitrate contents were recorded at 1000000 seeds ha-1. In the third experiment we evaluated the effects of four N fertilizer doses (0, 75, 150 and 225 kg ha-1) on crop performance. Marketable yield increased quadratically with increasing N application with the highest values recorded at both 150 and 225 kg ha-1 indicating a luxury consumption of the nutrient at 225 kg ha-1. Finally, the highest protein and nitrate contents were observed at 225 kg ha-1 of N.

Research background. Numerous factors affect the ripening of reduced-fat white cheese. The aim of this study is to investigate the influence of ripening environment (brine or vacuum plastic bags without brine) on the chemical composition, protein, fatty acid profile and mineral content as well as antioxidant properties of industrially produced reduced-fat white cheese. Experimental approach. A low-fat white cheese was manufactured on an industrial scale from milk that remained after the production of kajmak and ripened for 60 days at 4 °C after packaging in a polystyrene container with brine containing 6 % salt or in vacuum-sealed polyethylene bags. The influence of ripening environment on proteolysis was monitored by the change of soluble nitrogen fractions as well as by sodium dodecyl sulphate–polyacrylamide gel electrophoresis of Tris(hydroxymethyl) aminomethane-HCl extracts of cheese proteins under non-reducing conditions and water-soluble fractions under reducing conditions. An effect that ripening environment had on fatty acid and mineral content was also monitored. The change of antioxidant potential of the investigated cheese during ripening led to the change of iron(II) chelating ability, reducing power and free-radical scavenging activity. Results and conclusions. The ripening environment differently affected proteolysis, fatty acid composition, mineral profiles and antioxidant properties of reduced-fat white cheese. White cheese ripened in brine had more intensive proteolytic changes than the cheese ripened in a vacuum, but also more intensive diffusion processes, especially between the 40th and 60th day of ripening. The brine-ripened cheese had higher values of water-soluble nitrogen content, but lower contents of trichloroacetic acid-soluble and phosphotungstic acid-soluble nitrogen than a vacuum ripened cheese. Cheese ripened in brine had a lower content of almost all investigated macro- and microelements. After 60 days of ripening, in the cheese ripened in brine only myristic (C14:0) and palmitic acid (C16:0) were detected, whereas in a vacuum-ripened cheese C10:0-C16:0 fatty acids dominated. Vacuum-ripened reduced-fat cheese had more favourable reducing power, while white brined reduced-fat cheese had better radical scavenging activity and iron(II) chelating activity. Novelty and scientific contribution. These results suggest significant influence of ripening conditions (immersion in brine or in vacuum-sealed polyethylene bags) on nutritive and functional properties of reduced-fat white cheese. Ripening in a vacuum has become a useful method for obtaining high-value reduced-fat white cheese.

In the last twenty years, horticulture has focused on producing high-quality food while improving resource use efficiency. The application of nutritional stress is an effective tool to increase the phytochemical content of vegetables while reducing environmental footprint. Microgreens represent an emerging functional food characterized overall by higher levels of phytonutrients than their mature counterparts. The current study examined the effect of using nutrient solution or only distilled water on the nutraceutical performance of lettuce (Lactuca sativa L.) microgreens grown in a peat-based substrate. Our results showed that lettuce microgreens can be effectively grown using only substrate nutrients, incurring a 27% decrease in fresh yield, but a significant increase in total ascorbic acid (+187%), anthocyanins (+35%), and total phenolic acids content (+26%). Of utmost interest, is the near absence of nitrates in water-treated microgreens, which renders this category of fresh vegetables ideal for the production of food dedicated to consumers highly sensitive to nitrates, such as infants. Growing microgreens without providing additional nutrients to those found in a typical peat-based substrate is an important potentiality that adds value to this new class of vegetables and also renders them particularly suitable for home cultivation.

Oats are increasingly recognized as super food owing to the presence of multiple health promoting substances. Five indigenous oat cultivars S2000, PD2LV65, SGD2011, Avon and SGD81 were characterized physico-chemically and nutritonally. Avon showed the maximum (12.69 g100 g-1 dry solids) protein content indicating potential to be used as protein source for animal and human food, whereas SGD-81 excelled incrude fiber contents (17.83 g100 g-1 dry solids). Avon showed the highest fat contents (6.67 g100 g-1 dry solids). Maximum moisture contents showed by the SGD-81 (9.29 g100 g-1 dry solids). SGD-2011 showed maximum ash contents (6.02 g100 g-1 dry solids). S-2000 showed the maximum nitrogen-free extract (60.78 g100 g-1 dry solids) and thousand grain weight (36.74 g). Highest Zn (0.176 mg g-1 of dry solids) and Fe (0.032 mg/g-1 dry solids) contents were found in Avon. Avon showed maxium bulk density of 0.76 g/cc and Avon grains had maximum length and breadth ratio (2.93). The highest water absorption capacity, oil absorption capacity and emulsion stability were shown by SGD-81 as 188.33%, 218.754% and 73.13%, respectively. The highest foaming capacity was shown by S-2000 (20%) and the highest emulsion activity was shown by SGD 2011 (56.84%). Amino acid profiling showed the highest outcome 105.67 g kg−1 of dry solids by SGD-2011.While least 99.13 g kg−1 of dry solids depicted by S2000. Total phenolic contents varied from 36.07 to 59.6 mg of gallic acid equivalent (GAE), total flavonoids (754.16–1056.66 mg of quercetin equivalent:QE), TfoC (663.75–697.5 mg QE), % scavenging activity (24.33–55.88%) and anthocyanin contents of 0.5–2.87 mg of C3 G kg−1. SGD-81 was loaded with promising nutraceutical potential. SGD-81 would be good substitute of fiber and also loaded with other promising functional attributes including total flavonoid contents, total phenolic contents, total flavonol conetnts and anthocyanin contents. There is great potential for value addition of the oat cultivar SGD-81. Abbreviation NFE: Nitrogen-Free Extract; WAC: Water absorption capacity; NFE: Nitrogen-Free Extract; OAC: Oil absorption capacity; TGW: Thousand grain weight; FC: foaming capacity; BD: Bulk density; EA: Emulsion activity; L/B ratio: Length and breadth ratio; TFC: Total flavonoid contents; TFoC: Total flavonol contents; DPPH-2,2-Diphenyl-1-Picrylhydrazyl; NAA: Nonessential amino acid; TAA: total amino acids; ES: Emulsion stability; TPC: Total phenolic contents; ppm: parts per million; EAA: Essential amino acids; mg of C3G kg−1 -milligram of Cyanidine 3-glucoside per kilogram

Honey and all bee products have very good biological and chemical properties. They have been used in medicine for a long time. In our study we evaluated twenty polyfloral honey samples, the first ten were commercial honeys from a selected supermarkets with country of origin indicated "blend of EU and non-EU honeys" and represented imported honey. The second ten honeys were from a local beekeepers (Nitra region) and represented the Slovak origin. The aim of the study were to analyze chemical, microbiological, antioxidant, antimicrobial profile of honey and recommend marketing strategies for honey producers by applying nutrition marketing. From chemical point of view, the study examined mineral profile of honeys, antioxidant properties as antioxidant activity, total polyphenols, flavonoid and phenolic acid content and from microbiological view the study evaluated a total count bacteria, coliform bacteria and microscopic filamentous fungi. Results of minerals showed that the most dominant element in commercial honeys is sodium (30 mg.100g-1) followed by calcium, potassium, magnesium and phosphorus. Iron, arsenic and selenium are present only in trace amounts. In local honeys the most dominant element is potassium (84.181 mg.100g-1) followed by calcium, phosphorus, sulfur and magnesium. The presence of hazardous heavy metals (cadmium, lead and chrome) was not detected in either of the samples. Moreover, antioxidant activity determined by the DPPH method was slightly higher in local polyfloral honeys and vice versa the content of total polyphenol, flavonoid and the phenolic acid content was slightly higher in commercial polyfloral honeys. From the microbiological point of view, the total count of bacteria was found only in commercial polyfloral honeys while local honeys were without detectable microorganisms. The best antimicrobial activity was found against gram-negative bacteria such as Escherichia coli in both concentrations of honeys, and the local honeys reached better antimicrobial activity. All in all, honey has very good biological properties and mineral composition which opens opportunities for beekeepers to apply nutrition marketing and target new segments of consumers, e.g. sportsmen, people in convalescence, consumers following healthier lifestyle or seeking functional food. Moreover, educating consumers from a nutritional point of view will foster daily intake of honey and will increase annual consumption of honey in the future.

According to the Regulation No. 1831/2003 of the European Parliament and European Union Council, the use of antibiotics as a dietary supplements has been prohibited. It seems that the administration of prebiotics, instead of antibiotics, into the pig's diet, may regulate the intestinal microbiota and has a long-term health-related impact on the host. Inulin-type fructans can stimulate mineral absorption from the gut. Additionally, it may regulate energy metabolism and activate enzymatic mechanisms preventing oxidative stress. The goal of the present study was to estimate the influence of dietary supplementation with dried chicory root or native chicory inulin on 1) liver histology; 2) liver and kidney lipid metabolism indices, activity of selected enzymes, concentration of macro- and micronutrients and heavy metals; 3) blood plasma, liver and kidney oxidative stress biomarkers and 4) blood plasma water-electrolyte homeostasis indices in growing pigs. The nutritional study was conducted on 24 piglets assigned to 3 dietary groups (n = 8): control (C) fed a basal diet and two experimental groups receiving basal diet supplemented with 2% of inulin (IN) either 4% of dried chicory root (CR). The animals were fed with a group-specific diets for 40 days and then subjected to euthanasia. Subsequently, blood, liver and kidney samples were harvested for further processing. In the control and experimental groups, no apparent morphological abnormalities in the liver tissues were seen. The percent of periodic acid Schiff positive glycogen liver cells was significantly lower in the CR group as compared to C and IN groups (P < 0.001). Chicory root supplementation improved blood plasma prooxidative-antioxidative balance - PAB (P < 0.001) and liver PAB (P < 0.01) and thiobarbituric acid reactive substances - thiobarbituric acid-reactive substances (P < 0.05). Feeding the CR diet increased calcium (P < 0.001) and potassium (P < 0.05) and decreased cadmium (P ≥ 0.05) content in the liver when compared to the C group. Administration of the CR and IN diets increased selenium (Se) and sodium concentrations, whereas decreased zinc content both in the liver (P < 0.01; P < 0.05 and P < 0.05, respectively) and in the kidney (P < 0.01; P < 0.001 and P < 0.001, respectively) of pigs. Additionally, a higher concentration of lead (P < 0.05) was observed in the kidney of pigs fed the CR diet. In conclusion, both dietary supplements had a potential to significantly improve the Se status and oxidoreductive homeostasis in growing pigs.

Foliar application of a vegetal-derived protein hydrolysate as a biostimulant was assessed for possible interaction with the ripening of diploid watermelon grafted onto interspecific hybrid rootstock. Assessment encompassed crop performance, fruit morphometric and sensory quality traits, soluble carbohydrates, macrominerals, and bioactive composition at 10, 20, 30, 40, and 50 days post anthesis (dpa). The biostimulant effect on yield components was confounded by the vigorous rootstock effect. Pulp firmness declined precipitously with cell enlargement from 10 to 30 dpa, and the biostimulant phyto-hormonal potential on firmness and rind thickness was masked by grafting. Pulp colorimetry was determined solely by ripening and peaked at 40 dpa. The biostimulant effect reduced lycopene content by 8% compared to the control. Total sugars coevolved with soluble solids content, peaked at 30 dpa, and then stabilized. Fructose and glucose prevailed during rapid fruit growth from 10 to 30 dpa and sucrose prevailed at advanced ripeness between 40–50 dpa, whereas acidity peaked at 20 dpa and then decreased. Potassium, which was the most abundant micromineral, peaked before full ripeness at 30 dpa. The biostimulant effect on the watermelon fruit ripening process is not granted, at least regarding the conditions this study was carried out under. The absence of biostimulant effect might relate to rootstock vigorousness, the grafted watermelon physiology, or the type of biostimulant used.

Magnesium (Mg) is essential for many biological processes in plant cells, and its deficiency causes yield reduction in crop systems. Low Mg status reportedly affects photosynthesis, sucrose partitioning and biomass allocation. However, earlier physiological responses to Mg deficiency are scarcely described. Here, we report that Mg deficiency in Arabidopsis thaliana first modified the mineral profile in mature leaves within 1 or 2 days, then affected sucrose partitioning after 4 days, and net photosynthesis and biomass production after 6 days. The short-term Mg deficiency reduced the contents of phosphorus (P), potassium, manganese, zinc and molybdenum in mature but not in expanding (young) leaves. While P content decreased in mature leaves, P transport from roots to mature leaves was not affected, indicating that Mg deficiency triggered retranslocation of the mineral nutrients from mature leaves. A global transcriptome analysis revealed that Mg deficiency triggered the expression of genes involved in defence response in young leaves., SCOPUS: ar.j, info:eu-repo/semantics/published

In the Mediterranean region, artichoke and broccoli are major crops with a high amount of by-products that can be used as alternative feedstuffs for ruminants, lowering feed costs and enhancing milk sustainability while reducing the environmental impact of dairy production. However, nutritional quality of milk needs to be assured under these production conditions and an optimal inclusion ratio of silages should be determined. This work aimed to evaluate the effect of three inclusion levels (25%, 40%, and 60%) of these silages (artichoke plant, AP, and broccoli by-product, BB) in goat diets on milk yield, composition, and mineral and fatty profiles. Treatments with 60% inclusion of AP and BB presented the lowest milk yield. No differences were found on the milk mineral profile. Inclusion of AP in the animals&rsquo, diet improved the milk lipid profile from the point of view of human health (AI, TI) compared to BB due to a lower saturated fatty acid content (C12:0, C14:0, and C16:0) and a higher concentration of polyunsaturated fatty acids (PUFA), especially vaccenic acid (C18:1 trans11) and rumenic acid (CLA cis9, trans11), without any differences with the control treatment.

The current research elucidated the agronomical, physiological, qualitative characteristics and mineral composition of lettuce (Lactuca sativa L. var. longifolia) after treatments with a beneficial fungus Trichoderma virens (TG41) alone or in combination with a vegetal biopolymer-based biostimulant (VBP, &lsquo, Quik-link&rsquo, ). The experiment consisted of lettuce plants grown in three N conditions: sub-optimal (0N kg ha&minus, 1), optimal (70N kg ha&minus, 1), and supra-optimal (140N kg ha&minus, 1) N levels. Lettuce grown under 0N fertilization showed a significant increase in fresh yield when inoculated with TG41 alone (45%) and a greater increase with TG41 + VBP biostimulant (67%). At 48 days after transplanting, both the TG41 alone or TG41+VBP biostimulant induced higher values of CO2 assimilation in comparison to the control. The mineral concentrations in leaf tissues were greater by 10% for K and 12% for Mg with the TG41+VBP treatments compared to the untreated lettuce. The lettuce plants receiving either TG41 alone or TG41+VBP biostimulants had a significantly lower nitrate content than any of the untreated controls. In non-fertilized conditions, plants treated with TG41+VBP biostimulants produced lettuce of higher premium quality as indicated by the higher antioxidant activity, total ascorbic acid (+61%&ndash, 91%), total phenols (+14%) and lower nitrate content when compared to the untreated lettuce.

WOS: 000393087300004, A total of 800 day-old broiler chicks were assigned to four test diets to assess the efficacy of phytase, fermented from Aspergillus niger, and boron, as boric acid, on the growth performance and mineral profile in chickens. The dietary treatments included the basal diet and the basal diet supplemented with boron (20 mg/kg), phytase (500 phytase units/kg diet), or a combination of boron and phytase. The following parameters were measured: growth performance indices, serum biochemicals, ash and the mineral profile of the tibia, breast muscle, liver and excreta. Boron decreased the overall feed conversion ratio by 1.9% (P0.05). The performance indices were not significantly influenced by dietary regimens for the first 21 days of the experiment (P>0.05). The addition of boron alone and boron + phytase resulted in significant increases in the boron concentrations of serum, bone, breast muscle, liver and excreta (P0.05). The chickens administered a diet with boron excreted less Cathrough excreta. However, the ash content and Fe and Cu concentrations in the excreta increased in response to phytase supplementation (P

Plant-derived protein hydrolysates (PHs) are gaining prominence as biostimulants due to their potential to improve yield and nutritional quality even under suboptimal nutrient regimens. In this study, we investigated the effects of foliar application of a legume-derived PH (0 or 4 ml L&minus, 1) on greenhouse baby spinach (Spinacia oleracea L.) under four nitrogen (N) fertilization levels (0, 15, 30, or 45 kg ha&minus, 1) by evaluating morphological and colorimetric parameters, mineral composition, carbohydrates, proteins, and amino acids. The fresh yield in untreated and biostimulant-treated spinach plants increased in response to an increase in N fertilization from 1 up to 30 kg ha&minus, 1, reaching a plateau thereafter indicating the luxury consumption of N at 45 kg ha&minus, 1. Increasing N fertilization rate, independently of PH, lead to a significant increase of all amino acids with the exception of alanine, GABA, leucine, lysine, methionine, and ornithine but decreased the polyphenols content. Interestingly, the fresh yield at 0 and 15 kg ha&minus, 1 was clearly greater in PH-treated plants compared to untreated plants by 33.3% and 24.9%, respectively. This was associated with the presence in of amino acids and small peptides PH &lsquo, Trainer&reg, &rsquo, which act as signaling molecules eliciting auxin- and/or gibberellin-like activities on both leaves and roots and thus inducing a &ldquo, nutrient acquisition response&rdquo, that enhances nutrients acquisition and assimilation (high P, Ca, and Mg accumulation) as well as an increase in the photochemical efficiency and activity of photosystem II (higher SPAD index). Foliar applications of the commercial PH decreased the polyphenols content, but on the other hand strongly increased total amino acid content (+45%, +82%, and +59% at 0, 15, and 30 kg ha&minus, 1, respectively) but not at a 45-kg ha&minus, 1-rate. Overall, the use of PH could represent a sustainable tool for boosting yield and nitrogen use efficiency and coping with soil fertility problems under low input regimens.

Plant biostimulants (PBs) such as protein hydrolysates and seaweed extracts are attracting the increasing interest of scientists and vegetable growers for their potential toenhance yield and nutritional quality. The current study assessed crop productivity, leaf colorimetry, mineral profile and bioactive compounds of greenhouse spinach in response to the foliar application of three PBs: legume-derived protein hydrolysate [PH], extract of seaweed Ecklonia maxima or mixture of vegetal oils, herbal and seaweed Ascophyllum nodosum extracts. Plants were PB-treated at a rate of 3 mL L&minus, 1 four times during their growth cycle at weekly intervals. Foliar PB applications enhanced fresh yield, dry biomass and leaf area of spinach in comparison with untreated plants. Improved yield performance with PB applications was associated with improved chlorophyll biosynthesis (higher SPAD index). The three PB treatments elicited an increase in bioactive compounds (total phenols and ascorbic acid), thus raised the functional quality of spinach. The application of PH enhanced K and Mg concentrations and did not result in increased nitrate accumulation as observed with the other two PB treatments. Our findings can assist vegetable farmers and the agro-food industry in adopting innovative and sustainable tools such as PB for complementing a high yield with premium quality.

The aim of this study was to assess the effects of two levels of clinoptilolite administered in feed (2% and 4%) on some selected performance indicators, metabolic utilization of basic nutrients and the health status of laying hens. The selected 24 Bovans Goldline hybrid laying hens were divided into three equal groups, two experimental groups (E1 and E2) and one control group (C). The laying hens were housed individually in cages with an automatic supply of drinking water, manual feeding, in a setting with controlled light and temperature regimens. Hens from individual groups were all fed a complete feed mix of the same composition and the only difference was in clinoptilolite supplementation: feed mixes for E1 and E2 groups contained 2% and 4% of clinoptilolite (commercial additive ZeoFeed) respectively, replacing the same amounts of wheat. The hens received feed mixes and drinking water ad libitum. During this 28-day experiment, feed consumption and the number and weight of eggs laid were monitored individually for each hen. At the end of the experiment, the balance test using the indicator method (Cr2O3) was performed in all eight hens in each of the groups. The results of balance tests were then used to calculate the metabolic utilization of selected nutrients (nitrogen, fat, ash, nitrogen-free extracts, starch, gross energy, Ca, P). After the balance tests, blood samples for haematological and biochemical examinations were collected via puncture of the vena basilica. The addition of 2% clinoptilolite to feed mix resulted in a highly significant (P ⪬ 0.01) increase in mean egg weight to 64.69 g, but the addition of 4% clinoptilolite in group E2 resulted in a highly significant (P ⪬ 0.01) decrease in mean egg weight to 62.20 g compared to the control (63.73 g). Moreover, daily feed mix consumption in group E1 decreased to 114 g per one laying hen/day compared to the controls (118 g per one laying hen/day). In group E2 (4% clinoptilolite), daily consumption of feed mix increased compared with the controls to 124 g. The 2% clinoptilolite supplementation of E1 group feed slightly increased metabolic utilization of fat, nitrogen-free extracts, starch and gross energy compared to group C. Results of haematological test of the hens’ blood showed significant changes in haemoglobin, whose values in groups C and E1 were significantly higher (P ⪬ 0.05) than in group E2. Differences in the values of the biochemical indicators monitored (total protein, glucose, cholesterol, triacylglycerols, calcium, phosphorus, magnesium and the AST enzyme) between group C and experimental groups E1 and E2 were not significant and remained within the range of reference values.

SCOPUS: ar.j, info:eu-repo/semantics/published