1. Antiplatelet activity and chemical analysis of leaf and fruit extracts from Aristotelia chilensis
- Author
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Eduardo Fuentes, Benita González, Mauricio Mastrogiovanni, Hermine Vogel, Andrés Trostchansky, Iván Palomo, Lyanne Rodríguez, Mário Roberto Maróstica Júnior, and Irene Wood
- Subjects
0106 biological sciences ,Leaves ,Platelet aggregation ,Platelet Aggregation ,Physiology ,Elaeocarpaceae ,Plant Science ,030204 cardiovascular system & hematology ,Antiplatelet Therapy ,01 natural sciences ,Biochemistry ,Anthocyanins ,Domestication ,chemistry.chemical_compound ,0302 clinical medicine ,Reactive oxygen species formation ,Animal Cells ,Tandem Mass Spectrometry ,Medicine and Health Sciences ,Food science ,Chile ,Chromatography, High Pressure Liquid ,Multidisciplinary ,biology ,Pharmaceutics ,Plant Anatomy ,food and beverages ,Eukaryota ,Hematology ,Plants ,Body Fluids ,Chemistry ,P-Selectin ,Blood ,Physical Sciences ,Medicine ,Platelet Granule ,Anatomy ,Cellular Types ,Research Article ,Platelets ,Fruit extracts ,Science ,Research and Analysis Methods ,Fruits ,03 medical and health sciences ,Aristotelia chilensis ,Drug Therapy ,Phenols ,Humans ,Molecular Biology Techniques ,Blood Coagulation ,Molecular Biology ,Blood Cells ,Plant Extracts ,Tetraspanin 30 ,Organisms ,Chemical Compounds ,Biology and Life Sciences ,Proteins ,Polyphenols ,Cell Biology ,biology.organism_classification ,Plant Leaves ,chemistry ,Anthocyanin ,Fruit ,Collagens ,Platelet Aggregation Inhibitors ,010606 plant biology & botany ,Cloning - Abstract
Aristotelia chilensis (Mol.) Stuntz, also known as maqui, is a plant native to Chile without chemical characterization and quantification of the bioactive compounds present in it. HPLC-UV and HPLC-MS/MS studies have shown the presence, at different concentrations, of phenolic and anthocyanin compounds in fruit and leave extracts of the domesticated maqui clones Luna Nueva, Morena, and Perla Negra. The extracts from leaves and unripe fruits of Luna Nueva and Morena clones significantly inhibit platelet aggregation induced by several agonists; the extracts inhibit platelet granule secretion by decreasing the exposure of P-selectin and CD63 at the platelet membrane. Reactive oxygen species formation in platelets is lower in the presence of maqui extracts. Statistical Pearson analysis supports the levels of phenolic and anthocyanin compounds being responsible for the antiaggregant maqui effects. This work is the first evidence of antiplatelet activity from Aristotelia chilensis giving added value to the use of leaves and unripe fruits from this species.
- Published
- 2021