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Breast cancer is one of deadliest diseases in the world. Turmeric extract was known to have antiproliferative activity. To minimize its toxicity, turmeric extract was encapsulated with liposome, a vesicle lipid bilayer that was functioned as cancer drug carrier in body. This research aimed to determine encapsulation effect of turmeric extract against antiproliferative activity in T47D breast cancer cells through in vitro assay. Liposomes was made using thin layer method and particle size was reduced by extrusion. Materials that was used phosphatidylcholine, cholesterol, and turmeric extract. Optimization of liposomes was made in three formulations with different extract concentrations. The most optimal formulation was formulation with less extract and physical parameters which have smallest precipitates and longest settling time. Evaluation liposome particle size and zeta potential were used DLS, morphology was used TEM, and entrapment efficiency was used dialysis. The most optimal formulation was tested their antiproliferative activity compared with not encapsulated extracts used 3-(4,5-dimethylazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. The result showed that there was antiproliferative activity in encapsulated extracts. IC50 encapsulated extracts was 45.762 μg/ml and IC50 extracts was 36.399 μg/ml. Liposome particle size was below 445 nm. Zeta potential was -7.51 mV. Morphology was LUV and MVV. Entrapment efficiency was 63.80%. It could be concluded that encapsulation of turmeric extract into liposome could reduce its toxicity against cancer cells.

Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg•mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene. Systémy transportu léčiv pomocí liposomů udržují velký potenciál pro léčbu rakoviny. Cílem této studie bylo navrhnout nanozařízení pro cílené ukotvení liposomů (s a bez cholesterolu) s enkapsulovanými protinádorovými léky a k povrchu připojeným antisense oligonukleotidem pro gen n-myc. K dosažení tohoto hlavního cíle byly připraveny liposomy s enkapsulovaným doxorubicinem, ellipticinem a etoposidem. Byly charakterizovány měřením intenzity fluorescence, zatímco účinnost enkapsulace byla odhadována na 16 %. Proces hybridizace jednotlivých oligonukleotidů tvořících nanokonstrukt byl sledován spektrofotometricky a elektrochemicky. Koncentrace ellipticinu, doxorubicinu a etoposidu připojených k nanokonstruktu v liposomech modifikovaných zlatými nanočásticemi byly 14, 5 a 2 µg•ml(-1). Autorům studie se podařilo prokázat, že liposomy jsou vhodné pro transport protinádorových léků a antisense oligonukleotidu, který může blokovat expresi n-myc genu.