1. An autonomously replicating eukaryotic expression vector with a tetracycline-responsive promoter
- Author
-
Jay M. Feingold and Zhihui Lang
- Subjects
DNA Replication ,Transcription, Genetic ,Operon ,Genetic Vectors ,Restriction Mapping ,Biology ,Kidney ,Cell Line ,chemistry.chemical_compound ,Transactivation ,Transcription (biology) ,Gene expression ,Chlorocebus aethiops ,Genetics ,Escherichia coli ,Animals ,TetR ,Cloning, Molecular ,Luciferases ,Promoter Regions, Genetic ,Gene ,Selectable marker ,Mammals ,General Medicine ,Transfection ,Tetracycline ,Recombinant Proteins ,Cell biology ,Eukaryotic Cells ,chemistry ,Gene Expression Regulation ,DNA Transposable Elements ,Plasmids - Abstract
The control elements of the tetracycline-resistance operon encoded in Tn 10 of Escherichia coli have previously been utilized by Gossen and Bujard [Proc. Natl. Acad. Sci. 89 (1992) 5547–;5551] to establish an efficient transcription regulatory system in mammalian cells. We describe here a modification of this vector system that encodes all required elements, including a neomycin-resistance gene, on an autonomously replicating episome. This, thereby, allows for improved transfection frequency and episome stability, providing an easily selectable marker gene and a promoter whose activity can be easily modulated to control gene expression.
- Published
- 1996