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Here is reported a microfluidic single-cell method for real-time monitoring of the effects of curcumin on the calcium change in a single glioma cell. The method is based on the quantitative measure...

Here, we report a microfluidic same-single-cell analysis to study the inhibition of multidrug resistance due to drug efflux on single leukemic cells. Drug efflux inhibition was investigated in the microfluidic chip using two different fluorescence detection systems, namely, a compact single-cell bioanalyzer and the conventional optical detection system constructed from an inverted microscope and a microphotometer. More importantly, a compact signal generator was used to conduct dielectrophoretic cell trapping together with the compact SCB. By using the DEP force, a single acute myeloid leukemia cell was trapped in the cell retention structure of the chip. This allowed us to detect dye accumulation in the MDR leukemic cells in the presence of cyclosporine A (CsA). CsA and rhodamine 123 were used as the P-glycoprotein inhibitor and fluorescent dye, respectively. The result showed that the Rh123 fluorescence signal in a single-cell increased dramatically over its same-cell control on both fluorescence detection systems due to the inhibition by CsA.

We report on conductometric study of complexation between benzylbisthiosemicarbazone [(2E,2′E)-2,2′-(1,2-diphenylethane-1,2-diylidene)bis(hydrazine-1-carbothioamide)] with Zn2+, Cr3+, Co2+, and Ni2+ cations at different temperatures in acetonitrile-dimethylformamide binary solvents of varied composition. The equilibrium constant and standard thermodynamic parameters (ΔcH0 and ΔcS0) of the complexes formation have been determined and found to be dependent on the binary solvent composition, the metal ion nature, and temperature.