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Background: Epidemiological studies have shown the prevention of cardiovascular diseases through the regular consumption of vegetables. Eruca sativa Mill., commonly known as rocket, is a leafy vegetable that has anti-inflammatory activity. However, its antiplatelet and antithrombotic activities have not been described. Methods: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL), was evaluated on human platelets: (i) P-selectin expression by flow cytometry, (ii) platelet aggregation induced by ADP, collagen and arachidonic acid, (iii) IL-1β, TGF-β1, CCL5 and thromboxane B2 release, and (iv) activation of NF-κB and PKA by western blot. Furthermore, (v) antithrombotic activity (200 mg/kg) and (vi) bleeding time in murine models were evaluated. Results: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL) inhibited P-selectin expression and platelet aggregation induced by ADP. The release of platelet inflammatory mediators (IL-1β, TGF-β1, CCL5 and thromboxane B2) induced by ADP was inhibited by Eruca sativa Mill. aqueous extract. Furthermore, Eruca sativa Mill. aqueous extract inhibited NF-κB activation. Finally, in murine models, Eruca sativa Mill. aqueous extract showed significant antithrombotic activity and a slight effect on bleeding time. Conclusion: Eruca sativa Mill. presents antiplatelet and antithrombotic activity.

Rocket (Eruca sativa Mill.) is a green leafy vegetable that is increasingly used as an ingredient in salad. The floating system is a way to grow this crop, at high density from sowing to harvest and easy to use in relation substrate culture, and it is well known water culture can be limited by hypoxia. Few studies have examined the use of peroxides or peracetic acid in nutrient solutions as a source of oxygenation. The aim of the study was to evaluate the rocket grown in substrates as compared to the floating system, with a stabilized mixture of peracetic acid in the nutrient solution. The experiment was carried out in and Talca (Chile) from October to November 2008. The treatments were substrate culture perlite and composted pine bark (1:1); floating system with trays filled with the same substrate and 0, 40 and 80 mg·L−1 of stabilized peracetic acid mix (PA). The fresh and dry weight of rocket leaves at harvest we evaluated. We recorded total nutrient solution uptake; the average dissolved oxygen; pH an...

Raw salad vegetables are typically consumed without being cooked. This study compared peracetic acid mix (PAA) and sodium hypochlorite (SH) as disinfectants on vegetables postharvest. Tomato, sweet pepper and cucumber were evaluated in three different experiments: (1) determination of organoleptic characteristics of vegetables by consumer preference; (2) disinfectant capacity comparison of PAA versus SH; and (3) measurement of phytotoxicity of disinfectant products, expressed as alteration of the surface of sweet peppers. Each treatment was replicated four times, and all procedures simulated the procedures carried out in industry. No differences in fruits washed with different treatments were found by the panel. Starting at Day 15, the peracetic acid mixture (PAA) showed better disinfection performance than sodium hypochlorite (SH). The results indicate that the peracetic acid mix is better for washing fruit and improving postharvest life as it is better for the environment (due to low toxicity) and for health safety and does not affect the taste characteristics of the fruit.

Cardiovascular disease prevention is of high priority in developed countries. Healthy eating habits including the regular intake of an antithrombotic diet (fruit and vegetables) may contribute to prevention. Platelet function is a critical factor in arterial thrombosis and the effect strawberries have is still unclear. Therefore, the aim of this study was to systematically examine the action of strawberries in preventing platelet activation and thrombus formation. Strawberry extract concentration-dependently (0.1–1 mg/ml) inhibited platelet aggregation induced by ADP and arachidonic acid. At the same concentrations as strawberry inhibits platelet aggregation, it significantly decreased sP-selectin, sCD40L, RANTES, and IL-1β levels. The strawberry may exert significant protective effects on thromboembolic-related disorders by inhibiting platelet aggregation. Also, this suggests that antithrombotic activity may have novel anti-inflammatory effects.

Nitrate accumulation in vegetable represents a serious threat to man's health. Different methods have been reported to reduce the nitrate accumulation in lettuce and other leafy vegetables with or without variation in the yield. The aim of this experiment was to show the different behavior of some cultivars of lettuce using the replacement of N‐NO3 for chloride in the nutrient solution during last week before harvest. The trial was carried out with three lettuce cultivars: Domino, Elvira, and Daguan and took place in southern Spain (Almeria) under greenhouse by NFT. The results indicated a correct method to decrease nitrate content in leafy vegetables and perhaps sulphate could be an anion to study their effect in this way.

Background: Regular consumption of fruits and vegetables (e.g., tomatoes) has been shown to be beneficial in terms of reducing the incidence of cardiovascular diseases. The industrial processing of tomatoes into tomato-based products includes several thermal treatments. Very little is known on the effect of tomato industrial processing on antiaggregatory activity and phenolic profile. Methods: It was assessed the effect of tomato and by-products extracts on platelet aggregation induced by ADP, collagen, TRAP-6 and arachidonic acid. These in vitro antithrombotic properties were further supported in an in vivo model of thrombosis. A set of antiplatelet compounds has been selected for HPLC analysis in the different extracts. Results: Some natural compounds such as chlorogenic, caffeic, ferulic and p-coumaric acids were identified by HPLC in tomatoes and its products may inhibit platelet activation. Red tomatoes, tomato products (sauce, ketchup and juice) and by-products extracts inhibited platelet aggregation induced adenosine 5'-diphosphate, collagen, thrombin receptor activator peptide-6 and arachidonic acid, but to a different extent. Also, pomace extract presents antithrombotic activity. Conclusions: Processed tomatoes may have a higher content of health-benefiting compounds than fresh ones. Pomace even presents the best antiplatelet activity. Finally, tomato products may be used as a functional ingredient adding antiplatelet activities to processed foods.

In seeking the functionality of foodstuff applicable to medicine, ripe tomato fruits were found to show an antiplatelet activity. Therefore, the bioactive compound was isolated, structurally identified, and studied for an inhibitory effects on platelet adhesion, secretion, and aggregation. The concentration of adenosine in ripe tomato fruits (pulp and skin extracts) and its processing by-products (paste and pomace) was determined by reversed-phase high-performance liquid chromatography (HPLC). According to platelet aggregation inhibition induced by ADP, the total extract residual was fractionated by liquid-liquid separation, obtaining aqueous, ethyl acetate and petroleum ether extracts. The aqueous extract was subjected to repeated permeation over sephadex LH-20 and semipreparative TLC. The isolate finally obtained was identified as adenosine on the basis of ESI-MS,1H NMR, HPLC, and UV spectra. Adenosine concentration dependently (2.3–457 μM) platelet aggregation inhibited induced by ADP. Also, adenosine present inhibition of platelet secretion and thrombus formation under flow conditions. The quantitative HPLC analysis revealed significant amounts of adenosine in ripe tomato fruits and its processing by-products. From these results, extracts/fractions of ripe tomato fruits and their processing by-products may be referred to as functional food and functional ingredients containing a compound that inhibits platelet function with a potent preventive effect on thrombus formation, as those that occur in stroke.