1. The maize rough dwarf virion
- Author
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Guido Boccardo, Robert G. Milne, and Enrico Luisoni
- Subjects
Antiserum ,Maize rough dwarf virus ,biology ,viruses ,RNA ,biology.organism_classification ,Precipitin ,Virology ,Molecular biology ,Virus ,RNA silencing ,chemistry.chemical_compound ,chemistry ,Antigen ,Sodium dodecyl sulfate - Abstract
A serological analysis was made on maize rough dwarf virus fractions by immune electron microscopy, gel diffusion and slide precipitin tests to investigate the reactions of both protein and RNA. Antisera were made against different virus fractions, and existing sera against rice black streaked dwarf and maize rough dwarf viruses were also tested. Some of the sera contained antibodies against viral dsRNA. The serological behavior of poly[I]:poly[C] was similar to but not identical with that of viral RNA. One antigenic group in smooth cores was identified, and an additional group was found in spiked cores, associated with B spikes. Attempts to obtain antibodies against the outer capsid were not successful. A soluble antigen was identified as viral RNA. Disrupted protein preparations containing sodium dodecyl sulfate reacted with normal sera as well as virus antisera.
- Published
- 1975
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