1. Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation
- Author
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Anaïs Le Mauff, Emiliane Taillebois, Olivier Plantard, Alison Cartereau, Cédric Neveu, Steeve H. Thany, Claude Rispe, Claude L. Charvet, Hamza Chouikh, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Université d'Orléans (UO)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Infectiologie et Santé Publique (UMR ISP), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Carnot France Futur Elevage 'Xenobio-Tick' and the 'Screen-Robot' project (APR IA Région Centre Val de Loire). http://www.francefuturelevage.com/fr/activites-de-r-d#projetsfinances, and Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
- Subjects
0301 basic medicine ,Insecticides ,Nicotine ,Nicotinic acetylcholine receptors ,[SDV]Life Sciences [q-bio] ,Ixodes ricinus ,030231 tropical medicine ,Receptors, Nicotinic ,Article ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Microtransplantation ,medicine ,Animals ,lcsh:RC109-216 ,Pharmacology (medical) ,Nicotinic Agonists ,Receptor ,Acetylcholine receptor ,Pharmacology ,Methyllycaconitine ,Ixodes ,Synganglion membrane ,Chemistry ,3. Good health ,Cell biology ,Nicotinic acetylcholine receptor ,030104 developmental biology ,Infectious Diseases ,Nicotinic agonist ,Female ,Parasitology ,Acetylcholine ,Tick ,medicine.drug - Abstract
Nicotinic acetylcholine receptors are an important class of excitatory receptors in the central nervous system of arthropods. In the ticks Ixodes ricinus, the functional and pharmacological properties of nicotinic receptors located in their neurons are still unknown. The objective of this study was to characterize the pharmacological properties of tick nicotinic receptors using membrane microtransplantation in Xenopus laevis oocytes and two-electrodes voltage clamp method. The membranes microtransplanted were extracted from the tick synganglion. We found that oocytes microtransplanted with tick synganglion membranes expressed nicotinic acetylcholine receptor subtypes which were activated by acetylcholine (1 mM) and nicotine (1 mM). Currents induced by pressure application of acetylcholine and nicotine were diminished by 10 nM α-bungarotoxin and methyllycaconitine, suggesting that they expressed two subtypes of nicotinic receptors, α-bungarotoxin-sensitive and -insensitive, respectively. In addition, we found that nicotine receptors expressed in the synganglion membranes were poorly sensitive to the neonicotinoid insecticides clothianidin (CLT), imidacloprid (IMI), acetamiprid (ACE) and thiamethoxam (TMX), in agreement with their lack of activity as acaricides. Interestingly, current amplitudes were strongly potentialized in the presence of 1 μM PNU-120596. CLT was more active as an agonist than IMI, TMX and ACE. Finally, we demonstrated that microtransplantation of purified membrane from the tick synganglion can be a valuable tool for the development and screening of compounds targeting tick nicotinic acetylcholine receptor subtypes., Graphical abstract Image 1
- Published
- 2020