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In the future, learning will be essentially characterized by the ability to regulate the learning process and monitor success independently from a teacher. The technical possibilities offer better access to learning contents, precise and more individualized feedback, and learning phases adapted precisely to the needs of the learner in terms of scope and pace. In this study, we investigate an important aspect of the digitization of teaching/learning processes using the example of laboratory scripts for chemistry students at university. The focus is on looking up terms and concepts in preparation for the lab internships, firstly in a paper-based glossary and secondly in a digital glossary. During a two-day study, a total of 16 students prepared for experiments on two topics with completely identical materials. We then studied the influence of content knowledge, motivation, and cognitive load. While all students show significant learning achievements, there are no significant differences between the groups. Furthermore, results show that pure digitization of information has no effect, despite the theoretically assumed advantages.

Science subjects at pre-tertiary and tertiary education levels are important for socio-economic and industrial development of any country; however, they are difficult for students to construct their concepts. In Tanzania, insufficient or lack of practical experiments are major challenges for science subjects due to insufficient or lack of laboratories, apparatus, expertise or reagents. Thus, this research assesses the effectiveness of paper-based against virtual laboratory experiences towards improvement of real (hands-on) chemistry practical in Tanzanian secondary schools focusing in Dodoma region. Chemistry virtual laboratory was developed and being deployed at Dodoma Secondary School for students who were never done practical sessions before to avoid biasness towards the study. The students were divided into three groups, namely paper-based and real laboratory as control groups (CG) and virtual laboratory as the experiment group (EG). Each group was further divided into two groups for the rest approaches forming six (6) groups. For EG, students were taught based on instructional approach which was enriched by computer animations in the computer laboratory. Results indicate that students who firstly attended virtual laboratory performed better in real laboratory than those who firstly attended real laboratory. Furthermore, the best progressive learning and performance for real experiments appears when the virtual laboratory preceded paper-based practical experiments. Thus, virtual laboratory is a very useful tool for learning chemistry practical not only to schools without laboratories but also to those with laboratories; and it should be considered by all the pre-tertiary schools in Tanzania and other schools in similar situations.

Although chemistry-focused socio-scientific issues support the 'relevance' model of chemistry education, the related literature has lacked any systematic review handling them together. For this reason, this research aimed to thematically synthesize the research papers on chemistry-focused socio scientific issues (SSI) from 2008 to 2020 and inferentially evaluate them in terms of the relevance model of chemistry education. After searching international and national well-known databases through relevant keyword patterns (e.g., Pattern 1: socio-scientific issues and chemistry education), 65 research papers were apparent for the systematic review. Then, the authors generated primary and secondary codes for the research papers and then inferentially marked their 'relevance' components. The systematic review indicated variation of research areas (e.g., relevance model of chemistry education) and dominant research foci for different themes (e.g., competencies and related variables for the theme 'aims'; pollution, energy, industry and fabrication-based problems for the theme 'SSI'; organic compounds for the theme 'chemistry concepts'). Further, it revealed that the research papers on chemistry-focused SSI had some shortcomings at handling all components of the relevance model in a balanced way. The current research suggests professionally training teachers about how to integrate chemistry-focused SSI and the relevance model into school chemistry.

Gender inequality is strongly linked with poor performance in science and mathematics for female students in secondary schools owing to socio-economic and cultural issues as well as learning strategies. According to the Certificate of Secondary Education Examination results of 2021 in Tanzania, the failure rate for female students was higher than that for male students, and such failure in chemistry was associated with inadequate knowledge about the tested concepts, failure to identify the requirements of the respective questions and lack of adequate mathematical skills. Real practical sessions are integral parts of chemistry to enhance the learning environments for both female and male students. However, the real laboratory practices especially for chemistry are challenged with the costs of the construction of physical laboratory infrastructure and reagents, and the time-consuming and physical demands on personnel during real practical sessions. Thus, this study explores gender-based effects of the chemistry virtual laboratory against a paper-based approach towards a real chemistry practical in Tanzanian secondary schools. The results indicate that the combined exposures of virtual laboratory and paper-based practicals improve real practical performance for both female and male students. Moreover, the performance of real practical practices progressively improved for female students when they were first exposed to the virtual laboratory and then the paper-based practical. Such improvements can be associated with the virtual laboratory providing interactive learning environments which support female students to connect and engage.

This study presents an innovative lateral flow microfluidic paper-based analytical device (μPAD) designed for conducting quantitative paper-based enzyme-linked immunosorbent assays (p-ELISA), seamlessly executing conventional ELISA steps in a paper-based format. The p-ELISA device utilizes a passive fluidic circuit with functional elements such as a multi-bi-material cantilever (B-MaC) assembly, delay channels, and a buffer zone, all enclosed within housing for autonomous, sequential loading of critical reagents onto the detection zone. This novel approach not only demonstrates a rapid assay completion time of under 30 min, but also boasts reduced reagent requirements, minimal equipment needs, and broad applicability across clinical diagnostics and environmental surveillance. Through detailed descriptions of the design, materials, and fabrication methods for the multi-directional flow assay (MDFA), this manuscript highlights the device’s potential for complex biochemical analyses in a user-friendly and versatile format. Analytical performance evaluation, including a limit of detection (LOD) of 8.4 pM for Rabbit IgG, benchmarks the device’s efficacy compared to existing p-ELISA methodologies. This pioneering work lays the groundwork for future advancements in autonomous diagnostics, aiming to enhance global health outcomes through accessible and reliable testing solutions.

Fiber-reinforced polymer composites (FRPCs) present a promising solution for diverting waste generated by the pulp and paper industry from landfills. The global pulp and paper industry generated 110 million tons [...]

Paper and board are widely used as food contact materials. For such sensitive applications, consumer safety regarding the transfer of chemical components and contaminants to the food needs to be established. Such safety assessments are becoming increasingly challenging not only due to intentionally added substances but also non-intentionally added substances. In the European Union, compliance testing and safety evaluation of paper in food contact are largely based on national legislation and standards. The underlying tests are conventional methods, often overestimating and sometimes underestimating the migration into food. In this article, the relevant standard test methods are contrasted with currently available scientific knowledge. The scientific approaches to develop and identify suitable test methods are critically reviewed. Furthermore, theoretical predictions via mathematical modeling, with the aim to realistically simulate transfer to food, are presented and discussed in comparison with available migration studies with foods. Objectives are to (i) summarize the actual scientific knowledge in the field and draw conclusions regarding the potential and limitations of the existing test methods and (ii) identify research gaps toward a better qualitative and quantitative understanding of transport processes of volatile and non-volatile substances from paper and board into foods.

Pulp and paper manufacturing ranks as one of the most water and energy-intensive sectors globally, invariably leading to significant environmental pollution e.g. issues related to air pollution and waste disposal. Also, as global competition intensifies, prices of forest products are expected to keep decreasing. To ensure their viability, traditional producers must augment their revenue streams by diversifying into the production of bioenergy and biomaterials, alongside traditional wood, pulp, and paper products. A feasible solution is the integrated products biorefineries which provide a distinctive chance to pulp and paper industry to enhance revenues as well as reduce their environmental impact. Since pulp and paper stands as the predominant industry in Sweden, this review article explores the emerging biorefinery process options and main technological pathways being developed within Swedish pulp and paper mills. It also delves into the significant challenges that are being encountered in this evolving landscape and what possibilities lie ahead for responsible and sustainable pulp and paper operations.

The fraudulent practice of adding ammonium salts to milk in order to enhance the nitrogen content has serious health implications including renal failure. Ammonium ion detection has been targeted through the conventional analytical methods however, lack of portability and complex operation limits their use for field applications. In this scenario, paper-based test strips and electrochemical sensors can provide strategies for point-of-test applications. Here, an ion-selective paper-based chromogenic strip and an electrochemical sensor has been developed for detection and quantification of ammonium ions. The developed paper-based chromogenic strip showed detection limit of 0.008 % (w/v) for ammonium ions, whereas, electrochemical sensor showed an LOD of 0.0062 ± 0.0023 % (w/v) with a sensitivity of 1.6 × 10−5 A.% −1.mm−2. The developed paper-based chromogenic strip and electrochemical sensor can be employed by regulatory bodies and dairy industries to ensure the safety and quality of milk.

An inexpensive at-home quantitative analysis activity was developed for the determination of glucose in unknown samples using paper-based microfluidic devices. All of the materials and reagents for the activity fit in a small kit that was mailed to students. The only items students needed to supply were water and a smartphone. Microgram quantities of glucose were dried down in microcentrifuge tubes and included in the kit so that students could prepare external standards. Student results distinguished between two different concentrations of glucose that served as unknown samples. The combination of paper-based devices and dried-down microgram quantities of reagents provides a foundation for the development of other at-home or in-person experiments.

We describe a paper-based microfluidic device for iron(III) assay in an applied instrumental analysis laboratory. Simple and convenient devices were fabricated by the students using patterning of filter paper to investigate three different procedures: drawing a boundary with eyeliner or wax pen; drawing a boundary with waterproof permanent marker pen; or drawing no boundary. These test papers were used for the analysis of iron(III) in water samples by smartphone-based colorimetric detection with thiocyanate. Additionally, the interference effect of other metal cations was done to further demonstrate practical utility. The results for field sample detection using this microdevice were satisfactory and show promise for use in other applications.

The use of synthetic chemicals in acid‒base testing has raised concerns about their environmental impact. The use of natural materials to produce natural indicator paper is an attempt to reduce the negative impact on the environment. Binahong (Anredera cordifolia) seeds can replace conventional chemical synthetic materials. This study aims to develop Binahong seed indicator paper as an alternative environmentally friendly material for acid‒base testing. The method used in this research is experimental and involves the extraction of Binahong seeds, making indicator paper with a certain formulation, and testing the performance of the indicator paper using standard acid and base solutions. Data collection was performed via the observation and measurement of certain parameters. The test results of the Binahong natural indicator paper revealed a significant color change when 95% ethanol was used, which produces a red or purple color at pH 1‒7, a blue color at pH 8‒10 and a yellow color at pH 11‒14. Binahong seeds have potential as an environmentally friendly alternative in acid‒base testing because they respond well to variations in the acidity and basicity of the solution.

The sustainable use of natural resources is becoming an increasingly important issue today. Stone paper, produced as an alternative to cellulose-based paper from the forest, is rich in minerals and produced without cellulose and water. This study focuses on the behavior of screen-printing ink on two different papers, stone paper and coated sticker paper. Properties such as ink adhesion, rubbing resistance, optical printing ink density, ink consumption, and lightfastness were measured on these surfaces. Solvent- and UV-based inks were used, and printing was carried out on cellulose-based (coated sticker paper) and mineral-based (stone paper) paper layers using three different mesh counts (90, 120, and 140 tpc). The rubbing resistance and lightfastness of the papers were also measured. The present findings revealed that stone paper had the same printability properties as cellulose-based paper. The study concluded that using a 140 tpc mesh with both types of ink results in a high-lightfastness ink layer and lower ink consumption. UV-based inks exhibited high rub resistance across all mesh counts. Additionally, when printing with stone paper, there will be a reduction in ink consumption, thereby achieving cost savings. Based on the present findings, it was concluded that water- and oil-resistant stone paper can be considered an essential alternative in many fields, including the printing industry.

The majority of chemical experiments conducted during educational programs are carried out in laboratories because they require instructors with special skills, in addition to large, expensive instruments. Recently, there has been an increasing demand for chemical experiments that can be carried out anywhere. Herein, we propose a novel type of paper-based analytical device (PAD) for enabling quantitative analysis without the requirement for a micropipette, since the PAD features a large sample loading zone and a waste zone, enabling accurate volume control of a liquid sample. We initially employed a micropipette to evaluate the PAD and demonstrate the quantitative analysis of ascorbic acid (AA) and pH using different loading volumes. Finally, we determined the AA concentrations and pH values of commercially available beverages using disposable plastic droppers, and the obtained results were in good agreement with those obtained through conventional methods. This PAD format can therefore be used as a novel educational tool for conducting certain chemical analyses in remote learning environments.

This work presents a dynamic modeling approach for analyzing the behavior of a bi-material cantilever actuator structure, consisting of a strip of filter paper bonded to a strip of tape. The actuator’s response is induced by a mismatch strain generated upon wetting, leading to the bending of the cantilever. The study delves into a comprehensive exploration of the dynamic deflection characteristics of the bilayer structure. It untangles the intricate connections among the saturation, modulus, hygro-expansion strain, and deflection, while uniquely addressing the challenges stemming from fluid–structure coupling. To solve the coupled fluid–solid differential equations, a combined numerical method is employed. This involves the application of the Highly Simplified Marker and Cell (HSMAC) technique for fluid flow analysis and the Finite Difference Method (FDM) for response deflection computation. In terms of the capillary flow model, the Computational Fluid Dynamics (CFD) simulations closely align with the classical Washburn relationship, depicting the wetted front’s evolution over time. Furthermore, the numerical findings demonstrate that heightened saturation levels trigger an increase in hygro-expansion strain, consequently leading to a rapid rise in response deflection until a static equilibrium is achieved. This phenomenon underscores the pivotal interplay among saturation, hygro-expansion strain, and deflection within the system. Additionally, the actuator’s response sensitivity to material characteristics is highlighted. As the mismatch strain evolving from paper hygro-expansion diminishes, a corresponding reduction in the axial strain causes a decrease in response deflection. The dynamic parameter demonstrates that the deflection response of the bilayer actuator diminishes as dynamic pressure decreases, reaching a minimal level beyond which further changes are negligible. This intricate correlation underscores the device’s responsiveness to specific material traits, offering prospects for precise behavior tuning. The dependence of paper modulus on saturation levels is revealed to significantly influence bilayer actuator deflection. With higher saturation content, the modulus decreases, resulting in amplified deflection. Finally, strong concordance is observed among the present fluidically coupled model, the static model, and empirical data—a testament to the accuracy of the numerical formulation and results presented in this study.

A new method was proposed for point-of-care testing (POCT) of immunosuppressants in blood using thin-layer paper spray ionization and a miniature mass spectrometer. The aluminum thin-layer plate was used as paper spray substrate, and cut into an equilateral triangle. Aliquots of 10 μL blood sample were loaded on the aluminum thin-layer plate, and then 20 μL methanol was dropped as elution/spray solvent. The tip of the triangular plate was placed 1.0 cm in front of the discontinuous atmospheric pressure interface (DAPI) of the miniature mass spectrometer. When the spray voltage was applied, the sample was eluted from the triangular plate and formed paper spray ionization at the tip. Coupled with the miniature mass spectrometer, five immunosuppressants were detected under negative ion mode. The precursor ions ［M-H］- of tacrolimus, sirolimus, everolimus, ciclosporin A, and mycophenolic acid are m/z 802.6, 912.6, 957.1, 1 200.8, 319.1, and product ions are m/z 560.2 and 604.3, m/z 590.3 and 321.2, m/z 590.3 and 365.2, m/z 1 088.8, m/z 287.4 and 275.1, respectively. Based on the observed signal-to-noise ratio (S/N) of the most intense tandem mass spectrometry (MS/MS) fragment peaks and using the S/N=3 and 10 as the criteria, the limits of detection (LODs) and quantitation (LOQs) are estimated to be 0.5-5 mg/L and 1-10 mg/L, respectively. The internal standard method was used for quantitative analysis, and indapamide was used as the internal standard compound. Sufficient linearity for each analyte is obtained with the correlation coefficients (r) greater than 0.99. The proposed method displays good recoveries in the range of 91.0%-114.3% with the relative standard deviations (RSDs) less than 8.4%. Moreover, the developed method was used for the analysis of blood samples, which spiked with tacrolimus, sirolimus, everolimus, cyclosporine A and mycophenolic acid at the concentration of 100 mg/L. The analytical results demonstrated the consistency between the proposed method and the traditional liquid chromatography-mass spectrometry (LC-MS) method. The entire analysis procedure can be completed within 1 min. The proposed method is simple, fast, accurate, efficient, and suitable for POCT of immunosuppressants in blood.

The Making Introductory Courses Real while Online (MICRO) laboratory project was developed to meet the need for hands-on experiments, focused on topics in analytical chemistry, to be delivered safely remotely or in a socially distanced in-person lab. Unlike more traditional lab experiments, MICRO laboratories use only microgram or nanogram amounts of chemicals; paper microfluidic technology is used to store and mix reactants. Instructional materials use an inquiry-based approach and are situated in a context that highlights the human impacts of the scientific analysis. To support broader-scale implementation of the experiments and promote a shift to more inquiry-based laboratory instruction, an array of supports were developed, including adaptable instructional materials, instructional videos for lab preparation, resource guides, and an introductory workshop. A cohort of nine institutions implemented MICRO laboratories both remotely and in person during Fall 2020. Students were able to successfully complete the experiments, and the inquiry nature of the laboratories led to an increased comfort with the trial-and-error nature of authentic scientific practice. Additionally, most faculty participants indicated a commitment to an increased degree of inquiry in their laboratory pedagogy.

Compared to traditional lithium metal batteries, anode-free lithium metal batteries use bare current collectors as an anode instead of Li metal, making them highly promising for mass production and achieving high-energy density. The current collector, as the sole component of the anode, is crucial in lithium deposition-stripping behavior and greatly impacts the rate of Li depletion from the cathode. In this study, to investigate the lithiophilicity effect of the current collector on the solid electrolyte interface (SEI) film construction and cycling performance of anode-free lithium batteries, various lightweight paper-based current collectors were prepared by electroless plating Cu and lipophilic Ag on low-dust paper (LDP). The areal densities of the as-prepared LDP@Cu, LDP@Cu-Ag, and LDP@Ag were approximately 0.33 mg cm−2. The use of lipophilic Ag-coated collectors with varying loadings allowed for the regulation of lipophilicity. The impacts of these collectors on the distribution of SEI components and Li depletion rate in common electrolytes were investigated. The findings suggest that higher loadings of lipophilic materials, such as Ag, on the current collector increase its lipophilicity but also lead to significant Li depletion during the cycling process in full-cell anode-free Li metal batteries. Thus, moderately lithiophilic current collectors, such as LDP@Cu-Ag, show more potential for Li deposition and striping and stable SEI with a low speed of Li depletion.

This study investigates the physical, mechanical, and structural characteristics of handmade paper samples derived from cellulose extracted from grass clippings using two distinct methods as follows: (1) alkali treatment and (2) alkali treatment followed by bleaching, coupled with the incorporation of barium sulfate as a mineral filler. Our investigation revealed that the handmade paper samples’ densities, moisture contents, and thicknesses varied within the ranges of 0.436 to 0.549 g/cm3, 5.60 to 2.51%, and 0.41 to 0.50 mm, respectively. The tensile strength and folding endurance of the papers produced through alkali treatment with barium sulfate were notably superior to those produced from bleached pulp and barium sulfate. Our analysis indicates that several critical factors, including paper density, thickness, the crystallinity index, and the microfibrillar structure of cellulose, intricately influence the mechanical and strength properties of the samples. Using Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) techniques, we identified characteristic cellulose bonds and examined cellulose’s crystalline and amorphous phases. Additionally, the crystallinity index of the samples was determined using both the Segal and peak deconvolution methods. Scanning electron microscopy (SEM) micrographs revealed interconnected networks of cellulose fibers with varying thicknesses and lengths, along with incorporated mineral filler within the cellulose fiber structure. Variations in mineral particle retention were attributed to the presence or absence of cellulose microfibrils. These findings contribute to our understanding of the observed strength characteristics of the paper samples and underscore the potential applications of cellulose derived from grass clippings, especially when combined with barium sulfate as a mineral filler in paper production.

The research results and evaluation of the applicability of the original composition of hybrid paper based on silver nanowires (AgNWs), cellulose pulp (CP), and carbon nanotubes (CNTs) are presented and discussed. The material tested was used to manufacture sensors for mechanical deformation resulting from external influences or related to human activity interactions. The sensors were fabricated using an AgNWs + CP suspension and additives by the vacuum filtration method. The substrate obtained was machined and then laminated with a layer of poly(dimethylsiloxane) (PDMS). The recorded responses to selected types of imposed mechanical interactions in the form of changes in the relative resistance of the sensor throughout the tests showed a close cause-and-effect relationship. The response of the tested systems when applying an alternating magnetic field was also observed. The results indicate that the proposed solutions can find application in the monitoring of mechanical interactions resulting from the dynamic behavior of physical objects, as well as derived from selected human vital functions.

Carbon fiber paper is one of the most important substrates used in the gas diffusion layer of the polymer electrolyte membrane fuel cell. A novel approach to creating carbon fiber paper using polyacrylonitrile compounds was proposed in this study. The technique incorporated varying amounts of carbon materials, including carbon black (Vulcan), graphite powder, and carbon nanotubes. Polyacrylonitrile carbon materials were prepared through the spinning process, followed by oxidative stabilization under an oxygen atmosphere at a temperature range of 160–300 °C as the first step. The pliable material undergoes a conversion process to create a compound or ladder that is non-plastic, which is then stabilized in an N2 atmosphere. The fibers are then pre-carbonized at temperatures ranging from 300 to 600 °C and further carbonized between 600 and 1100 °C. After that, Teflon is added and the resulting fibers are made into sheets during the production process. Finally, physical measurements and electrochemical methods such as: checking the amount of water absorption, scanning electron microscope (SEM), chronopotentiometry, electrochemical impedance spectroscopy, electron resistance of carbon sheet, double layer capacitance, and surface roughness parameter were evaluated. The results indicate that the best performance is related to CP7 carbon paper (with a 30–70 % combination of polyacrylonitrile and carbon black).

We present a facile one-step hydrothermal method for the fabrication of free-standing graphene paper using aqueous graphene oxide (GO) dispersion as the starting material. Notably, the method does not require the use of any additives for the reduction and preparation of the graphene paper. The resulting graphene paper exhibits a high degree of reduction, as confirmed by X-ray photoelectron spectroscopy (XPS) analysis, revealing a carbon-to-oxygen (C/O) ratio of ∼7. The fabricated graphene paper also demonstrates excellent conductivity, with a measured value of ∼1900 S/m, and sheet resistance of around 100 Ω/sq. Furthermore, the energy storage capability of the graphene paper based electrode is evaluated, which shows a promising specific capacity of 249 F/g at a current density of 1 A/g in 1M H2SO4 as the ionic medium. Additionally, the electrode has also demonstrated remarkable energy density and power density values of 28.68 Wh/Kg and 455 W/Kg, respectively. Overall, the resulting graphene paper exhibits high conductivity and excellent electrochemical performance, making it a promising candidate for various energy storage applications.

The development of a simple yet greener microfluidic paper-based analytical device (μ-PAD) for on-site detection of Mn(II) in various types of waters using tannin as a natural reagent was described. The μ-PAD consists of twelve detection zones, created on a Whatman Number 1 filter paper by a simple drawing technique using an acrylic watercolor. The detection of Mn(II) was based on the color change on the reaction zone due to the reaction between Mn(II) and the pre-deposited tannin. The μ-PAD image was captured by a portable smartphone detector, and the blue intensity was digitized using a color picker application to generate the reflectance as the analytical response. The proposed method was characterized by a linear dynamic range of 0.05–0.25 mg L−1 with the limit of detection (LOD) for the determination of Mn(II) of 0.026 mg L−1. The other analytical merits of the proposed method, such as precision (RSD, 1.107%), accuracy (E, 6.697%), and recovery (104–112%), were all comparable to the existing spectrophotometric methods. The method’s successful application to natural water samples from manganese mining sites aligns with the reference spectrophotometric method, indicating its good selectivity and accuracy without significant influence of commonly associated interfering ions.

This communication describes a method to detect aluminum in gastric drugs by the naked eye using a microfluidic paper-based analytical device ([mu]PAD). Aluminum ions react with chrome azurol S (CAS) and cetyltrimethylammonium bromide (CTAB) to form a colored complex, which generates a colored band on the [mu]PAD. A printed ruler can be used to measure the length of the colored band on an individual paper channel for the quantification of an aluminum ion. The [mu]PAD system was designed for use in a modern instrumental analysis course taken by students majoring in Food Quality and Safety. This analytical protocol could also be modified to be used as a demonstration or laboratory experiment in a high school chemistry course with some simplifications to the procedures.

This technology report outlines the design and construction of a simple Arduino-based pH sensor using universal pH paper; it can be assembled for less than $40 and is ideal for use in laboratory classrooms teaching students with visual disabilities. The device is simple to construct and suitable for adoption in school chemistry laboratories because of its miniature size, user-friendliness, and flexible coding. The Arduino-based pH sensor allows the visually impaired to use and interpret universal pH paper in measuring pH, which could not be previously attempted. A change in color of the indicator paper, as detected by the RGB color sensor, generates a distinctive audible tone that corresponds to the pH value being measured. This device can function as a helping hand in the learning process by facilitating a more exciting and meaningful laboratory exercise experience not only for the visually impaired but also for their normal-sighted peers.

The development of simple and accurate detection of uracil-DNA glycosylase (UDG) is of great significance for early clinical diagnosis and biomedical research. Here, we on the first effort introduced the uracil bases into the rolling circle amplification (RCA) reaction to produce the functionalized pure DNA hydrogel (PDH) for UDG detection. During RCA process, methylene blue (MB) molecules as the indicators were encapsulated into PDH. The addition of UDG can remove the uracil bases of PDH to generate abasic sites, which are further cleaved with the assistance of apurinic/apyrimidinic endonuclease (APE), thus resulting in the dissociation of PDH to release blue MB. By combining with the paper analytical devices as the signal readout platform, a colorimetric and electrochemical dual-signal biosensor was constructed for convenient and accurate detection of UDG. The proposed MB@PDH-based dual-signal sensing system exhibited good selectivity and high sensitivity with a detection limit of 6.4 × 10−4 U/mL (electrochemical method). It was also demonstrated that this sensing system showed excellent performance in UDG inhibitor screening, thus providing great potential in UDG-related disease diagnosis and drug discovery.

This work reports the first use of rubber latex waste as the hydrophobic reagent for a low-cost, simple, and rapid fabrication of a paper towel-based experimental device (PTED) with a circular design of 5 mm to demonstrate the factors affecting chemical equilibrium; this serves as a user-friendly means for a laboratory experiment. The illustration of the factors affecting chemical equilibrium is implemented in the PTED. In this approach, the color change of a cobalt(II) complex reaction from pink to blue was observed by the naked eye on the PTED when a tiny drop of hydrochloric acid solution and the treatment of heat by using a hot air dryer (1200 W, 2 min) were applied to the PTED with the precoated cobalt solution (pink color), separately or together. The return of the color of the reactions from blue to pink was carried out by using a tiny drop of water and the treatment of cooling by using an ice bath for 10 min. In addition, the chemical equilibrium constant ("K"[subscript c]) can be easily quantified by processing digital images of the detection zones of PTED captured with a smartphone camera under a control light box at room temperature (˜25 °C), while image analysis was performed using ImageJ software. This PTED laboratory is a new approach not only to enhance students' learning of the factors affecting chemical equilibrium based on Le Châtelier's principle, but also to present potential applications of PTED into all levels of chemistry from middle school through college classes.

A two to three period analytical chemistry experiment has been developed which allows second year students to explore chemical color tests used to detect adulterated pharmaceuticals. Students prepare several paper analytical devices (PADs) to generate positive and negative controls antibiotics, along with cutting agents such as starch and chalk. These PADs are used to identify the active ingredients and excipients in mystery tablets prepared by their classmates. In the second part of the lab, the students select an individual color test and design an experiment to quantify their mystery pill's active pharmaceutical ingredient (API). Nearly all of the student groups were able to successfully identify adulterants present in their mystery tablets. The quantification of the mystery tablets was also successful with all but one group calculating the correct concentration within 6%. In a postlab assessment, the students identified their largest gains in their ability to analyze data and other information, skill in science writing, and learning of laboratory techniques.

This paper addresses the challenges of data sparsity and personalization limitations inherent in current recommendation systems when processing extensive academic paper datasets. To overcome these issues, the present work introduces an innovative recommendation model that integrates the wealth of structured information from knowledge graphs and refines the amalgamation of temporal and relational data. By applying attention mechanisms and neural network technologies, the model thoroughly explores the text characteristics of papers and the evolving patterns of user behaviors. Additionally, the model elevates the accuracy and personalization of recommendations by meticulously examining citation patterns among papers and the networks of author collaboration. The experimental findings show that the present model surpasses baseline models on all evaluation metrics, thereby enhancing the precision and personalization of academic paper recommendations.

Environmental pollution is a significant problem and is increasing gradually as more and more harmful pollutants are being released into water bodies and the environment. Water pollutants are dangerous and pose a threat to all living organisms and the ecosystem. Paper waste is one of the most widespread and largest wastes in the world. This research aims to address two important problems simultaneously: the reduction in solid waste in the environment using activated carbon from paper waste as potential adsorbents and the removal of harmful contaminants from water. Carbon from paper waste was activated with H2O2, HNO3, and KMnO4 for the adsorption of Cr(VI) and MB. SEM, EDX, FTIR, Raman, and BET were used to determine the properties of the materials. The surface morphology of the materials consisted of amorphous particles. EDX shows that all activated samples have a higher content of (O) than carbon paper waste. Adsorption studies showed that there was a stronger interaction between the pollutants and the adsorbent at a higher initial concentration (200 mg/L) than at the lower initial concentrations. The contact time data show that uptake increases when the interaction time between the contaminant and the adsorbent is increased. The tests for the pH of the solution show that the adsorption of Cr(VI) decreases when the pH is gradually increased, whereas the adsorption of MB increases when the pH of the solution is increased. The results fit better with the Freundlich isotherm and PSO models. The temperature studies show that the enthalpy was positive, indicating that the uptake process is endothermic. The Gibbs free energy values were all negative, indicating that adsorption between the adsorbents and the pollutants was favored. After four consecutive cycles, all the samples retained more than 60% of their uptake capability.

Pulp Injection Molding (PIM) enables manufacturers to produce unique and intricate geometries typically reserved for plastics but using paper. This innovation provides new options for brand owners, enhancing sustainability and [...]

Abstract Adverse Outcome Pathways (AOPs) have been proposed to facilitate mechanistic understanding of interactions of chemicals/materials with biological systems. Each AOP starts with a molecular initiating event (MIE) and possibly ends with adverse outcome(s) (AOs) via a series of key events (KEs). So far, the interaction of engineered nanomaterials (ENMs) with biomolecules, biomembranes, cells, and biological structures, in general, is not yet fully elucidated. There is also a huge lack of information on which AOPs are ENMs-relevant or -specific, despite numerous published data on toxicological endpoints they trigger, such as oxidative stress and inflammation. We propose to integrate related data and knowledge recently collected. Our approach combines the annotation of nanomaterials and their MIEs with ontology annotation to demonstrate how we can then query AOPs and biological pathway information for these materials. We conclude that a FAIR (Findable, Accessible, Interoperable, Reusable) representation of the ENM-MIE knowledge simplifies integration with other knowledge. Scientific contribution This study introduces a new database linking nanomaterial stressors to the first known MIE or KE. Second, it presents a reproducible workflow to analyze and summarize this knowledge. Third, this work extends the use of semantic web technologies to the field of nanoinformatics and nanosafety.

Buckypaper (BP), a flexible and porous material, exhibits photovoltaic properties when exposed to light. In this study, we employed radio frequency (RF) sputtering of zinc oxide (ZnO) followed by rapid thermal annealing to enhance the photovoltaic response of BP. We investigated the impact of various sputtering parameters, such as the gas flow ratio of argon to oxygen and deposition time, on the morphology, composition, resistivity, and photovoltaic characteristics of ZnO-modified BP. Additionally, the photovoltaic performance of the samples under different illumination modes and wavelengths was compared. It was found that optimal sputtering conditions—argon to oxygen flow ratio of 1:2, deposition time of 20 min, and power of 100 watts—resulted in a ZnO film thickness of approximately 45 nanometers. After annealing at 400 °C for 10 min, the ZnO-modified BP demonstrated a significant increase in photocurrent and photovoltage, along with a reduction in resistivity, compared to unmodified BP. Moreover, under gradient illumination, the ZnO-modified BP exhibited a photovoltage enhancement of 14.70-fold and a photocurrent increase of 13.86-fold, compared to uniform illumination. Under blue light, it showed a higher photovoltaic response than under other colors. The enhancement in photovoltaic response is attributed to the formation of a Schottky junction between ZnO and BP, an increased carrier concentration gradient, and an expanded light absorption spectrum. Our results validate that ZnO sputtering followed by annealing is an effective method for modifying BP for photovoltaic applications such as solar cells and photodetectors.

The dominance of plastics in the packaging market is due to their low weight and thickness, which save transportation costs. However, their non-biodegradability poses a significant threat to the environment. Paper, on the other hand, is considered as a safer alternative due to its natural composition and biodegradability. The porous structure of paper limits its application in packaging, and its poor water resistance further restricts its use in humid environments. Therefore, lamination is a method useful tool to improve the barrier properties of paper. Additionally, the researchers are focusing on developing biodegradable and water-based coatings with anti-fat properties as a green alternative to plastic packaging. The impact of a new grease-resistant coating composed of starch, gelatin and sodium alginate on the mechanical properties of paper was investigated through tensile, tearing, and bursting strength tests. The results showed significant improvements in the mechanical properties of the coated paper sheets. Furthermore, the biodegradability test indicated that the paper samples coated with the new composition showed a 50% weight loss after one week of incubation in the soil, and after three weeks, they exhibited 100% weight loss, demonstrating their outstanding biodegradability.

Students often ask about the practical application of concepts and knowledge gained from their coursework. Practicum worksheets provide an opportunity for students to engage in hands-on learning, identify their strengths, learn from their mistakes, and develop enhanced competence as learners. This study aims to develop an effective and valid worksheet on PjBL in whey kefir paper soap. Research and Development (R&D) is used as a method. Worksheet designs were produced using three formulas depending on production techniques. Based on Indonesia National Standard, Formula 1 (8% whey kefir) demonstrated the best qualities of the paper soap. The expert validation test of the worksheets showed a rcount value of 0.84 (valid). The percentage of worksheet answers was very good (88%). Student worksheet feasibility test results showed a rcount value of 0.84 (feasible). Practicum worksheets are categorized as very suitable for use as a learning media in an organic chemistry course.

Objectives: This study aims to determine the temporal stability of Schistosoma mansoni circulating cathodic antigens (CCA) in filter paper-based dried urine spot (FP-DUS) samples under varying temperatures condition. Methods: Urine from 20 children confirmed to have S. mansoni infection using Kato-Katz (at least 1 egg per gram of stool) and Schisto POC-CCA (2+ and 3+) methods were stored in form of FP-DUS and urine at room temperature (RT), 4 °C and −20 °C. Standard urine and FP-DUS Schisto POC-CCA methods were employed to detect CCA in urine and FP-DUS samples respectively, at weeks 4, 8 and 12. The results were reported as negative or positive (trace, 1+. 2+, and 3+). Results: In FP-DUS samples, POC-CCA scores initially increase after 4–8 weeks, but then showed a decrease in intensity while still remaining positive, independent of temperature condition. From week 4 to week 12, at least 80 % of urine samples had POC-CCA score of 3+, independent of temperature condition. However, 2 urine samples at RT tested negative at weeks 8 and 12. Conclusions: Despite the decrease in the intensity of test line in many samples, S. mansoni CCA remains stable and detectable in urine samples stored in FP-DUS.

Since the ban on single-use plastic articles in Europe, the food contact material (FCM) industry has been forced to move to more sustainable alternatives. Paper and board FCM are convenient alternatives but must be safe for consumers. This study aims to investigate potential migrations of various substances (e.g., plasticizers, photoinitiators, primary aromatic amines, mineral oil, and bisphenols) from straws and takeaway articles made of paper and board. Twenty straws and fifty-eight takeaway articles were carefully selected and investigated using liquid and gas chromatography coupled with tandem mass spectrometry or flame ionization detector. Fourteen substances of all the targeted categories were found in takeaway articles, including seven plasticizers, two photoinitiators, one primary aromatic amine, two bisphenols, and the saturated and aromatic fraction of mineral oil (MOSH and MOAH, respectively). In straws, fewer substances were detected, i.e., six substances, including three plasticizers, one photoinitiator, MOSH, and MOAH. At least one of the target substances was detected in 88% of the samples, demonstrating the importance of further evaluation of these materials. Finally, the associated risks were assessed, highlighting the potential risks for several types of articles regarding bisphenol A, one primary aromatic amine (3.3-DMB), and MOSH and MOAH.

This work focuses on a systematic method to produce Ag, Cu, and Ag/Cu metallic nanoparticles (MNPs) in situ assisted with ultrasound on cellulose paper. By tuning the concentration of AgNO3 and CuSO4 salt precursors and ultrasound time, combined with a fixed concentration of ascorbic acid (AA) as a reducing agent, it was possible to control the size, morphology, and polydispersity of the resulting MNPs on cellulose papers. Notably, high yield and low polydispersity of MNPs and bimetallic nanoparticles are achieved by increasing the sonication time on paper samples pre-treated with salt precursors before reduction with AA. Moreover, mechanical analysis on paper samples presenting well-dispersed and distributed MNPs showed slightly decreasing values of Young's modulus compared to neat papers. The strain at break is substantially improved in papers containing solely Ag or Cu MNPs. The latter suggests that the elastic/plastic transition and deformation of papers are tuned by cellulose and MNPs interfacial interaction, as indicated by mechanical analysis. The proposed method provides insights into each factor affecting the sonochemistry in situ synthesis of MNPs on cellulose papers. In addition, it offers a straightforward alternative to scale up the production of MNPs on paper, ensuring an eco-friendly method.