5 results on '"Xinying, Xiang"'
Search Results
2. Erythrocyte membrane camouflaged graphene oxide for tumor-targeted photothermal-chemotherapy
- Author
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Junhua Zhang, Wansong Chen, Xinying Xiang, Yanjie Wang, Rong Gui, Jiang Jing, Xueyuan Huang, Rong Huang, Xin-Min Nie, Jian Li, and Haiye Jiang
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Drug ,Chemotherapy ,Biocompatibility ,media_common.quotation_subject ,medicine.medical_treatment ,02 engineering and technology ,General Chemistry ,Mononuclear phagocyte system ,Photothermal therapy ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,medicine ,Cancer research ,General Materials Science ,Photosensitizer ,Doxorubicin ,0210 nano-technology ,Indocyanine green ,media_common ,medicine.drug - Abstract
Nanodrug carrier-based cancer therapy has been actively developed in the past decades. The main challenges faced by nanodrug carriers include poor drug loading capacity, rapid clearance from blood circulation, and low antitumor efficiency. In this work, a new type of antineoplastic agents, F-RGID, were developed using a two-dimensional graphene oxide (GO) with an incorporated photosensitizer (Indocyanine Green, ICG) and a chemotherapeutic drug (Doxorubicin, DOX) as the internally wrapped nanoparticles, and the RBC membrane (RM) inserted with the targeting molecule (folic acid, F) as a shell. The endogenous nature of RM confers F-RGID excellent biocompatibility and the ability to evade from clearance by the reticuloendothelial system (RES). In addition, F-RGID is decorated with folic acid for selective recognition of tumor cells via a lipid-insertion approach. Another salient feature of the antineoplastic platform is the combination of photothermal-chemotherapy with ICG and DOX. In vitro and in vivo experiments revealed that F-RGID could provide a bionic nanoplatform for tumor-targeted photothermal-chemotherapy in clinic.
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- 2019
- Full Text
- View/download PDF
3. Aptamer-Based Fluorometric Ochratoxin A Assay Based on Photoinduced Electron Transfer
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Changbei Ma, Han Zhao, Mingjian Chen, and Xinying Xiang
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Ochratoxin A ,Fluorophore ,Guanine ,Health, Toxicology and Mutagenesis ,Aptamer ,lcsh:Medicine ,Electrons ,Food Contamination ,Wine ,02 engineering and technology ,Toxicology ,Photochemistry ,01 natural sciences ,Article ,Photoinduced electron transfer ,photoinduced electron transfer ,chemistry.chemical_compound ,Detection limit ,010401 analytical chemistry ,lcsh:R ,food and beverages ,Aptamers, Nucleotide ,021001 nanoscience & nanotechnology ,Ochratoxins ,Fluorescence ,0104 chemical sciences ,Fluorescence intensity ,Spectrometry, Fluorescence ,chemistry ,quencher-free ,Biological Assay ,fluorescence ,DNA Probes ,0210 nano-technology ,ochratoxin A - Abstract
This study describes a novel quencher-free fluorescent method for ochratoxin A (OTA) detection based on the photoinduced electron transfer (PIET) between guanine and fluorophore. In the absence of OTA, carboxyfluorescein (FAM)-labeled aptamer can partly hybridize with the complementary strand of OTA aptamer (OTA-cAPT), which contains four guanines at its 3&prime, end. As a result, the fluorescence of FAM is quenched due to PIET and stacked guanines. In the presence of OTA, FAM-labeled OTA aptamer can bind specifically to OTA, and thereby the high fluorescence intensity of the dye can be maintained. Under the optimal conditions, the method had a detection limit of 1.3 nM. In addition, the method we proposed is highly sensitive and specific for OTA. Furthermore, the method was proven to be reliable based on its successful application in the detection of OTA in red wine samples. Therefore, this promising, facile, and quencher-free method may be applied to detect other toxins by using other appropriate aptamers.
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- 2019
4. A Novel Detection Method of Human Serum Albumin Based on the Poly(Thymine)-Templated Copper Nanoparticles
- Author
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Kefeng Wu, Mingjian Chen, Changbei Ma, Hailun He, Xinying Xiang, and Hanchun Chen
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Analyte ,Point-of-Care Systems ,Analytical chemistry ,chemistry.chemical_element ,Nanoparticle ,Metal Nanoparticles ,Serum Albumin, Human ,02 engineering and technology ,lcsh:Chemical technology ,01 natural sciences ,Biochemistry ,label-free ,Article ,Analytical Chemistry ,chemistry.chemical_compound ,medicine ,Humans ,lcsh:TP1-1185 ,Electrical and Electronic Engineering ,Instrumentation ,Serum Albumin ,Detection limit ,010401 analytical chemistry ,copper nanoparticles ,021001 nanoscience & nanotechnology ,Human serum albumin ,Fluorescence ,Copper ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Thymine ,body regions ,Fluorescence intensity ,Spectrometry, Fluorescence ,chemistry ,human serum albumin ,embryonic structures ,0210 nano-technology ,Nuclear chemistry ,medicine.drug - Abstract
In this work, we developed a facile fluorescence method for quantitative detection of human serum albumin (HSA) based on the inhibition of poly(thymine) (poly T)-templated copper nanoparticles (CuNPs) in the presence of HSA. Under normal circumstances, poly T-templated CuNPs can display strong fluorescence with excitation/emission peaks at 340/610 nm. However, in the presence of HSA, it will absorb cupric ion, which will prevent the formation of CuNPs. As a result, the fluorescence intensity will become obviously lower in the presence of HSA. The analyte HSA concentration had a proportional linear relationship with the fluorescence intensity of CuNPs. The detection limit for HSA was 8.2 × 10−8 mol·L−1. Furthermore, it was also successfully employed to determine HSA in biological samples. Thus, this method has potential applications in point-of-care medical diagnosis and biomedical research.
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- 2017
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- View/download PDF
5. Identification and Characterization of Lipopolysaccharide Induced TNFα Factor from Blunt Snout Bream, Megalobrama amblycephala
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Yuhong Jiang, Xinying Xiang, Jun Xiao, Huan Zhong, Yina Lv, Jinpeng Yan, Yi Zhou, and Leilei Tang
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Lipopolysaccharides ,0301 basic medicine ,Lipopolysaccharide ,Gene Expression ,lcsh:Chemistry ,chemistry.chemical_compound ,lcsh:QH301-705.5 ,Phylogeny ,Spectroscopy ,Immune response gene ,Fishes ,04 agricultural and veterinary sciences ,General Medicine ,Computer Science Applications ,medicine.anatomical_structure ,Organ Specificity ,lipopolysaccharide stimulation ,Tumor necrosis factor alpha ,innate immune ,DNA, Complementary ,Spleen ,Biology ,Article ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,Complementary DNA ,Megalobrama amblycephala ,lipopolysaccharide induced TNFα factor ,medicine ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Physical and Theoretical Chemistry ,Molecular Biology ,Transcription factor ,Megalobrama ,Innate immune system ,Base Sequence ,Organic Chemistry ,biology.organism_classification ,Molecular biology ,Immunity, Innate ,030104 developmental biology ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,Immunology ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Transcription Factors - Abstract
Lipopolysaccharide induced TNFα factor (LITAF) is an important transcription factor responsible for regulation of tumor necrosis factor α. In this study, a novel litaf gene (designated as Malitaf) was identified and characterized from blunt snout bream, Megalobrama amblycephala. The full-length cDNA of Malitaf was of 956 bp, encoding a polypeptide of 161 amino acids with high similarity to other known LITAFs. A phylogenetic tree also showed that Malitaf significantly clustered with those of other teleost, indicating that Malitaf was a new member of fish LITAF family. The putative maLITAF protein possessed a highly conserved LITAF domain with two CXXC motifs. The mRNA transcripts of Malitaf were detected in all examined tissues of healthy M. amblycephala, including kidney, head kidney, muscle, liver, spleen, gill, and heart, and with the highest expression in immune organs: spleen and head kidney. The expression level of Malitaf in spleen was rapidly up-regulated and peaked (1.29-fold, p < 0.05) at 2 h after lipopolysaccharide (LPS) stimulation. Followed the stimulation of Malitaf, Matnfα transcriptional level was also transiently induced to a high level (51.74-fold, p < 0.001) at 4 h after LPS stimulation. Taken together, we have identified a putative fish LITAF ortholog, which was a constitutive and inducible immune response gene involved in M. amblycephala innate immunity during the course of a pathogenic infection.
- Published
- 2017
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