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This article describes a new idea about using Bifidobacteriumsp isolates as an alternative to antibiotic growth promoters, based on the ability of Bifidobacteriumsp isolates, which have the potential as a probiotic in laying hens. This study aims to prove that Bifobacteriumsp could improve production performance, including feed conversion ratio, feed efficiency, and nutrient intake (feed intake, crude protein intake, crude fiber intake, lipid intake, and organic matter). A total of 150 laying hens at 25 weeks of age were divided into three groups (P0: control, P1: 0.1% antibiotic growth promoters, P1: 0.05% Bifidobacterium spp. probiotic) and fed on a basal diet containing 2750 kcal/kg metabolizable energy and 18% crude protein for four weeks. The different supplementation to the basal diets showed significant differences (p

The purpose of this research was to determine the effect of supplementary feeding with fermented Moringa leaf flour on cholesterol levels of Mojosari duck eggs and to calculate the cost of production. This study used 60 mojosari ducks, layer phase aged 27 weeks. Moringa flour was fermented using probiotics and given in four treatments, namely P0 (100% commercial feed), P1 (0.5% moringa leaf flour fermentation + 100% commercial feed), P2 (1% moringa leaf flour fermentation + 100% commercial feed), P3 (1.5% fermented Moringa leaf meal + 100% commercial feed). The variable observed in this study was cholesterol levels in Mojosari duck eggs. The results showed thatgiving Moringa leaf flour fermentation can give significantly different values (p

The aim of this research was to investigate the effect of probiotics Bacillus subtilis on feed efficiency and egg mass of laying hens. This research was using 36 of 38 weeks-old laying hens Isa Brown strain. The concentration of probiotics B. subtilis was 1 x 109 CFU/ml, there were three kinds of treatments, P0 (without given probiotics B. subtilis), P1 (given 0.1 gram of probiotics B. subtilis per kg of feed), and P2 (given 0.2 gram of probiotic B. subtilis per kg of feed). The results of feed efficiency, the provision of probiotics can provide a significantly different values (p

Eimeria causes coccidiosis, which has long been recognized as a disease in chickens that significantly affects the economy. The global chicken population continues to grow, and its contribution to food security increases, making it increasingly important to produce chicken meat that is safe for human and health. This study aims to prove Pediococcus pentosaceus ABY 118 to modulation of ChIFN-γ and ChIL-10 in chickens infected with E. tenella oocysts. This study used 100 of day-old chickens (DOC), randomly divided into 5 treatments; each treatment consists of 20 chickens. The treatments was as follows: P0 (−): negative control; P0 (+): positive control; P1: monensin; P2: probiotic 1.5 × 108 CFU/ml; and P3: probiotic 3.0 × 108 CFU/ml. At the age of 20 days, Eimeria tenella (E. tenella) oocysts were inoculated orally at a dose of 1 × 104. The probiotic P. pentosaceus ABY 118 was given orally through drinking water from DOC to 35 days. Monensin was given orally through feed from the age of 14–26 days. The results of statistical analysis showed that there was a significant difference (P

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p

The aim of this research was to determine the effect of Cirripedia sp. flour as feed substitution of protein source in feed on the digestibility of dry matter and organic matter in 100 male rex broilers. The research was experimental by using a completely randomized design with four treatments and five replications. Substitution of Cirripedia sp. flour respectively in feed were 0%, 2.5%, 5%, 7.5% given for four weeks. Data collection was carried out in the fourth week (for seven days) which included consumption data, manure weight and analysis of treatment feed and manure analysis. The data were analyzed using Analysis of Variance and the difference between treatments was determined by the Duncan test. The results showed that there was no significant difference (p>0.05) and that there was no significant difference (p>0.05) on the digestibility of rex broilers. This shows that the substitution of Cirripedia sp. flour in feed can replace animal protein sources in fish meal in complete feed rabbits.Key words : Cirripedia sp, digestibility, feed subtitution, flour, rabbit.

Background and Objectives: Use of antibiotics as growth promoters in animal feeds has been restricted due to the residues in poultry products such as egg and meat, furthermore to the antibiotic resistant of pathogenic bacteria. The prohibition of their use opens the opportunity for the use of non-antibiotic feed additives such as probiotics. The objectives of this study were to investigate the effect of the addition of Lactobacillus casei WB 315 and crude fish oil (CFO) to diets on growth performance, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), low density lipoproteins (LDL), high density lipoprotein (HDL), and cholesterol levesl of broiler chickens. Materials and Methods: In this research, one-day old male broiler chicks were used and divided equally into four groups, namely a basal diet without L. casei WB 315 and without CFO (P0), basal diet supplemented with 0.5% L. casei WB 315 of total broiler basal feed (1.2 × 109 cfu/ml) and without CFO (P1), basal diet supplemented without L. casei WB 315 and 1% CFO of total broiler basal feed (P2), and basal diet supplemented with 0.5% L. casei WB 315 of total broiler basal feed (1.2 × 109 cfu/ml) and 1% CFO of total broiler basal feed (P3) for 35 days. Results: The results of addition 0.5% Lactobacillus casei WB 315 (1.2 × 109 cfu/ml) and 1% CFO of total broiler basal feed after 35 days showed significant difference among treatment in feed efficiency (p

Penelitian ini bertujuan untuk mengetahui efektifitas probiotik Lactobacillus casei dan Lactobacillus rhamnosus sebagai pengganti Antibiotic Growth Promoter (AGP) terhadap total kolesterol, Low Density Lipoprotein (LDL) dan High Density Lipoprotein (HDL) pada ayam pedaging. Ayam broiler sebanyak 18 ekor day old chick diacak kedalam enam perlakuan (P0, P1, P2, P3, P4 dan P5) setiap perlakuan terdiri dari 3 ulangan, yakni (P0) 100% pakan basal, (P1) pakan basal+0.01 gram AGP/kg pakan, (P2) pakan basal+probiotik 0.05 gram/kg pakan, (P3) pakan basal+probiotik 0.1 gram/kg pakan, (P4) pakan basal+probiotik 0.025 gram/liter air minum, (P5) pakan basal+probiotik 0.05 gram/liter air minum. Data di analisis menggunakan uji Multivariate Analysis of Variance (MANOVA) dilanjutkan uji Duncan. Hasil menunjukkan bahwa pemberian probiotik berbeda nyata (p

Background and Objectives: An experiment was designed to determine the effect of using lactic acid bacteria as alternative antibiotic growth promoters on external and internal quality of egg’s Coturnix coturnix japonica. Materials and Methods: Coturnix coturnix japonica (n=240, 14 weeks of age) were randomly distributed into six treatment groups. The treatments were P0 (free antibiotic feed), P1 (free antibiotic feed with 1 gram antibiotic growth promoters (AGP)/100kg feed), P2 (free antibiotic feed with 5 gram probiotic/100kg feed), P3 (free antibiotic feed with 10 grams probiotic/100kg feed), P4 (free antibiotic feed with 5 gram probiotic/200L drinking water), and P5 (free antibiotic feed with 10 gram probiotic/200L drinking water). Probiotic contained Lactobacillus casei (L. casei) and Lactobacillus rhamnosus (L. rhamnosus) culture (1.2 x 108 CFU/gram). To assess the quality parameters, twenty eggs were randomly collected from each treatment at the end of the experimental period, and the data were analysed using one way Anova. Results: Results of the external quality indicated that egg’s weight, length, and width, along with the shell weight and thickness were significantly different (P

Aim: The purpose of this study was to know the genetic and biochemical identification of isolated lactic acid bacteria (LAB) from Balinese bovine (Bos sondaicus) intestinal waste, acidity, and ox bile salts and to inhibit the growth pathogen of Staphylococcus aureus and Escherichia coli and the potential of those isolated to improve nutrient value of wheat pollard as animal feed ingredient by fermentation process. Materials and Methods: This research was divided into three stages. The first stage, isolated LAB were obtained from the bovine intestines at a slaughterhouse in Indonesia. Small intestinal samples were collected from 10 healthy Balinese beef cattle (B. sondaicus). The isolated LAB were identified by VITEK 2, polymerase chain reaction, and 16S rDNA. The basic local alignment search tool (BLAST) was performed to determine the phylogenetic tree. The second stage, the LAB were screened for their tolerance at pH 2, 3, and 4; bile salt, and antagonistic to enteric pathogen. In the third stage, to determine the potency of this isolate to increase nutrient content of wheat pollard by facultative anaerobe fermentation for 3 and 5 days. Results: The result of the first stage showed that the isolate could be identified as Lactobacillus casei WPL 315. The result of the second stage showed that the isolate tolerance to low pH (pH 2, pH 3, and pH4) for 90 min and 24 h, and this isolate had viability tolerance in 0.3% bile salt. The isolate can inhibit S. aureus and E. coli. The result of the third stage by proximate analysis showed that crude protein increased by 23.08% after fermentation, while crude fiber decreased by 61.24% on the level 0.5% L. casei subsp. WPL 315 in the 3-day fermentation. Conclusion: Based on the results, it showed that L. casei WPL 315 derived from indigenous intestinal Balinese beef cattle (B. sondaicus) has tolerant characteristic on acidity and ox bile salts, has antagonistic effect against E. coli and S. aureus, and has the ability to increase crude protein and decrease crude fiber content of wheat pollard. It would be interesting to determine whether the strain has a probiotic candidate. Keywords: Escherichia coli, Lactobacillus casei, probiotics, Staphylococcus aureus, wheat pollard.

This study aimed to identified cellulolytic inoculant code WPL 214 isolated from bovine rumen fluid waste of Ongole Cross Breed of Surabaya Slaughter house. A single colony of isolates celulolytic grown on 5 mL of liquid media Luria Bertani (LB) consist of 1 % NaCl , 1% tripton , 0.5 % yeast extract, containing1 % carboxymethyl cellulose (CMC) at temperature 37°C, using a shaker of incubator during 16-18 hours. That isolate determined by 16S DNA gen analysis using High Fidelity Platinum Taq DNA Polymerase with primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ and primer reverse PB38 5’-GMT ACCTTG TTA CGA CTT-3’ for PCR. Nucleotide sequence of 16S DNA fragment was determined through the sequencing method. The result was then compared with GenBank database to recognize the type of the sample bacteria. DNA isolation and 16S DNA coding genes amplification were carried out using Kit High Fidelity Platinum Taq DNA Polymerase. Afterward, BLAST was applied to identify the phylogenetic tree. The bacteria was capable of indicating the existence of clear zone in a media CMC by congo red staining. The existence of the clear zone associated with the activity of microbes to degrade cellulose. The conclusión of this research based on the results was the sequencing nucleotides genome 16S DNA showed that cellulolytic inoculant was identified as Enterobacter cloacae WPL 214. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi lebih lanjut isolat selulolitik kode WPL 214 yang telah diisolasi dari cairan rumen sapi peranakan ongole dari limbah Rumah Potong Hewan Surabaya. Koloni tunggal dari isolat selulolitik ditumbuhkan pada 5 mL media cair Luria Bertani (LB) dengan komposisisi 1% NaCl, 1% tripton, 0,5% yeast ekstrak, yang mengandung 1% substrat carboxymethyl cellulose (CMC) pada suhu 37°C, dengan pengocokan menggunakan shaker incubator selama ±16-18 jam. Penelitian ini terdiri dari dua tahap, tahap pertama dilakukan isolasi DNA, tahap kedua dilakukan identifikasi gen penyandi 16S DNA, amplifikasi DNA dengan polymerase chain reaction (PCR). Amplifikasi gen penyandi 16S DNA menggunakan Kit High Fidelity Platinum Taq DNA Polymerase dengan primer forward PB36 5’-AGR GTT TGA TCM TGG CTC AG-3’ dan primer reverse PB38 5’-GMT ACC TTG TTA CGA CTT-3’ yang digunakan untuk PCR. Hasil sekuensing nukleotida dari 16S DNA selanjutnya dibandingkan dengan urutan nukleotida dari GenBank database untuk dilakukan BLAST untuk mengidentifikasi berdasarkan pohon filogeni. Bakteri tersebut mampu menunjukkan adanya zona bening pada media Carboxymethyl cellulose (CMC) dengan pewarnaan congo red. Adanya zona bening tersebut berhubungan dengan aktivitas mikrob untuk mendegradasi selulosa. Simpulan penelitian menunjukkan bahwa berdasarkan hasil urutan nukleotida genom 16S DNA serta pohon filogeni, maka isolat selulolitik tersebut diidentifikasi sebagai Enterobacter cloacae WPL 214.

Objective: The objective of the present study was to determine the effects of mangosteen peel and ginger rhizome on the performance and cholesterol levels of heat-stressed broiler chickens. Materials and Methods: One hundred unsexed day-old commercial broiler chicks were fed one of four experimental diets. Diet T1, the control treatment, contained neither mangosteen peel nor ginger rhizome, diets T2 and T3 contained 5% mangosteen peel and 5% ginger rhizome, respectively and diet T4 contained 2.5% of both mangosteen peel and ginger rhizome. Results: The results indicated that the consumption of diet T4 was significantly higher than that of the other three diets (p

This study aimedto know the growth activityofcellulolytic bacteria EnterobactercloacaeSAR 1isolatedfrombovinerumen fluidwaste. Isolates that had been cultured were taken as much as 10 mL and thentransferred to100 mL growth medium in Erlenmeyer flask. Culturesuspensions were incubated in a shakerincubator (37°C, 120 rpm). Optical density was measured at ë 600 nm by taking as much as 1 mLsampling with interval of two hours for 24 hours (hour 0; 2; 4; 6; 10; 12; 14; 16; 18; 20; 22; 24). The firstsampling was done at 0thhour and continued until OD values †showed a clear decline. Optical density wasmeasured with a UV-Vis spectrophotometer at wave length ë 600 nm. Growth curve was obtained from theresult of absorbance measurement on the time. Optimum growth production of E.cloacae SAR 1 occurredat the 12thhoursof incubation, optimum temperature of 35°C and optimum pH 6.

Mud crab is one of the fishery commodity which potential to be cultured because it has high economic value. Mud crab has nutrients content such as protein 62,72%, lipid 0,83%, ash 7,5% and water content 9,9%. Trash fish is fresh feed which commonly used as main feed for the growth of mud crab juvenile of 5,3to 13,8%, however Kuniran fish has omega-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) content which is necessary for optimum growth of mud crab.The study method was implemented as the experimental method use Completely Randomized Design (CRD) which consists of five treatments and four replications. The quantity of Crude Fish Oil which is added in trash fish feed on this study are: Treatment A (0%), B (2%), C (4%), D (6%) dan E (8%). The observed parameters are growth of mud crab and feed conversion ratio value. Data analysis use Variant Analysis (ANAVA) and continued with Duncan's Multiple Range Test to determine differences between treatments. The results shows that addition of Crude Fish Oil in trash feed fish for 32 days of rearing gives significantly different effect (P0,05). High length and width growth of carapace were obtained on D and E treatment. The water quality of mud crab during 32 days of rearing is the temperature range of 28-29°C, pH range of 7,5-9,0, salinity range of 15-21ppt, dissolve oxygen range of 4 mg/l and ammonium range of 0,09-0,27 mg/l.

This study aimed at investigating the effect of fermented coffee-peel (Coffea sp.) flour supplementation on the total cholesterol, low-density lipoprotein cholesterol (LDL-c), triglycerides, high-density lipoprotein cholesterol (HDL-c), docosahexaenoic acids (DHA), and eicosapentaenoic acids (EPA) contents in tilapia (Oreochromis niloticus) fillet. Five treatments with four replicates of each were assigned to a completely randomized design (CRD). The treatments were doses of fermented coffee-peel flour added to the commercial feed: P0 (100% commercial feed), P1 (100% commercial feed + 6% fermented coffee-peel flour), P2 (100% commercial feed + 12% fermented coffee-peel flour), P3 (100% commercial feed + 18% fermented coffee-peel flour), and P4 (100% commercial feed + 24% fermented coffee-peel flour). The observed parameters were total cholesterol, LDL-c, HDL-c, Triglycerides, EPA, and DHA of tilapia fillet. The results showed that the supplementation of fermented coffee-peel flour via commercial feed significantly influenced the deposition of total cholesterol, LDL-c, HDL-c, Triglycerides, EPA, and DHA in the tilapia fillet, p

Aim: This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-Dglucanase, and β-glucosidase, at optimum temperature and optimum pH) of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia. Materials and Methods: To produce enzyme from a single colony of E. cloacae WPL 214, 98 × 1010 CFU/ml of isolates was put into 20 ml of liquid medium and incubated in a shaker incubator for 16 h at 35°C in accordance with growth time and optimum temperature of E. cloacae WPL 214. Further on, culture was centrifuged at 6000 rpm at 4°C for 15 min. Pellet was discarded while supernatant containing cellulose enzyme activity was withdrawn to assay endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase. Results: Cellulase enzyme of E. cloacae WPL 214 isolates had endoglucanase activity of 0.09 U/ml, exoglucanase of 0.13 U/ml, and cellobiase of 0.10 U/ml at optimum temperature 35°C and optimum pH 5. Conclusion: E. cloacae WPL 214 isolated from bovine rumen fluid waste produced cellulose enzyme with activity as cellulolytic enzyme of endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase and β-glucosidase. Keywords: endo-(1,4)-β -D-glucanase, exo-(1,4)-β-D-glucanase, β-glucosidase., Enterobacter cloacae WPL 214.

Tilapia is a freshwater fish species are widely cultivated in Indonesia. Tilapia production continues to grow every year, the average increase in the number of experienced production tilapia reaches 22.24% in the 2010-2011 range. Tilapia production in 2011 reached 567.449 tons and was second aquaculture production by major commodity after seaweed. This study aimed to determining differences in extract ether digestibility, organic matter, and energy on feed derived from different factories in tilapia (Oreochromis niloticus) using surgery techniques. The research design is using completely randomized design. The variables were measured extract ether digestibility, organic matter, and energy in tilapia feed. Analysis of the data processed using Analysis of Variance (ANOVA) to determine differences in treatment given. If there is a difference then followed by Duncan's Multiple Range Test with a significance level of 5%. Based on the findings that there were no significant differences (p>0.05) in extract ether , organic matter, and energy digestibility of the feed value of tilapia. The results stated that the feed treatments A, B and C efficiently digested by tilapia.

The purpose of this study was to know the effect of probiotic supplementation to feed consumption, egg production, egg weight, feed conversion, feed efficiency and egg cholesterol. 80 quails of Coturnixcoturnixjaponica at 14 weeks of age were completely randomized into five treatments, each treatment consisted of four replication and each replication consisted by five heads. T0 as a control without probiotic supplementation, T1 with the addition of 1 ml probiotic/kg of feed, T2 with the addition of 2 ml probiotic/kg of feed, T3 with the addition 1 ml probiotic/liter drinking water and T4 with the addition of 2 ml probiotic/liter drinking water. The results showed that the probiotic supplementation gave a significant effect (p 0,05) egg weight and Quail Day Production. T4 showed the highest significantly different in feed conversion, feed efficiency and egg cholesterol. It could be better to give 2 ml probiotic containing Enterobacteria spp., Bacillus spp., Cellulomonas spp., andActinomyces spp. in drinking water for the best result. Keywords: Probiotic, production performance, egg cholesterol, quail

Eel requires unsaturated fatty acids of linolenic acid for growth. Which can be supplied from earthworms. In this study, addition of earthworm in formulation feed aimsed to improve the fatty acid profile eel meat. This research used experimental method and randomized complete design method with five treatments. Each treatment was repeated four times. The use of earthworms in feeding treatment formulation was done for 21 days with different level i.e: 0 % (P0), 25 % (P1), 50 % (P2), 75 % (P3) and 100 % (P4). The result showed that the addition of eartworm significantly influenced the omega 3 contents (EPA & DHA) of eel meat.

This research aims to identify isolate as a probiotic candidate derived from liquor rumen of local beef cattle and to know the ability of isolates as biofermentor on basal feed to the changes in the nutrient value. The selected samples were obtained from a slaughterhouse in Surabaya. This study consisted of two stages. The first stage was the identification of bacteria through the test of morphology, Gram staining, biochemical, resistance to acidity and 16S rDNA sequencing. The second stage was a test of the ability of the isolates on the nutrient of basal feeds by fermentation for three days in an aerobic condition. Based on the findings of the first phase, it has been identified that probiotic bacterium rods, motility positive, Gram-negative, have viability at pH 2 and pH 3 for 90 minutes and 24 hours and have the ability to ferment lactose, sucrose, galactose, ribose, cellobiose and xylose. Furthermore, based on test results of 16S rDNA sequencing, the probiotic bacterium was identified as Bacillus cereus WPL 415. Based on the research results at the second stage, Bacillus cereus WPL 415 at doses of 0.25% and 0.5% could improve the nutrient content of the basal feed. The results of the proximate analysis revealed that there was an increase in crude protein content of 6.78% until 8.12% compared to the control and was able to lower the crude fiber content of 15.19% and 17.40% compared to the control. Based on these results it can be concluded that Bacillus cereus WPL 415 from local beef cattle can be used as a probiotic candidates to improve the quality of animal feed. Keywords: Bacillus cereus, probiotic, crude protein, crude fiber

The discovery and characterisation of indigenous lactic acid bacteria (LAB) are important for diversity microbes as candidate probiotic. This research was aimed to identify lactic acid bacteria isolate from isolation process of local bovine rumen fluid from slaughterhouse in Surabaya Indonesia. Genotypic testing was conducted by analyzing 16S rDNA and biochemical identification. DNA of sample isolate was isolated and then amplified in vitro through the PCR method. Determination of nucleotide sequence of 16S rDNA was performed with sequencing method. The result of nucleotide sequence was than compared with GenBank database. The BLAST was then applied to identify the phylogenetic tree. Based on the biochemical characterization and nucleotide sequences, that isolate was identified as Lactobacillus rhamnosus subsp TG15. The result of this research showed that L.rhamnosus subsp TG15 showed viability bacteria in MRSA as control as much as 1.1 x 10 8 CFU/ml, mean while in MRSA pH 2, L.rhamnosus subsp TG15 showed its viability as much as 9.3 x 10 6 CFU/ml. Viability of isolate on bile tolerance 0.3% was 2.4 x 10 7 CFU/ml). Index antagonist bacteria test on S.aureus showed inhibition diameter as much as 2.0 mm and in antagonist test on E.coli as much as 2.5 mm. Based on the result, it could be concluded that this research found a new strain of lactic acid bacteria, L.rhamnosus subsp TG15 and that isolate has ability as the probiotic candidate. Keywords: L.rhamnosus subsp TG15; survival on acidity; bile salts; S.aureus and E.coli

Penelitian ini bertujuan untuk mengetahui kandungan serat kasar dan protein kasar pada bekatul padi yang difermentasi dengan bakteri selulolitik dan jamur selulolitik. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap pola searah dengan sepuluh perlakuan dan masing-masing perlakuan dilakukan 3 kali ulangan. Ke sepuluh perlakuan tersebut adalah P0: 250 g bekatul + 3% tetes + Bakteri (B) 0% + Jamur (J) 0%; P1: 250 g bekatul + 3% tetes +B.10% + J.10%; P2: 250 g bekatul + 3% tetes + B.20% + J.10%; P3: 250 g bekatul + 3% tetes + B.30% + J.10%; P4: 250 g bekatul + 3% tetes + B.10% + J.20%; P5: 250 g bekatul + 3% tetes + B.20% + J.20%; P6: 250 g bekatul + 3% tetes + B.30% + J.20%; P7: 250 g bekatul + 3% tetes + B.10% + J.30%; P8: 250 g bekatul + 3% tetes + B.20% + J.30%; P9: 250 g bekatul + 3% tetes + B.30% + J.30%. Setelah dilakukan fermentasi selama 7 hari, dilakukan analisis proksimat. Data yang diperoleh selanjutnya diuji dengan analisis variansi, dilanjutkan dengan uji Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa bakteri selulolitik dan jamur selulolitik dapat menurunkan kandungan serat kasar pada bekatul pada perlakuan P2 (28,96%), P3 (29,34%), P8 (29,53%), P4 (29,65%), P7 (30,23%), P6 (30,37%), P9 (30,58%) yang berbeda nyata (P