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Berdasarkan RISKESDAS tahun 2018 dilaporkan bahwa prevalensi jumlah penyakit tidak menular (PTM) masih sangat tinggi, prevalensi penyakit diabetes mellitus di DI Yogyakarta mencapai 2,4% yaitu peringkat dua setelah DKI Jakarta. Prevalensi penyakit PTM lain seperti hipertensi di DI Yogyakarta mencapai 10,68%, kolesterol banyak diderita oleh kelompok usia 55-64 tahun dan asam urat di Indonesia menurut diagnosis medis mencapai 11,9%. Penderita PTM dapat diketahui kondisi kesehatannya melalui screening kesehatan rutin. Screening ini dapat dilakukan untuk pencegahan dan pengendalian PTM. Target dan luaran dari kegiatan ini adalah masyarakat diwilayah kelurahan Panjangrejo dengan luaran berupa hasil pemeriksaan tekanan darah, gula darah, asam urat dan kolesterol. Metode implementasi yang digunakan dengan melakukan penyuluhan kesehatan terkait PTM dengan media LCD dan liflet, serta melakukan pemeriksaan tekanan darah, gula darah, asam urat dan kolesterol. Hasil dari pelaksanaan kegiatan didapatkan terdapat peningkatan pengetahuan masyrakat terkait PTM setelah dialakukan penyuluhan kesehatan, Hasil screening didapatkan 11 orang mengalami hipertensi ringan, 19 orang hiperuresemia, dan 6 orang hiperglikemia dan 9 orang dengan kadar koleterol tinggi. Screening kesehatan sangat dibutuhkan bagi warga untuk mengetahui kondisi kesehatannya dan sebaiknya kegiatan ini dilakukan rutin bekerjasama dengan layanan kesehatan seperti Puskesmas setempat.

Background: Xestospongia sp. is a species of the Demospongiae class that can be used as a medicinal substance. Xestospongia sp is known to have various activities such as anticancer, antibiotic, anti-fungal, cardiotonic, and antimalarial. One of the ways to make the formulation of natural ingredients is the phytosome vesicle technique. This method can be used to increase the penetration of compounds through cell membranes, increase their absorption and increase its bioavailability in the body. Objective: The aim of this study was to obtain the optimum phytosome composition in the ethanol extract of Xestospongia sp. and phosphatidylcholine with approprite vesicle characteristics. Methods: The preparation was carried out by varying the extract and phosphatidylcholine with a thin layer hydration method at a ratio of 1:1, 1:2, 2:1, and 2:2 and and phytosome characterization was carried out in each of these formulas to obtain the optimal formula. Results: In this study, the optimum phytosome formula has met the criteria based on morphological test parameters using an optical microscope obtained by spherical morphology, stability of physical appearance observations stored at 2oC-8oC ± 1oC temperature, pH measurement using a pH meter obtained a pH value of 4.20, and particle size determination using the Particle Size Analyzer (PSA) obtained a particle size of 180.6 nm and particle distribution value (IP) of 0.133. Conclusion: Composition of the optimum phytosome formula in the ratio of extract and phosphatidylcholine 1: 1 with the LUV phytosome category (Large Unilamellar Vesicle)

This study investigated the decolorization of Remazol Black (RBB) using a TiO2 photocatalyst modified by S and Co co-doped TiO2 (S-Co-TiO2) from a single precursor. X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and UV–Vis specular reflectance spectroscopy were used to characterize the photocatalysts. The results revealed that the band-gap energy of the doped and co-doped TiO2 decreased, with the S-Co-TiO2 8% showing the greatest one, and was found to be 2.78 eV while undoped TiO2 was 3.20 eV. The presence of S and Co was also identified through SEM-EDX. An activity study on RBB removal revealed that the S-Co-TiO2 photocatalyst showed the best result compared to undoped TiO2, S-TiO2, and Co-TiO2. The S-Co-TiO2 8% photocatalyst reduced RBB concentration (20 mg L−1) up to 96% after 90 min of visible light irradiation, whereas S-TiO2, Co-TiO2, and undoped TiO2 reduced it to 89%, 56%, and 39%, respectively. A pH optimization study showed that the optimum pH of RBB decolorization by S-Co-TiO2 was 3.0, the optimum mass was 0.6 g L−1, and reuse studies show that S-Co-TiO2 8% has the potential to be used repeatedly to remove colored pollutants. The results obtained indicate that the modification of S, Co co-doped titania synthesized using a single precursor has been successfully carried out and showed excellent characteristics and activity compared to undoped or doped TiO2.

Carthamus tinctorius Linn, also known as safflower, is a plant with the potential of being used in the production of antimalarial drugs. The purpose of this study was to isolate and identify the steroid compounds in the safflower and determine its antimalarial activity in vitro. The isolation process was conducted through extraction and chromatography methods. Then, the characterization of the isolated compounds was conducted through spectroscopic techniques which include Fourier Transform Infrared Spectroscopy (FT-IR), NMR 1-D (1H and 13C-NMR), and NMR 2-D (HMQC, HMBC, and H-H COZY) as well as comparing data with the existing literatures. In addition, the tests conducted were with variations of isolate concentrations (10, 1, 0.1, 0.01, and 0.001 μg/mL) against 3D7 strain of Plasmodium falciparum. Based on the FT-IR spectroscopic data, the steroid compounds isolated from safflowers might be stigmasterols. In addition, the isolates had -OH functional group in the region of 3431 cm-1, C-O in the region of 1053 cm-1, and Csp3-H in regions of 2960, 2934, and 2865 cm-1. The NMR 1-D data showed presence of 29 carbon atoms, while the protons were 48 in number. Furthermore, the IC50 value of the compound was 34.03 μg/mL with a percentage inhibition of 43.92% against the growth of P. falciparum. Therefore, it was classified as inactive agent in inhibiting the growth of malaria parasites, however, it could be used as a marker compound in C. tinctorius Linn extract.

Healthy lifestyle is an indicator and influence on health problems. Self-management in dealing with this must be done productively. When uric acid is produced in excess and the processing of uric acid is decreased, uric acid levels in the blood are higher. To overcome this, non-pharmacological treatment can be done by consuming bay leaves boiled water. In addition, health education is also needed. Health education is one of the effective nursing interventions to increase awareness and knowledge. This study aims to determine the effectiveness of uric acid health education and drinking bay leaf boiled water to control gout. The research method used is pre-experimental design with pre-posttest design approach. The population was 60 respondents. The sampling method included home visits to gout sufferers. The sample was 22 respondents. Wilcoxon test was used for statistical tests. The results of the pre-posttest showed the P value was 0.001, which is less than 0.05. In other words, health education and consumption of bay leaf water effectively reduce uric acid levels. This research is recommended for the whole community because this intervention has minimal side effects, materials and tools are easy to obtain, and cost-effective so that it can be applied independently

This study performed the synthesis of sodium silicate from the volcanic ash of Mount Kelud with various stirring time in the sodium silicate synthesis process. Synthesis of sodium silicate was carried out using alkaline extraction at low temperature. This method is based on the solubility of silica under alkaline conditions and is proven to be more effective than the smelting method. The dissolution of silica contained in the volcanic ash of Mount Kelud was carried out using NaOH solution to form a Na2SiO3 solution. This process also studied the effect of stirring time on the amount of dissolved silica, with the stirring time of 0.5; 1; 1.5; 2; 2.5; 3 and 4 hours. The dissolved silica levels were tested using AAS. The amount of dissolved silica increased sharply with the longer length of stirring time, however for a stirring time of more than 2.5 hours, the dissolution was slow. The study revealed that the optimal result time was obtained from the stirring time of 2.5 hours with dissolved silica content of 19.82%. Keywords: volcanic ash, silica, sodium silicate, stirring time

Fotodegradasi terkatalisis TiO 2 telah terbukti efektif digunakan untuk mengolah limbah organik, sehingga dilakukan kajian fotodegradasi terkatalisis TiO 2 pada limbah cair industri mie soun sebagai upaya untuk mengurangi pencemaran lingkungan di sekitar daerah produksi. Kajian penambahan oksidator H 2 O 2 juga dilakukan untuk meningkatkan efektivitas fotodegradasi. Optimalisasi reaksi fotodegradasi dilakukan dengan optimasi variabel pH, massa fotokatalis, konsentrasi H 2 O 2 , dan waktu penyinaran. Parameter yang digunakan untuk menilai efektivitas fotodegradasi berupa penurunan kandungan Chemical Oxygen Demand (COD) limbah yang ditentukan dengan metode volumetri. Hasil penelitian menunjukkan bahwa limbah cair industri mie soun mempunyai kandungan COD awal sebesar 1920 mg L -1 . Proses fotodegradasi terkatalisis TiO 2 hanya dapat menurunkan kandungan COD limbah menjadi 1480 mg L -1 (22,92%), sedangkan penambahan H 2 O 2 pada sistem fotokatalis TiO 2 dapat meningkatkan penurunan kandungan COD menjadi 1120 mg L -1 (41,67%). Penurunan paling optimal tercapai pada pH 3, penggunaan 10 mg TiO 2 , penambahan 10 mM H 2 O 2 , dan waktu penyinaran UV selama 24 jam. Photocatalytic degradation over TiO 2 has been proven to be effective in treating organic wastewater. So that a study of photocatalytic degradation over TiO 2 in the cellophane noodles industrial wastewater was conducted as an effort to reduce environmental pollution around the production area. Studies on the addition of H 2 O 2 as an oxidizing agents were also carried out to increase the effectiveness of photodegradation. Optimization of the photodegradation reaction was carried out by optimizing the variables of pH, photocatalyst mass, H 2 O 2 concentration, and irradiation time. Chemical Oxygen Demand (COD) was used to assess the effectiveness of photodegradation and was determined by volumetric method. The results showed that the wastewater from the cellophane noodles industrial wastewater has an initial COD level of 1920 mg L -1 . Photocatalytic degradation over TiO 2 process can only reduce the COD level of the wastewater to 1480 mg L -1 (22.92%), while the addition of H 2 O 2 to the TiO 2 photocatalyst system can increase the decrease in the COD level to 1120 mg L -1 (41.67%). The most optimal decrease was achieved at pH 3, the use of 10 mg TiO 2 , the addition of 10 mM H 2 O 2 , and UV irradiation time for 24 hours.

Cadmium is a toxic pollutant that is harmful to the environment and humans. The purpose of this research was to develop a method for cadmium(II) detection using differential pulse anodic stripping voltammetry (DPASV) using a glassy carbon electrode. The developed method was then applied for cadmium detection in the vegetable samples which is water spinach. The developed method was optimized in several parameters such as potential window, deposition potential, deposition time, and scan rate. The developed method for cadmium(II) detection was also investigated in its analytical performance includes linearity, precision, detection limit, and quantitation limit. The optimum conditions for cadmium(II) detection in 0.1 M KCl using DPASV technique obtained such as potential window from -1200 to -100 mV, deposition potential of -1100 mV (vs Ag/AgCl), and deposition time of 360 s. It was obtained good linearity for cadmium(II) detection using the DPASV technique with an R2 of 0.996. The precision was expressed as %SBR with 0.66%. The detection and quantitation limits for cadmium(II) detection were 0.4206 µM~0.0771 ppm and 0.5525 µM~0.1013 ppm, respectively. The developed method was then applied for cadmium(II) measurement in the water spinach sample and the obtained cadmium(II) concentration in water spinach was 0.2399 mg/Kg.

AIM: This study aims to investigate the concentration of cyclic adenosine monophosphate (cAMP), inositol 1,4,5-trisphosphate (IP3), calcium (Ca2+), and the expression phosphorylated myosin light chain (MLC) in Rattus norvegicus scleral fibroblast cells. METHODOLOGY: This study utilized an in vitro experimental study by applying Rattus norvegicus scleral fibroblast cell culture. The cultured cells were divided into control and lens-induced myopia (LIM) groups. The control and LIM culture groups were each divided into five groups, namely, negative control, 0.1 μM acetylcholine, 0.1 μM himbacine, 0.1 μM methoctramine, and 0.1 μM 4-DAMP group. The cAMP, IP3, and Ca2+ concentration were analyzed in the 0th, 5th, 10th, 20th, and 30th. The phosphorylated MLC expression was analyzed using confocal microscope. RESULTS: In the LIM group, the highest cAMP concentration is visible at the 10th min on the himbacine group (0.304 ± 0; p = 0.043) and on the 4-DAMP group (0.346 ± 0; p = 0.043). The highest IP3 concentration is found on the LIM group at the 20th min in comparison to the control group (2503.6 ± 11 vs. 2039.2 ± 2.1; p = 0.046). The highest Ca2+ concentration belongs to the 4-DAMP treatment group from the 5th to the 30th min. The highest average phosphorylated MLC expression value in the LIM group is shown by the 0.1μM 4-DAMP treatment (184.2 ± 37.9c au). CONCLUSION: The regulation of cAMP, IP3, Ca2+, and phosphorylated MLC on the M2 and M3 muscarinic receptor of the scleral fibroblast cells of myopia animal models differs from normal animal models which may be due to interactions of M2 and M3 muscarinic receptor as compensation reaction or crosstalk on myopia induction.

Inflammation is the host's protective response to any stimulus that harms the body. Excessive inflammatory process causes tissue damage. Therefore, an anti-inflammatory agent is needed. The use of natural ingredients, especially sea sponges, is an option to reduce the side effects of anti-inflammatory agents. This utilization is related to the discovery of new agents. So, we tested the effect of the ethanol extract of Petrosia sp. as an anti-inflammatory agent. Animal induced with 1% carrageenan and left for 1 hour. After that the animals were divided into 6 groups (n = 4) and given oral treatment, namely: Group I (normal group); Group II (negative group); Group III (ethanol extract of Petrosia sp. Concentration of 0.05mg/ml); Group IV (ethanol extract of Petrosia sp. Concentration 0.1mg/ml); Group V (ethanol extract of Petrosia sp. Concentration 0.2mg/ml); and Group VI (positive group, Diclofenac Sodium). After 1 hour, the animals were measured for edema volume and plasma TNF-α levels. Based on the research conducted, the ethanol extract of Petrosia sp. decreased edema volume and plasma TNF-α levels in inflammatory mice. The concentration of 0.2mg/mL had a significant effect on the negative control used (p

Ovarian cancer is the 8th most common cancer that occurs in most women. In a 2018 survey by Global Cancer Statistics in 185 countries of all cancer cases, 3.4% were caused by ovarian cancer. Vascular endothelial growth factor is a multifunctional cytokine that stimulates angiogenesis and increases microvascular permeability by binding to receptors located in endothelial cells in blood vessels. Immature platelet fraction (% IPF) is a modern parameter that measures young platelets and reticulation in peripheral blood. The purpose of this study was to look at differences in plasma VEGF and IPF levels between ovarian cancer sufferers and staging of ovarian cancer. This type of research is a cross sectional observational study of VEGF and Ovarian Cancer stage. A total of 18 people with ovarian cancer were sampled before surgery and after surgery an anatomy pathology examination was performed to assess early stage and advanced cancer staging. From 18 patients with ovarian cancer obtained from the early stage ovarian cancer group and advanced stage group. Obtained a median age of 50 (42-63), whereas in the advanced stage group a median age of 51 (19-68) was obtained. There is a significant difference between VEGF levels in early and advanced stage ovarian cancer with a value of p = 0,000. Using the Spearman Correlation test to assess the correlation between tumor size and VEGF levels, p values

Edible film strips merupakan lapisan tipis oral yang mudah saja diletakkan di lidah atau jaringan mukosa mulut yang akan langsung basah karena air liur kemudian akan cepat terdisintegrasi dan larut. Jus herbal kombinasi digunakan sebagai zat aktif yang dibuat menjadi bentuk sediaan padatan untuk meningkatkan masa simpan dari jus. Edible film strips membutuhkan polimer pembentuk film salah satunya yaitu pati kentang (Solanum tuberosum L). Edible film strips dari pati mempunyai kelemahan mudah sobek sehingga perlu penambahan plasticizer yaitu sorbitol. Variasi konsentrasi sorbitol yang digunakan yaitu 0,4%, 0,7%, dan 1%. Dilakukan evaluasi organoleptis, kerapuhan, susut pengeringan, pH, dan disintegrasi. Hasil penelitian edible film strips jus herbal kombinasi menggunakan variasi konsentrasi sorbitol sebagai plasticizer memberikan hasil dapat dibuat menjadi sediaan edible film strips serta pati kentang dapat dijadikan polimer dalam pembuatan edible film strips jus herbal kombinasi

Objectives Phyllanthus niruri has been known as an immunomodulator and also reported to possess an antiviral activity against several RNA viruses, such as hepatitis B virus and hepatitis C virus by inhibiting viral entry and replication. Since the current situation of Coronavirus Disease 2019 (COVID-19) which infected among the world and caused severe disease and high morbidity, it urgently needed to find new agents against COVID-19. Therefore, in silico screening against COVID-19 receptors is carried out as an initial stage of drug discovery by evaluating the activity of phyllanthin and hypophyllanthin, an isolated from Phyllanthus niruri, in inhibiting spike glycoprotein (6LZG) and main protease (5R7Y) which play as target receptors of COVID-19. Methods Molegro Virtual Docker 6.0 used to determine the best binding energy through the rerank score which shows the total energy bonds calculation. Results Phyllanthin and hypophyllanthin demonstrated to possess greater binding affinity toward the COVID-19 inhibition sites than their native ligand. The rerank score of phyllanthin and hypophyllanthin are lower than the native ligands 6LZG and 5R7Y. This result indicated that phyllanthin and hypophyllanthin have a stronger interaction than the native ligands both in spike glycoprotein (entry inhibitor) and main protease (translation and replication inhibitor). Conclusions In conclusion, phyllanthin and hypophyllanthin are predicted to have strong activity against COVID-19 through inhibiting spike glycoprotein and main protease under in silico study. Further research is needed to support the development of P. niruri as inhibitor agents of COVID-19 through bioassay studies.

lbgBackground and Objective:l/bg ligEtlingera alba l/ig(Blume) A.D. Poulsen is one of the plants of the genus ligEtlingeral/ig which is commonly found in Southeast Sulawesi. The research is still lacking, thus, we assumed other species related to ligE. alba,l/ig specifically from the genuslig Etlingeral/ig that provides antioxidant and radical scavenging activity, namely ligEtlingera elatiorl/ig (Jack) R.M. Smith. Thus, this study aimed to assess the antioxidant and toxicity activity as well as its secondary metabolites. lbgMaterials and Methods:l/bg ligEtlingera albal/ig rhizome was extracted with 96% ethanol. The radical scavenging activity was assayed with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and antioxidant activity was assayed with 2,2'-azino-bis-[3-ethylbenzothiazoline sulphonate (ABTS) assay for radical cation decolourizationlig in vitrol/ig. Both Ascorbic Acid (AA) and Trolox were used as positive control. The secondary metabolites were identified by Thin Layer Chromatography (TLC) and LSMS/MS analyzed the difference between compounds. According to results performed with TLC and LCMS/MS. lbgResults:l/bg The extract exhibited antioxidant properties using both DPPH and ABTS method. The LClsubg50l/subg of the extract was 608.42±18.31 mg Llsupgl/supglsupg1l/supg.lig Etlingera alba l/igrhizome extract contains alkaloids, flavonoids, terpenoids and steroids. The compounds detected in the extract were E-p-Coumaric acid aschantin, 2-Methoxyanofinic acid, Chavicol-β-D-glucoside, Myristicanol B, ent-16α,17-Hydroxy-19-kaurenoic acid, 5-Hydroxy-7,8,2'-trimethoxyflavone, Methyl ursolate and Spinasterol. lbgConclusion:l/bg ligEtlingera albal/ig rhizome contains several compounds that might be responsible for antioxidant activity and the extract itself classified as medium toxic.

Autism Spectrum Disorder (ASD) has a digestive disorder called intestinal mucosal hypermeability, it happens because autism children cannot digest foods that contain gluten and casein, this problem can be overcome by implementing the GFCF (gluten-free casein-free) diet. The purpose of this study was to analyze the dream pastry business opportunity, which is a sheet-shaped puff pastry dough applying GFCF diet therapy. This puff pastry dough is in the shape of a sheet so that it is easy to make creation foods according to the autism children’s favorites. Dream Pastry product design is made from MOCAF flour as a source of gluten-free carbohydrates. It combined with Moringa leaf powder as a natural colorant. Dream Pastry's product development analysis consists of developing product concepts and developing business opportunities. The product concept development consists of processing MOCAF, Moringa leaves to the MOCAF-Moringa combination. Meanwhile, business opportunity analysis uses open a business straight in demand and the analysis of STP (Segmenting, Targeting, Positioning). The analysis were carried out using Market Sizing, while the market potential prediction used TAM (Total Available Market) of autism children recorded in Indonesia as many as 16,987 students. Then, for the SAM (Served Available Market), the author chose East Java as the initial market niche for product distribution. Also, the prediction of sales in the first month called SOM (Share of Market) as many as 106 boxes.

This paper deals with the study on the antibacterial activity of TiO2-doped Ag prepared from radiophotography wastewater. The antibacterial agent was prepared by reduction of Ag(I) in the radiophotography wastewater over TiO2 photocatalyst under UV light irradiation and characterized by EDS, XRD, SRUV, and TEM machines. The antibacterial activity in inhibiting the growth of Staphylococcus aureus was examined by counting the number of viable bacterial colonies using the TPC method. The result shows that Ag doping on TiO2 as TiO2-Ag can shift its absorption into the visible region. TiO2-Ag assigns better antibacterial activity compared to TiO2 under visible light irradiation. The efficiency of the antibacterial activity is found to be influenced by Ag loaded in the TiO2, irradiation time, and the antibacterial agent dose. The highest antibacterial activity is achieved by 100 mg/L of TiO2-Ag (2) under 3 h irradiation by visible light.

Introduction: Monosodium glutamate (MSG) is one of the most widely employed food enhancers. Although the umami compound, controversy persists regarding the effects of MSG intake on body weight. Chronic MSG intake may result in excessive body weight gain and obesity. Consumption of MSG result in organ damage, cardiovascular disease, oxidative stress, and also risk factors for obesity. This study aims to determine the effect of oral MSG on obesity in adult male Wistar rats (Rattus norvegicus).Methods: This true experimental study used the post-test control group design. Twenty-four adult male Wistar rats were randomly divided into four groups: control (received distilled water), Group 1 (MSG 0.378 mg/gr BW), Group 2 (0.756 mg/gr BW) and Group 3 (1.512 mg/gr BW). The obesity parameter was obtained by the Lee index. Kruskal-Wallis test follows by Mann-Whitney test were used to compare the Lee index between groups.Results: Lee’s index mean for each group was 358.4%, 314.1%, 287.8%, and 320.9%, respectively. The Kruskal Wallis test showed a significant difference in the Lee index between groups (p = 0.043). A follow-up test using Mann-Whitney found a significant difference between group 2 and the control group (p = 0.043, p

BACKGROUND: Posterior capsule opacification (PCO) often occurs after cataract surgery. Metformin has been known to have an ability to inhibit fibrosis. This study aimed to investigate the effects of metformin on cell contractility, collagen deposition and degradation in human lens epithelial cells (HLEC) of cataract patients. METHODS: HLEC were isolated from the anterior lens capsule of patients undergoing cataract surgery. The HLEC culture was carried out using explant culture technique. The in vitro PCO model was created by scratching technique on HLEC cultures. The treatment groups were given 0.1, 0.5 and 1 mM metformin, respectively, while the control group were given 10% fetal bovine serum (FBS). On the 7th day after scratching technique, the collagen deposition, collagen degradation and cell contractility were evaluated. RESULTS: Collagen deposition in HLEC was significantly reduced after given 0.1 mM, 0.5 mM and 1 mM metformin (17.92±6.16 mg/mL, 12.92±4.31 mg/mL, 11.25±5.30 mg/mL, respectively), compared to the control group (31.46±7.52 μg/mL, p =0.002). Collagen degradation significantly was increased in the 0.1 mM, 0.5 mM and 1 mM metformin groups (4.77±9.27 mg/mL, 6.59±1.16 mg/mL, 6.35±1.90 mg/mL, respectively) compared to the control group (2.21±2.78 μg/mL, p=0.002). While, collagen contractility in HLEC was significantly reduced in 0.1mM, 0.5mM and 1 mM metformin groups (16.39±3.89%, 13.89±2.59%, 11.93±2.44%, respectively), compared to the control group (44.25±4.95%, p =0.000). CONCLUSION: Metformin reduced collagen deposition and contractility, but increased collagen degradation in HLEC of cataract patients through mechanism of extracellular matrix remodeling. KEYWORDS: metformin, human lens epithelial cell, fibrosis

Deoxyelephantopin is a lactone sesquiterpene compound that shows toxic effects on some cancer cells, otherwise, it is safe on normal cells. The combination of chemotherapy with this compound is intended to determine its effect in increasing the sensitivity of chemotherapy to MCF-7 cancer cells. Cell viability was determined through the MTT method (3-(4,5-dimethyl thiazol-2-il) -2,5-diphenyltetrazolium bromide) to determine the combined effect, while the number of cell deaths was determined through trypan blue staining. Giving deoxyelephantopin-doxorubicin combination to MCF-7 cells showed a synergistic effect with a CI < 0.7. The number of cells that died in the 1.52x and 2.12x combination treatments was higher than the single doxorubicin treatment each at IC50 and ½ IC50 concentrations, this confirms the synergistic effect of the combination. This research proves that deoxyelephantopin can increase the sensitivity and effectiveness of doxorubicin chemotherapy against MCF-7 breast cancer cells.

Synthesis of alginate/zeolite membrane with the glycerol plasticizer for separation of CH4 towards CO2 has been carried out. This study aimed to investigate the effect of glycerol addition towards physical and mechanical properties of membrane on the permeability and selectivity of CH4/CO2. Membrane fabrication was done with mixing of alginate and activated zeolite in the aquabidest with glycerol addition as plasticizer with mass ratio of alginate:glycerol 1:0, 1:0.25, 1:0.5, 1:1 and stirring for 24 h and templeting at room temperature. Membrane formed was tested with FTIR, XRD, SEM, Texture Analyzer and Permeation gas cell. Result showed that glycerol addition into alginate can decrease tensile strength and increase elongation value for about 20% on the mass ratio alginate:glycerol 1:1. Besides, gas permeation rate was also influence by the glycerol addition. The highest permeability of CH4 was obtained with alginate:glycerol 1:0,25 and with permeation rate 1.03 × 10-4 cm3 (STP) cm-2 s-1 cmHg-1. Meanwhile, the highest permeability of CO2 was obtained with the mass ratio alginate:glycerol 1:0,5 with permeation rate about 1,19 × 10-5 cm3 (STP) cm-2 s-1 cmHg-1. The highest selectivity indicated that separation of CH4/CO2 occurred well about 49.2 for alginate:glycerol 1:1.

The bio-oil produced from pyrolysis of woody biomass typically shows unfavourable characteristics such as high acidity, hence it becomes highly corrosive. An upgrading process, e.g., esterification, is necessary to improve the bio-oil quality prior to its use as a transportation fuel. In this work, the bio-oil was produced through a fast pyrolysis of Sengon wood in a fixed-bed pyrolyser at various temperatures. The characteristics (density, viscosity, total acid number, relative concentration of acetic acid, etc.) of the bio-oil were evaluated. The bio-oil with the highest acidity underwent an esterification catalysed by Indonesian natural zeolites at 70 oC for 0-180 min with a ratio of bio-oil to methanol of 1:3. The catalytic performance of the Indonesian natural zeolites during the esterification was investigated. A significant decrease in the total acid number in the bio-oil was observed, indicating the zeolite catalyst’s good performance. No significant coke formation (0.002-3.704 wt.%) was obtained during the esterification. An interesting phenomenon was observed; a significant decrease in the total acid number was found in the heating up of the bio-oil in the presence of the catalyst but in the absence of methanol. Possibly, other reactions catalysed by the Brønsted and Lewis acids at the zeolite catalyst surface also occurred during the esterification.

Carbofuran is a pollutant compound derived from the pesticide Furadan 3GR which is widely used in agriculture. Various methods of carbofuran degradation have been carried out, one of which is the conventional electrochemical method. This study used an electrochemical peroxidation process to degrade Carbofuran in the pesticide Furadan 3GR. This study aims to determine the optimum conditions (time, Na 2 SO 4 concentration, and volume addition of H 2 O 2 ) for electrochemical peroxidation and to compare the effectiveness of electrochemical and electrochemical peroxidation methods by measuring the parameter of carbofuran COD reduction. The significance of the electrochemical peroxidation method and the conventional electrochemical method was compared as a preliminary test. The COD reduction of Carbofuran using traditional electrochemical methods and electrochemical peroxidation was 45.76% and 88.70%, respectively. Batch carbofuran electrochemical peroxidation process was accomplished to ascertain the optimum conditions under various operation times, the concentration of Na 2 SO 4 , and the additional volume of H 2 O 2 . The largest COD reduction of 93.78% was obtained at 10 minutes, 75 mM Na 2 SO 4 , and 2 mL H 2 O 2 . The UV-Vis spectrophotometric absorption of Carbofuran at a wavelength of 274 nm was significantly reduced from 1.377 to 0.131 at optimum conditions. The IR spectrum measurement results indicate a reduction in absorbance for the N-H group (3383 cm-1) and the C=O group (1643 cm-1) at optimum conditions. Overall, the electrochemical peroxidation process proved to be an appropriate technique for COD reduction of Carbofuran in Furadan 3GR pesticides.

Ducks are still less liked by consumers because the quality of production is still low compared to broilers. To improve this quality, additional feed can be in the form of herbs which are thought to have content for this. Asam gelugur leaves contain hydroxycitric acid and active substances that act as antioxidants. So that it is expected to improve the quality of the carcass. The purpose of this study was to examine the effect of giving asam gelugur leaf meal on the percentage and composition of male and female ducks in the grower phase. The study was conducted for 2 months in the Poultry Farm of Faculty of Agriculture, Djuanda University. The 40 grower ducks consist of male and female. Fourty male and female grower ducks were allocated into a 2 x 4 completely randomized factorial design. Factor A consisted of sexes (male and female) and factor B consisted of levels of asam gelugur leaf meal inclusion in rations (0, 2, 4, and 6%). five replicates were used for each treatment. The variables observed were slaughter weight, carcass weight, carcass percentage, commercial cut percentage of carcass and duck carcass composition. The results showed that there was no interaction and there was no significant difference (P> 0.05) from giving asam gelugur leaf meal to carcass percentage, carcass commercial cut percentage and duck carcass composition. The conclusion of this study is that giving asam gelugur leaf meal to males and females can maintain the quality of the carcass and the composition of the local duck carcass.

Chromium (Cr) is commonly used as an anti-corrosive agent. Due to its unique nature, Cr is used to coat metal in order to make it looks shiny and attractive. However, hexavalent chromium (Cr (VI)), a form of chromium used in electroplating, is classified as carcinogenic substance. Its portal of entry into the human body can be through inhalation, ingestion, and dermal absorption. Talang District in central-Java, Indonesia is a center of informal sector’s metal electroplating industry. Electroplating workers in the informal sector rarely wear personal protective equipment (PPE) while working. This research analyzed the relation between chromium exposure, age, length of employment, and nutritional status with renal function of metal electroplaters. The subjects of this study involved 35 electroplaters from Talang with working experience ranging between 6 and 40 years. The independent variables were age, length of employment, nutritional status and exposure to chromium. Dependent variable was workers’ renal function. Urine samples were collected to determine chromium exposure and renal function based on urinary chromium and creatinine levels. Data was analyzed using chi-square test with SPSS Statistics software. The average urinary chromium and creatinine levels were 21.16 μg/L and 209.75 mg/dL respectively. This study found no relation between age and chromium levels in urine and renal function. However, length of employment and nutritional status were found to be significantly related to renal function. Although chromium exposure does not appear significantly associate with renal function, the average urinary chromium levels of metal electroplaters did exceed threshold values.

4-Aminobiphenyl (4-ABP) may cause DNA damage in human liver cells (HepG2 and L-02). Propolis exhibits antioxidant properties through reactive oxygen species (ROS) scavenging. We determined the effects of propolis in alleviating 4-ABP -induced DNA damage using the comet assay. Results revealed that propolis could significantly alleviated oxidative damaged DNA by 4-ABP. Furthermore, we proved that inhibition of cytochrome P450 2E1 (CYP2E1) expression by propolis could contribute to the decreased oxidative DNA damage in the treated cells, as the conversion of 4-ABP into its metabolite, N-hydroxy-ABP (HOABP), was blocked; after all, HOABP showed more genotoxic than its parent chemical, 4-ABP. With the homologous recombination assay, propolis failed to induce DNA repair enzymes. Furthermore, the expression of RAD51, Ku70/Ku80, and OGG1 in treated cells were determined with the western blot, revealing that the expression of these protein were unchanged in comparison with those in nontreated cells. However, propolis could protect the treated cells from DNA damage. In conclusion, propolis could antagonize 4-ABP-induced oxidative DNA damage though the removal of ROS and inhibition of CYP2E1 expression in the treated cells.

The entry of nutrients (nitrate, phosphate and silicate) from land-based anthropogenic activities (industry, agriculture and household waste) through rivers can potentially cause changes in water quality and will affect fertility and aquatic organisms such as phytoplankton. These three nutrients play an important role in the living tissue cells of living organisms and in the photosynthesis process. This study aims to analyze the effect of nitrate, phosphate and silicate content on the plankton abundance in the estuary of the Batang Arau River, Padang, West Sumatra. The method used is a survey method, the determination of the sampling station is done by purposive sampling, data were analysed by Excel and SPSS Software. Based on the results of laboratory analysis, the nitrate content ranged from 0,117 to 0,485 mg/l, phosphate content from 0,239 to 0,704 mg/l, and silicate content from 0,031 to 0,685 mg/l. Planktonic diatom abundance in the estuary of the Batang Arau River ranges from 194 to 750 ind/l. The results of the statistical analysis of the effect of nitrate, phosphate and silicate with an abundance of diatoms are expressed by the equation Y = 743,019 - 481,828Nitrate - 199,552Phosphate - 172,971Silicate. This equation explains that the effect of nitrate, phosphate and silicate content on diatom abundance has an inverse or negative relationship, where nitrate, phosphate and silicate levels increase, the diatom abundance will decrease. The difference of content and the relationship between parameters analyzed in this research are discussed.

Lantana camara Linn is a family of Verbenaceae which grows wild and is widespread in various both tropical and sub-tropical countries. Isolation essential oil of the L. camara leaves extracted by hydrodistillation and were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results of GC-MS analysis show isocaryophyllene (14.39%), ρ-cymene (8.17 %), β-cubebene (7.8 %), α-pinene (7.64 %), and β-elemene (5.51 %) as the main compound. The cytotoxic activity of the isolated essential oil was highly toxic both to Artemia salina shrimp larvae (LC50 15.92 µg/mL) and to breast cancer cells T-47D (IC50 10.67 µM).

Oral mucosal inflammation is one of the oral diseases causing pain and reducing the quality of human life. The types of oral mucosal inflammation that commonly found were recurrent aphthous stomatitis (RAS) and oral mucositis (OM). Anti-inflammatory drugs, both synthetic and plant-based, have been used to treat RAS and OM. Plant-based drugs have been attracted the attention of some researchers to minimize the side effects of synthetic drugs. However, a comprehensive review addressing the use of plant-based drugs for RAS and OM therapy, including drug formulation and species of plant, has not yet been reported. Here, we reported the article review of 9 publications derived from the databases of PubMed, ScienceDirect, Cochrane Library, and other additional relevant works, in order to find the effectiveness and safety of plant-based drugs for RAS and OM therapy. This review was written by following the PRISMA guidelines, and the risk of bias of the articles was evaluated using the Oxford Quality Scoring System. It was found that the effective and safe drugs for RAS therapy contained acemannan from Aloe vera and curcumin from Curcuma longa, both in an oral gel formulation. For OM therapy, drugs contained curcumin from Curcuma longa; licorice from Glycyrrhiza glabra; Aloe vera and black mulberry from Morus nigra, in soft tablet, mouthwash solution or mucoadhesive film formulation. In conclusion, the most effective and safest plant-based therapy for RAS is Acemannan 0.5% in oral gel, whereas for OM is Licorice root extract 0.18 mg in mucoadhesive film.

Kaempferia galanga L. rhizome (KGR) has been used empirically in Asian countries, particularly Indonesia, to treat inflammation. Ethyl para-methoxycinnamate (EPMC) and kaempferol, two phytochemicals contained in KGR, are scientifically proven in playing a role as anti-inflammatory agents. Several studies have explored the pharmacology activities of EPMC and kaempferol, thus a further exploration of the physicochemical properties of the ethanol extract of KGR (EEKGR) is needed. This study aims to confirm the presence of EPMC and kaempferol in EEKGR using spectrophotometric and chromatographic analysis. The KGR was purchased from Buniayu Plantation in Subang, West Java, Indonesia and was identified at Herbarium Bandungense, School of Natural Science and Technology (SITH), Bandung, Indonesia. EEKGR was prepared by cold extraction technique 3x24 hours using 70% ethanol, then was rotary-evaporated to a viscous consistency. The yield of the extract produced was 14.55% w/w (72.7935 g viscous extract from 500 g dried powder of rhizome), with a water content of 4.37% (thermogravimetry method). Phytochemical screening revealed the presence of phenolics and flavonoids. Thin Layer Chromatography (TLC) indicated that EPMC might be present in the EEKGR (Rf = 0.92 compared to that of standard EPMC = 0.92), and kaempferol (Rf = 0.26 compared to that of standard kaempferol = 0.25). The spectrophotometric analysis of EEKGR confirmed the presence of benzoyl and cinnamoyl bands, which positively belongs to flavonoid (UV spectrum = 200-400 nm). The High-performance Liquid Chromatography (HPLC) analysis of EEKGR proved chromatogram peak at 7.2 min which is similar with the standard EPMC (detection was set at 308 nm), however, no chromatogram peak of kaempferol at 4.0 min was observed. Generally, these spectrophotometric and chromatographic analysis results proved that EPMC is present in fair amount in the EEKGR therefore, this extract is interesting to be developed as a biomaterial for drug candidate, particularly to treat inflammation.

Andrographis paniculata is one of the medicinal plants used for the treatment of antidiabetic. Cultivation ages and solvent extraction affected metabolites' composition and concentration that directly cause the plant's efficacies. This research aimed to distinguish A. paniculata based on cultivation ages and solvent extraction using data fusion of UV-Vis and FTIR spectra combined with principal component analysis (PCA). A. paniculata with 2, 3, and 4 months post-planting were extracted by water, 50% ethanol, 70% ethanol, and ethanol. In each extract, we measured UV-Vis and FTIR spectra. Then, we used the data fusion from both spectra. We used UV-Vis and FTIR absorbance from 200–400 nm and 1800–400 cm–1, respectively. Each extract gives a similar pattern of UV-Vis and FTIR spectra, only differ in their intensities. PCA score plot in two and three-dimensional showed A. paniculata extracts could be distinguished based on cultivation ages and solvent extraction with a total variance of 86 and 92%, respectively. Furthermore, this study confirms the data fusion of UV-Vis and FTIR spectra could distinguished A. paniculata extracts combined with chemometrics based on cultivation ages and solvent extraction.

Mangrove has numerous ecological functions, such as a habitat for various biota, a place of care and rearing, with a microclimate regulator and spawning. This ecosystem can store the highest carbon compared to tropical, subtropical, and boreal forests. This research aimed to model the estimation of mangrove carbon stocks using drone images. The method used consists of several steps as follows: (1) Taking and analyzing drone images, (2) Identification and estimation of biomass with carbon stocks, (3) Modeling of mangrove carbon stock using drone and field data. The results of mangrove carbon estimation using logarithmic regression of drone images were the best, by the equation y = 0.0455ln (x) + 0.244. Therefore, the results showed that the R2 value was 0.7454, with the RMSE accuracy-test being 689.9 kg, at 35.4%.Keywords:DronesMangroveCarbon stock

Oil palm press fibre is a byproduct of agro-industry that has a low nutrient content. To improve the nutritional quality of oil palm press fibre, it is carried out with biological processing techniques utilizing the work of indigenous microorganisms derived from local microorganisms of it. The research was conducted experimentally using a completely randomized design (CRD) factorial pattern with 2 factors (3 x 3), namely first factor such as dose of local microorganisms (D 1 = 1%, D 2 = 3% andD 3 = 5%) and second factor such as long of fermentation (L1 = 7 days, L2 = 14 days and L3 = 21 days), with 3 replications. The parameters of this study consisted of the nutritional content of oil palm press fibre fermented such as moisture, dry matter, crude protein, crude fat, crude fiber, ash, and BETN. Based on the results of the study, it was found that the combination of treatment with a dose of local microorganisms 5% and fermentation time of 14 days had a significant effect (P0.05) in increasing the dry matter content and BETN and reducing water content. , crude fat, crude fiber and ash. Giving various doses of local microorganisms and fermentation time can increase crude protein content and have not been able to increase dry matter and BETN content and reduce moisture content, crude fiber and ash.

Objective: Circulating EPCs (endothelial progenitor cells) play a role in neovascularization and vascular repair. Oxidative stress impairs endothelial progenitor. Flavonoid is a phytochemical compound for antioxidant activity. Flavonoid effects toward oxidative stress, apoptosis, and expression of the cell markers on EPCs are not fully understood. This study was aimed to elucidate the effects of quercetin, kaempferol, and myricetin toward oxidative stress, apoptosis, and cell markers of peripheral blood-derived-EPCs. Methods: EPCs (endothelial progenitor cells) were isolated from peripheral blood mononuclear cells (PBMNCs) using cultivation under EPCs spesific media. Oxidative stress in EPCs was induced by H2O2 and then treated by quercetin, kaempferol, and myricetin. Cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, while intracellular reactive oxygen species (ROS), apoptosis and characterization of cells, which expressed CD133 and KDR, was measured using flow cytometry. Results: Quercetin, kaempferol, and myricetin at concentration 12.50 µmol/l were not toxic on EPCs as the cells viability were 96.11±4.03%, 95.42±7.75%, and 94.22±9.49%, respectively. Flavonoids decreased intracellular ROS level in EPCs (quercetin: 14.38±1.47%, kaempferol: 20.21±6.25%, and myricetin: 13.88±4.02%) compared to EPCs treated with H2O2 (30.70%±1.04). Percetage of EPCs apoptosis was not significantly different among each treatment. Immunophenotyping showed the increasing of CD133 and KDR expression in EPCs treated with flavonoids. Conclusion: Quercetin, kaempferol, and myricetin were safe for EPCs, decreased ROS levels, and increased CD133 and KDR expression. However, the flavonoids did not significantly affect EPCs apoptosis.

Objective: Inflammation can be induced by microbiological, chemical, physical factors and plays roles in inflammatory diseases. Turmeric (Curcuma longa L.) has been widely used to provide a diverse array of biological activities, including anti-inflammatory, antimicrobial, also antioxidant. The Turmeric extract (TE) anti-inflammatory potential was conducted using a Lipopolysaccharide (LPS)-induced RAW264.7 macrophage cell line by inhibiting inflammatory mediators especially IL-6, PGE-2, IL-1β, COX-2, TNF-α, iNOS, also NO level. Methods: The TE safe concentration in LPS-induced macrophage cell line was measured using MTS assay for further assay. The inflammatory markers (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO) were measured using ELISA assay and NO by the nitrate/nitrite colorimetric assay in LPS-induced RAW264.7 cell line. LPS induced inflammatory marker by increasing inflammatory marker (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO). Results: TE with 100 to 25 µg/ml, caused a significant reduction of cells viability, reaching only 30.27 % live cells. TE with lower concentrations (7.5; 5; 2.5 µg/ml) had no cytotoxic effect on macrophage cells (viability 117.31-131.08 %). LPS induction caused an increase in inflammatory cytokines IL-1β, PGE-2, IL-6, COX-2, TNF-α as well as iNOS and NO. Turmeric extract caused the reduction of the inflammatory cytokines in a dose-dependent manner. Conclusion: The research resulted that TE has anti-inflammatory activity by decreasing IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, and NO level on LPS-induced RAW264.7 cells.

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.

The present experiment investigated the impact of administrations of lactic fermented turmeric, black pepper or a mixture of both into diets on the hematological parameters and intestinal ecology and morphology of broilers. Three hundred and ninety two broilers were spread to T0 (control), T1 (feed administrated with 1% lactic fermented turmeric), T2 (feed administrated with 1% lactic fermented black pepper) and T3 (feed administrated with 1% lactic fermented turmeric and 1% lactic fermented black pepper). Blood sampling was conducted at days 21 and 35, while intestinal sampling was at day 35. At day 21, erythrocyte counts were greater (P ≤ 0.05) for T1 compared to that for T2 and T3. Broilers in T1 had deceased (P ≤ 0.05) serum total triglyceride compared to that of T0 and T3. The concentration of globulin was reduced (P ≤ 0.05) in T3 than that in T0 and T1. At day 35, serum triglyceride level was more elevated (P ≤ 0.05) in T0 compared to that in T2 and T3 groups. Serum cholesterol as well as low-density lipoprotein levels were lower (P ≤ 0.05) in T3 than that in T0. Compared to other broilers, total protein and globulin concentrations were lower (P ≤ 0.05) in T3. A bigger (P ≤ 0.05) ratio of albumin to globulin (A/G) was seen in T3 compared to that in T1 and T2. Creatinine was greater (P ≤ 0.05) in T1 than in others. The titer of antibody toward vaccine of Newcastle disease was elevated (P ≤ 0.05) in T1 and T2 than in T0. The LAB counts were enhanced (P ≤ 0.05) in ileum of T1 and T2 than that in T0. Compared to T0, the villi height of jejunum was elevated (P ≤ 0.05) in treated broilers. In conclusion, administration of fermented turmeric powder improved erythrocyte counts, antibody titer toward Newcastle disease vaccine, LAB population and jejunal villi height of broilers.

Strawberries are often transported and sold without temperature control, which causes huge losses due to rapid decay and quality deterioration. In this study, the effectiveness of edible coatings using alginate and enriched with an antibacterial agent (cinnamaldehyde from Cinnamomum burmannii essential oil), to maintain the quality and shelf life of strawberry was examined. Alginate coatings were prepared by dissolving 2%(w/v) sodium alginate, 0.5%(v/v) glycerol, 0.1%(w/v) CMC, and 0.5% (w/v) stearic acid, while antimicrobial agent was prepared by homogenizing 0.5%(v/v) cinnamon essential oil and 0.2%(v/v) Tween 20. Physicochemical and biochemical attributes [weight loss, firmness, color (L*, a*, b*), soluble solids content, vitamin C loss, acidity level, odor, total yeast and molds, and total mesophilic microorganisms were analyzed during storage under tropical conditions at 27±2°C. Statistical analysis was performed using ANOVA followed by a Tukey test. The inhibitory activity of antimicrobial agent was evaluated using the disc diffusion method. An alginate-based coatings enriched with Cinnamomum burmannii essential oil (AL+CBEO) was effective in maintaining physicochemical quality attributes and reducing the number of spoilage microorganisms, while alginate coatings alone (AL) found to be optimal for vitamin C loss prevention and additionally exhibited a more desirable odor. Overall, strawberry stored under tropical conditions appeared to be better preserved by alginate-based coatings enriched with cinnamon essential oil.

A B S T R A C TSystemic lupus erythematosus (SLE) is a chronic systemic autoimmune diseaseand can attack almost all organs and tissues of the human body. Beside geneticfactors, environmental factors are thought to play a role, such as ultraviolet light,viral infections and smoking, causing a breakdown of self-tolerance which cantrigger an autoimmune response. The study was conducted in the outpatient andinpatient units of the Rheumatology Division of the Department of InternalMedicine Saiful Anwar General Hospital/Faculty of medicine UniversitasBrawijaya, Malang. Subjects were female patients, aged > 18 years who had beendiagnosed as SLE by internist-rheumatologist based on the 1997 ACR criteria, withSLEDAI score > 3. After 3 months of supplementation, there was a significantdecrease in serum levels of the three pro-inflammatory cytokines (IL-6, IL-17, IFN-gamma), as shown in table 3, compared to before treatment, as well as anti-dsDNA levels.Serum TGF-beta1 levels increased significantly, while 25 (OH) D3 levels also increasedsignificantly.

This study aims to assess BSF maggot flour’s quality using water hyacinth growing media and fermentedfruit waste using EM-4 and molasses. Held a total of 70 g of baby maggot was in the temple for 15 days.Eldest the feed used using fermented water hyacinth and fermented fruit waste with four treatmentsconsist of P0= 0% fermented water hyacinth+ 100% fermented fruit waste, P1= 25% fermented waterhyacinth+ 75% fermented fruit waste, P2= 50% fermented water hyacinth+ 50% fermented fruit waste,and 75% fermented water hyacinth+ 25% fermented fruit waste. The design of the experiment uses acompletely randomized design (CRD). The results showed that substitution of feeding 25% fermentedwater hyacinth+ 75% fermented fruit waste influenced the yield of BSF maggot flour (P 0.05). The best results’ (substitution of feeding25% fermented water hyacinth+ 75% fermented fruit waste) has proximate content of dry matter89%, ash 11%, crude protein 35.5%, crude fat 12%, calcium 7%, nitrogen-free extract material 7%,metabolist energy 2939.64 kcal/ 100 g.

Screen-printed carbon electrode (SPCE) is one of the most interesting designs to combine a working (from carbon based material), reference, and counter electrode in a single-printed substrate. SPCE has been used in many electrochemical measurements due to its advantages for analysis in microscale. This paper summarises the main information about SPCE fabrication from the material and fabrication technique aspect on the flat substrate based on the work that has been published in the last 30 years. The success of SPCE fabrication is highly dependent on the composition of conductive ink which consists of conductive materials, binder, and solvents; substrate; and fabrication techniques. Among the carbon-based materials, the most widely used for SPCE fabrications are graphite, graphene, and carbon nanotubes. The frequent binder used are polymer-based materials such as polystyrene, polyaniline, poly 3,4-ethylenedioxythiophene:polystyrene sulfonate (PEDOT:PSS), and polyvinyl chloride. The solvents used for SPCE fabrication are varied including water and various organic solvents. The main characteristics of the SPCE substrate should be inert in order to avoid any interferences during electrochemical measurements. The screen printing and inkjet printing technique are preferred for SPCE fabrication due to easy fabrication and the possibility for mass production of SPCE.

Gold silica nanocomposite-based biosensors are performing well in sensor technology for biosensor development. Due to this biosensor has good selectivity, excellent conductivity, large surface area, efficient enhancement of electron transfer between enzymes and electrodes and good biocompatibility. Therefore, gold silica nanocomposite can be an ideal matrix for immobilization of biomolecules. This review describes the method of synthesizing gold silica nanocomposites and their characterization, interaction with biomolecules and application of gold silica nanocomposites in electrochemical biosensors.

Soil reinforcement method is one of attempt to improve technical characteristic from the soil, such as soil bearing capacity, compressibility and permeability. The Soil Column Method is one of alternatives to enhance physical characteristic by way of stabilization to improve soil bearing capacity. Rice Husk Ash (RHA) contains high silica element, Calcium Carbide Residue (CCR) contains high calcium which is able to form pozzolan when mixed upon silica. This research aims to improve soil bearing capacity by using column soil method with a mixture of soft soil, 3% Calcium Calbide Residue (CCR) and 12% Rice Husk Ash (RHA). Soil column in this research applied a single column variation with a diameter of 3,2 cm which each has 40 cm, 46 cm, and 53 cm in length and each column with diameter of 3,2 cm, 4,2 cm, and 4,8 cm. Based on the research, ultimate Bearing Capacity (qu) of soft soil without soil column was 54,03 kPa and after being given reinforcement had increased the bearing capacity value (qu). The greates increase in soil bearing capacity of the soft soil occurred in soil column variation of 53 cm in lenght with 4,8 cm in diameter where the soil bearing capacity had increased to 75,58 kPa and the percentage increase in BCR was 39,90%. Meanwhile the least of soil bearing capacity occurred in soil column that had 53 cm in length and diameter of 3,2 cm while the soil bearing capacity had increased to 64,47 kPa and BCR only increased to 19,33%

A research on the inhibition of formula gel test ethanol extract of leaves of mulberry (Morus alba L.) as an anti-acne against bacteria Propionibacterium acne. This study uses a gel formulation made using mulberry leaf extract (Morus alba L.), which aims to determine the inhibition of antibacterial gel mulberry leaf extract (Morus alba L.) against the bacteria Propionibacterium acne. Gel made three dosage concentrations of 2%, 4% and 6%. Testing inhibition of antibacterial gel agar diffusion method. Test Antibacterial inhibition obtained by looking at the clear zone in the medium and measured using calipers. The results showed that the inhibition of antibacterial gel formulation with a concentration of 2%, 4% and 6% showing that inhibiting bacterial activity.