1. Dietary Supplementation of Glycyrrhetinic Acid (GA) Promoting Lipid Lipolysis from Blunt Snout Bream Megalobrama amblycephala by TNF-α and LPL Expression
- Author
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W-b. Liu, M-y Liu, M. Zhou, G-x. Liu, and G. Jiang
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Adipose tissue ,Aquatic Science ,Feed conversion ratio ,03 medical and health sciences ,chemistry.chemical_compound ,Internal medicine ,medicine ,Lipolysis ,Megalobrama ,chemistry.chemical_classification ,Lipoprotein lipase ,biology ,Cholesterol ,Fatty acid ,04 agricultural and veterinary sciences ,biology.organism_classification ,030104 developmental biology ,Endocrinology ,chemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,medicine.symptom ,Agronomy and Crop Science ,Weight gain - Abstract
Glycyrrhetinic acid (GA), which is a main active principal constituent of liquorice, is extensively used as a non-nutrition sweetener or antiinflammatory in Chinese medicine. This study was carried out to evaluate the effects of different GA levels on growth, biometric parameters, plasma lipid metabolites, Tumor necrosis factor alpha (TNF-α) and Lipoprotein lipase (LPL) gene expression of Megalobrama amblycephala (average weight, 68.78 ±1.13 g). Three diets were formulated to contain various levels of GA (0, 30 and 300 mg/kg). Fish were randomly distributed into 12 cages and fed three times daily for 8 weeks. Dietary GA levels have little significant effect on weight gain and feed conversion ratio. Intraperitoneal fat ratio and viscera/body ratio decreased significantly as dietary GA levels increased, and hepatosomatic index tended to decrease as dietary GA levels increased. Plasma triglycerides, cholesterol, and non-esterified free fatty acid levels also decreased as dietary GA concentrations increased. Adipose tissue and liver TNF-α expression increased significantly with increasing dietary GA levels; whereas, LPL expression showed an opposite trend. Results of this study indicate that dietary supplementation of 300 mg/kg GA had a positive effect in promoting lipid lipolysis in Megalobrama amblycephala without having a negative effect on growth through the regulation of TNF-α and LPL expression.
- Published
- 2018
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