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Conventional approaches for studying and molecular typing of tumors include PCR, blotting, omics, immunocytochemistry, and immunohistochemistry. The last two methods are the most used, as they enable detecting both tumor protein markers and their localizations within the cells. In this study, we have investigated a possibility of using RNA aptamers, in particular, 2′-F-pyrimidyl-RNA aptamer ME07 (48 nucleotides long), specific to the receptor of epidermal growth factor (EGFR, ErbB1, Her1), as an alternative to monoclonal antibodies for aptacytochemistry and aptahistochemistry for human glioblastoma multiforme (GBM). A specificity of binding of FAM-ME07 to the receptor on the tumor cells has been demonstrated by flow cytometry; an apparent dissociation constant for the complex of aptamer – EGFR on the cell has been determined; a number of EGFR molecules has been semi-quantitatively estimated for the tumor cell lines having different amount of EGFR: A431 (106 copies per cell), U87 (104 copies per cell), MCF7 (103 copies per cell), and ROZH, primary GBM cell culture derived from patient (104 copies per cell). According to fluorescence microscopy, FAM-ME07 interacts directly with the receptors on A431 cells, followed by its internalization into the cytoplasm and translocation to the nucleolus; this finding opens a possibility of ME07 application as an escort aptamer for a delivery of therapeutic agents into tumor cells. FAM-ME07 efficiently stains sections of GBM clinical specimens, which enables an identification of EGFR-positive clones within a heterogeneous tumor; and providing a potential for further studying animal models of GBM.

A series of 5%Ni-CexZr1−xO2 (x = 0.3, 0.5, 0.7) catalysts has been prepared via one-pot solvothermal continuous synthesis in supercritical isopropanol and incipient wetness impregnation of CexZr1−xO2 obtained by the same route. The textural, structural, red-ox, and catalytic properties in methane dry reforming (MDR) of Ni-modified Ce-Zr oxides synthesized by two routes have been compared. It was shown by XRD, TEM, and Raman spectroscopy that the method of Ni introduction does not affect the phase composition of the catalysts, but determines the dispersion of NiO. Despite a high dispersion of NiO and near-uniform distribution of Ni within Ce-Zr particles observed for the one-pot catalysts, they have shown a lower activity and stability in MDR as compared with impregnated ones. This is a result of a low Ni concentration in the surface layer due to segregation of Ce and decoration of nickel nanoparticles with support species.

The ordered mesoporous Mg- and Ni-Mg-alumina samples of general formula xwt%Mg-Al2O3 and ywt%Nixwt% Mg-Al2O3(x, y = 5, 10 % wt) have been prepared by the one-pot evaporation induced self-assembly (EISA) method using Pluronic P123 as a template. The textural and structural properties of the prepared samples have been characterized by N2adsorption-desorption analysis, BET, XRD, TEM with EDX, HAADF-STEM, and H2-TPR. The samples calcined at 700°C had an ordered mesoporous structure with a SSA of 170-250 m2/g, a pore volume of 0.5-0.64 cm3/g and a narrow distribution of the pore size (mean 12-16 nm). For the catalysts prepared by the one-pot EISA method, the size of the metal Ni particles was in the range of 3-12 nm and was conditioned by their confinement in the mesopores and the interaction with MgO and Al2O3. Testing in methane dry reforming in the 15v.% CH4+ 15v.%CO2feed, with He balance, at 650-750°C and the contact time of 0.015 s has shown the higher activity and stability of the ordered mesoporous 10w%Ni- 10w%Mg-Al2O3catalysts prepared by the one-pot method as compared with the catalyst of the same composition obtained by impregnation.

For structured catalysts of biofuels transformation into syngas inexpensive and stable to sintering active components based upon Mg-doped γ-alumina with supported LaNi 0.9 Ru 0.1 O 3 perovskite or MnCr 2 O 4 spinel promoted by Ru + Ni were developed. Characterization of the surface features, reactivity, catalytic activity and routes of ethanol transformation on these catalysts demonstrated that suppression of support acidity, strong interaction of Ru-Ni alloy nanoparticles with manganese chromite layers on support and a high redox ability provide a high yield of syngas and stability to coking. The most promising active component (2 wt% Ni + 2 wt% Ru)/MnCr 2 O 4 /10 wt% MgO − γ-Al 2 O 3 supported on microchannel heat-conducting CrAlO cermet plates demonstrated a high performance and stability to coking in the autothermal reforming of such reactive fuels as glycerol, anisol and turpentine oil.

Progress in design of thermal barrier coatings (TBCs) is based upon application of new materials and deposition techniques. In this work traditional NiCrAl bond coat YSZ top coat were deposited on Ni superalloy substrate by inexpensive dry detonation spraying, while finishing layers of oxide nanocomposites (LaAlO3–La2Zr2O7, LaAlO3–LaCuAl11O19) were deposited on YSZ by slip casting. Complex oxides were prepared via Pechini route. Thin (~ 20 μm) finishing layers were deposited by slip casting of suspensions of oxides mixture in isopropanol with addition of polyvinylbutyral. Genesis of the texture, composition and real/defect structure of bulk nanocomposite materials and deposited layers after annealing in air up to 1300 °C as well as after series of thermal shocks by heating up to 1200 °C by H2–O2 burner were studied by combination of diffraction (high resolution SEM and TEM with EDX, XRD on synchrotron radiation) and spectroscopic (UV–Vis, EXAFS, laser-excited Dy3 + luminescence spectra) methods. Thermal conductivity of nanocomposites and TBCs was determined by using NETZSCH LFA 457 MicroFlash. Nanocomposites were shown to retain porosity as well as nanosizes of disordered domains of oxide phases even after sintering at high temperatures. A good adhesion and compatibility of all layers in TBCs were demonstrated, while in general disordering of the oxides structure in deposited layers was higher than that in bulk materials due to the effect of depositing procedure and interaction between layers. This provides a low thermal conductivity of nanocomposites and functionally graded TBC. After 90 thermal shocks neither layers spallation nor cracks were revealed, 8YSZ and finishing layers retaining porosity, nanocrystallinity and disordering required for a low thermal conductivity and cracks trapping.

The sorption capacity of synthetic montmorillonites of the composition Na2x (Al2(1–x),Mg2x )Si4O10(OH)2 · nH2O (where 0 < x < 1) in relation to the methylene blue dye has been investigated. The obtained results from the data for natural samples of montmorillonite (K10) and activated carbon have been compared. The effect of the montmorillonite composition and the medium acidity on the degree of dye adsorption has been studied. The chemical composition of montmorillonite, which is optimal for solving tasks in the field of ecology and medicine, has been determined. The character of the interaction of montmorillonites with the adsorbed substance from the point of view of the physical-chemical sorption models has also been studied.

Modelling experiments on vitamin B1 (thiamine hydrochloride) intercalation into synthetic montmorillonite (Mt) structures of the systematically varied compositions Na2x(Al2(1–x),Mg2x)Si4O10(OH)2 ∙ nH2O (where 0

High-dose exposure of mice to perfluorooctanoate (PFOA) induces both hepatotoxicity and immunotoxicity. Here, we characterized the effects of 10-day dietary treatment with PFOA (0.002-0.02%, w/w) on the liver and complement system of male C57BL/6 mice. At all four doses, this compound caused hepatomegaly and reduced the serum level of triglycerides (an indicator for activation of the peroxisome proliferator-activated receptor-alpha (PPARα)). At the highest dose (0.02%, w/w), this hepatomegaly was associated with the hepatic injury, as reflected in increased activity of alanine aminotranferase (ALAT) in the serum, severe hepatocyte hypertrophy and hepatocellular necrosis. PFOA-induced hepatic injury was associated with in vivo activation of the complement system as indicated by (i) significant attenuation of the serum activities of both the classical and alternative pathways; (ii) a marked reduction in the serum level of the complement factor C3; and (iii) deposition of the complement factor C3 fragment (C3a) in the hepatic parenchyma. PFOA did not activate the alternative pathway of complement in vitro. At doses lower than 0.02%, PFOA induced hepatocyte hypertrophy without causing liver injury or activating complement. These results reveal substantial involvement of activation of complement in the pathogenesis of PFOA-induced hepatotoxicity.

The study of the sorption capacity of synthetic layered silicates with a montmorillonite structure towards thiamine hydrochloride was performed. The influence of the pH of the medium and the composition of montmorillonite on the thiamine adsorption degree was studied. The optimum chemical composition of montmorillonite was determined, which makes it possible to carry out an efficient intercalation of thiamine in a wide pH range.

INGENIERIE+ABB:YSC:CMI; The mechanism of CH4 dry reforming on Pt, Ru, Ni, Ni + Ru-supported perovskite (PrFeOx, LaPrMnCrOx) or fluorite (LnCeZrO(x)) oxides was studied using a Setaram Sensys DSC TG calorimeter and a pulse kinetic installation. For catalysts in the steady-state, CH4 and CO2 transformation in separate pulses proceeds with the rate and products selectivity equal to that in mixed CO2 + CH4 pulses. Heat effects of separate stages correspond to CH4 oxidation into syngas by strongly bound bridging oxygen forms of support (heat of adsorption up to 650 kJ mol(-1) O-2 for fluorites and similar to 500 kJ mol(-1) O-2 for perovskites) and their replenishment by CO2 dissociation, respectively. These features demonstrate a step-wise red-ox (Marsvan-Crevelen) mechanism of CH4 dry reforming. Fast oxygen transfer from the sites of oxide support to the metal/oxide interface provides required conjugation of stages. (C) 2013 Elsevier B.V. All rights reserved.

The development of immunodeficiency in HIV-infected patients is known to result from CD4+ lymphocyte depletion. Most CD4+ lymphocyte cells destined to die are not infected. The mechanism of HIV-uninfected cell death has not yet been fully elucidated. The aim of this study is to examine the role of anti-CD4 autoantibodies and physiological rheumatoid factor (RF) in the development of CD4+ lymphocytopenia. Immunization of Wistar rats with gp120 HIV-1 induces chronic production of anti-CD4 autoantibodies and decreases CD4+ lymphocytes in the blood. However, the anti-CD4 autoantibodies produced as part of the immune response to gp120 do not kill CD4+ cells directly. In rats producing anti-CD4 autoantibodies, a low level of peripheral CD4 lymphocytes is associated with high blood RF levels. The sera containing RF killed lymphocytes when the lymphocytes were pretreated with sera containing anti-CD4 autoantibodies. Thus, the death of CD4+ lymphocytes in rats immunized with gp120 is a result of the combined action of anti-CD4 autoantibodies and RF, and the action of these factors can be separated in time. The fact that two signals are needed for CD4+ lymphocyte death in HIV gp120-immunized rats does not contradict the hypothesis of the activation-induced death of uninfected CD4+ cells in HIV-infected humans.

Apatite type rare earth silicates are being extensively studied as electrolyte material for intermediate temperature solid oxide fuel cells (SOFC). In this paper we presents results on synthesis of Al and/or Fe-doped ATLS, the design of compatible anode materials, thermal expansion properties and co-sintering of half-cells from expansion matched materials using the advanced pulsed electric current sintering (PECS) technique. The issues related to the co-sintering of half cells have been addressed successfully by the combined use of nano powders and PECS.

The present paper is devoted to optimization of composites (100-z) wt.% La1-ySryFe0.7Ni0.3O3-δ - z wt.% Ce0.9Gd0.1O2-δ (GDC) and study of the effect of Sr doping of perovskite and GDC content in the sample on structural and transport features of the composites. The results on the research of structural and microstructural features of the composites and their transport properties by using XRD, HRTEM, EDX analysis, electrical conductivity measurements, oxygen isotope heteroexchange and conductivity as well as weight relaxation techniques are presented and discussed.

Apatite-type lanthanum silicates (ATLS) doped by Fe were prepared by using mechanical activation, and the effect of the nature of the dopant raw materials on the formation of ATLS was considered. Genesis of the formation of apatite was studied by XRD, IR, HRTEM, and Mossbauer and UV/ Vis electron spectroscopy. The possibility of a partial substitution of Si in the apatite with a dopant and the mechanism of formation of ATLS in the course of mechanical activation depend on the dopant parent compound. The structural compatibility of La2O3, La(OH)3, and apatite was shown to favor

Structural features of Fe- and Al-doped apatite-type lanthanum silicates with different stoichiometry have been studied by using XRD, XPS, IR, UV-Vis, EXAFS, Mossbauer, 29Si and 27Al MAS NMR spectroscopy. The transport properties of the samples were also characterized by using impedance spectroscopy and oxygen isotope heteroexchange.

Catalytic properties and coking stability of composite materials based on NiO and doped apatite-type lanthanum silicates were studied for their using as anode materials for internal methane reforming in intermediate temperature solid oxide fuel cells. Samples based on Sr- or Al-doped lanthanum silicate were prepared by modified Pechini methods. The composite materials were characterized by X-ray diffraction, methane steam reforming and temperature programmed oxidation. The effects of La 0.8 Sr 0.2 Mn 0.8 Cr 0.2 O 3 addition, partial Ni substitution with La 0.1 Sr 0.9 TiO 3 ionic and electronic mixed conductor and composition of apatite-type lanthanum silicate on catalytic properties and coking resistance were considered.

A number of catalysts based on Al 2 O 3 loaded with doped Ce-Zr mixed oxides and different active components (Cu, Cu-Ni, Ru, Pt, etc.) were synthesized via standard wet impregnation method using the robotic workstation. Ethanol (EtOH) was taken as a model compound of bio-oil and steam reforming of ethanol (ESR)—as a model reaction. Activity screening experiments performed at 600–700 °C in 0.5 vol.% C 2 H 5 OH + 2.5 vol.% H 2 O + 97 vol.% He mixture revealed that the most effective catalyst composition is Ru/Ce 0.4 Zr 0.4 Sm 0.2 /Al 2 O 3 . Catalytic activity investigations at high reagent concentrations (10 vol.% C 2 H 5 OH + 40 vol.% H 2 O + 50 vol.% N 2 ) at 650–800 °C confirmed this fact, revealing also that at high temperatures the activity of Cu-Ni catalysts is comparable with that of Ru-containing catalyst.

Al-doped La9.33+x/3+ySi6–xAlxO26+3y/2 (x = 0–1.5, y = 0–0.67) apatite-type lanthanum silicates (ATLS) were synthesized with a high-power (1200 rpm) planetary ball mill that allows the formation of the lanthanum silicate phase after milling for 20–35 min at room temperature. The apatite phase already appears after milling for 5 min. Phase, structural and microstructural studies on the nanocrystalline lanthanum silicates were carried out by using XRD, TEM, and IR, 27Al and 29Si MAS NMR spectroscopy to clarify the mechanism of ATLS formation. The cluster-topotactic mechanism of the formation of apatite by mechanochemical treatment involving hydration of the starting compounds and acid–base reactions is suggested. Al-doping results in promoting the formation of apatite. However, a secondary LaAlO3 phase may be present in the sample depending on its stoichiometry. This suggests the existence of a biphase domain in the La2O3–SiO2–Al2O3 phase diagram at room temperature, in which Al-doped apatite and LaAlO3 phases coexist. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)

Al- and Sr-doped apatite-type lanthanum silicates (ATLS) have been prepared using mechanochemical activation (MA) and Pechini (Pe) methods. MA of a stoichiometric mixture in the high-power planetary ball mill provides the formation of a high purity crystalline ATLS at room temperature after 20–35 min activation. The phase composition and local structure of doped ATLS are determined by milling time and the dopant content. 5 min MA of carbonate precursors obtained via Pe method results in the formation of a single-phase ATLS at 900°C.

Advancement in fluid diverter technology has enabled successful initial stimulation of long interval carbonates without mechanical isolation, in excess of 1000 ft. by using in-situ crosslinked polymer systems and viscoelastic surfactant systems. These fluids build viscosity upon reacting with the formation, enhancing diverter performance. However, heavily stimulated, i.e. wormholed, formations require a step-level change in diversion performance to effectively stimulation new intervals, or previously under-stimulated layers. This paper will present the qualification and optimization of degradable fibers for use in re-stimulation of carbonate formations. The key performance aspects desired and evaluated are: (1) ability to block existing wormholes near the wellbore, as opposed to needing to fill the entire wormhole structure, (2) permeability and diversion capability of resulting fiber bridge, and (3) confirmation of degradation and cleanup in a dry gas environment within a reasonable timeframe. New experimental methods were developed to address these requirements, including gas/fluid degradation testing, bridging tests with slots to represent wormholes and fractures, and bridging experiments with 3D synthetic models of a wormhole structure. To be able to run repeatable experiments, wormholed structures were printed with a 3D printer using high-resolution CT scans from an actual wormholed core plug. Testing results showed the ability of the fibers to bridge within the wormhole network and provided guidance in designing the fiber diverter stages for already-stimulated wells. Addition of degradable particulates was shown to further improve wormhole bridging and diversion performance. Modeling the wormhole diversion process within advanced stimulation software identified additional optimizations, such as increased stage size and increased fiber concentrations to achieve desired diversion. A related presentation will discuss the successful re-stimulation of three high-rate gas wells (B. Clancey 2015).

LnFeNi(Ru)O3 perovskites synthesized via a modified Pechini method have been studied as catalyst precursors in CO2 reforming of methane. The structural and redox properties along with catalytic performance were found to depend upon Ln nature and Ru content in the perovskite. Active and stable catalysts have been formed from perovskite precursors in reaction media at high temperatures due to Fe–Ni–(Ru) alloy particles segregation from the perovskite lattice and their stabilization on the oxide surface. Coking stability of these catalysts in dry reforming of real natural gas containing admixtures of higher hydrocarbons is explained by combined effects of Ni dilution by Ru in alloy nanoparticles and high mobility and reactivity of surface/lattice oxygen in the disordered Ln ferrite support.

Recently, a new fiber-laden, self-diverting, and viscoelastic acid has been successfully used for matrix acidizing of highly heterogeneous carbonate formations The fibers have been designed to be inert under surface and pumping conditions, and their geometry allows them to form strong and stable fiber networks that can effectively bridge across natural fractures, wormholes, and perforation tunnels. Eventually, the fibers degrade into a water-soluble organic liquid that is produced back to the surface during flowback. In the case of perforated wells, experiments suggest that diversion with fibers operates in three phases. First, as the early volumes of fiber-laden acid reach the perforations, the acid penetrates the reservoir as if no fibers were present. Second, as the fibers bridge, they accumulate inside the perforations and form a fiber cake. Third, the fibers plug the perforation, and the injectivity decreases locally, promoting diversion into other perforations. The pressure drop through a plugged perforation was analyzed by performing 340 separate fine-scale 3D simulations. The original work was based on theoretical and laboratory-based experiments, considering typical perforation schemes and for various permeability ratios between the generated fiber cake and the formation's original permeability. The results were compiled, and a correlation was made to model the resulting skin. The model was implemented into an acid placement simulator and was extensively tested and validated in the field. In this paper, we present the model that describes the effect of fiber accumulation within perforations and explain how some of the model parameters such as formation-permeability contrast, fiber-cake permeability, and total permeability thickness (kh) may affect diversion efficiency. Case studies from field testing of the model illustrate methods of pressure history matching, job design, and treatment history evaluation.

Structured catalysts based on metallic supports of different types with a high thermal conductivity were designed. The metal monolithic supports were made from a heat-resistant foil or gauze coated with a protective layer of Al 2 O 3 or zrO 2 using blast dusting. To prepare catalysts, Ce-Zr-La-O and LaNiO x promoted with Pt were successively supported on monoliths. A high and stable performance of these catalysts in the partial oxidation of methane (POM) at short contact times in the autothermal mode was demonstrated. In the reactor with a heat exchanger these catalysts operate in the autothermal mode without any heat preheat at very high gas velocities.

Composite materials based on NiO and doped apatite-type lanthanum silicates (ATLS) that are new class of solid electrolytes were studied as anode materials for intermediate temperature solid oxide fuel cells (IT SOFC), with possibility to use both H2 and CH4 as a fuel being payed attention to. For cermets preparation different methods of Ni addition such as incipient wetness impregnation, modified Pechini methods or mechanical mixing were used. The composite materials were characterized by XRD, BET, TPO and methane steam reforming reaction. Doping with complex oxides possessing a high mobility of the lattice oxygen and partial Ni substitution by the mixed conductor were applied to improve anode stability to coking.

Structural features and transport properties of Fe- and Al-doped apatite-type lanthanum silicates were studied with relation to a role of the [SiO4] substructure. XRD, TEM, Mössbauer, UV-Vis electron, IR, 29Si and 27Al NMR spectroscopy were used for structural studies; impedance spectroscopy and oxygen isotope heteroexchange were used for transport properties characterization.