9 results on '"Samir Ibenmoussa"'
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2. Comparative study of the chemical composition, antioxidant, and antimicrobial activity of the essential oils extracted from Lavandula abrialis and Lavandula stoechas: in vitro and in silico analysis
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Mohamed Radi, Zaina Eddardar, Aziz Drioiche, Firdaous Remok, Md. Eram Hosen, Khalid Zibouh, Brahim Ed-Damsyry, Amale Bouatkiout, Sanae Amine, Hanane Touijer, Ahmad Mohammad Salamatullah, Mohammed Bourhia, Samir Ibenmoussa, and Touriya Zair
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lavender ,quality control ,phytochemicals ,antioxidant/antimicrobial activities ,oulmès -Morocco ,molecular docking ,Chemistry ,QD1-999 - Abstract
This work aims to add value to the Lavandula genus by identifying the chemical composition, antioxidant, and antimicrobial activities of two species lavender from Oulmès in Morocco; Lavandula abrialis and Lavandula stoechas. The uniqueness lies in the integrated approach that combines in vitro and in silico analyses to assess the biological properties of the essential oils (EO). The objective of this study is to enhance the significance of the Lavandula genus by analyzing the chemical composition, antioxidant properties, and antimicrobial effects of two lavender species found in Oulmès, Morocco: Lavandula abrialis and Lavandula stoechas. The distinctiveness is in the comprehensive methodology that merges in vitro and in silico investigations to evaluate the biological characteristics of the essential oils (EO). The extraction of essential oils (EO) by hydrodistillation from the aerial parts of Lavandula abrialis gave a high yield of essential oils (2.9%) compared to Lavandula stoechas (2.3%). A GC-MS analysis of the chemical composition revealed 56 chemical compounds, with some variation in the predominant components, representing between 99.98% and 100% of the EOs of the studied lavenders. Their antioxidant activity was assessed using the DPPH test. This method revealed that L. stoechas EO has a higher percentage of free radical inhibition than L. abrialis. The IC50 values demonstrate that the antioxidant activity of ascorbic acid is higher (1.62 g/mL) than the EOs of tested plants. Noteworthy, the EO of L. stoechas is more potent (12.94 g/mL) than that of Lavandula tibialis (34.71 g/mL). Regrading, the antibacterial tests, the EO of L. abrialis was particularly active against Staphylococcus aureus BLACT, which is inhibited at a concentration of 6.25 g/mL, while L. stoechas EO has a strong effect on Escherichia coli, with a MIC of 1.56 g/mL. Concerning the antifungal activity of the EOs, yeasts showed sensitivity toward EOs extracted from both L. tibialis and L. stoechas. Moreover, an in silico study was conducted targeting sarA protein of S. aureus (PDB ID: 2fnp) and NADPH oxidase from Lavandula sanfranciscensis (PDB: 2CDU) and results showed that Ishwarone and Selina-3,7 (11)-diene exhibited highest binding energy with −9.8 and −10.8 kcal/mol respectively. Therefore, these two compounds could be used as an antibacterial and antioxidant agents however more experimental and molecular study should be required.
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- 2024
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3. Isolation of Arborescin from Artemisia absinthium L. and Study of Its Antioxidant and Antimicrobial Potential by Use of In Vitro and In Silico Approaches
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Asmae Hbika, Amine Elbouzidi, Mohamed Taibi, Safae Ouahabi, El Hassania Loukili, Abdelhamid Bouyanzer, Meryem Idrissi Yahyaoui, Abdeslam Asehraou, Naoufal El Hachlafi, Ahmad Mohammad Salamatullah, Mohammed Bourhia, Samir Ibenmoussa, Mohamed Addi, and Elkhadir Gharibi
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arborescin ,crystal structure ,recrystallization ,Artemisia absinthium ,antimicrobial ,antioxidant ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
This study focused on developing an innovative, straightforward, and economical method utilizing a mixture of readily available solvents to extract arborescin (C2OH2OO8) crystals from Artemisia absinthium L. (A. absinthium). The structural elucidation and characterization were conducted using a suite of techniques including IR spectroscopy, CNHSO elemental analysis, scanning electron microscopy and energy dispersive X-ray spectroscopy (SEM-EDS), and mass spectroscopy (MS). Density functional theory (DFT) calculations were employed to determine the molecular properties. Antioxidant activity was measured using the DPPH radical scavenging assay and the β-carotene bleaching test. Antimicrobial efficacy was assessed against four bacterial strains and three fungal strains. The molecular docking approach was employed to predict the probable binding patterns and affinities of arborescin with specific target biomolecules. Employing an array of analytical techniques, examination of the isolated crystal from A. absinthium. led to its comprehensive structural elucidation. IR spectroscopy revealed the presence of distinctive functional groups, including a carbonyl group within the γ-lactone and an epoxy group. CNHSO elemental analysis verified that the crystal contained only carbon, hydrogen, and oxygen, a finding corroborated by SEM-EDS analysis, consistent with the molecular structure of arborescin. Additionally, mass spectrometry confirmed the identity of the compound as arborescin, with a molecular ion with a mass m/z = 248. Quantum-Chemical Descriptors revealed that arborescin is resistant to elementary decomposition under standard conditions. Although arborescin demonstrates a relatively low antioxidant capacity, with an IC50 of 5.04 ± 0.12 mg/mL in the DPPH assay, its antioxidant activity in the β-carotene bleaching test was found to be 3.64%. Remarkably, arborescin effectively inhibits the growth of Staphylococcus aureus and Listeria innocua at low concentrations (MIC = 166 µg/mL). Additionally, it exhibits significant antifungal activity against Candida glabrata, with a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of 83 µg/mL and 166 µg/mL, respectively. In this study, arborescin exhibited a robust docking score of −8.1 kcal/mol, indicating a higher affinity compared to ciprofloxacin. This suggests that arborescin has significant potential as a potent antibacterial agent.
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- 2024
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4. Chemical composition, antioxidant, and antimicrobial properties of Mentha subtomentella: in sight in vitro and in silico analysis
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Fatima Brahmi, Nour Eddine Bentouhami, Youssef Rbah, Amine Elbouzidi, Ouafae Mokhtari, Ahmad Mohammad Salamatullah, Samir Ibenmoussa, Mohammed Bourhia, Mohamed Addi, Abdeslam Asehraou, and Bouchra Legssyer
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M. subtomentella ,essential oil ,aqueous extract ,GC/MS ,HPLC/DAD ,antimicrobial activity ,Chemistry ,QD1-999 - Abstract
Our research focused on assessing essential oils (MSEO) and aqueous extracts (MSAE) derived from M. subtomentella leaves, with a primary focus on evaluating their properties. From 1 kg of leaves, we successfully obtained 18 mL of essential oil. Upon conducting GC/MS analysis, we identified eleven compounds within the oil, collectively accounting for 100% of the constituents identified. Notably, the predominant compounds in the leaf oil were p-Menth-48) -en-3-one (50.48%), 9-Ethylbicyclo (3.3.1) nonan-9-ol (10.04%) (E)-3,3-Dimethyl-delta-1, alpha-cyclohexaneacetaldehyde (8.53%), and D-Limonene (7.22%). Furthermore, utilizing HPLC/DAD, we explored the phenolic profile of MSAE, extracted through decoction. This analysis revealed the presence of fifty-eight compounds, with five major components collectively constituting 61% of the total compounds identified, rosmarinic acid as the major one. We evaluated the antimicrobial effectiveness of the MSEO against ten different strains, observing its notable efficacy against A. Niger (MIC = 0.09%), P. digitatum (MIC = 0.5%), and G. candidum (MIC = 1%). However, the essential oil demonstrated comparatively lower efficacy against bacteria than fungi. In contrast, the MSAE did not exhibit any antimicrobial activity against the tested strains. Regarding antioxidant activity, the aqueous extract displayed a significantly higher antioxidant capacity than the essential oil, which exhibited relatively lower antioxidant activity. The IC50 values were determined to be 0.04 ± 0.01 mg/mL, 0.17 ± 0.01 mg/mL, and 13% ± 0.01% (V/V), for ascorbic acid MSAE and MSEO, respectively. We used a computational method called molecular docking to investigate how certain plant compounds affect antioxidant, antibacterial, and antifungal activities. This involved analyzing the interactions between these compounds and specific protein targets known for their roles in these activities.
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- 2024
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5. Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases
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Samir Ibenmoussa, Mohammed Bourhia, Riaz Ullah, Hafiz Majid Mahmood, Admou Brahim, Youssef El Hassouni, Syed Saeed Ali, and Ahmed Bari
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030203 arthritis & rheumatology ,Chemistry ,immunodot ,Autoantibody ,General Chemistry ,antinuclear antibodies ,indirect immunofluorescence ,03 medical and health sciences ,0302 clinical medicine ,systemic autoimmune diseases ,immunoblot ,030220 oncology & carcinogenesis ,Immunology ,Materials Chemistry ,In patient ,QD1-999 - Abstract
Autoimmune diseases are pathological conditions in which the immune system mistakenly attacks its own tissues. This study evaluates the performance of two techniques, which are identifiers of autoantibody specifics: immunoblot and immunodot. This study was conducted in 300 patients of whom 62 were tested positive for antinuclear antibodies. The patients were initially screened for antinuclear antibodies using indirect immunofluorescence. Then, the identification of specific autoantibodies such as anti-extractable nuclear antigens (ENAs) was carried out using the immunoblot and immunodot techniques. The results showed that immunoblot and immunodot did not present a significant difference in their sensitivity against anti-SSA/52, SSB, CENP-B, PCNA, U1-snRNP, Jo-1, Pm-scl, and Mi-2 (p > 0.05). However, the two techniques showed a significant difference in their sensitivity toward autoantibodies anti-DNAn, anti-histone, anti-SmD1, and anti-ds-DNA (p < 0.05). The immunoblot data were in complete accordance with the immunodot data (100%) regarding the detection of autoantibodies such as anti SSA/52, SSB, CENP-B, PCNA, U1-snRP, Jo-1, Pm-scl, and Mi-2, 80% regarding SmD1, and 75% concerning ds-DNA. We should certainly pay closer attention to the efficiency of the techniques used in the diagnosis of autoimmune diseases.
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- 2021
6. Physicochemical evaluation of the fruit pulp of Opuntia spp growing in the Mediterranean area under hard climate conditions
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Abdelaaty A. Shahat, Samir Ibenmoussa, Mohammed Bourhia, Hamza Elmahdaoui, and Riaz Ullah
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010405 organic chemistry ,Chemistry ,opuntia ficus-indica ,Pulp (paper) ,biochemical characterization ,010401 analytical chemistry ,food and beverages ,General Chemistry ,engineering.material ,01 natural sciences ,0104 chemical sciences ,variety ,opuntia megacantha ,Horticulture ,physicochemical characterization ,Materials Chemistry ,engineering ,Mediterranean area ,prickly pear ,QD1-999 - Abstract
Barbary fig called prickly pear is a plant belonging to family Cactaceae growing under hard climate conditions. A spiny variety of prickly pear named “Drbana” (Opuntia megacantha) and two non-spiny varieties named “Akria” and “Mlez” (Opuntia ficus-indica) growing in the Rhamna region (Morocco) were studied in terms of physicochemical characteristics. The physicochemical characterization (humidity, water activity, pH, total titratable acidity, Brix, and ash content) and the biochemical characterization (total carotenoid content, betalain content, total polyphenolic content, and ascorbic acid content) of the fruit pulp of prickly pear were performed according to the previously reported methods. The finding of physicochemical characterization of all studied varieties showed that the fruit pulp also contained an interesting bioactive compound classes in humidity, water activity, pH, total titratable acidity, Brix, and ash content. Regarding the biochemical characterization, the obtained finding showed the fruit pulp also contained an interesting bioactive compound classes particularly the total betalains, polyphenols, carotenoids, and ascorbic acids. Based on the obtained results in the current research work, we can affirm that the fruits of all studied varieties meet the requirement for being exploited in food, cosmetic, and pharmaceutical industries.
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- 2020
7. Physicochemical Evaluation of Edible Cyanobacterium Arthrospira platensis Collected from the South Atlantic Coast of Morocco: A Promising Source of Dietary Supplements
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Riaz Ullah, Mounia Cherki, Touria Ould Bellahcen, Samir Ibenmoussa, Ahmad Mohammad Salamatullah, Naima Khlil, Hanane Ennaji, Ikram Taouam, Heba Khalil Alyahya, Aziz Falaq, Mohammed Bourhia, and Abdulhakeem Alzahrani
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chemistry.chemical_classification ,Chlorophyll b ,0303 health sciences ,Cadmium ,Chlorophyll a ,Article Subject ,030309 nutrition & dietetics ,chemistry.chemical_element ,Heavy metals ,Other systems of medicine ,03 medical and health sciences ,chemistry.chemical_compound ,Pigment ,Complementary and alternative medicine ,chemistry ,visual_art ,Food supply ,visual_art.visual_art_medium ,Arthrospira platensis ,Food science ,Carotenoid ,RZ201-999 ,030304 developmental biology ,Research Article - Abstract
The cyanobacterium Arthrospira platensis (A. platensis)—a genus of nonheterocystous filamentous cyanobacteria—is used in industrial applications and as a food supply. The current research work aims to study the physicochemical characteristics of A. platensis indigenous to the Moroccan Atlantic coast at Laayoune (Foum El Oued lagoon). The contents of proteins, carbohydrates, vitamins, lipids, minerals, heavy metals, energy value, humidity, ash, pigments, and tannins in A. platensis were investigated using protocols as described in the earlier literature. The values of protein, carbohydrate, and lipid contents in A. platensis were 58.9 ± 0.07, 14.67, and 45.54% respectively. The values of vitamins B2 and B3 dosed in A. platensis were 1.31 ± 0.19 and 30.8 ± 0.001 mg/kg, respectively. The values of heavy metals including lead and chromium were 70 ± 4.5 and 5 ± 0.5 PPB (parts-per-billion), respectively; however, no trace concerning cadmium was detected. The values of energy value, humidity, and ash content were 346.48 ± 0.21, 11.6 ± 0.17%, and 9.1 ± 0.21% kcal/100 g, respectively. The results of pigment content showed the presence of chlorophyll b, chlorophyll a, and carotenoids of 37.506 ± 3.38, 26.066 ± 3.08, and 9.52 ± 0.22 mg/g, respectively. The results obtained revealed that A. platensis indigenous to the Moroccan Atlantic coast at Laayoune was found to be very rich in proteins, carbohydrates, vitamins, minerals, ash, and pigments and lower in heavy metals and saturated fats when compared with species investigated in the literature. Thus, A. platensis indigenous to the Moroccan Atlantic coast at Laayoune fulfills the requirements for being used as dietary supplements.
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- 2021
8. Caralluma europaea (Guss.) N.E.Br.: Anti-Inflammatory, Antifungal, and Antibacterial Activities against Nosocomial Antibiotic-Resistant Microbes of Chemically Characterized Fractions
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Riaz Ullah, Essam Ezzeldin, Samir Ibenmoussa, Gamal A. E. Mostafa, Ahmed Bari, Andriy Grafov, Meryem Slighoua, Mohammed Bourhia, Dalila Bousta, Fatima Ez-Zahra Amrati, Hamza Saghrouchni, and Department of Chemistry
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Antifungal ,medicine.drug_class ,116 Chemical sciences ,Pharmaceutical Science ,) N ,01 natural sciences ,Article ,Anti-inflammatory ,Analytical Chemistry ,lcsh:QD241-441 ,03 medical and health sciences ,Antibiotic resistance ,Caralluma europaea (Guss ,lcsh:Organic chemistry ,Drug Discovery ,medicine ,Caralluma europaea (Guss.) N.E.Br ,Physical and Theoretical Chemistry ,030304 developmental biology ,anti-inflammatory ,2. Zero hunger ,0303 health sciences ,Caralluma europaea ,Traditional medicine ,Apocynaceae ,biology ,chemical characterization ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,food and beverages ,biology.organism_classification ,0104 chemical sciences ,antibacterial ,Chemistry (miscellaneous) ,Polyphenol ,Molecular Medicine ,Br ,Bacteria ,antifungal ,Paw edema - Abstract
Caralluma europaea (Guss.) N.E.Br.: (C. europaea) is a wild medicinal plant belonging to the family Apocynaceae. It is commonly used in traditional medicines for treating several diseases. The present work aims to evaluate the anti-inflammatory, antibacterial, and antifungal potentials of C. europaea fractions including hydro ethanol (ET CE), n-butanol (But CE), and polyphenol (Poly CE). The chemical composition of hydroethanol, n-butanol, and polyphenol-rich fractions from C. europaea were determined using GC-MS after silylation. The anti-inflammatory effect of hydroethanol, n-butanol, and polyphenol-rich fractions was studied by carrageenan-induced paw edema. Antibacterial and antifungal activities of hydroethanol, n-butanol, and polyphenol-rich fractions against Gram-positive bacteria, Gram-negative bacteria, and yeasts were assessed using the disc diffusion and micro-dilution assays. The findings of the chemical characterization affirmed the presence of interesting bioactive compounds in C. europaea fractions. The polyphenol-rich fraction was the best inhibitor of edema by75.68% after 6 h of treatment. The hydroethanol fraction was the most active against both bacteria and yeasts. This study contributes to society as it provides potential bioactive compounds in C. europaea extract, which may help in fighting nosocomial antibiotic-resistant microbes.
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- 2021
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9. Anacyclus pyrethrum (L) : Chemical Composition, Analgesic, Anti-Inflammatory, and Wound Healing Properties
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Mohammed Bourhia, Imane Es-Safi, Riaz Ullah, Gamal A. E. Mostafa, Andriy Grafov, Hamada Imtara, Abdelfattah El Moussaoui, Amina Bari, Dalila Bousta, Hamza Mechchate, Fatima Zahra Jawhari, Essam Ezzeldin, Samir Ibenmoussa, and Department of Chemistry
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Anacyclus pyrethrum (L) ,Pyrethrum ,116 Chemical sciences ,Pharmaceutical Science ,wound healing ,01 natural sciences ,Analytical Chemistry ,Palmitic acid ,chemistry.chemical_compound ,0302 clinical medicine ,LEVULINIC ACID ,ANTIOXIDANT ,PELLITORINE ,Drug Discovery ,anti-inflammatory ,2. Zero hunger ,Traditional medicine ,biology ,EXTRACT ,analgesic ,3. Good health ,Phytochemical ,Chemistry (miscellaneous) ,317 Pharmacy ,Molecular Medicine ,Anacyclus pyrethrum ,medicine.drug_class ,Malonic acid ,Anti-inflammatory ,Article ,lcsh:QD241-441 ,03 medical and health sciences ,Acetic acid ,lcsh:Organic chemistry ,ROOT ,medicine ,LEAVES ,Physical and Theoretical Chemistry ,GC-MS analysis ,Organic Chemistry ,IN-VITRO ,biology.organism_classification ,0104 chemical sciences ,CINNAMON ,010404 medicinal & biomolecular chemistry ,chemistry ,GALANGA ,Wound healing ,030217 neurology & neurosurgery - Abstract
Background: Anacyclus pyrethrum (A. pyrethrum) is a wild species belonging to the family Asteraceae, which is used in traditional medicines. Aim of the study: This work was undertaken to study the chemical composition, analgesic, anti-inflammatory, and wound healing properties of hydroalcoholic extracts of different parts (roots, seeds, leaves, and capitula) of A. pyrethrum. Material and Methods: The phytochemical analysis of the studied extracts was conducted by GC-MS. The analgesic activity was evaluated in mice using acetic acid and formaldehyde methods. The anti-inflammatory activity was tested using the inhibitory method of edema induced in rats. The healing activity of the hydroethanolic extracts was explored by excision and incision wound healing models in rats. Results: The phytochemical analysis of the studied plant extracts affirmed the presence of interesting compounds, including some newly detected elements, such as sarcosine, N-(trifluoroacetyl)-butyl ester, levulinic acid, malonic acid, palmitic acid, morphinan-6-One, 4,5.alpha.-epoxy-3-hydroxy-17-methyl, 2,4-undecadiene-8,10-diyne-N-tyramide, and isovaleric acid. The extracts of different parts (roots, seeds, leaves, and capitula) exhibited promising anti-inflammatory, analgesic, and wound healing effects, with percentages of inhibition up to 98%, 94%, and 100%, respectively. Conclusion: This study might contribute towards the well-being of society as it provides evidence on the potential analgesic, anti-inflammatory, and wound healing properties of A. pyrethrum.
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- 2020
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