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Changes of the addition of poultry litter (PL) and poultry litter biochar (PLB) on quantitative and qualitative humus parameters in loamy sand were estimated during the 5-year study period. The following properties were determined in soil: pH, total carbon (Ctotal), total nitrogen (Ntotal), humic and fulvic acids, extracted carbon, and non-hydrolysing carbon. Additionally, light absorbance in the solutions of humic acids was computed at the wavelength of 280, 465, and 665 nm. It was demonstrated that organic matter mineralisation was most intense in soil with the addition of PL, causing significant quantitative and qualitative changes in humus compounds in soil. A slower rate of organic matter mineralisation was observed in soil amended with PLB, especially in a dose of 5.0 t ha−1, which indicated the long-term effect of this material on improving soil properties. Spectrophotometric indexes for the solution of humic acids also showed that PLB had a more favourable effect on the structure durability and lower mobility of humic acid carbon compared with PL. The application of PL and PLB significantly increased the non-hydrolysing carbon content in soil, indicating greater stabilisation of humus compounds and, at the same time, lower CO2 emissions. It was found that the addition of organic materials to soil significantly increased the soil organic carbon contents. Our study has shown that the identification of changes that may occur in the quantitative and qualitative composition of soil humus after the application of PLB may be helpful in determining the appropriate biochar dose.

New ways of managing hop sediments from brewing and fermentation of beer are constantly being sought and the existing ones improved as part of the circular economy. These wastes are not generated in large amounts, but the absence of their proper characteristics may lead to further processing errors. The study analyses the composting of hop waste under laboratory conditions and the effects of the biological activity of composts introduced into the soil. The study was carried out on two-hop sediments from two different technological stages of beer production: hot trub (HT) and spent hops (SH). Chemical, microbiological and biochemical analyses, as well as the assessment of phytotoxicity to Lepidium sativum L., were performed in the materials. The results proved that, due to their formation technology, the tested feedstocks are safe from an epidemiological point of view and are not a source of microbial contamination. Inhibitory properties for plant development were found for HT and it most likely results from the organic compound content. The mineral composition of hot trub does not exclude composting if its share in biomass is low. Spent hops (SH) were characterised by a significant total nitrogen content, which affected the composting process. Composting this sediment required the selection of substrates with a wide C:N ratio. The number of microorganisms inhabiting the analysed material was very diverse and varied depending on the type of sediments. The sample that contained the composted SH from dry hopping had a much larger number of microorganisms than the sample with HT from wet hopping.

A recently reported dual LPA1/LPA3 receptor antagonist (1) has been modified so as to modulate the basicity, sterics, and dipole moment of the 2-pyridyl moiety. Additionally, the implications of installing nonhydrolyzable phosphate head group isosteres with regard to antagonist potency and selectivity at LPA receptors is described. This study has resulted in the development of the first nonhydrolyzable and presumably phosphatase-resistant LPA3-selective antagonist reported to date.

A recently reported dual LPA1/LPA3 receptor antagonist (VPC12249, 1) has been modified herein so as to optimize potency and selectivity at LPA receptors. Compounds containing variation in the acyl lipid chain and linker region have been synthesized and screened for activity at individual LPA receptors. LPA1-selective (14b) and LPA3-selective (10g,m) compounds of modest potency have been discovered. Additionally, 2-pyridyl derivative 10t exhibits a Ki value of 18 nM at the LPA1 receptor and is significantly more potent than 1 at the LPA3 receptor. This paper describes the synthetic methods, biological evaluation, and structure-activity relationships (SARs) of LPA receptor antagonists.

Mild trypsin proteolysis (enzyme concentration 0.05% at 15°C, pH 7.4; mixing 30 min at 20 rpm) of bream mince improved mince susceptibility to freeze texturization. Formation of porous-fibrous texture of heat-set, freeze-texturized minces was associated with strong fragmentation of myosin heavy chain (MHC), decrease in extractability of myofibrillar protein and increased insoluble protein and thermal drip. Some deamidation of protein was also observed. We concluded that limited trypsin proteolysis accelerated transglutaminase mediated cross-linking of protein by acyl transfer reactions. Protein polymerization through plastein reactions and intermolecular hydrophobic interaction probably also occurred.

Lysophosphatidic acid (LPA) is a bioactive lysophospholipid mediator that acts through G protein-coupled receptors. Most cell lines in culture express one or more LPA receptors, making it difficult to assign a response to specific LPA receptors. Dissection of the signaling properties of LPA has been hampered by lack of LPA receptor subtype-specific agonists and antagonists. The present study characterizes an ester-linked thiophosphate derivative (1-oleoyl-2-O-methyl-rac-glycerophosphothionate, OMPT) of LPA. OMPT is a functional LPA analogue with potent mitogenic activity in fibroblasts. In contrast to LPA, OMPT does not couple to the pheromone response through the LPA(1) receptor in yeast cells. OMPT induces intracellular calcium increases efficiently in LPA(3) receptor-expressing Sf9 cells but poorly in LPA(2) receptor-expressing cells. Guanosine 5'-O-(3-[(35)S]thio)triphosphate binding assays in mammalian cells showed that LPA exhibits agonistic activity on all three LPA receptor subtypes, whereas OMPT has a potent agonistic effect only on the LPA(3) receptor. In transiently transfected HEK293 cells, OMPT stimulates mitogen-activated protein kinases through the LPA(3) but not the LPA(1) or LPA(2) receptors. Furthermore, OMPT-induced intracellular calcium mobilization in mammalian cells is efficiently inhibited by the LPA(1)/LPA(3) receptor-selective antagonist VPC12249. These results establish that OMPT is an LPA(3)-selective agonist. OMPT binding to the LPA(3) receptor in mammalian cells is sufficient to elicit multiple responses, including activation of G proteins, calcium mobilization, and activation of mitogen-activated protein kinases. Thus OMPT offers a powerful probe for the dissection of LPA signaling events in complex mammalian systems.