15 results on '"Ninfa, Ramírez-Durán"'
Search Results
2. Chronic consumption of sweeteners in mice and its effect on the immune system and the small intestine microbiota
- Author
-
Roxana Valdés-Ramos, Jorge Alberto Escoto, Beatriz E. Martínez-Carrillo, J. F. Aguirre-Garrido, Hugo Ramírez-Saad, and Ninfa Ramírez-Durán
- Subjects
Sucralose ,Sucrose ,Medicina ,RC955-962 ,General Biochemistry, Genetics and Molecular Biology ,intestine, small ,sacarosa ,chemistry.chemical_compound ,estevia ,Immune system ,intestino delgado ,Intestinal mucosa ,stevia ,Arctic medicine. Tropical medicine ,medicine ,Edulcorantes ,gastrointestinal microbiome ,Lamina propria ,biology ,edulcorantes ,Interleukin ,sucrose ,Molecular biology ,Small intestine ,medicine.anatomical_structure ,chemistry ,biology.protein ,Sweetening agents ,Medicine ,Antibody ,microbioma gastrointestinal - Abstract
Resumen Introducción. Los edulcorantes son aditivos que se consumen en los alimentos. Pueden ser naturales (sacarosa y estevia) o artificiales (sucralosa). Actualmente, se consumen rutinariamente en múltiples productos, y sus efectos en la mucosa y la microbiota del intestino delgado aún son controversiales Objetivo. Relacionar el consumo de edulcorantes y su efecto en el sistema inmunitario y la microbiota del intestino delgado en ratones CD1. Materiales y métodos. Se utilizaron 54 ratones CD1 de tres semanas de edad divididos en tres grupos: un grupo de tres semanas sin tratamiento, un grupo tratado durante seis semanas y un grupo tratado durante 12 semanas. Se les administró sacarosa, sucralosa y estevia. A partir del intestino delgado, se obtuvieron linfocitos B CD19+ y células IgA+, TGF-β (Transforming Growth Factor-beta) o el factor de crecimiento transformador beta (TGF-beta), IL-12 e IL-17 de las placas de Peyer y de la lámina propia. De los sólidos intestinales se obtuvo el ADN para identificar las especies bacterianas. Resultados. Después del consumo de sacarosa y sucralosa durante 12 semanas, se redujeron las comunidades bacterianas, la IgA+ y el TGF-beta, se aumentó el CD19+, y además, se incrementaron la IL-12 y la IL-17 en las placas de Peyer; en la lámina propia, aumentaron todos estos valores. En cambio, con la estevia mejoraron la diversidad bacteriana y el porcentaje de linfocitos CD19+, y hubo poco incremento de IgA+, TGF- β e IL-17, pero con disminución de la IL-17. Conclusión. La sacarosa y la sucralosa alteraron negativamente la diversidad bacteriana y los parámetros inmunitarios después de 12 semanas, en contraste con la estevia que resultó benéfica para la mucosa intestinal. Abstract Introduction: Sweeteners are additives used in different foods. They can be natural (sucrose and stevia) or artificial (sucralose). Currently, they are routinely consumed in multiple products and their effects on the mucosa of the small intestine and its microbiota are still controversial. Objective: To relate the consumption of sweeteners and their effect on the immune system and the microbiota of the small intestine in CD1 mice. Materials and methods: We used 54 three-week-old CD1 mice divided into three groups in the experiments: 1) A group of three weeks without treatment, 2) a group treated for six weeks, and 3) a group treated for 12 weeks using sucrose, sucralose, and stevia. We obtained CD19+ B lymphocytes, IgA+ antibodies, transforming growth factor-beta (TGF-b), and interleukins 12 and 17 (IL-12 and -17) from Peyer's patches and lamina propria cells while DNA was obtained from intestinal solids to identify bacterial species. Results: After 12 weeks, sucrose and sucralose consumption caused a reduction in bacterial communities with an increase in CD19+, a decrease in IgA+ and TGF-b, and an increase in IL-12 and -17 in the Peyer's patches while in the lamina propria there was an increase in all parameters. In contrast, stevia led to an improvement in bacterial diversity and percentage of CD19+ lymphocytes with minimal increase in IgA+, TGF-b, and IL-12, and a decrease in IL-17. Conclusion: Sucrose and sucralose caused negative alterations in bacterial diversity and immune parameters after 12 weeks; in contrast, stevia was beneficial for the intestinal mucosa.
- Published
- 2021
3. Genotoxicity of Mercury and Its Derivatives Demonstrated In Vitro and In Vivo in Human Populations Studies. Systematic Review
- Author
-
Lilia Patricia Bustamante-Montes, Ninfa Ramírez-Durán, Mirta Milić, Juana Sánchez-Alarcón, Rafael Valencia-Quintana, and Keila Isaac-Olivé
- Subjects
Chemical Health and Safety ,Health, Toxicology and Mutagenesis ,Chemical technology ,micronucleus assay ,chemistry.chemical_element ,Sister chromatid exchange ,TP1-1185 ,Pharmacology ,Toxicology ,medicine.disease_cause ,Mercury (element) ,Comet assay ,chemistry.chemical_compound ,chemistry ,comet assay ,chromosomal aberrations ,sister chromatid exchange ,In vivo ,Micronucleus test ,medicine ,Systematic Review ,Methylmercury ,Genotoxicity ,Carcinogen - Abstract
Beside partial coverage in three reviews so far (1994, 2009, 2019), there is no review on genotoxic studies dealing with mercury (Hg) and human exposure using the most usual genotoxic assays: sister chromatid exchanges (SCE), chromosomal aberrations (CA), cytochalasin B blocked micronucleus assay (CBMN), and single-cell gel electrophoresis (SCGE or alkaline comet assay). Fifty years from the first Hg genotoxicity study and with the Minamata Convention in force, the genotoxic potential of Hg and its derivatives is still controversial. Considering these antecedents, we present this first systematic literature overview of genotoxic studies dealing with Hg and human exposure that used the standard genotoxic assays. To date, there is not sufficient evidence for Hg human carcinogen classification, so the new data collections can be of great help. A review was made of the studies available (those published before the end of October 2021 on PubMed or Web of Science in English or Spanish language) in the scientific literature dealing with genotoxic assays and human sample exposure ex vivo, in vivo, and in vitro. Results from a total of 66 articles selected are presented. Organic (o)Hg compounds were more toxic than inorganic and/or elemental ones, without ruling out that all represent a risk. The most studied inorganic (i)Hg compounds in populations exposed accidentally, occupationally, or iatrogenically, and/or in human cells, were Hg chloride and Hg nitrate and of the organic compounds, were methylmercury, thimerosal, methylmercury chloride, phenylmercuric acetate, and methylmercury hydroxide.
- Published
- 2021
4. First Report of Multi-resistant Escherichia fergusonii Isolated from Children Under Two Months of Age in Intensive Care Unit
- Author
-
Horacio Sandoval Trujillo, Ninfa Ramírez Durán, Mildred Azucena Rivera Galindo, Luz Marcela Caro Gonzalez, Gauddy Lizeth Manzanares Leal, Hugo Mendieta Zeron, and Erika Santos Ramírez
- Subjects
0301 basic medicine ,Microbiology (medical) ,Bacilli ,medicine.medical_specialty ,Neonatal intensive care unit ,medicine.drug_class ,Avibactam ,030106 microbiology ,Antibiotics ,Ceftazidime ,Microbiology ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,law ,Internal medicine ,medicine ,biology ,business.industry ,Escherichia fergusonii ,biology.organism_classification ,Intensive care unit ,030104 developmental biology ,Infectious Diseases ,chemistry ,Ceftolozane ,business ,medicine.drug - Abstract
Background: Gram-negative bacilli are primarily responsible for the most common pediatric infections. Frequently, Escherichia fergusonii is identified as E. coli because of its close genetic proximity. Objectives: We aimed at the isolation and identification of multi-resistant strains of E. fergusonii, affecting children under two months of age. Methods: Strains were isolated from infectious processes and were identified phenotypically and molecularly. The microdilution method (MicroScan, autoSCAN-4) and the disk diffusion method (modified Kirby Bauer) were used to analyze antibiotic susceptibility. Results: Strains isolated were multi-resistant. Molecular identification provided the correct taxonomic assignment. Escherichia fergusonii strains were wrongly identified as E. coli with the phenotypic identification method. In addition, Pseudomonas aeruginosa and Klebsiella pneumoniae were identified. The best sensitivity results were obtained with Ceftazidime/avibactam and ceftolozane/tazobactam. Conclusions: We provided the first report of isolation and identification of multi-resistant E. fergusonii strains affecting children under two months of age in a neonatal intensive care unit.
- Published
- 2021
5. Generation of electrical energy in a microbial fuel cell coupling acetate oxidation to Fe3+ reduction and isolation of the involved bacteria
- Author
-
Jorge Serment-Guerrero, Ninfa Ramírez-Durán, Gauddy Lizeth Manzanares-Leal, Karina Becerril-Varela, and Claudia Guerrero-Barajas
- Subjects
Microbial fuel cell ,biology ,Physiology ,Chemistry ,Cupriavidus metallidurans ,Microorganism ,Substrate (chemistry) ,General Medicine ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Redox ,Citrobacter freundii ,Azospira oryzae ,Sulfate-reducing bacteria ,Biotechnology ,Nuclear chemistry - Abstract
An iron reducing enrichment was obtained from sulfate reducing sludge and was evaluated on the capability of reducing Fe3+ coupled to acetate oxidation in a microbial fuel cell (MFC). Three molar ratios for acetate/Fe3+ were evaluated (2/16, 3.4/27 and 6.9/55 mM). The percentages of Fe3+ reduction were in a range of 80–90, 60–70 and 40–50% for the MFCs at closed circuit for the molar ratios of 2/16, 3.4/27 and 6.9/55 mM, respectively. Acetate consumption was in a range of 80–90% in all cases. The results obtained at closed circuit for current density were: 11.37 mA/m2, 4.5 mA/m2 and 7.37 mA/m2 for the molar ratios of 2/16, 3.4/27 and 6.9/55 mM, respectively. Some microorganisms that were isolated and identified in the MFCs were Azospira oryzae, Cupriavidus metallidurans CH34, Enterobacter bugandensis 247BMC, Citrobacter freundii ATCC8090 and Citrobacter murliniae CDC2970-59, these bacteria have been reported as exoelectrogens in MFC and in MFC involving metals removal but not all of them have been reported to utilize acetate as preferred substrate. The results demonstrate that the isolates can utilize acetate as the sole source of carbon and suggest that Fe3+ reduction was carried out by a combination of different mechanisms (direct contact and redox mediators) utilized by the bacteria identified in the MFC. Storage of the energy generated from the 2/16 mM MFC system arranged in a series of three demonstrated that it is possible to utilize the energy to charge a battery.
- Published
- 2021
6. Draft Genome Sequence of Saccharomonospora piscinae KCTC 19743 T , an Actinobacterium Containing Secondary Metabolite Biosynthetic Gene Clusters
- Author
-
Horacio Sandoval-Trujillo, Cristina Sánchez-Porro, Scarlett Alonso-Carmona, Rafael R. de la Haba, Ninfa Ramírez-Durán, Antonio Ventosa, Blanca Vera-Gargallo, Universidad de Sevilla. Departamento de Microbiología y Parasitología, Ministerio de Ciencia, Innovación y Universidades (MICINN). España, Junta de Andalucía, and Universidad de Sevilla
- Subjects
Whole genome sequencing ,chemistry.chemical_classification ,0303 health sciences ,030306 microbiology ,Genome Sequences ,Computational biology ,Biology ,Secondary metabolite ,Genome ,03 medical and health sciences ,chemistry.chemical_compound ,Open reading frame ,Immunology and Microbiology (miscellaneous) ,Biosynthesis ,chemistry ,Nonribosomal peptide ,Polyketide synthase ,Genetics ,biology.protein ,medicine ,Molecular Biology ,Gene ,030304 developmental biology ,medicine.drug - Abstract
The draft genome sequence of Saccharomonospora piscinae KCTC 19743T, with a size of 4,897,614 bp, was assembled into 11 scaffolds containing 4,561 open reading frames and a G+C content of 71.0 mol%. Polyketide synthase and nonribosomal peptide synthetase gene clusters, which are responsible for the biosynthesis of several biomolecules, were identified and located in different regions in the genome.
- Published
- 2020
7. DETECCIÓN Y AISLAMIENTO DE MICROORGANISMOS EXOELECTRÓGENOS A PARTIR DE LODOS DEL RÍO LERMA, ESTADO DE MÉXICO, MÉXICO
- Author
-
Jorge Humberto Serment Guerrero, Ninfa Ramírez Durán, Eric Arturo Lara Rivera, Karina Becerril Varela, and Sergio Suárez Contreras
- Subjects
chemistry.chemical_classification ,Exoelectrogen ,Microbial fuel cell ,chemistry ,biology ,Microorganism ,Environmental engineering ,Organic matter ,biology.organism_classification ,Pollution ,Waste Management and Disposal ,Bacteria ,Geobacter - Abstract
Some microorganisms (such as bacteria) are capable of producing renewable energy without harassing the environment. Microbial fuel cells (MFC) offer thepossibility of transforming organic matter into electricity by using the metabolism of these microorganisms, creating ions within the subproducts of the metabolism that go to the extracellular environment. An electrode is set into the MFC that works as a final acceptor that attracts the electrons released by the microorganisms after consuming organic matter. To date, many microorganisms with the capability of liberating electrons through their metabolism have been identified. However only few are able of doing so without chemical mediators, which is why the search of new exoelectrogen microorganisms is of great importance. On the present work, two different MFC were employed for optimizing the isolation of exoelectrogenic bacteria gathered from mud of the Lerma river and from the Centro Interamericano de Recursos del Agua water treatment plant. Voltage generated by these microorganisms was compared with that created by Geobacter sulfurrenducens , one of the most efficient bacteria in this field. Overall, the obtained data show that the isolated bacteria at the National Institute of Nuclear Research facilities (identified by ribosomal RNA as Clostridium sordellii and C. bifermentans ) have a good voltage generation, although not as good as the one from G. sulfurrenducens . Results indicate that the strategy employed in the research is suitable for efficiently locating, isolating and identifying exoelectrogenic bacteria that may be used later on for the in situ generation of electricity in polluted areas.
- Published
- 2017
8. Molecular Identification ofMycobacteriumSpecies of Public Health and Veterinary Importance from Cattle in the South State of México
- Author
-
Ninfa Ramírez Durán, Pablo Moreno Pérez, Keila Isaac-Olivé, Horacio Sandoval Trujillo, Nallely Rivero Perez, Adrian Zaragoza Bastida, and Benjamín Valladares Carranza
- Subjects
0301 basic medicine ,Microbiology (medical) ,Veterinary medicine ,Tuberculosis ,Article Subject ,030106 microbiology ,Infectious and parasitic diseases ,RC109-216 ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Genus ,23S ribosomal RNA ,Molecular marker ,medicine ,030212 general & internal medicine ,Gene ,biology ,16S ribosomal RNA ,biology.organism_classification ,medicine.disease ,QR1-502 ,Infectious Diseases ,chemistry ,Nontuberculous mycobacteria ,Research Article ,Mycobacterium - Abstract
Mycobacteriumgenus causes a variety of zoonotic diseases. The best known example is the zoonotic tuberculosis due toM. bovis. Much less is known about “nontuberculous mycobacteria (NTM),” which are also associated with infections in humans. The Mexican standard NOM-ZOO-031-1995 regulates the presence ofM. bovisin cattle; however, no regulation exists for the NTM species. The objective of this study was to isolate and identify nontuberculous mycobacteria species from cattle of local herds in the south region of the State of Mexico through the identification and detection of the 100 bp molecular marker in the 23S rRNA gene with subsequent sequencing of the 16S rRNA gene. Milk samples (35) and nasal exudate samples (68) were collected. From the 108 strains isolated, 39 were selected for identification. Thirteen strains isolated from nasal exudates amplified the 100 bp molecular marker and were identified asM. neoaurum(six strains),M. parafortuitum(four strains),M. moriokaense(two strains), andM. confluentis(one strain). ExceptM. parafortuitum, the other species identified are of public health and veterinary concern because they are pathogenic to humans, especially those with underlying medical conditions.
- Published
- 2017
9. Geno- and cytotoxicity induced on Cyprinus carpio by aluminum, iron, mercury and mixture thereof
- Author
-
Youssef Paolo Mendoza-Zenil, R.C. Rodríguez Martín-Doimeadios, Nuria Rodríguez-Fariñas, Ricardo Pérez-Pastén Borja, Armando Elizalde-Velázquez, Hariz Islas-Flores, Leobardo Manuel Gómez-Oliván, Marcela Galar-Martínez, Sandra García-Medina, Nely SanJuan-Reyes, and Ninfa Ramírez-Durán
- Subjects
0301 basic medicine ,Carps ,Iron ,Health, Toxicology and Mutagenesis ,chemistry.chemical_element ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Cyprinus ,Industrial wastewater treatment ,03 medical and health sciences ,Common carp ,medicine ,Animals ,Cytotoxicity ,0105 earth and related environmental sciences ,Micronucleus Tests ,biology ,Caspase 3 ,Public Health, Environmental and Occupational Health ,Mercury ,General Medicine ,biology.organism_classification ,Pollution ,Mercury (element) ,Comet assay ,030104 developmental biology ,chemistry ,Environmental chemistry ,Micronucleus test ,Comet Assay ,Biomarkers ,Water Pollutants, Chemical ,Genotoxicity ,Aluminum ,DNA Damage - Abstract
Metals such as Al, Fe and Hg are used in diverse anthropogenic activities. Their presence in water bodies is due mainly to domestic, agricultural and industrial wastewater discharges and constitutes a hazard for the organisms inhabiting these environments. The present study aimed to evaluate geno- and cytotoxicity induced by Al, Fe, Hg and the mixture of these metals on blood of the common carp Cyprinus carpio. Specimens were exposed to the permissible limits in water for human use and consumption according to the pertinent official Mexican norm [official Mexican norm NOM-127-SSA1-1994] Al (0.2mgL-1), Fe (0.3mgL-1), Hg (0.001mgL-1) and their mixture for 12, 24, 48, 72 and 96h. Biomarkers of genotoxicity (comet assay and micronucleus test) and cytotoxicity (caspase-3 activity and TUNEL assay) were evaluated. Significant increases relative to the control group (p
- Published
- 2017
10. In vitro irradiation of doxorubicin with 18F-FDG Cerenkov radiation and its potential application as a theragnostic system
- Author
-
Eugenio Torres-García, Miguel A. Camacho-López, Enrique Morales-Avila, Keila Isaac-Olivé, Myrna Luna-Gutiérrez, Liliana Aranda-Lara, Mariana Sánchez-Holguín, Ninfa Ramírez-Durán, and Hilda Angeline Quintos-Meneses
- Subjects
0303 health sciences ,Radiation ,Radiological and Ultrasound Technology ,Nuclear imaging ,Chemistry ,medicine.medical_treatment ,030303 biophysics ,Biophysics ,Photodynamic therapy ,02 engineering and technology ,021001 nanoscience & nanotechnology ,In vitro ,03 medical and health sciences ,medicine ,Cytotoxic T cell ,Radiology, Nuclear Medicine and imaging ,Doxorubicin ,Irradiation ,0210 nano-technology ,Cytotoxicity ,Laser beams ,medicine.drug - Abstract
Doxorubicin (DOX), an effective chemotherapeutic agent, has a wide excitation band centred at 480 nm. Cerenkov radiation (CR) is considered an internal light source in photodynamic therapy (PDT). DOX could be photoactivated by CR and thus, enhancing its cytotoxicity. In this work, 18F-FDG was used to evaluate the effect of Cerenkov radiation on DOX, in comparison to irradiation with a 450-nm laser beam, in terms of ROS production. The production of 1O2 and O2⁎- reactive species during DOX irradiation was detected indirectly by ABMA and DCPIP bleaching, respectively. The cytotoxic effect of the DOX / 18F-FDG CR system was evaluated in the T47D breast cancer cell line. The irradiation of DOX produced 1O2 and O2⁎- species using both 18F-FDG CR and a 450-nm laser beam. The majority reactive species produced in both cases was 1O2; a favourable result, given the greater cytotoxicity of this species. The viability of T47D cells in presence of DOX (5 nM), 18F-FDG (37.5 μCi) and DOX (5 nM)/18F-FDG (37.5 μCi) was (86 ± 9)%, (84 ± 8)% and (64 ± 5)%, respectively; these results suggest a synergistic cytotoxic effect derived from the cytotoxic activity of DOX and its photoactivation by 18F-FDG CR. It is worth noting that the system could be optimized in terms of DOX concentration and 18F-FDG activity for better results. Due to the fact that 18F-FDG is widely used in nuclear imaging, the DOX/18F-FDG system also possesses theragnostic characteristics. Thus, in this work, it is demonstrated that DOX can be used in a dual therapy system based on chemotherapy-PDT when 18F-FDG CR is used as a DOX excitation source.
- Published
- 2020
11. Chronic Consumption of Sweeteners and Its Effect on Glycaemia, Cytokines, Hormones, and Lymphocytes of GALT in CD1 Mice
- Author
-
Cristian Angel Rosales-Gómez, Roxana Valdés-Ramos, Aldo Arturo Reséndiz-Albor, Talia Mondragón-Velásquez, Beatriz E. Martínez-Carrillo, Ninfa Ramírez-Durán, and Jorge Alberto Escoto-Herrera
- Subjects
0301 basic medicine ,Blood Glucose ,medicine.medical_specialty ,Sucralose ,Article Subject ,Lymphoid Tissue ,media_common.quotation_subject ,medicine.medical_treatment ,T-Lymphocytes ,lcsh:Medicine ,030209 endocrinology & metabolism ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Immune system ,Insulin resistance ,Intestinal mucosa ,Internal medicine ,medicine ,Animals ,Humans ,Insulin ,Lymphocytes ,Intestinal Mucosa ,media_common ,B-Lymphocytes ,General Immunology and Microbiology ,Leptin ,lcsh:R ,Appetite ,General Medicine ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,chemistry ,Sweetening Agents ,Insulin Resistance ,hormones, hormone substitutes, and hormone antagonists ,Hormone ,Research Article - Abstract
Background. The consumption of sweeteners has increased in recent years, being used to control body weight and blood glucose. However, they can cause increased appetite, modification of immune function, and secretion of hormones in the GALT.Objective. To assess the effect of chronic sweetener consumption on glycaemia, cytokines, hormones, and GALT lymphocytes in CD1 mice.Material and Methods. 72 CD1 mice divided into 3 groups were used: (a) baseline, (b) middle, and (c) final. Groups (b) and (c) were divided into 4 subgroups: (i) Control, (ii) Sucrose, (iii) Sucralose, and (iv) Stevia. The following were determined: body weight, hormones (GIP, insulin, and leptin), lymphocytes CD3+T cells and CD19+B cells, IgA+plasma cells, and cytokines (IL-4, IL-5, IFN-γ, and TNF-α).Results. Sucralose reduces secretion of GIP and glycaemia but does not modify insulin concentration, increases body weight, and reduces food intake. Stevia increases the secretion of GIP, insulin, leptin, body weight, and glycaemia but keeps food consumption normal. Sucralose and Stevia showed a higher percentage of CD3+T cells, CD19+B cells, and IgA+plasma cells in Peyer’s patches, but only Stevia in lamina propria.Conclusion. Sweeteners modulate the hormonal response of cytokines and the proliferation of lymphocytes in the intestinal mucosa.
- Published
- 2018
- Full Text
- View/download PDF
12. Bacterial Treatment of Pharmaceutical Industry Effluents
- Author
-
Pablo Antonio Moreno-Pérez, Ángel H. Sandoval-Trujillo, and Ninfa Ramírez-Durán
- Subjects
Active ingredient ,Naproxen ,Chemistry ,medicine.drug_class ,Sulfamethoxazole ,Chloramphenicol ,0208 environmental biotechnology ,Antibiotics ,Clofibric acid ,Erythromycin ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,020801 environmental engineering ,chemistry.chemical_compound ,Personal hygiene ,Environmental chemistry ,medicine ,0105 earth and related environmental sciences ,medicine.drug - Abstract
Pharmaceutical products are substances used mainly for therapeutic purposes. They include a broad group of medications such as antidepressants, antibiotics, bactericides, and personal hygiene products. At world level, 100,000 tons of active pharmaceutical ingredients are manufactured yearly. In general, they have a molecular mass of
- Published
- 2017
13. Draft Genome Sequence of Saccharomonospora sp. Strain LRS4.154, a Moderately Halophilic Actinobacterium with the Biotechnologically Relevant Polyketide Synthase and Nonribosomal Peptide Synthetase Systems
- Author
-
Scarlett Alonso-Carmona, Horacio Sandoval-Trujillo, Antonio Ventosa, Blanca Vera-Gargallo, Rafael R. de la Haba, Ninfa Ramírez-Durán, Universidad de Sevilla. Departamento de Microbiología y Parasitología, and Ministerio de Economia, Industria y Competitividad (MINECO). España
- Subjects
0301 basic medicine ,Whole genome sequencing ,chemistry.chemical_classification ,Strain (chemistry) ,Biology ,Genome ,03 medical and health sciences ,Open reading frame ,030104 developmental biology ,Biochemistry ,chemistry ,Nonribosomal peptide ,Polyketide synthase ,Genetics ,biology.protein ,ORFS ,Molecular Biology ,Gene - Abstract
The draft genome sequence of Saccharomonospora sp. strain LRS4.154, a moderately halophilic actinobacterium, has been determined. The genome has 4,860,108 bp, a G+C content of 71.0%, and 4,525 open reading frames (ORFs). The clusters of PKS and NRPS genes, responsible for the biosynthesis of a large number of biomolecules, were identified in the genome.
- Published
- 2017
14. Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence, Emission Fluorescence, and Synchronous Fluorescence: Comparative Study
- Author
-
Ángel H. Sandoval-Trujillo, Ninfa Ramírez-Durán, Jessica Marlene García-Macedo, Reyna del Carmen Lara-Severino, Keila Isaac-Olivé, Leobardo Manuel Gómez-Oliván, and Miguel A. Camacho-López
- Subjects
Article Subject ,General Chemical Engineering ,Analytical chemistry ,lcsh:Analytical chemistry ,Quantum yield ,010501 environmental sciences ,01 natural sciences ,Analytical Chemistry ,Actinobacteria ,chemistry.chemical_compound ,Bioremediation ,Instrumentation ,0105 earth and related environmental sciences ,Detection limit ,Anthracene ,lcsh:QD71-142 ,biology ,010401 analytical chemistry ,biology.organism_classification ,Fluorescence ,0104 chemical sciences ,Computer Science Applications ,chemistry ,Kocuria rosea ,Excitation ,Research Article - Abstract
Polycyclic aromatic hydrocarbons (PAHs) are compounds that can be quantified by fluorescence due to their high quantum yield. Haloalkalitolerant bacteria tolerate wide concentration ranges of NaCl and pH. They are potentially useful in the PAHs bioremediation of saline environments. However, it is known that salinity of the sample affects fluorescence signal regardless of the method. The objective of this work was to carry out a comparative study based on the sensitivity, linearity, and detection limits of the excitation, emission, and synchronous fluorescence methods, during the quantification of the residual anthracene concentration from the following haloalkalitolerant actinomycetes culturesKocuria rosea, Kocuria palustris, Microbacterium testaceum, and 4 strains ofNocardia farcinica, in order to establish the proper fluorescence method to study the PAHs biodegrading capacity of haloalkalitolerant actinobacteria. The study demonstrated statistical differences among the strains and among the fluorescence methods regarding the anthracene residual concentration. The results showed that excitation and emission fluorescence methods performed very similarly but sensitivity in excitation fluorescence is slightly higher. Synchronous fluorescence usingΔλ=150 nm is not the most convenient method. Therefore we propose the excitation fluorescence as the fluorescence method to be used in the study of the PAHs biodegrading capacity of haloalkalitolerant actinomycetes.
- Published
- 2016
15. Amplification of the specific insertion in the rRNA 23S gene, as a criterion of purity in preparation of actinomycetes DNA
- Author
-
L.M. Melgoza-Contreras, H. Ramírez-Saad, H. Sandoval, and Ninfa Ramírez-Durán
- Subjects
Genetics ,Microorganism ,Ribosomal RNA ,Biology ,biology.organism_classification ,Insert (molecular biology) ,chemistry.chemical_compound ,Infectious Diseases ,chemistry ,Nucleic acid ,Ribosomal DNA ,Gene ,Bacteria ,DNA - Abstract
Actinomycetes are a group of Gram-positive filamentous bacteria. They are widely distributed microorganisms, that are prevalent in tropical and subtropical areas. In addition to the pathogenic species, some actinomycetes species are of great biotechnological importance. Nowadays, the isolation and amplification of nucleic acids is an important technique in most molecular biology research laboratories and the quality and purity of the DNA used for amplification reactions is critical. This paper describes a rapid method to verify the purity of DNA by preliminary amplification of an insert in the rRNA 23S gene.
- Published
- 2006
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.