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Expansins are cell wall (CW) proteins that mediate the CW loosening and regulate salt tolerance in a positive or negative way. However, the role of Populus trichocarpa expansin A6 (PtEXPA6) in salt tolerance and the relevance to cell wall loosening is still unclear in poplars. PtEXPA6 gene was transferred into the hybrid species, Populus alba × P. tremula var. glandulosa (84K) and Populus tremula × P. alba INRA ‘717-1B4’ (717-1B4). Under salt stress, the stem growth, gas exchange, chlorophyll fluorescence, activity and transcription of antioxidant enzymes, Na+ content, and Na+ flux of root xylem and petiole vascular bundle were investigated in wild-type and transgenic poplars. The correlation analysis and principal component analysis (PCA) were used to analyze the correlations among the characteristics and principal components. Our results show that the transcription of PtEXPA6 was downregulated upon a prolonged duration of salt stress (48 h) after a transient increase induced by NaCl (100 mM). The PtEXPA6-transgenic poplars of 84K and 717-1B4 showed a greater reduction (42–65%) in stem height and diameter growth after 15 days of NaCl treatment compared with wild-type (WT) poplars (11–41%). The Na+ accumulation in roots, stems, and leaves was 14–83% higher in the transgenic lines than in the WT. The Na+ buildup in the transgenic poplars affects photosynthesis; the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT); and the transcription of PODa2, SOD [Cu-Zn], and CAT1. Transient flux kinetics showed that the Na+ efflux of root xylem and leaf petiole vascular bundle were 1.9–3.5-fold greater in the PtEXPA6-transgenic poplars than in the WT poplars. PtEXPA6 overexpression increased root contractility and extensibility by 33% and 32%, indicating that PtEXPA6 increased the CW loosening in the transgenic poplars of 84K and 717-1B4. Noteworthily, the PtEXPA6-promoted CW loosening was shown to facilitate Na+ efflux of root xylem and petiole vascular bundle in the transgenic poplars. We conclude that the overexpression of PtEXPA6 leads to CW loosening that facilitates the radial translocation of Na+ into the root xylem and the subsequent Na+ translocation from roots to leaves, resulting in an excessive Na+ accumulation and consequently, reducing salt tolerance in transgenic poplars. Therefore, the downregulation of PtEXPA6 in NaCl-treated Populus trichocarpa favors the maintenance of ionic and reactive oxygen species (ROS) homeostasis under long-term salt stress.

The toxic metal cadmium (Cd) poses a serious threat to plant growth and human health. Populus euphratica calcium-dependent protein kinase 21 (CPK21) has previously been shown to attenuate Cd toxicity by reducing Cd accumulation, enhancing antioxidant defense and improving water balance in transgenic Arabidopsis. Here, we confirmed a protein–protein interaction between PeCPK21 and Arabidopsis nuclear transcription factor YC3 (AtNF-YC3) by yeast two-hybrid and bimolecular fluorescence complementation assays. AtNF-YC3 was induced by Cd and strongly expressed in PeCPK21-overexpressed plants. Overexpression of AtNF-YC3 in Arabidopsis reduced the Cd inhibition of root length, fresh weight and membrane stability under Cd stress conditions (100 µM, 7 d), suggesting that AtNF-YC3 appears to contribute to the improvement of Cd stress tolerance. AtNF-YC3 improved Cd tolerance by limiting Cd uptake and accumulation, activating antioxidant enzymes and reducing hydrogen peroxide (H2O2) production under Cd stress. We conclude that PeCPK21 interacts with AtNF-YC3 to limit Cd accumulation and enhance the reactive oxygen species (ROS) scavenging system and thereby positively regulate plant adaptation to Cd environments. This study highlights the interaction between PeCPK21 and AtNF-YC3 under Cd stress conditions, which can be utilized to improve Cd tolerance in higher plants.

The state of health (SOH) and remaining useful life (RUL) of lithium-ion batteries are critical indicators for assessing battery reliability and safety management. However, these two indicators are difficult to measure directly, posing a challenge to ensure safe and stable battery operation. This paper proposes a method for estimating SOH and predicting RUL of lithium-ion batteries by charging feature extraction and ridge regression. First, three sets of health feature parameters are extracted from the charging voltage curve. The relationship between these health features and maximum battery capacity is quantitatively evaluated using the correlation analysis method. Then, the ridge regression method is employed to establish the battery aging model and estimate SOH. Meanwhile, a multiscale prediction model is developed to predict changes in health features as the number of charge-discharge cycles increases, combining with the battery aging model to perform multistep SOH estimation for predicting RUL. Finally, the accuracy and adaptability of the proposed method are confirmed by two battery datasets obtained from varying operating conditions. Experimental results demonstrate that the prediction curves can approximate the real values closely, the mean absolute error (MAE) and root mean square error (RMSE) calculations of SOH remain below 0.02, and the maximum absolute error (AE) of RUL is no more than two cycles.

The transcription of glycine-rich RNA-binding protein 2 (PeGRP2) transiently increased in the roots and shoots of Populus euphratica (a salt-resistant poplar) upon initial salt exposure and tended to decrease after long-term NaCl stress (100 mM, 12 days). PeGRP2 overexpression in the hybrid Populus tremula × P. alba ‘717-1B4’ (P. × canescens) increased its salt sensitivity, which was reflected in the plant’s growth and photosynthesis. PeGRP2 contains a conserved RNA recognition motif domain at the N-terminus, and RNA affinity purification (RAP) sequencing was developed to enrich the target mRNAs that physically interacted with PeGRP2 in P. × canescens. RAP sequencing combined with RT-qPCR revealed that NaCl decreased the transcripts of PeGRP2-interacting mRNAs encoding photosynthetic proteins, antioxidative enzymes, ATPases, and Na+/H+ antiporters in this transgenic poplar. Specifically, PeGRP2 negatively affected the stability of the target mRNAs encoding the photosynthetic proteins PETC and RBCMT; antioxidant enzymes SOD[Mn], CDSP32, and CYB1-2; ATPases AHA11, ACA8, and ACA9; and the Na+/H+ antiporter NHA1. This resulted in (i) a greater reduction in Fv/Fm, YII, ETR, and Pn; (ii) less pronounced activation of antioxidative enzymes; and (iii) a reduced ability to maintain Na+ homeostasis in the transgenic poplars during long-term salt stress, leading to their lowered ability to tolerate salinity stress.

Abstract The isotopic fractionation factor and element partition coefficient can be calculated only after the geometric optimization of the molecular clusters is completed. Optimization directly affects the accuracy of some parameters, such as the average bond length, molecular volume, harmonic vibrational frequency, and other thermodynamic parameters. Here, we used the improved volume variable cluster model (VVCM) method to optimize the molecular clusters of a typical oxide, quartz. We documented the average bond length and relative volume change. Finally, we extracted the harmonic vibrational frequencies and calculated the equilibrium fractionation factor of the silicon and oxygen isotopes. Given its performance in geometrical optimization and isotope fractionation factor calculation, we further applied the improved VVCM method to calculate isotope equilibrium fractionation factors of Cd and Zn between the hydroxide (Zn–Al layered double hydroxide), carbonate (cadmium-containing calcite) and their aqueous solutions under superficial conditions. We summarized a detailed procedure and used it to re-evaluate published theoretical results for cadmium-containing hydroxyapatite, emphasizing the relative volume change for all clusters and confirming the optimal point charge arrangement (PCA). The results showed that the average bond length and isotope fractionation factor are consistent with those published in previous studies, and the relative volume changes are considerably lower than the results calculated using the periodic boundary method. Specifically, the average Si–O bond length of quartz was 1.63 Å, and the relative volume change of quartz centered on silicon atoms was − 0.39%. The average Zn–O bond length in the Zn–Al-layered double hydroxide was 2.10 Å, with a relative volume change of 1.96%. Cadmium-containing calcite had an average Cd–O bond length of 2.28 Å, with a relative volume change of 0.45%. At 298 K, the equilibrium fractionation factors between quartz, Zn–Al-layered double hydroxide, cadmium-containing calcite, and their corresponding aqueous solutions were $$\Delta ^{30/28} {\text{Si}}_{{{\text{Qtz-H}}_{4} {\text{SiO}}_{4} }} = 2.20{\permil} $$ Δ 30 / 28 Si Qtz-H 4 SiO 4 = 2.20 ‰ , $$\Delta^{18/16} {\text{O}}_{ {\text{Qtz}}{-} ( {\text{H}}_{2} {\text{O}} )_{\text{n}}} = 36.05{\permil}$$ Δ 18 / 16 O Qtz - ( H 2 O ) n = 36.05 ‰ , $$\Delta^{66/64} {\text{Zn}}_{ {\text{Zn}} {-} {\text{Al LDH-Zn}} ( {\text{H}}_{2} {\text{O}} )_{\text{n}}^{2+}} = 1.12{\permil}$$ Δ 66 / 64 Zn Zn - Al LDH-Zn ( H 2 O ) n 2 + = 1.12 ‰ and $$\Delta^{114/110} {\text{Cd}}_{ {\text{(Cd--Cal)-Cd}} ( {\text{H}}_{2} {\text{O}} )_ {\text{n}}^{2 +} } = - 0.26{\permil}$$ Δ 114 / 110 Cd (Cd--Cal)-Cd ( H 2 O ) n 2 + = - 0.26 ‰ respectively. These results strongly support the reliability of the improved VVCM method for geometric optimization of molecular clusters.

Gas-fired boilers, gas stoves, and wall-mounted gas boilers are the main consumers of gas fuel, but they generally encounter problems when operating at high altitudes, such as reduced thermal efficiency and increased pollutant emissions. Previous studies on gas combustion characteristics under sub-atmospheric pressure were mostly carried out in a large space, which is quite different from chamber combustion equipment. Therefore, it is insufficient to guide the design and operation optimization of plateau gas equipment. In this paper, experimentations were carried out to explore the characteristics of a methane diffusion flame under sub-atmospheric pressures. The mass flow rates of methane and air remain consistent under different pressure conditions. The centerline temperature (Tc) distribution, flame appearance, smoke point, CO emission, and NOx emission under different pressures (ranging from 61.66 to 97.75 kPa) were examined under both fuel rich and lean conditions. The results show that Tc at the rear and front of furnace variation with pressure is opposite under fuel-lean and -rich combustion. The Tc at the front of furnace decreases with decreasing pressure, whereas Tc at the rear of furnace increases with decreasing pressure. With decreasing pressure, flame length decreases under lean combustion, but increases under rich combustion. The smoke point fuel flow rate, flame length, and residence time increases with decreasing pressure, following the law of negative exponent. The CO emission decreases with decreasing pressure, which indicates that the reduced pressure makes methane combustion more complete. For NO emission, the reduced pressure results in an opposite tendency under fuel-lean and -rich combustion. With decreasing pressure, the NO emission decreases under fuel-lean combustion but increases under fuel-rich combustion.

High NaCl (200 mM) increases the transcription of phospholipase Dδ (PLDδ) in roots and leaves of the salt-resistant woody species Populus euphratica. We isolated a 1138 bp promoter fragment upstream of the translation initiation codon of PePLDδ. A promoter–reporter construct, PePLDδ-pro::GUS, was introduced into Arabidopsis plants (Arabidopsis thaliana) to demonstrate the NaCl-induced PePLDδ promoter activity in root and leaf tissues. Mass spectrometry analysis of DNA pull-down-enriched proteins in P. euphratica revealed that PeGLABRA3, a basic helix–loop–helix transcription factor, was the target transcription factor for binding the promoter region of PePLDδ. The PeGLABRA3 binding to PePLDδ-pro was further verified by virus-induced gene silencing, luciferase reporter assay (LRA), yeast one-hybrid assay, and electrophoretic mobility shift assay (EMSA). In addition, the PeGLABRA3 gene was cloned and overexpressed in Arabidopsis to determine the function of PeGLABRA3 in salt tolerance. PeGLABRA3-overexpressed Arabidopsis lines (OE1 and OE2) had a greater capacity to scavenge reactive oxygen species (ROS) and to extrude Na+ under salinity stress. Furthermore, the EMSA and LRA results confirmed that PeGLABRA3 interacted with the promoter of AtPLDδ in transgenic plants. The upregulated AtPLDδ in PeGLABRA3-transgenic lines resulted in an increase in phosphatidic acid species under no-salt and saline conditions. We conclude that PeGLABRA3 activated AtPLDδ transcription under salt stress by binding to the AtPLDδ promoter region, conferring Na+ and ROS homeostasis control via signaling pathways mediated by PLDδ and phosphatidic acid.

Bile components play a critical role in maintaining gut microbiota homeostasis. In cholestasis, bile secretion is impaired, leading to liver injury. However, it remains to be elucidated whether gut microbiota plays a role in cholestatic liver injury. Here, we performed a sham operation and bile duct ligation (BDL) in antibiotic-induced microbiome depleted (AIMD) mice and assessed liver injury and fecal microbiota composition in these mice. Significant reductions in gut microbiota richness and diversity were found in AIMD-sham mice when compared to sham controls. Three-day BDL leads to great elevation of plasma ALT, ALP, total bile acids, and bilirubin where reduced diversity of the gut microbiota was also found. AIMD further aggravated cholestatic liver injury evidenced by significantly higher levels of plasma ALT and ALP, associated with further reduced diversity and increased Gram-negative bacteria in gut microbiota. Further analyses revealed increased levels of LPS in the plasma of AIMD-BDL mice where elevated expression of inflammatory genes and decreased expression of hepatic detoxification enzymes were also found in liver when compared to the BDL group. These findings indicate that gut microbiota plays a critical role in cholestatic liver injury. Maintaining its homeostasis may alleviate liver injury in patients with cholestasis.

As one of the key techniques used in the perception layer of the Industrial Internet of Things (IIoT), radio frequency identification (RFID) has been widely applied for object tracing, smart warehouse management, product line monitoring, etc. In most applications, conveyor belts are prevalently implemented to accelerate the sorting efficiency for goods management. However, in such a system, tags quickly go through the reader’s reading range resulting in constant changing of the tag set and limited participating time of moving tags. As a result, it poses more challenges to the tag identification problem in mobile systems than in traditional static applications. In this work, a novel admission-control-based dynamic query tree (ACDQT) protocol is proposed for fast-moving tag identification. In ACDQT, two main strategies are developed, i.e., multi-round admission control (MRAC) and dynamic query tree recognition (DQTR). In MRAC, the reading process of multiple rounds is analyzed, and the number of admitted tags in each round is optimized. Thus, the tag lost ratio is guaranteed, and the identification process can be effectively accelerated. In DQTR, colliding tags are grouped into multiple subsets with the help of consecutive colliding bits in tag responses. By constructing a dynamic query tree, the number of collision slots is greatly reduced, and the identification efficiency in a single round is improved significantly. With MRAC and DQTR, ACDQT can support higher tag flow rate in mobile systems than existing works. Both theoretical analyses and simulation results are presented to demonstrate the effectiveness of ACDQT.

To improve the passive safety of the collision process, we designed a kind of energy absorption device based on cutting shear rings (CSR). It can be installed in a limited space, overcoming the characteristic of traditional energy absorption devices that cannot play their advantages in a narrow space, and it will not be accompanied by the splashing of chips in the process of energy absorption. The impact energy absorption characteristics of the shear rings outside the energy absorption rods are analyzed. The feasibility of the finite element method to study the device’s characteristics is demonstrated through the combination of the drop hammer test and numerical simulation. At the same time, the device’s responses under nine different working cases are simulated, and the impact energy absorption characteristics of the shear rings are analyzed. Moreover, the effects of shear ring thickness and spacing on the device are also analyzed. The results show that there are several peak forces during the energy absorption process, and each peak force is accompanied by the failure of the shear ring. The shear rings’ thickness correlates positively with energy absorption and peak force. But the spacing of shear rings only affects the number of peak forces. There is a linear trend between the shear rings’ spacing and energy absorption, and the slope of the trend increases with the increase in thickness.

Malignant tumors are always a critical threat to human health, with complex pathogenesis, numerous causative factors, and poor prognosis. The features of cancers, such as gene mutations, epigenetic alterations, and the activation and inhibition of signaling pathways in the organism, play important roles in tumorigenesis and prognosis. MicroRNA (miRNA) enables the control of various molecular mechanisms and plays a variety of roles in human cancers, such as radiation sensitivity and tumor immunity, through the regulation of target genes. MiR-149-5p participates in the process and is closely related to lipogenesis, the migration of vascular endothelial cells, and the expression of stem-cell-related proteins. In recent years, its role in cancer has dramatically increased. In this review, we summarize the regular physiological roles of miRNAs, specifically miR-149-5p, in the organism and discuss the tumor-suppressive or oncogenic roles of miR-149-5p in different human cancers with respect to signaling pathways involved in regulation. Possible clinical applications of miR-149-5p in future targeted therapies and prognosis improvement in oncology are suggested.

Over the years, lung cancer remains the leading cause of cancer deaths in worldwide. In view of this, increasingly importance has been attached to the further optimization and improvement of its treatment. Reactive oxygen species (ROS) play a key role in regulating tumor development and anti-cancer treatment. Recently, the development of nanomaterials provides new platforms for ROS-based cancer treatment methods, which can help to reduce side effects and enhance anti-cancer effects. In recent years, a variety of lung cancer treatment models have been reported, such as chemodynamic therapy (CDT), photodynamic therapy (PDT), radiation therapy (RT) and controlled drug release (CDR). In this review, we are going to discuss the possible mechanism of action and current research status of ROS-based nanomaterials in the treatment of lung cancer in order to provide constructive ideas for relative research and expect this work could inspire the future development of novel lung cancer treatments.

Phospholipase Dα (PLDα), which produces signaling molecules phosphatidic acid (PA), has been shown to play a critical role in plants adapting to salt environments. However, it is unclear whether phospholipase Dδ (PLDδ) can mediate the salt response in higher plants. PePLDδ was isolated from salt-resistant Populus euphratica and transferred to Arabidopsis thaliana to testify the salt tolerance of transgenic plants. The NaCl treatment (130 mM) reduced the root growth and whole-plant fresh weight of wild-type (WT) A. thaliana, vector controls (VC) and PePLDδ-overexpressed lines, although a less pronounced effect was observed in transgenic plants. Under salt treatment, PePLDδ-transgenic Arabidopsis exhibited lower electrolyte leakage, malondialdehyde content and H2O2 levels than WT and VC, resulting from the activated antioxidant enzymes and upregulated transcripts of genes encoding superoxide dismutase, ascorbic acid peroxidase and peroxidase. In addition, PePLDδ-overexpressed plants increased the transcription of genes encoding the plasma membrane Na+/H+ antiporter (AtSOS1) and H+-ATPase (AtAHA2), which enabled transgenic plants to proceed with Na+ extrusion and reduce K+ loss under salinity. The capacity to regulate reactive oxygen species (ROS) and K+/Na+ homeostasis was associated with the abundance of specific PA species in plants overexpressing PePLDδ. PePLDδ-transgenic plants retained a typically higher abundance of PA species, 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2), 36:5 (18:2–18:3) and 36:6 (18:3–18:3), under control and saline conditions. It is noteworthy that PA species 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2) and 36:5 (18:2–18:3) markedly increased in response to NaCl in transgenic plants. In conclusion, we suppose that PePLDδ-derived PA enhanced the salinity tolerance by regulating ROS and K+/Na+ homeostasis in Arabidopsis.

With the development of nanotechnology and biotechnology, the nanomachine can be applied to the interior of the human body. In order to achieve the goal of completing complex tasks, measures to connect multiple nanomachines that can complete more simple tasks are taken. This can expand the ability of a single nanomachine to cooperate and share information to complete more complex tasks—namely, the emergence of the Body Area Network (BAN). In response to the requirements of building a BAN, we must first need to solve the communication problem between two nanomachines. Communication networks based on molecular communication (MC), known as “natural body area networks”, are widely used in biomedical fields. With the considerable development of MC theory, it is urgent to set up an experimental platform to verify and guide theoretical modeling. In this paper, a nanomaterial-based MC platform is designed and built to simulate the cardiovascular system. The platform uses the diffusion of nanoscale pigment particles in water solution in silicone tube to achieve communication process and modulates binary sequence information to messenger molecules by on-off keying (OOK). The platform successfully transmits and receives a 17-bit binary sequence to prove its communication possibilities. To assess the platform capabilities, this paper tests the effects of different solution concentrations, pipeline flow rates, and pressure on platform communications. These factors can be used to expand the modulation schemes that the platform can implement. In future work, some nanomaterials that can be used for molecular communication can be applied to the platform to characterize their channel characteristics.

Cadmium (Cd2+) pollution occurring in salt-affected soils has become an increasing environmental concern in the world. Fast-growing poplars have been widely utilized for phytoremediation of soil contaminating heavy metals (HMs). However, the woody Cd2+-hyperaccumulator, Populus × canescens, is relatively salt-sensitive and therefore cannot be directly used to remediate HMs from salt-affected soils. The aim of the present study was to testify whether colonization of P. × canescens with ectomycorrhizal (EM) fungi, a strategy known to enhance salt tolerance, provides an opportunity for affordable remediation of Cd2+-polluted saline soils. Ectomycorrhization with Paxillus involutus strains facilitated Cd2+ enrichment in P. × canescens upon CdCl2 exposures (50 μM, 30 min to 24 h). The fungus-stimulated Cd2+ in roots was significantly restricted by inhibitors of plasmalemma H+-ATPases and Ca2+-permeable channels (CaPCs), but stimulated by an activator of plasmalemma H+-ATPases. NaCl (100 mM) lowered the transient and steady-state Cd2+ influx in roots and fungal mycelia. Noteworthy, P. involutus colonization partly reverted the salt suppression of Cd2+ uptake in poplar roots. EM fungus colonization upregulated transcription of plasmalemma H+-ATPases (PcHA4, 8, 11) and annexins (PcANN1, 2, 4), which might mediate Cd2+ conductance through CaPCs. EM roots retained relatively highly expressed PcHAs and PcANNs, thus facilitating Cd2+ enrichment under co-occurring stress of cadmium and salinity. We conclude that ectomycorrhization of woody hyperaccumulator species such as poplar could improve phytoremediation of Cd2+ in salt-affected areas.

Stomatal regulation is crucial to reduce water consumption under drought conditions. Extracellular ATP (eATP) serves as a signaling agent in stomatal regulation; however, it is less known whether the eATP mediation of stomatal aperture is linked to apyrases (APYs), the principal enzymes that control the concentration of eATP. To clarify the role of APYs in stomatal control, PeAPY1 and PeAPY2 were isolated from Populus euphratica and transferred into Arabidopsis. Compared with the wild-type Arabidopsis and loss-of-function mutants (Atapy1 and Atapy2), PeAPY1- and PeAPY2-transgenic plants decreased stomatal aperture under mannitol treatment (200 mM, 2 h) and reduced water loss during air exposure (90 min). The role of apyrase in stomatal regulation resulted from its control in eATP-regulated stomatal movements and increased stomatal sensitivity to ABA. The bi-phasic dose-responses to applied nucleotides, i.e., the low ATP (0.3–1.0 mM)-promoted opening and high ATP (>2.0 mM)-promoted closure, were both restricted by P. euphratica apyrases. It is noteworthy that eATP at a low concentration (0.3 mM) counteracted ABA action in the regulation of stomatal aperture, while overexpression of PeAPY1 or PeAPY2 effectively diminished eATP promotion in opening, and consequently enhanced ABA action in closure. We postulate a speculative model of apyrase signaling in eATP- and ABA-regulated stomatal movements under drought.

As research on quantum computers and quantum information transmission deepens, the multi-particle and multi-mode quantum information transmission has been attracting increasing attention. For scenarios where multi-parties transmit sequentially increasing qubits, we put forward a novel (N + 1)-party cyclic remote state preparation (RSP) protocol among an arbitrary number of players and a controller. Specifically, we employ a four-party scheme in the case of a cyclic asymmetric remote state preparation scheme and demonstrate the feasibility of the scheme on the IBM Quantum Experience platform. Furthermore, we present a general quantum channel expression under different circulation directions based on the n-party. In addition, considering the impact of the actual environment in the scheme, we discuss the feasibility of the scheme affected by different noises.

The author wishes to make the following corrections to this paper [...]

Non-contact health care monitoring is a unique feature in the emerging 5G networks that is achieved by exploiting artificial intelligence (AI). The ratio of the number of health care problems and patients is increasing exponentially and creating burgeoning data. The integration of AI and Internet of things (IoT) systems enables us to increase the huge volume of data to be generated. The approach by which AI is applied to the IoT systems enhances the intelligence of the health care system. In post-surgery monitoring of the patient, timely consultation is essential before further loss. Unfortunately, even after the advice of the doctor to the patient, he/she may forget to perform the activity in the correct way, which may lead to complications in recovery. In this research, the idea is to design a non-contact sensing testbed using AI for the classification of post-surgery activities. Universal software-defined radio peripheral (USRP) is utilized to collect the data of spinal cord operated patients during weight lifting activity. The wireless channel state information (WCSI) is extracted by using orthogonal frequency division multiplexing (OFDM) technique. AI applies machine learning to classify the correct and wrong way of weight lifting activity that was considered for experimental analysis. The accuracy achieved by the proposed testbed by using a fine K-nearest neighbor (FKNN) algorithm is 99.6%.

Magnesium (Mg) alloys are promising scaffolds for the next generation of cardiovascular stents because of their better biocompatibility and biodegradation compared to traditional metals. However, insufficient mechanical strength and high degradation rate are still the two main limitations for Mg materials. Hydrofluoric acid (HF) treatment and collagen coating were used in this research to improve the endothelialization of two rare earth-based Mg alloys. Results demonstrated that a nanoporous film structure of fluoride with thickness of ~20 µm was formed on the Mg material surface, which improved the corrosion resistance. Primary human coronary artery endothelial cells (HCAECs) had much better attachment, spreading, growth and proliferation (the process of endothelialization) on HF-treated Mg materials compared to bare- or collagen-coated ones.

Sodium chloride (NaCl) induced expression of a jacalin-related mannose-binding lectin (JRL) gene in leaves, roots, and callus cultures of Populus euphratica (salt-resistant poplar). To explore the mechanism of the PeJRL in salinity tolerance, the full length of PeJRL was cloned from P. euphratica and was transformed into Arabidopsis. PeJRL was localized to the cytoplasm in mesophyll cells. Overexpression of PeJRL in Arabidopsis significantly improved the salt tolerance of transgenic plants, in terms of seed germination, root growth, and electrolyte leakage during seedling establishment. Under NaCl stress, transgenic plants retained K+ and limited the accumulation of Na+. PeJRL-transgenic lines increased Na+ extrusion, which was associated with the upward regulation of SOS1, AHA1, and AHA2 genes encoding plasma membrane Na+/proton (H+) antiporter and H+-pumps. The activated H+-ATPases in PeJRL-overexpressed plants restricted the channel-mediated loss of K+ that was activated by NaCl-induced depolarization. Under salt stress, PeJRL⁻transgenic Arabidopsis maintained reactive oxygen species (ROS) homeostasis by activating the antioxidant enzymes and reducing the production of O2− through downregulation of NADPH oxidases. Of note, the PeJRL-transgenic Arabidopsis repressed abscisic acid (ABA) biosynthesis, thus reducing the ABA-elicited ROS production and the oxidative damage during the period of salt stress. A schematic model was proposed to show the mediation of PeJRL on ABA response, and ionic and ROS homeostasis under NaCl stress.