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Improving diagnostic methods for the appointment of effective treatment of animals with urocystitis is an extremely important problem in veterinary medicine. Microscopy of urine sediment is a necessary and important part of general clinical research. With this research method, it is possible to clarify the localization of the inflammatory process in the urinary tract, establish the degree of mucosal damage, differentiate urinary tract diseases from kidney diseases, and conduct differential diagnosis of neoplasms. Urine sediment in dogs suffering from urocystitis revealed postrenal microhematuria (in the field of view of the microscope 5-20 red blood cells), pyuria due to neutrophils (in the field of view 20-100), bacteriuria and the appearance of a significant number of transitional and superficial epithelial cells (in the field of view 3-5 cells), which were placed, both singly and in the form of layers. The admission of a large amount of transitional epithelial cells epithelium into the urine of the male dogs indicated the development of an inflammatory process of bacterial origin in the bladder and distal urethra, sometimes with prostate damage. In 50% of the dogs with urocystitis, crystalluria and spermatorea were noted. Of the unorganized sediments in the urine of experimental animals, crystals of ammonium biurate (acidic uric acid ammonium) were most often found, less often crystals of phosphoric acid calx, and even less often struvite crystals.

The dog`s breed tendency who are get sick the most with urocystitis is analyzed. It have been identified that dogs with diagnosed cystitis have subfebrile fever, moderate tachycardia and tachypnea anxiety when urging for urination, strangury, polakuria, involuntary excretion of muddy urine, and some of them were diagnosed with an increase in volume and pain of the prostate gland. There are significant changes in the morphological blood composition were found in dog's blood, namely low erythrocyte count and low hemoglobin content, and significantly higher leukocyte count, neutrophilia with bands growth and the appearance of young neutrophils compared to healthy animals. In the serum of dogs suffering from urocystitis was revealed a moderate increase in protein due to the increase globulin fraction, increased activity of ALT, AsAT, ALP and decrease of the total calcium and inorganic phosphorus content. A sharp increase in hydrogen index, turbidity, sometimes impurity mucus and a sharp odor of ammonia, protein urobilinogen, bilirubin, nitrites, the appearance of a large amount of sediment in the form of a clot of pus and mucus have been revealed in dogs urine with urinary cystitis.

Calculations by molecular mechanics methods showed that diglycerides containing stearic, oleic, linoleic, or linolenic acids can bind to DNA. The binding energy of diglycerides to the DNA minor groove is higher than that to the major groove. The bond energies of diglycerides to DNA were calculated as the sums of the contributions of the binding energies between DNA and two fatty acid residues. These energies depend on the structure of the fatty acid and the nucleotide composition of DNA and vary from 20 to 120 kcal mol-1. © 2008 Springer Science+Business Media, Inc.