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Meat is the most valuable livestock product since it is one of the main sources of protein for human consumption. Meat quality can be evaluated according to the following parameters: pH, amount of lactic acid, volatile fatty acids, bounded water, solubility of proteins, color, and tenderness. The meat composition and physical properties of muscles have been characterized for ensuring improved eating quality. Thus, the purpose of this paper was to review the major chemical compositional and physicochemical properties of meat and, at the same time, its quality attributes and factors that affect quality of meat. A number of structural features of meat as connective tissue, muscle fibers, and tendon that attaches the muscle to the bone are visible in joint meat examined through naked eyes. Water is quantitatively the most important component of meat comprising up to 75% of weight. Meat is also composed of amino acids, fatty acids, vitamins, minerals, and other important ingredients. Quality factors perceived by consumers are related to sensory attributes (e.g., color, tenderness, and flavor), nutritional properties (e.g., calories, vitamins’ content, and fatty acids’ profile), and appearance (e.g., exudation, marbling, and visible amount of fat). However, fresh meat quality can be defined instrumentally including composition, nutrients, color, water-holding capacity, tenderness, functionality, flavors, spoilage, and contamination. Visual inspection based on sensory quality attributes and different chemical methods are used to analyze meat quality. Other methods such as computer vision and imaging spectroscopy, gas chromatographic analysis, near-infrared technology, dual-energy X-ray absorptiometry, and computerized tomography scanning are also used in the meat industry. So, the aim of the present review is to review quality characteristics of cattle meat and its composition constituents.

Bone processing is an urgent task of rational use of meat industry waste. Disposal of cattle bones can be carried out by different methods. Various methods are used for processing bone raw materials, such as mechanical processing, chemical hydrolysis, ultrasonic processing, etc. Processed meat and bone raw materials in meat and bone paste are chemically exposed to ascorbic acid (0.05 M, 1 h, 25 ° C) and pepsin (pH 2.0, t=10...40 ° C, τ=1...8 h). This allows you to reduce the mass of bone particles in meat and bone paste by 70%. Meat and bone paste subjected to enzyme processing can be used in the technology of meat products.

Meat byproduct is rich source protein for hydrolysis. Pork liver is low value byproduct obtained from meat industry. Therefore, utilization of low value slaughterhouse byproduct enhances the income of meat industry, reduces the cost of disposal and environmental pollution. Physico-chemicals, colour profiles, lipid oxidation, the antioxidant and antimicrobial potential of liver hydrolysate (at three levels T1 = 0.03, T2 = 0.06 and T3 = 0.09% w/w meat emulsion) were comparatively investigated with control (without hydrolysate C = 0) and positive control (PC (BHT = 0.02% w/w meat emulsion)) in meat emulsion. Samples were stored at 4 ± 1 °C under aerobic packaging condition and were drawn at 2 days intervals for analysis. Results revealed that pH values increased significantly (P

The results of microbiological studies of air samples of refrigerating chambers of meat processing enterprises are presented. The quantitative composition of the air microbiota of the chambers of the refrigerating shop was studied. It has been established that the technological regimes for cooling meat in cooled chambers (t = +4 °C) and deep freezing chambers (t = -18 °C and -22 °C) have no bacteriostatic effect on the life activity of mold fungi. The developed disinfecting preparation (hydrogen peroxide (8.0 - 10%), acetic acid (10%), peracetic acid (5.0 - 7.0%), stabilizing additives, water) ensures the destruction of sanitary-indicative microorganisms in cold rooms meat processing plants when applied at a concentration of 0.05% - 60 minutes, 0.1% - 30 minutes, 0.15% - 10 minutes and does not have a toxic effect on meat raw materials that are stored in chambers of the refrigeration shop after disinfection.

Ozone is a GRAS certified cost-effective and eco-friendly technology whose application in food industry is gaining momentum in recent years. It is a powerful oxidizing agent and has a broad spectru...

Nitrate and nitrite salts are important food additives that improve quality, safety and shelf life of meat products. Although their use is regulated, the concern about the use of E-number additives have been pushing researchers and professionals of the meat industry for alternative strategies to ‘clean’ the label of meat products produced with nitrate and/or nitrite salts. Natural extracts from cruciferous vegetables is an interesting strategy to overcome this challenge due to their high nitrate content. This review aims to discuss the main sources of nitrates among cruciferous vegetables and also their technological as natural additives in the production of cured meat products.

Health concerns have motivated salt reduction on diverse foods. Salt in processed meat usually reach high levels, and its reduction is not an easy task, due to its preservative action and expected sensory characteristics in them. Meat industry is looking for alternative solutions for both salt reduction and replacement. The curing time needed to reach the required salt content in meat products can be reduced by the application of ultrasound. Ultrasound technology has been applied for brining, marinating, tumbling, and cooking, with promising results as a useful strategy to reduce the salt content in meat products, such as dry-cured ham, bacon and chicken breast. However, ultrasound parameters should be optimized for each meat product, because structure and composition are diverse among products and the US effects be different. The purpose of this review is to highlight and summarize recent studies on ultrasound application to obtain low salt meat products.

The properties associated with NaCl make it a widely used preservative in the meat industry. However, the growing concern of consumers for their health, and the recommendations of international organizations, force the reformulation of meat products, reducing the salt contents and therefore, giving rise to healthier products. This leads to the search for alternatives that allow to reduce salt content in the product without modifying its technological and sensory properties. In this regard, the replacement by metallic salts is the most common strategy. Recently, its combination with the application of emerging technologies is being evaluated to obtain low-sodium meat products of high quality.

15 pags, 5 figs, 3 tabs. -- Supplementary data to this article can be found online at https://doi.org/10.1016/j.csbj.2021.11.027., Many microorganisms feed on the tissue and recalcitrant bone materials from dead animals, however little is known about the collaborative effort and characteristics of their enzymes. In this study, microbial metagenomes from symbionts of the marine bone-dwelling worm Osedax mucofloris, and from microbial biofilms growing on experimentally deployed bone surfaces were screened for specialized bone-degrading enzymes. A total of 2,043 taxonomically (closest match within 40 phyla) and functionally (1 proteolytic and 9 glycohydrolytic activities) diverse and non-redundant sequences (median pairwise identity of 23.6%) encoding such enzymes were retrieved. The taxonomic assignation and the median identity of 72.2% to homologous proteins reflect microbial and functional novelty associated to a specialized bone-degrading marine community. Binning suggests that only one generalist hosting all ten targeted activities, working in synergy with multiple specialists hosting a few or individual activities. Collagenases were the most abundant enzyme class, representing 48% of the total hits. A total of 47 diverse enzymes, representing 8 hydrolytic activities, were produced in Escherichia coli, whereof 13 were soluble and active. The biochemical analyses revealed a wide range of optimal pH (4.0-7.0), optimal temperature (5-65 °C), and of accepted substrates, specific to each microbial enzyme. This versatility may contribute to a high environmental plasticity of bone-degrading marine consortia that can be confronted to diverse habitats and bone materials. Through bone-meal degradation tests, we further demonstrated that some of these enzymes, particularly those from Flavobacteriaceae and Marinifilaceae, may be an asset for development of new value chains in the biorefinery industry., We acknowledge financial support of ERA-NET Marine Biotechnology (GA no.: 604814) funded under the FP7 ERA-NET scheme and nationally managed from the German Federal Ministry of Education and Research and Norwegian Research Council, the grants PCIN-2017-078 (within the Marine Biotechnology ERA-NET), BIO2017-85522-R, PID2020-112758RB-I00 and PDC2021-121534-I00 from the Ministerio de Economía, Industria y Competitividad, Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación (AEI) (Digital Object Identifier 10.13039/501100011033), Fondo Europeo de Desarrollo Regional (FEDER) and the European Union (“NextGenerationEU/PRTR”), and the grant 2020AEP061 from the Agencia Estatal CSIC. F.J.P and J.S-A. acknowledges grants PID2019-105838RB-C31 and PID2019-105838RB-C33 from the Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación (AEI), Fondo Europeo de Desarrollo Regional (FEDER) and the European Union (EU). Additional funding was received from the Norwegian Biodiversity Information Centre (PA 809116 knr. 47-14) and NORCE Norwegian Research Centre. J.W. acknowledge the use of de.NBI cloud and the support by the High Performance and Cloud Computing Group at the Zentrum für Datenverarbeitung of the Eberhard Karls University of Tübingen and the Federal Ministry of Education and Research (BMBF) through grant no 031 A535A. K.S.M. is supported by intramural funds of the US Department of Health and Human Services (to the National Library of Medicine).

Thermal treatment is a widely applied food processing technology in the meat industry due to its convenience. However, the interpretation of the changes in the bacterial community and quality properties in the thermal processed meat products have not been well established. Therefore, the effects of thermal treatment on the quality characteristics and bacterial communities in meatballs during storage at 4°C were investigated, which will provide a more comprehensive understanding of the influence of thermal treatment on the meat quality. Thermal treatment (121°C, 15 min) decreased the initial total viable bacterial counts by 2.1 log CFU/g and the diversity of the initial bacterial communities in meatballs. Compared with the thermal treatment group, a significantly more rapidly increasing trend of total volatile basic nitrogen and a decreasing trend of pH were observed in the control group. At the end of storage, the bacterial community was dominated by Streptococcus, Acinetobacter and Pseudomonas in the thermal treatment meatballs, whereas Pseudomonas, Pantoea, and Serratia. dominated the bacterial community of the control group. Besides, the predicted metabolic pathways revealed high levels of carbohydrate, amino acid, and lipid metabolism in the control group. This finding could contribute to a deep understanding of the influence of thermal treatment on the meat quality. Moreover, these results could provide a theoretical foundation for the development of alternative and novel nonthermal processing technologies for use in the meat industry., The findings of this study demonstrated that thermal treatment (121°C, 15 min) could significantly decrease bacterial community diversity and the growth of potential spoilage bacteria in meatballs. In particular, thermal treatment could largely decrease the relative abundance of bacterial metabolic pathways in meatballs, such as carbohydrate, amino acid, and lipid metabolism to maintain the freshness of meatballs during storage.

Texture and color are some of the most important qualitative features of meat and its products. They affect the acceptance among consumers, and they are very important parameters of the culinary and meat industry's usefulness of meat. However, cooked meat is usually not enough soft and juicy to satisfy consumers. Researches try to find methods to improve the texture of the meat products. One method is acidic marinating meat. But there are no reports on the use of fermented dairy products (FDP) for meat marinating. The acids contained in FDP can be improved tenderness, juiciness and color of meat products. Also, the use of lower heat treatment temperature for cooking meat positively affects its sensory properties and texture. The aim of the study was the impact of meat marinating in FDP (kefir, yoghurt and buttermilk) marinating time (3, 6, 9 and 12 days) and sous-vide (SV) cooking temperature (vacuum, 60 or 80 °C, 6 h), on texture and color parameters of pork steaks. Marinating the meat increased the tenderness (lowering the hardness and chewiness) of steaks. The best results were obtained by marinating steaks in buttermilk or yoghurt for 6 or 9 days and SV cooking at 60 °C for 6 h. There was no influence of the marinade type and SV cooking temperature on L* and b* color parameters. Redness increased with marinating time and cooking temperature. Kefir and buttermilk did not affect the a* value of SV cooked steaks; however, marinating in yoghurt reduced that value.

BACKGROUND The meat industry is determined to find biodegradable packaging with properties similar to plastic. Furcellaran (FUR) and whey protein isolate (WPI) were used as a film matrix in which Borago officinalis extract (BOE) was incorporated as an antioxidant compound. The film's mechanical properties, water behavior, surface color, and antioxidant power were analyzed.Smoked hams were manufactured using two different types of film application: cured meat covered with film, smoked and cooked or hamsafter smoking, cooking and cooling. Smoked, vacuum packed ham was used as a control sample. The products were stored at 4 °C for 21 days and analyzed every 7 days. RESULTS The elongation at break (EAB) and tensile strength (TS) of FUR/WPI films without the extract were 6.30% and 20.59 MPa, respectively, and after incorporating BOE, the EAB and TS were 24.30% and 15.33 MPa, respectively. The films with BOE were darker and had greater antioxidant capacity. The water content and activity in the products with films decreased along with storage time while the control remained stable. The results of microbiological, oxidation product accumulation, and sensory analysis were comparable in all the products. CONCLUSIONS The smoking time can be reduced due to the dark color of the hams covered with BOE film. The barrier properties of those films should be increased. Other parameters were comparable to plastic packaging. The films therefore have the potential to be used instead of plastic packaging in the meat industry. © 2020 Society of Chemical Industry.

Currently, animal proteins have a special role in the meat processing industry for meat products. A promising source of additional food protein production is natural collagen-containing raw materials, which can be used as part of protein-fat emulsions. The paper presents the results of research on nutritional and biological value of meat paste using new types of emulsions from collagen-containing raw materials, which have undergone technological treatment with bacteria p. Lactobacillus. To compare the data obtained, the studied samples of paste were compared with paste produces using emulsions from collagen-containing raw materials with no treatment, as well as subjected to hydrolysis. The results of determining indicators characterizing the nutritional and biological value of the developed paste are presented: content of protein, fat, nonessential and essential acids, polyunsaturated fatty acids and minerals. Development of a new method for preparing collagen-containing raw materials to be used in meat products will allow excluding vegetable protein from composition, as well as improving nutritional and biological value of the product. Collagen-containing raw materials used in meat paste will contribute to increase in use of biologically valuable secondary raw materials in meat-processing industry, as well as to expand of the range of high-quality food products of high nutritional and biological value for all age groups of population.

The article reveals some aspects of the influence of the duration of heat treatment and different types of functional mixtures on the functional and technological characteristics of broiler meat products at low temperature processing under the condition of target fermentation. Broiler meat has several advantages for use as a raw material in the production of Sous-Vide products. These advantages include high moisture content, high organoleptic characteristics, and relatively low cost of this raw material. However, there are a few disadvantages that complicate the processing of broiler chicken meat by Sous Vide technology. These include the high amount of moisture released during production and the low moisture-binding capacity of some batches of raw materials (often associated with excessive moisture or disruption of autolytic processes in broiler chickens). To achieve the desired consistency and high organoleptic characteristics, enzyme preparations are widely used in the meat processing industry. To eliminate these shortcomings, it is proposed to use functional mixtures, which are introduced into the raw meat by injection, as well as the use of targeted fermentation. During the experiment, 2 formulations of broiler chicken meat products were developed, which in turn were treated in two heat treatment regimens and treated with the enzyme transglutaminase. In the first batch of samples, an injection of 15% solution of a functional mixture based on citrates was used, while in the second batch - a similar level of injection of a mixture based on phosphates. Heat treatment was performed after pre-application in bags and vacuum at 62 ℃ for 120 and 140 min for different groups of samples. In all samples, the yield of the finished product, the relative weight of the separated moisture (broth) in the package, the values of pH, water holding and moisture-retaining capacities, as well as the total moisture content in the finished product were determined. According to the obtained results, the optimal is samples with the use of phosphates, and 120 minutes the duration treatment is 120 minutes.

In the meat industry, hydrocolloids and phosphates are used to improve the quality attributes of meat products. However, latest research results revealed that the usage of exopolysaccharide (EPS)-forming lactic acid bacteria (LAB), which are able to produce EPS in situ during processing could be an interesting alternative. The current review aims to give a better understanding of bacterial EPS production in food matrices with a special focus on meat products. This includes an introduction to microbial EPS production (homopolysaccharides as well as heteropolysaccharides) and an overview of parameters affecting EPS formation and yield depending on LAB used. This is followed by a summary of methods to detect and characterize EPS to facilitate a rational selection of starter cultures and fermentation conditions based on desired structure-function relationships in different food matrices. The mechanism of action of in situ generated EPS is then highlighted with an emphasis on different meat products. In the process, this review also highlights food additives currently used in meat production that could in the future be replaced by in situ EPS-forming LAB. ispartof: COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY vol:19 issue:6 pages:2932-2954 ispartof: location:United States status: published

Background Raw meat is one of the commonly consumed traditional diets in Ethiopia. However, unhygienic processing and distribution practices are risky for contamination of meat leading to human infection. This study was conducted to assess the presence of multi-drug resistant E. coli with special emphasis on E. coli O157:H7 from meat of cattle and swab samples at abattoir houses and butcher shops in Jimma town, Southwest district of Ethiopia. Methodology A cross-sectional descriptive study was conducted from April to July, 2018. The isolation and identification processes passed through enrichment of samples with modified tryptone soy broth (mTSB), streaked onto MacConkey agar and Cefixime-tellurite sorbitol MacConkey agar, biochemical testing (indole and TSI), followed by latex agglutination testing. Results Out of 505 samples, 102 (20.2%) and 27 (5.4%) were positive for E. coli and E. coli O157:H7, respectively. Of these, 55 (19.3%) and 47 (21.4%) of E. coli and 17 (6.0%) and 10 (4.5%) of E. coli O157:H7 were isolated from the abattoir and butcher shop samples, respectively. A significant difference in the occurrences was observed among sample sources. Antimicrobial susceptibility test results showed that, 92.2% to 96.1% of E. coli and 85.5% to 96.3% of E. coli O157:H7 were susceptible to third generation cephalosporin, ciprofloxacin, gentamycin, kanamycin, streptomycin, and chloramphenicol. About 91.2% and 97.1% of E. coli and 88.9% and 92.6% of E. coli 0157:H7 were resistant to ampicillin and erythromycin, respectively. A total of 57 (44.2%) E. coli and E. coli O157:H7 isolates were resistant to three or more classes of antibiotics. All abattoir and butcher shop workers did not have any formal education or training certificates on food safety, and unhygienic practices were also observed. Conclusion The presence of E. coli and E. coli O157:H7 including multi-drug resistant isolates in raw meat highlights how the current meat processing and distribution practice was unhygienic. Therefore, strategies in the prevention and control of food-borne infections that could be caused by multi-drug resistant strains will depend greatly on hygienic processing and distribution practices of meat.

Bacterial contamination on the outer grained hide of cattle serves as a significant challenge to the meat and hide processing plants regarding food safety. Cattle serves as a host to several bacteria including pathogens, which may become firmly lodged on a cattle’s hide during their lifespan and can facilitate cross contamination of the underlying meat and meat processing equipment in a slaughterhouse. In this study, the efficacy of sodium dichloroisocyanurate dehydrate (SDCIC), which belongs to N-halamine group of compounds is evaluated on inhibition of the natural-borne bacteria from the haired surface of cattle hide. Spray-washing with 0.25%, 0.50% and 0.75% aqueous SDCIC solutions reduced total aerobic bacteria on hide surface by 1.6 and 2.37 and 3.57 log CFU/50 cm2 respectively in 5 min. Similarly, in comparison with water spray, a 5 min treatment with 0.25, 0.50 and 0.75% SDCIC resulted in a reduction of Enterobacteriaceae (ENT) and related gram-negative bacilli (gnEB) counts by 1.2, 2.22 and 4.22 log CFU/50 cm2 respectively. The highest reduction of E.coli recovery of 1.48 log CFU/50 cm2 was obtained with a spray treatment of 0.75% SDCIC in 1 minute. Likewise, 0.75% SDCIC spray treatment resulted in 2.5 and 3.65 log CFU/50 cm2 reduction of Salmonella in 1 and 5 min, respectively in comparison to their respective controls. Leather from treated hides was tested to evaluate the effects of N-halamine solutions on structural, mechanical and subjective properties of produced leather in which no major detrimental impacts were found.

The aim of the work was to establish the frequency of detection of mold of raw smoked sausages and to evaluate the use of synthetic protective shrinks, which provide long-term storage of sausages. Nine batches (310 samples) of raw smoked sausages were examined, 487 crops were made on special media, 154 samples of sausages were examined according to physical and chemical parameters. We determined that an intensive contamination of their surface with micellar fungi during the sale of raw smoked sausages in retail trade is taking place: Penicillium (62.9%), Aspergillus (18.2%), Mucor (10.7%), Cladosprium (4.5%). We found that on the 5th day of their sale 4.57±1.64% of experimental sausage bulks are contaminated with micellar fungi but more massive and intensive development of micromycelia was found on the 20th day of sale (29.32±8.16% of cases). We proved that the use of protective coating/srink “Optiglianets” (Lofink Group, Ukraine), “CoatStar 110” (OJSC “Upakprint”, Russia), “Polisved1” (OJSC “Omega”, Russia), “Antibak Tauchmassa” (Company “Swed Holding”, Russia) and “Tauchmasse KP-15”, Italy), delayed up to 20 days the development of micellar fungi on the surface of prototypes of smoked sausages. We applied them in a thin layer (0.06-0.10 mm depending on the applied means) on the surface of sausage bulks and dried in drying chambers at temperature of 15.0±2.0 °C and 45 minutes exposure at a relative humidity of 70%. The weight loss of raw smoked sausages was the lowest with the use of protective coating “Optiglyanets” (Lofink Group, Ukraine) and “Polisved-1” (OJSC “Omega”, Russia). It was 14.5±0.8% and equal to 60% reduction compared to other protective coatings/shrinks implementation. 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In the field of agricultural and food processing, high power ultrasound (HPUS) is recognized as a green, physical and non-thermal technology in improving the safety and quality of foods. The functional properties of food proteins are responsible for texture, yield and organoleptic of food products which are the theoretical basis for food processing optimizing. HPUS treatment could provide the possibility for creating novel functional properties of new foods with desirable properties due to the modification of protein structure. In this article, an overview of the previous studies and recent progress of the relationship between structure modification and functional properties of food proteins using the HPUS technique were presented. The research results revealed that HPUS could significantly affect the conformation and structure of protein due to the cavitation effect resulting in the improvement of solubility, interfacial, viscosity, gelation and flavor binding properties of proteins. During meat processing, HPUS can modify the structure and thereby improve the functional properties of myofibrillar protein (MP), leading to the quality enhancement, low fat and/or salt products development and the shelf life extending. In view of this review, the recent findings of applications of HPUS in the production of meat products based on the modification of MP including curing, freezing/thawing and thermal processing have been summarized. Finally, the future considerations were presented in order to facilitate the progress of HPUS in meat industry and provided the suggestions based on the advanced protein modification by HPUS for the commercial utilization of HPUS in producing the innovative meat products.

The meat industry produces a lot of waste, which contains large amounts of the organics and nutrients. Animal by-products have potential for biomaterial extraction. The use of bio-material, which can be obtained from plant sources, microorganisms, agricultural, and animal waste are nowadays favored because of their compatible, cost-effective, and low-risk for removal of pollutants, compared to chemical and physical methods. In this study, a biopolymer from meat by-product extracted by methanol-chloroform and characterized by FTIR, GC–MS, HPLC, and SDS-PAGE analyzes. The extracted biomaterial was useful in water desalination by calcium carbonate precipitation and heavy metals removal, which was confirmed by FTIR and ICP analyzes. The extracted biomaterial also has antibacterial properties against Pseudomonas aeruginosa and Escherichia coli without toxicity to human blood cells, which can make it useful in industries such as its application in fish ponds.

Introduction. Extract of goji berries (Lycium Barbarum L.) and buckwheat flour (Fagopýrum esculéntum L.) possess antioxidant and antimicrobial properties. As a result, they can be used to improve traditional Kazakh horse-meat formulations to obtain functional cooked and smoked meat products. These natural biologically active substances can improve the oxidative stability of pigments, lipids, and proteins of finished products. The research objective was to assess the potential of goji extract and buckwheat flour as additives that can improve the oxidative stability and general quality of Kanagat, a national Kazakh cooked and smoked horse-meat product. Goji extract and buckwheat flour were used in two concentrations – 0.5% and 1.0%. Study objects and methods. The research featured sensory evaluation of taste, smell, color, determination of color parameters (L*, a*, b*), pH, free amine nitrogen, total carbonyl proteins, acid value, peroxide value and thiobarbituric acid reactive substances (TBARS), as well as a histological analysis. Results and discussion. When 1.0% of goji extract and 1.0% of buckwheat flour were added to the traditional formulation, it improved the oxidative stability and quality of the modified horse-meat product while preserving its sensory properties and colour parameters. A set of microstructural studies showed that the processing of meat products with 1.0% of goji extract and 1.0% of buckwheat flour had a destructive effect on most fibers. The affected fibers showed multiple decays of myofibrillar substance, which turned into a finegrained protein mass. The abovementioned concentration caused effective inhibition of hydrolytic changes, as well as oxidation of proteins and lipids. Conclusion. The new technology made it possible to produce a new national horse-meat product fortified with 1.0% of goji extract and 1.0% of buckwheat flour. The specified amount of biologically active additives improved the oxidative stability and quality of the product, while maintaining its sensory and color characteristics.

Plant extracts contain large amounts of bioactive compounds, mainly polyphenols. Polyphenols inhibit the growth of microorganisms, especially bacteria. Their mechanism of action is still not fully understood but may be related to their chemical structure. They can cause morphological changes in microorganisms, damage bacterial cell walls and influence biofilm formation. Polyphenols also influence protein biosynthesis, change metabolic processes in bacteria cells and inhibit ATP and DNA synthesis (suppressing DNA gyrase). Due to the antioxidant and antibacterial activity of phenolic compounds, plant extracts offer an alternative to chemical preservatives used in the meat industry, especially nitrates (III). They can inhibit the growth of spoilage and pathogenic microflora, suppress oxidation of meat ingredients (lipids and proteins) and prevent discoloration. In this paper, we describe the factors that influence the content of polyphenols in plants and plant extracts. We present the antimicrobial activities of plant extracts and their mechanisms of action, and discuss the effects of plant extracts on the shelf-life of meat and meat products.

Synthetic growth stimulants are widely used to get high productivity of animals. These preparations can accumulate in the meat and their residual quantities will adversely affect the health of consumers. The purpose of the work was to monitor the content of zeranol, growth stimulant of ruminants in beef which goes to meat processing enterprises of the Western region of Ukraine and to determine the effect of heat treatment on its quantity. It was found out that 29.8% of beef samples taken at meat processing enterprises contained a stimulant for the growth of ruminant zeranol. It was found that during the storage of beef samples frozen at a temperature of -18 °C with different content of zeranol there is a decrease in its number. The most intense process of destruction of zeranol occurred during the first month of storage, during this period of time the amount of zeranol is reduced by an average of 20%, regardless of the initial content. Within two months of storage of frozen beef, the content of zeranol decreases by 28.2 ±0.17%, and at the end of the sixth month its quantity decreases to 33.2 ±0.58%. It was also found that the dynamics of zeranol reducing in beef samples with large quantities (22.5 μg.kg-1) and small (2.3 μg.kg-1) were the same. It was set up that during 30 min of meat cooking there was a decrease in the content of zeranol 24.7 ±0.23% and 32.0 ±0.35% for 60 min, compared to its content in fresh meat. At the same time, when stored in the frozen state and subsequent cooking, the reduction of zeranol content in meat was 39.3 ±0.3%. Therefore, it is proposed to revise and amendments into the regulatory documents of Ukraine regarding the control and supervision of the presence of hormone (zeranol) residues in meat and meat products in order to prevent their sale and consumption by humans.

The current study was carried out to investigate the biokinetic model of Botryococcus sp. in removing NH4+ and PO43- from meat processing wastewater. The removal efficiency of NH4+ ranged from 89.7...

Aqueous extract of ginger (Zingiber ofcinale) was investigated for its effect as a natural tenderizing agent in thigh meat obtained from spent hens of commercial layer stock. Te thigh portion obtained after slaughter and dressing of spent hens were treated with five treatment solutions viz. 0.2% papain as positive control (T1), naive control without any treatment (T2); 2% Ginger Extract (T3); 4% Ginger Extract (T4); and 6% Ginger Extract (T5). The samples were kept for 24 hours at 4oC and then divided into two groups. One group of samples was analyzed for various physico-chemical, tenderizing and sensory parameters to ascertain the effect of the Ginger Extract. The results revealed that with increasing Ginger Extract level, there was an increase in pH, moisture content and water holding capacity while as decrease in hydroxyproline content and collagen content, thereby decreasing the toughness and thus desirably improving the tenderness of spent hen meat. Ginger Extract also improved sensory parameters like appearance, favor, tenderness, juiciness and overall acceptability scores. The other group of samples was cooked for 35 minutes in 1.5% salt solution. Ginger Extract increased the product yield, pH and moisture content and decreased the shear force value in cooked samples. Ginger Extract also imparted better appearance, color and tenderness scores in cooked samples. Although all levels of Ginger Extract improved the quality of meat, however, the best results were found for 6% Ginger Extract. It was concluded that ginger is a potential natural tenderizer and can thus be explored for its role in the meat processing industry to improve the overall quality of otherwise less valued tougher spent hen meat.

Fermented meat products represent an important segment of our alimentation. Obtaining these products is based on beneficial microorganism activity. In the case of traditional food products, these are commercial starters or autochthonous microflora. Fermentation of raw materials is mainly done by sugar metabolization of lactic acid bacteria (LAB). In addition, these microorganisms can have other beneficial properties too such as probiotic properties, antimicrobial compound production abilities, etc. In order to meet consumer demands, starter cultures are continuously developed to produce high-quality, healthy, and tasty products, thus contributing to guaranteeing microbiological safety and to improving one or more sensory characteristics, technological, nutritional, or health properties of the fermented products. The aim of our research is to determine the technological properties of autochthonous lactic acid bacteria originated from commercial fresh sausages in order to select and use them as potential starter cultures in the meat industry. In our work, we determined the relevant characteristics (such as salt tolerance, proteolytic activity, antimicrobial activity, and antibiotic resistance) of bacteria isolated from 16 fresh sausages. Based on our results, the studied bacterial isolates originated from sausages could be potentially used as autochthonous meat starter cultures.

State monitoring is carried out to verify compliance with the requirements of the legislation on safety and individual indicators of food quality by market operators who produce meat and meat products, store (wholesale bases) and sell (agro-markets, supermarkets, etc.). When establishing a food hazard, the following conditions are taken into account: the usual conditions of use of the food by the consumer, each stage of its production, processing and circulation; information provided to the consumer, including labeling, including end-of-use (use) information, and other publicly available information on the avoidance of adverse health effects, food-related or food category the possible short- or long-term impact of the food on the health of the person consuming it and on future generations; possible cumulative toxicity effect. Therefore, it is important to develop simple, rapid methods for determining the adulteration of beef, pork, mutton, and meat of goat when carrying out risk-oriented controls on the sale of chilled and chilled meat in agri-food markets under different storage and marketing conditions. The falsification of chilled meat of slaughtered animals with a solution of sodium bicarbonate for 3–4 days of sale at temperatures from 0 to 6 °C was established: in beef and meat of goat – a weakly positive reaction (up to 5%), in pork and mutton positive (5.1% and more). Also falsification of meat of slaughtered animals with a solution of sodium bicarbonate on 17–18 (for beef) and 13–14 days of realization at temperatures from 0 to minus 1 °C was established: in beef, pork and meat of goat – a weak positive reaction (up to 5%), positive in mutton (5.1% or more). The technique of establishing adulteration was based on the use of an alcoholic solution of chrome dark blue with a mass concentration of 0.5%, applied to the surface of the muscle tissue, and after 1–2 seconds, the presence of pale pink color was established – in the absence of meat processing with sodium bicarbonate or from light purple to dark purple in the presence of sodium bicarbonate depending on the amount of meat processed up to 5.0% and 5.1% or more. The results of the studies showed that the stability of the color intensity in the falsification of meat of slaughtered animals by treatment with sodium hydrogen carbonate was 99.9%. The influence of sodium bicarbonate on the quality and safety of meat of slaughtered animals for 3–4 days of realization at temperatures 0–6 °C and 17–18 (for beef) and 13–14 days of realization at temperatures from 0 to minus 1 °C has been established: the content of microorganisms in the superficial layers of muscle tissue decreased significantly (P < 0.01, P < 0.001), and slightly increased slightly in the deep layers (P < 0.05); the pH was significantly (P < 0.001) increased and ranged from 6.45 ± 0.023 to 6.72 ± 0.018; NMAOAM content tended to decrease, but the difference was not significant in the case of a weakly positive reaction (at temperatures of 0–6 °C in beef and meat of goat) and a slight decrease (P < 0.05) in pork and mutton (positive reaction); at temperatures from 0 to minus 1 °C, NMAOAM content tended to decrease, but a significant difference, except for beef (1.10 times, (P < 0.05), was not established: in pork – 1.11 and meat of goat – 1.4 times, where the indicators slightly exceeded the limits of the standards of quality meat (103 CFU/g), respectively – (9.89 ± 2.65) ×102, (10.03 ± 3.62) ×102 and (10.4 ± 2.29) × 102 CFU/g, while in mutton (positive reaction) it decreased significantly by 1.05 times and was within the limits – 9.87 ± 0.27×102 CFU/g.

There is a continued need to develop improved rapid methods for detection of foodborne pathogens. Rapid and sensitive methods for enumeration of Listeria monocytogenes are important for microbiological food safety testing purpose. The aim of this project was to evaluate a commercial loop-mediated isothermal amplification (LAMP) based system with bioluminescence, named as 3M™ Molecular Detection Assay (MDA), was validated for the detection of L. monocytogenes in food products with a standard GOST 32031-2012 method as reference. The results of this study revealed that a commercial LAMP-based method performed equally effective compared with method, showing from 94% to 100% specificity and sensitivity, respectively. The LAMP-based method was shown to be rapid and reliable detection technique for L. monocytogenes present at low numbers (10 CFU.g-1) on raw meat and meat products and can be applicable in meat industry. Thus, compared with the microbiological method based GOST 32031-2012, the LAMP assay is a relatively rapid and highly sensitive method for detecting L. monocytogenes and will facilitate the surveillance for contamination of L. monocytogenes in food. The 3M MDS result and culture-based detection (GOST 32031-2012) did not differ significantly (p >0.05) regarding the number of positive samples.

In this study, an advanced oxidation process of Fenton Process was applied to meat prosessing wastewater sludge for the purpose of sludge disintegration before anaerobic digestion. Fenton Process was applied to the meat processing wastewater sludge samples were taken from a treatment plant located in Denizli, Turkey. In the first stage of the study, experiments were carried out to optimize the process conditions in terms of disintegration using Box-Wilson Statistical Design. Ferrous iron and hydrogen peroxide concentrations were chosen as process variable and disintegration degree parameter based on soluble chemical oxygen demand calculations used as process response. 13% of disintegration degree was obtained at 90 gH2O2/ kg Dried Solids and 3 g Fe(III)/kg Dried Solids. After optimization studies for disintegration, sludge digestion studies were carried out using Biochemical Methane Potential (BMP) Test. BMP test results showed that Fenton Process can be used as a sludge disintegration purpose and application of Fenton Process before anaerobic digestion causes 33% higher methane gas production comparing the non-pretreated sludge.

The application of various functional protein additives in the meat industry is still increasing. Soy, milk and egg white proteins are applied to various types of meat product to improve their structural properties or as good substitutes for meat and fat components. It is common practice to add these preparations to reduce production costs. New methods are needed for reliable detection of small amounts of these additives in complex and processed food matrices regarding the potential for food adulteration and allergen detection. In this work, a mass spectrometry method was implemented for the detection of soy (Glycine max), milk (Bos taurus) and egg white (Gallus gallus) in smoked and cooked pork sausages. Specific peptide markers for glycinin and β-conglycinin, α-S1-casein, β-lactoglobulin, ovotransferrin and lysozyme C were monitored in the sausage samples. The proteins were detectable at the low concentration of 0.53% (w/w) of a given additive, which corresponds to a limit of detection of 0.14% for milk proteins and 0.45% for soy and egg white proteins in the final meat product. The results were validated by the use of synthetic peptides labelled with stable isotopes. Considering the universal nature of the presented peptide markers, the method has the potential to be implemented for other complex and processed foodstuffs for authenticity purposes.

Meat samples (Longissimus dorsi) were processed using an ultrasonic (US) probe system (20 kHz) and a US bath (33 kHz), in brine solutions of 15% NaCl or NaCl/KCl. Selected quality parameters, namely hardness (Warner&ndash, Bratzler shear force, WBSF), secondary lipid oxidation products (thiobarbituric acid reactive substances, TBARs) and total colour difference (TCD) were analysed at day 0 and day 60. Inoculated E. coli and L. innocua cells, total viable counts and lactic acid bacteria were also monitored for 60 days on meat stored at 4 and 10 &deg, C. US brining could achieve a 25% sodium reduction in a shorter processing time. No changes were observed for WBSF and TBARs values, noticeable colour differences (&Delta, &Epsilon, &gt, 5) were measured in US-treated samples at the end of storage. Whilst no differences were observed in the levels of inoculated and spoilage bacteria on the meat surface, a significant reduction in E. coli in the brine subjected to US treatment indicates the potential of US as a hurdle technology to prevent cross contamination during meat processing. These results suggest that US processing, in combination with KCl, could assist current sodium reduction strategies improving processing time. In addition, the potential effects for decontamination of brining tanks increasing the shelf-life of the brine and preventing processing losses are highlighted.

Microbial contamination is one of the most important factors that affects the safety and shelf life of meat and meat products. Effective decontamination is paramount to consumers for the safety. Cold plasma as an effective and rapid non-thermal decontamination treatment technology, the rapid microbial inactivation achieved in the food industry has aroused the researcher’s interest. This review mainly summarizes the published researches on the application of cold plasma in fresh animal and poultry meat and meat products. The bactericidal effect of cold plasma on microorganisms and the impact on product quality for animal, poultry, and eggs were discussed, respectively. Application of cold plasma during meat processing was also discussed in this review. Compared with traditional thermal technologies, cold plasma has unique advantages, such as non-thermal, design versatility, economical, and friendly to environment. Cold plasma technology is expected to be widely used in the meat industry in the near future, and further study is still needed to get its potential.

The present study analyzed the influence of horse age, substances used for marinating, and frozen storage on the quality of horse meat. It was conducted on the samples of the longest thoracic muscle, obtained from 12 carcasses of horses (aged 4–7 and 8–12 years). Among the analyzed samples, a higher fat content (p &lt, 0.05) was found in the meat obtained from the carcasses of older horses. The pH value of the meat samples was influenced by the treatment applied (p &lt, 0.05). Of the substances used for marinating, malic acid caused a decrease in the pH of the meat obtained from young horses (p &lt, 0.05). A similar effect was observed with the addition of phosphates to malic acid-marinated meat. On the other hand, the use of phosphates for marinating resulted in an increase in the pH of the meat obtained from older horses (p &lt, 0.05). The substances used for marinating the horse meat did not significantly affect the reduction in cutting force values. Furthermore, the values of shear force, hardness, stiffness, gumminess, and chewiness of the meat increased with horse age (p &lt, 0.05). An influence on the color parameters a* and b* of the meat was found for the interaction between age, storage period, and the type of treatment (p &lt, 0.05). The use of lactic acid and malic acid for marinating the meat of young horses caused a decrease in the proportion of red color (4.67 and 3.43) and an increase in the proportion of yellow color (3.81 and 1.71), especially after 3 months of freezer storage. All the substances used for marinating (except for phosphates) were associated with higher (p &lt, 0.05) thermal and forced drips of meat from the carcasses of both young and older horses during each storage period, in comparison to the control. The interaction between age and the type of treatment had an influence on the tenderness and juiciness of the horse meat (p &lt, 0.05). In sensory evaluation, it was noted that the interaction between age and the treatment procedure influenced the tenderness and juiciness of the meat samples (p &lt, 0.05). There is still a need for further research to increase knowledge regarding how to improve the quality of horse meat, and ultimately increase the demand from consumers and meat processing plants.

Poultry waste is increasingly used as a substrate for the methane fermentation process in a biogas plant. However, not all waste materials processed in the meat industry meet the criteria for optimal process management and cost-effective methane efficiency. An example may be centrifuged biological sludge, etc. Treatment of such material used as a substrate by introducing new metabolically and enzymatically active strains of bacteria could be beneficial for the fermentation process in a biogas plant and provide increased energy efficiency. The aim of the study was to compare the amount and quality of biogas obtained from biological sludge from the processing of poultry vaccinated with metabolically diversified bacterial inoculum after initial incubation of the batch before the actual process in a biofermenter. Laboratory tests were carried out in accordance with the guidelines contained in the DIN 38 414-S8 and VDI 4630 standards. Based on the obtained results, it was found that the optimized biological sludge can be used as a substrate in the methane fermentation process in a biogas plant. The material processed by the combination of bacterial strains marked with the symbols A/C, E/G, and F/H showed a significantly increased efficiency of biogas, including methane, compared to the non-grafted material. This is a good predictor for industrial applications, process feasibility, economic viability, and environmental sustainability that should be compiled based on the results obtained from this study.

Iron-porphyrin is a very important substance in organisms, especially in animals. It is not only the source of iron in human body, but is also the catalytic center of many reactions. Previous studies suggested that adequate intake of iron was important for the health of human, especially for children and pregnant women. However, associated diseases caused by iron over-intake and excessive meat consumption suggested its potential harmfulness for human health. During meat processing, Iron-porphyrin will cause the oxidation of proteins and fatty acids. In the gastrointestinal tract, iron-porphyrin can induce the production of malondialdehyde, fats oxidation, and indirectly cause oxidation of amino acids and nitrates etc. Iron-porphyrin enters the intestinal tract and disturbs the balance of intestinal flora. Finally, some common measures for inhibiting its activity are introduced, including the use of chelating agent, antioxidants, competitive inhibitor, etc., as well as give the hypothesis that sodium chloride increases the catalytic activity of iron-porphyrin. The purpose of this review is to present an overview of current knowledge about the changes of iron-porphyrin in the whole technico- and gastrointesto- processing axis and to provide ideas for further research in meat nutrition.

In meat processing, changes in the myofibrillar protein (MP) structure can affect the quality of meat products. High hydrostatic pressure (HHP) has been widely utilized to change the conformational structure (secondary, tertiary and quaternary structure) of MP so as to improve the quality of meat products. However, a systematic summary of the relationship between the conformational structure (secondary and tertiary structure) changes in MP, gel properties and product quality under HHP is lacking. Hence, this review provides a comprehensive summary of the changes in the conformational structure and gel properties of MP under HHP and discusses the mechanism based on previous studies and recent progress. The relationship between the spatial structure of MP and meat texture under HHP is also explored. Finally, we discuss considerations regarding ways to make HHP an effective strategy in future meat manufacturing.

Simple Summary Animal production generates emissions of gaseous pollutants to the environment. Natural sorbents are used to reduce emissions increase feed safety and reduce the impact of farms on the environment. Therefore, the aim of the study was to assess the effect of three natural sorbents added to the diet of pigs on the composition and physicochemical properties of two skeletal muscles (Longissimus lumborum and semimembranosus) of crossbred pigs. The experiment was carried out on a farm in two production cycles. The animals received feed supplemented with three different natural sorbents. The results indicate that the sorbents used in the diet of pigs have no negative effect on the physicochemical properties of meat or its use as case-ready meat or material for processing. Abstract The effect of three natural sorbents added to the diet of pigs on the composition and physicochemical properties of two skeletal muscles—the musculus Longissimus lumborum (MLL) and musculus semimembranosus (MSM) of crossbred pigs were evaluated. The experiment was carried out on a farm in two production cycles. The addition of biochar (trial 1) significantly influenced instrumental color parameters, shear force and energy, and the oxidative stability of the skeletal muscles, while the proximate composition, pH, texture, and water-holding capacity (WHC) parameters did not differ significantly between groups in either of the two muscles. Similarly, no statistical differences were noted in the proximate chemical composition, texture parameters, or WHC of the meat in trial 2. The addition of both sorbents was associated with a significantly (p ≤ 0.01) lower content of haem pigments in the MLL and MSM, which was accompanied by a significantly (p ≤ 0.05) higher lightness (L*). Moreover, the MLL muscle of the pigs had higher oxidative stability, as well as lower drip loss (DL). In turn, the MSM of pigs had a significantly lower pH compared to the control group, however, the ultimate pH (48 h) in all groups was within the acceptable range (5.50–5.80). Summing up, the sorbents used are a safe ingredient in the diet of pigs, however, there is a need to continue and strengthen this line of research, including the relationships linking the future production goals of pig farming and processing potential in the meat industry with current climate policy.

Reformulation approaches in the meat industry are required to promote nutritional improvement, health functionality, and reduce environmental impact. A relevant approach among these is to reduce the amount of meat in meat products. Reduced-meat products should maintain or improve the sensory characteristics and nutritive value compared to conventional meat products. Among meat products, heat-treated emulsion-meat sausages are widely consumed and especially suitable for reformulation approaches. Due to its high protein content, with high functionally and biological value, pulse flour has a high potential to be used as meat replacer. Most studies regarding the replacement of meat with pulses have been made on fresh meat preparations where amounts of up to 15% of pulse flour did not negatively affect sensory quality while increased yield and firmness. However, studies using pulse flour in emulsion-type sausages are scarce. Further research is warranted to optimize the reformulation of these meat products using flour pulses. The topics to be addressed are the following: effects of pulse type, pulse pretreatments, such as soaking or germination, pulse flour treatments before incorporation into the meat mix, combination of pulses with other proper ingredients, and heat treatment intensity on the pulse antinutrient inactivation and the technological and edible quality traits of the pulse-containing sausages.

The packaging protects food from actions of external agents, from alterations and contaminations, in addition to adulteration. Edible films are structures produced from biopolymers, which can replace non-biodegradable packaging. These films have been formulated with naturally polymers of polysaccharides, lipids and proteins, isolated or combined with each other. Edible films produced with polysaccharides and proteins are transparent and flexible, although proteins films are less resistant. They act as a barrier, protecting food and increasing shelf life. Additionally, they can carry antimicrobial and antioxidant compounds, being called active films. The antioxidant power is proportional to the amount of the compound added. Natural extracts such as green tea, cloves, ginger and others can be incorporated into the films, wich could improving the mechanical properties of the films and the characteristics of the food. The use of active edible films has been evaluated in the meat industry as an alternative packaging. This review aims to address the use of edible films added with vegetable compounds, with antimicrobial and antioxidant activity, applied to meat products. El envase protege los alimentos de la acción de agentes externos, de cambios y contaminación, además de la adulteración. Las películas comestibles son estructuras producidas a partir de biopolímeros, que pueden reemplazar los envases no biodegradables. Estas películas se han formulado con polímeros naturales de polisacáridos, lípidos y proteínas, aislados o combinados entre sí. Las películas comestibles producidas con polisacáridos y proteínas son transparentes y flexibles, aunque las proteínas son menos resistentes. Actúan como una barrera, protegiendo los alimentos y aumentando la vida útil. Además, pueden transportar compuestos antimicrobianos y antioxidantes, que se denominan películas activas. El poder antioxidante es proporcional a la cantidad de compuesto agregado. Se pueden incorporar a las películas extractos naturales como té verde, clavo, jengibre y otros, lo que puede mejorar las propiedades mecánicas de las películas y las características del alimento. El uso de películas comestibles activas se ha evaluado en la industria cárnica como envasado alternativo. Esta revisión tiene como objetivo abordar el uso de películas comestibles añadidas con compuestos vegetales, con actividad antimicrobiana y antioxidante, aplicadas a productos cárnicos. A embalagem protege o alimento de ação de agentes externos, de alterações e contaminações, além de adulterações. Filmes comestíveis são estruturas produzidas a partir de biopolímeros, que podem substituir as embalagens não biodegradáveis. Esses filmes têm sido formulados com polímeros de ocorrência natural de polissacarídeos, lipídeos e proteínas, isolados ou combinados entre si. Filmes comestíveis produzidos com polissacarídeos e proteínas são transparentes e flexíveis, embora os proteicos sejam menos resistentes. Agem como barreira, protegendo o alimento e aumentando a vida de prateleira. Adicionalmente, podem carrear compostos antimicrobianos e antioxidantes, sendo denominados filmes ativos. O poder antioxidante é proporcional à quantidade do composto adicionado. Extratos naturais como chá verde, cravo, gengibre e outros podem ser incorporados aos filmes, que podem melhorar as propriedades mecânicas dos filmes e as características do alimento. A utilização de filmes comestíveis ativos têm sido avaliados na indústria de cárneos como embalagem alternativa. Esta revisão, tem por objetivo abordar a utilização de filmes comestíveis adicionados de compostos vegetais, com atividade antimicrobiana e antioxidante, aplicados em produtos cárneos.

Musculoskeletal disorders of the wrist are common in the meat industry. A proof of concept of a physical human-robot interaction (pHRI)-based assistive strategy for an industrial meat cutting system is demonstrated which can be transferred to an exoskeleton later. We discuss how a robot can assist a human in pHRI, specifically in the context of an industrial project i.e for the meat cutting industry. We developed an impedance control-based system that enables a KUKA LWR robot to provide assistive forces to a professional butcher while simultaneously allowing motion of the knife (tool) in all degrees of freedom. We developed two assistive strategies—a force amplification strategy and an intent prediction strategy—and integrated them into an impedance controller This research received funding from the French government research program Investissements d‘Avenir through the UMTs ACTIA Mécarnéo-AgRobErgo and the project Exoscarne (Call P3A-ICF2A-2I2A, FranceAgriMer) and from the European Union’s Horizon 2020 research and innovation programme under grant agreement nº 869855 (SoftManBot project) SI

Microstructural factors associated with surface iridescence in cooked, cured pork products were investigated. Meat iridescence is a commonly observed physical phenomenon in raw meat and meat products that consist of intact muscle tissue. Since the purchase decision of consumers is mainly driven by the first impression of meat color and appearance, products showing colorful iridescence may be rejected. Four different muscles (RF: M. rectus femoris, BF: M. biceps femoris, ST: M. semitendinosus, and LD: M. longissimus thoracis et lumborum) were brine-injected, cooked, sliced, and iridescence was evaluated by digital image analysis and sensory analysis. Sarcomere lengths, fiber diameters, and surface microstructure were analyzed in iridescent and noniridescent sections. Highest iridescence extent by image analysis was found in LD (37.3 ± 16.4%), and highest overall iridescence score (extent and intensity, 6.11 ± 1.78) was observed in BF. Sarcomere lengths did not differ significantly between iridescent (1.05 ± 0.09 µm LD) and noniridescent areas (1.08 ± 0.94 µm LD) within muscles (p > 0.05). Iridescent sections showed smooth and ordered surface structures with cross-sectioned myofibers, whereas in noniridescent sections, surfaces were more unstructured and myofibers obliquely cut. The results of the study indicate that the sarcomere length and fiber diameters may thus be only of minor importance for the explanation of meat iridescence in cooked meat products and are rather related to multiple scattering and absorption effects on smaller structural entities such as the myofilament lattice or larger entities such as fiber bundles. PRACTICAL APPLICATION: Iridescence can be a problem for the meat industry due to consumers concerns about green-iridescent colors in meat. The underlying mechanisms and structures have not yet been fully clarified, and thus no practical solutions to eliminate iridescence have been found so far. This research presents new insights into the structural attributes that are interrelated with meat iridescence and shows that iridescence is rather influenced by cutting angle of muscle fibers and surface homogeneity than by muscle fiber diameters or sarcomere lengths. This should be considered by the industry when seeking for ways to reduce the potential problem of iridescence.

Staphylococcus aureus is Gram-positive bacterium commonly associated with food poisoning diseases. This pathogen is responsible for food-borne illnesses outbreaks associated with the consumption of food especially meat products. The aims of this study were to evaluate the ability of Staphylococcus aureus recovered from food contact surfaces in meat processing environment of Egypt for biofilm formation and to test their susceptibility to the commercially used sanitizers including Quaternary ammonium compounds (QACs, 200ppm), Sodium hypochlorite (200ppm) and Iodine (2.5%). The ability of isolates for biofilm formation and their sensitivity to sanitizers were investigated in 96-well flat bottom microtiter plates. Twelve strains were obtained from 120 food contact surfaces (FCS) using standard microbiological techniques. All isolated strains 100% (12/12) manifested high ability to form biofilm, which classified as strong type. Further, the application of QACs, Sodium hypochlorite and Iodine generated a reduction of (76.77%), (71.38%) and (15.84%) in the biofilm formation, respectively. Conclusively, all sanitizers were not 100% efficient in removing the biofilm formed by isolated S. aureus on polypropylene. Therefore, vigilant food safety practices need to be implemented in the meat processing environment, at meat processing plant and food services establishments, especially for FCS to prevent foodborne infections and intoxications due to S. aureus contamination from biofilms.

O objetivo deste trabalho foi avaliar os aspectos qualitativos de hambúrgueres e linguiças feitos com cortes menos nobres da carcaça de cordeiros alimentados com grãos de girassol e vitamina E. A carne do pescoço e das costelas de 32 cordeiros Ile de France abatidos aos 32 kg de peso corporal foi utilizada na confecção dos processados. Os cordeiros foram divididos em quatro tratamentos com oito animais em cada: C – cana-de-açúcar + concentrado; CG – cana-de-açúcar + concentrado com grãos de girassol; CE – cana-de-açúcar + concentrado com 1000 mg vitamina E/ kg de matéria seca (MS) da dieta e CGE – cana-de-açúcar + concentrado com grãos de girassol e 1000 mg vitamina E/ kg de MS da dieta. Observou-se interação entre grãos de girassol e vitamina E (P

This study investigated the effect of the combination of basic electrolysed water (BEW) and slightly acid electrolysed water (SAEW) with ultrasound (US) for cleaning and sanitation of the knives used in slaughtering processes. The knives were sonicated in a US bath using two modes of operation (normal and sweep) in two steps: (i) 5 min with BEW and (ii) 10 min with SAEW at 35 °C. The microbiological counts and the possible changes in the physical structure of the knives were evaluated. The association BEW + SAEW + US, in the sweep mode, provided lower mesophilic, enterobacterial, Staphylococcus aureus and yeast counts when compared to the values recommended by the international legislation. In addition, these conditions removed all organic residues from the knife blades and promoted the highest migration rate of the residues to the US water bath (12.35 mg/L·min), without modifications in the knife blades. Thus, cleaning and sanitation of knives was feasible with the association of BEW + SAEW + US, which could be a useful alternative for the meat industry.

Background A great number of plants have been studied in recent times in order to identify and isolate new components with nutraceutical properties. In addition, there is a growing interest in the re-valorization of industrial byproducts as a source of bioactive molecules. Scope and approach The present study highlights the potential of pine bark extract as a source of antioxidant and treatment diseases. Pine bark has a great content of bioactive molecules including polymeric flavanols, tannic acid, flavonoids and other phenolic acids such as caffeic or protocatechuic acid. Herein, we summarize the most recent uses of pine bark extracts in the food industry and for biomedical purposes. Key findings and conclusions Regarding the food industry, pine bark extracts have been successfully used as antioxidants in the meat industry and have been proposed as interesting additives for the juice industry and Songzhen noodles production. On the other hand, different Pinus spp bark extracts have been tested against a wide range of disorders -cancer, diabetes mellitus, hypertension, atherosclerosis, neuroprotective effect and other oxidative stress and inflammatory-related diseases. Both in vitro and in vivo assays suggest a promising future for pine as an adjuvant therapy.

Porcine plasma protein (PPP), a by-product of the meat industry, is considered for the development of eco-friendly superabsorbent materials due to its relatively high content in polar amino acids. Using glycerol (Gly) as plasticizer, different PPP/Gly ratios (50/50, 55/45, 60/40) were injection moulded at different moulding temperatures (60-120 °C). It is noteworthy that all bioplastics produced might be defined as superabsorbent materials, except those processed at higher moulding temperatures (100, 120 °C). Bioplastics moulded for 150 s at lower temperatures (60 °C) and using a holding pressure of 500 bar displayed the highest water uptake capacity (∼2,530%). Moreover, these processing conditions also resulted in lower thermomechanical properties. PPP represents an attractive and economic bioresource for the production of biodegradable superabsorbent matrices, which could represent an alternative to the extensively used synthetic materials.