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The transformation of biomass derived platform molecules is an interesting approach to produce valuable chemicals from biomass. In addition, process intensification by reducing the number of steps for final chemicals production by performing cascade-type catalytic reactions in one-pot mode is largely desirable in a biorefinery facility. In this review the possibilities of valorization of representative platform molecules such as sugars, itaconic and levulinic acids and furanic aldehydes through one-pot cascade processes using mono and multifunctional heterogeneous catalysts are illustrated through selected examples.

With advances in technology, the generation of electrical energy through the harvesting of energies dissipated in the form of mechanical vibration, known as power harvesting, has received increasing attention in recent decades. It is undoubtedly an interesting means to power systems with low energy consumption. This research aims to evaluate an energy generation system based on the piezoelectric effect activated by mechanical excitation and develop a system capable of powering devices and sensors for temperature monitoring in a practical situation, such as in an engine room, aiming to ensure its safe operation. Two transducers subjected to vibrational excitation were evaluated, and then an energy generation system using a buck DC-DC converter circuit was assessed. The transducer was connected to the input of the board, the microcontroller to the output, and the LM35 temperature sensor along with the battery was used to ensure the circuit’s autonomy. Additionally, the Attiny85 microcontroller was programmed to perform temperature monitoring tasks in a continuous low-energy-consumption mode. The obtained spectral analysis results showed a maximum generation power of 8.88 mW for the PZT-5H transducer and 3.3 mW for the P5-13B transducer. The use of LTC3588-1 increased the autonomy of the monitoring system by 64.3% and reduced the system’s usage time in cases of temperature anomalies by 50%.

During the desalting of salted cod, significant textural, histological, and biochemical changes occur. Understanding these changes is crucial for enhancing the preservation and extending the shelf life of desalted cod. This study aimed to investigate the physicochemical quality parameters and enzymatic activities during the desalting process of cod (16 h at 4 and 20 °C) and to extend the shelf life of desalted cod through high-pressure processing (HPP) at 400 and 550 MPa for 5 min. During desalting, a correlation was noted between the pH and trimethylamine content in samples desalted at 20 °C, with both parameters increasing in the initial 4 h and stabilizing thereafter. The soluble protein in cod muscle decreased over desalting time, as it dissolved into the desalting water. Enzymatic activity showed a decline in cathepsins (B and D) and acid phosphatase throughout desalting, whereas lipase activity increased, particularly at 20 °C. HPP effectively extended the shelf life of desalted cod by controlling endogenous microbial growth, enabling an extension to 14–21 days compared to the 7 days observed in untreated control samples. This study highlights quality changes during desalting, with lesser effects at lower temperatures. Subsequent HPP improved the microbiological quality of desalted cod during refrigerated storage.

This research is a large study over an extended period of time involving hundreds of general chemistry students and multiple instructors at the US Naval Academy. The purpose was to investigate whether a combination of clicker content and confidence questions would improve the performance and metacognition of students of different achievement groups (top, middle, bottom). Results showed no statistical difference in exam achievement between students who used clickers and those who did not when other active teaching methods were employed in both groups. Within the clicker participants, statistical differences in confidence on clicker questions were found by student achievement group, question type (algorithmic and conceptual), and question difficulty for both semesters of general chemistry. Students in the top achievement group were the most confident. All students were more confident on algorithmic and easy questions, though a significant interaction was found between question type and difficulty in the second semester. Metacognition was measured by calibration (confidence when the answer is corrector lack of confidence when incorrect). Overall, students of all achievement groups were well calibrated with students in the top group being confident in correct answers and students in the bottom group nonconfident within correct answers. Students in the middle group displayed calibration between those of the top and bottom groups. Evidence of calibration for middle and bottom students may indicate the beginning of metacognitive awareness. A positive trend was found in student perceived value of confidence questions over the two semesters of general chemistry.

This work provides significant insight into the molecular structure of alizarin lake pigments used by artists in the past. To characterize two red powders, lakes 1 and 2, obtained by complexation of 1,2-dihydroxy anthraquinone (alizarin) with Al3+, a multi-analytical approach was designed based on solid and liquid state Nuclear Magnetic Resonance Spectroscopy (NMR), Fourier-Transform Infrared Spectroscopy (FTIR), Mass Spectrometry (MS) and Density Functional Theory (DFT) calculations. Lake 1 was synthesized according to literature and compared with lake 2, a reproduction of an artist’s pigment. FTIR showed Al3+ coordinated to oxygens in C1 and C9, and that in lake 2 the -OH groups in C2 are protonated, being responsible for its low solubility. 1H-NMR proved that lake 2 is formed by two tautomers [Al(Aliz-2-H-)2(OH)(H2O)] and [Al(Aliz-10-H-)2(OH)(H2O)], the latter being the major species. SS-NMR was the only technique that got insight into the Al3+ coordination, octahedral for both lakes. It confirmed the existence of two species in lake 2, in a 5:1 ratio. Both are amorphous “open structures”, resulting in fewer constraints for the ligands and in a large variety of geometries. SS-NMR allowed the analysis of the red pigments without preparation, which is a unique advantage for their study in artworks.

Introduction: The increasing global pressure to explore alternative protein sources derived from animal by-products has opened-up opportunities, but it has also created the need to assess their compliance with labelling statements, to ensure consumer’s trust in the composition of both feed and food products. Assessing the authenticity of highly processed animal by-products, particularly within the rapidly expanding Halal food market, presents a significant challenge due to the lack of robust and standardized methodologies. However, the success of DNA based authenticity system is highly dependent on the extracted DNA quantity, quality, and purity ratios from heterogeneous matrices.Material and methods: In this work, nine DNA extraction methods were tested on selected processed animal by-products with high-value and interest for the feed industry: meals from poultry meat, blood and feather, and hydrolysates from swine meat and bone, fish, and black soldier fly. The proposed DNA extraction methods are developed to specifically target swine-specific mitochondrial region, as a case study.Results and discussion: Both the conventional CTAB method and the commercial kits, specifically Invisorb® Spin Tissue Mini and NucleoSpin™ Food, demonstrated superior extraction efficiency and quality ratios. Nevertheless, commercial kits enabled faster detection in comparison to the conventional methods. The absence of swine DNA was successfully validated and confirmed in all animal meals and hydrolysates that did not contain swine in their composition beforehand, demonstrating their compliance with the Halal market requirements.

A proposal to control the structural pounding hazard imposed on multistory reinforced concrete (RC) structures is presented. The main goal is to guarantee the seismic performance of a structure with an acceptable (predefined) risk-targeted parameter without the need to eliminate structural pounding collisions. The key target parameters of this study are the annual probability of exceeding an engineering demand parameter (EDP) capacity level and the separation distance dg between adjacent structures. In this direction, a method that ensures the performance level of critical EDPs due to structural pounding conditions is proposed. The new method involves two decision frameworks that define (a) the optimal separation gap distance dg,minPt at a targeted value of pounding risk (probability per year) Pt (Decision A) and (b) the minimum acceptable structural pounding risk Pmindg,t at a targeted value of separation gap distance dg,t (Decision B). The demand parameters that are incorporated in the proposed method are the peak relative displacement δmax at the top level of colliding without considering pounding conditions and any other critical EDP due to the structural pounding effect. The overall method is based on two distinct acceptable performance objectives, the POs-δmax and the POs-EDP, defined as a function of P vs. dg. For this purpose, a seismic hazard curve compatible with Eurocode’s 8 hazard zone is adopted, and the corresponding demand hazard curves of δmax and EDP are developed. The proposed method is implemented to study the floor-to-floor structural pounding hazard of an eight-story RC frame taking into account different risk-targeted scenarios. The results show that the seismic risk (probability per year) of exceeding the EDP’s capacity level is significantly increased due to structural pounding in comparison to the case of no pounding. Calibration of the structural pounding risk can be obtained by adjusting the separation gap distance dg between the adjacent structures based on the acceptable POs. The POs-δmax is not always an accurate criterion for verifying the capacity level of the critical EDP. Finally, with the proposed method, a variety of POs-EDPs can be used to control the structural pounding risk in terms of dg,minPt and/or Pmindg,t.

Mutations to oncogenic protein KRAS are responsible for some of the deadliest cancers, and KRAS is thus a key target for new antitumour agents. Here, a short bis-histidine peptide derived from the αH helix of the cofactor SOS1 is designed and shown to reversibly bind to KRAS with high affinity upon coordination to Pd(II), inhibiting KRAS-activated pathways in live cells.

Europe faces many problems connected to ageing infrastructure which was built in the second half of the 20th century. Bridges are one of the crucial elements of these infrastructures. In recent years, European countries have witnessed many failures of bridges across the continent. For example, the collapse of Viadotto Polcevera in Genoa caught the attention of society regarding its tragic consequences. Therefore, engineers must deal with the assessment of existing bridges which is essential for proper decision-making. Condition rating systems for bridges vary from country to country. Consequently, these differences in the methodology can lead to different conclusions related to the future service of assessed structures. For these reasons, this paper briefly describes condition rating systems for road bridges in Italy, Slovakia, and Portugal and defines the differences in the methodology. Subsequently, the obtained conclusions are compared and discussed. The aim of the paper is to encourage standardization in the assessment of bridge health conditions within European countries, highlighting the differences in the current systems adopted by various countries.

Water is the most important resource for all kind forms of live. It is a vital resource distributed unequally across different regions of the globe, with populations already living with water scarcity, a situation that is spreading due to the impact of climate change. The reversal of this tendency and the mitigation of its disastrous consequences is a global challenge posed to Humanity, with the scientific community assuming a major obligation for providing solutions based on scientific knowledge. This article reviews literature concerning the development of nanomaterials for water purification technologies, including collaborative scientific research carried out in our laboratory (nanoLAB@UA) framed by the general activities carried out at the CICECO-Aveiro Institute of Materials. Our research carried out in this specific context has been mainly focused on the synthesis and surface chemical modification of nanomaterials, typically of a colloidal nature, as well as on the evaluation of the relevant properties that arise from the envisaged applications of the materials. As such, the research reviewed here has been guided along three thematic lines: 1) magnetic nanosorbents for water treatment technologies, namely by using biocomposites and graphite-like nanoplatelets; 2) nanocomposites for photocatalysis (e.g., TiO2/Fe3O4 and POM supported graphene oxide photocatalysts; photoactive membranes) and 3) nanostructured substrates for contaminant detection using surface enhanced Raman scattering (SERS), namely polymers loaded with Ag/Au colloids and magneto-plasmonic nanostructures. This research is motivated by the firm believe that these nanomaterials have potential for contributing to the solution of environmental problems and, conversely, will not be part of the problem. Therefore, assessment of the impact of nanoengineered materials on eco-systems is important and research in this area has also been developed by collaborative projects involving experts in nanotoxicity. The above topics are reviewed here by presenting a brief conceptual framework together with illustrative case studies, in some cases with original research results, mainly focusing on the chemistry of the nanomaterials investigated for target applications. Finally, near-future developments in this research area are put in perspective, forecasting realistic solutions for the application of colloidal nanoparticles in water cleaning technologies.

On the basis of the results of two prior studies at the US Naval Academy (USNA), which described the choice of study resources and the self-reported learning approaches of students of differing achievement levels, the current investigation examines how students of differing achievement levels in general chemistry actually solve multiple-choice questions. A think-aloud protocol was selected as the vehicle for this investigation. This research utilized and compared the correlation of both a holistic qualitative and a quasi-quantitative approach to analyzing the interviews. The holistic qualitative approach identified student behaviors in four broad categories: problem-solving, conceptual understanding, test-taking strategies, and use of scientific language. The quasi-quantitative analysis allowed us to focus on more specific behavioral trends within these categories providing a more detailed picture of what middle-achieving students do when solving algorithmic and conceptual problems. Middle-achieving students demonstrated more variability when solving conceptual questions as compared to algorithmic questions, applying a mixture of behaviors that were characteristic of higher-achieving and lower-achieving students. Implications for teaching based on this research include the need to help middle-achieving students become aware of the difference between their approaches to solving algorithmic versus conceptual questions, emphasizing what they do correctly and how they can improve their problem solving.

The aim of this paper is to study the effect of the organic matter (OM) content on the effectiveness of the soil stabilization process using the biopolymer xanthan gum. Five different artificial soils with an organic matter content in the range of 1.5 to 7.7% are subjected to unconfined compressive strength (UCS) and oedometer tests to evaluate their strength, stiffness and compressibility, respectively. These results are complemented by an analysis of SEM images. The results from the UCS tests show a positive effect of the stabilization process in terms of strength and stiffness in a range of an OM content of 1.5 to 5.5%, but a detrimental effect for a higher OM content (7.7%) is observed. Due to the hydration of the biopolymer’s hydrogels, the compressibility tends to increase in specimens stabilized with xanthan gum. This effect is amplified for higher OM contents. The results of oedometer tests also show that the stabilization with xanthan gum brings about a significant decrease in the coefficient of consolidation, inducing an increase in the primary consolidation time.

The interest in applying gold nanoparticles is principally based on their monodispersity. Therefore, the synthesis of monodisperse gold nanocrystals has a key role in designing nanomaterials for their specific application. However, it can represent a cutting edge for many laboratories, mainly because it requires a variety of complex techniques for properly characterizing metal nanoparticles with expensive and rugged equipment. Herein, we propose an approach to synthesize nanoparticles with controlled monodispersity that can be adjusted through the factorial experimental plan 32 using response surface modeling (RSM). The particles were characterized on the basis of visual observation, UV–visible spectroscopy, transmission electron microscopy, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDS) and statistics. Our findings illustrate how factorial design is important to providing accurate projections of nanoparticle production using sodium citrate and borohydride as reducers. The proposed method leverages facile and inexpensive routes to synthesize monodisperse gold nanocrystals without the assistance of time-consuming image-based characterization tools. Keywords: Gold nanoparticles, Growth steps, Experimental design, Response surface methodology

Plasma samples obtained from stroke patients treated with recombinant tissue-type plasminogen activator (rt-PA) and not treated with rt-PA were evaluated with different HPLC methodologies to obtain information about the possible release of small molecules as a result of the thrombolytic treatment. Plasma samples, without derivatization and derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), were evaluated with a HPLC gradient method, which consisted of a mobile phase of 10 mM ammonium acetate buffered solution (pH = 5.3) and acetonitrile. Three different detection methods were applied: UV, fluorescence, and ESI-MS. The results obtained showed that a group of new highly hydrophilic compounds appeared in most samples analyzed from treated patients, just after the administration of rt-PA. These compounds appeared shortly after the administration of the drug and were detected during the first 24 h after treatment, disappearing from plasma after this time. These new compounds were not detected either in controls or in non-treated stroke patients, which suggests that they were released into the plasma as a consequence of the thrombolytic effect of the drug. Our results suggest that these new compounds might be free glycans. The use of AQC as a derivatizing reagent has demonstrated that the new compounds detected cannot contain primary or secondary amine groups in their structure. The molecular mass determined by ESI-MS (821 Da) suggests that if these compounds are free glycans they might be a high-mannose type.

Objectives: Orthognathic surgery is popular and provides patients with improved aesthetics and functionality. The procedure is considered safe and predictable. Possible complications do exist and can be life-threatening. The aim of this study is to assess the prevalence of intra- and post-operative complications, and to define possible correlations between diagnoses. Study Design: Medical records of 103 patients who underwent orthognathic surgery in a 4.5-year period (2013–2017), at the Baruch Padeh “Tzafon” Medical Center in Poriya (PMC), Israel, were retrospectively collected. The data were analyzed for descriptive statistics and non-parametrical tests. Results: In total, there were 56 complications in 45 patients (43.7% probability of complication per patient). Major complication occurred in only one case (1%), moderate complications appeared in 8.7% of the cases, while the most prevalent minor complications were temporary nerve injury and hardware issues (17.5% and 15.5%, respectively). Statistical analysis revealed that bi-max surgery has significantly higher (OR 1.34, CI 99% 1.05–1.69, p = 0.019) prevalence of complications than one-jaw surgery, as did skeletal class II patients (OR 2.75, CI 99% 2.25–3.35, p = 0.022), as compared to skeletal class III patients. Conclusions: Serious complications seem to be rare in orthognathic surgery at PMC.

In the activity described in this communication, the authors present a simple experiment that can be implemented with moderate operational costs and that allows students to acquire the manipulative skills necessary for chromatographic analysis of a familiar fuel that still plays a fundamental role in providing energy for transport of passengers and goods. The use of gasoline formulations in a laboratory activity presents students with a motivating subject of study and an opportunity to apply analytical procedures to the characterization of controlled substances used in vehicle fuel formulations.

Objective: To compare brain metabolism using [18F]fluorodeoxyglucose ([18F]FDG) positron emission tomography/computed tomography (PET/CT) in total thyroidectomy patients during hypothyroidism (levothyroxine withdrawal) or under recombinant human thyroid-stimulating hormone (rhTSH) against levothyroxine intake. Methods: A total of 12 patients were randomly divided into two groups. One group underwent the first [18F]FDG PET/CT brain scan after levothyroxine withdrawal (hypothyroidism condition) and repeated the scan 6 months later during regular levothyroxine intake (replacement condition). The other group underwent the first [18F]FDG PET/CT scan after receiving an rhTSH injection and maintained regular levothyroxine intake (rhTSH condition), and repeated the scan 7 months later during regular levothyroxine intake without rhTSH administration. The intra-group regional brain metabolisms were compared. Results: Under the hypothyroidism condition, brain metabolism was significantly reduced, namely in the bilateral pre-frontal, temporal, anterior cingulate, and primary motor cortices, insula, and striatum (uncorrected voxelwise p < 0.005); No significant differences were found between the rhTSH and replacement conditions. Conclusion: rhTSH administration could be a better option than levothyroxine withdrawal for 131I treatment, serum thyroglobulin measurement, or radioiodine scanning for patient follow-up.

Chagas disease is the most widespread contagious tropical disease in Latin America, being an important public health problem. Treatments against this disease are still very ineffective, presenting several side effects. Therefore, the search for alternative therapeutic solutions is urgent. In the present work, we evaluate the trypanocidal activity and the mechanism of action of a select series of synthetic 4-acyloxy-3-nitrocoumarins. All the coumarin derivatives showed moderate trypanocidal activity in trypomastigotes, along with low cytotoxicity. In addition, compound 1 decreased the number of infected Vero cells in an intracellular T. cruzi model. Electron spin resonance and electrochemical studies showed the formation of nitro radical anions. The Fukui index provided additional information to elucidate the proposed reduction mechanism. Furthermore, in vitro radical formation studies demonstrated the potential of these compounds to achieve higher concentrations of intracellular free radicals, proposing oxidative stress as a possible trypanocidal mechanism. Furthermore, no correlation was observed between the diffusion of these compounds, which shows that lipophilicity is not a predominant factor for activity. Elsevier Ltd. All rights reserved.

Bovine viral diarrhea virus (BVDV) belongs to the Pestivirus genus (Flaviviridae). In spite of the availability of vaccines, the virus is still causing substantial financial losses to the livestock industry. In this context, the use of antiviral agents could be an alternative strategy to control and reduce viral infections. The viral RNA-dependent RNA polymerase (RdRp) is essential for the replication of the viral genome and constitutes an attractive target for the identification of antiviral compounds. In a previous work, we have identified potential molecules that dock into an allosteric binding pocket of BVDV RdRp via a structure-based virtual screening approach. One of them, N-(2-morpholinoethyl)-2-phenylquinazolin-4-amine [1, 50% effective concentration (EC50) = 9.7 ± 0.5 μM], was selected to perform different chemical modifications. Among 24 derivatives synthesized, eight of them showed considerable antiviral activity. Molecular modeling of the most active compounds showed that they bind to a pocket located in the fingers and thumb domains in BVDV RdRp, which is different from that identified for other non-nucleoside inhibitors (NNIs) such as thiosemicarbazone (TSC). We selected compound 2-[4-(2-phenylquinazolin-4-yl)piperazin-1-yl]ethanol (1.9; EC50 = 1.7 ± 0.4 μM) for further analysis. Compound 1.9 was found to inhibit the in vitro replication of TSC-resistant BVDV variants, which carry the N264D mutation in the RdRp. In addition, 1.9 presented adequate solubility in different media and a high-stability profile in murine and bovine plasma.

In recent years, advanced nanohybrid materials processed as pharmaceuticals have proved to be very advantageous. Triptans, such as the commercially available intranasal sumatriptan (SMT), are drugs employed in the treatment of painful migraine symptoms. However, SMT effectiveness by the intranasal route is limited by its high hydrophilicity and poor mucoadhesion. Therefore, we designed hybrid nanoemulsions (NE) composed of copaiba oil as the organic component plus biopolymers (xanthan, pectin, alginate) solubilized in the continuous aqueous phase, aiming at the intranasal release of SMT (2% w/v). Firstly, drug-biopolymer complexes were optimized in order to decrease the hydrophilicity of SMT. The resultant complexes were further encapsulated in copaiba oil-based nanoparticles, forming NE formulations. Characterization by FTIR-ATR, DSC, and TEM techniques exposed details of the molecular arrangement of the hybrid systems. Long-term stability of the hybrid NE at 25°C was confirmed over a year, regarding size (~ 120 nm), polydispersity (~ 0.2), zeta potential (~ −25 mV), and nanoparticle concentration (~ 2.1014 particles/mL). SMT encapsulation efficiency in the formulations ranged between 41–69%, extending the in vitro release time of SMT from 5 h (free drug) to more than 24 h. The alginate-based NE was selected as the most desirable system and its in vivo nanotoxicity was evaluated in a zebrafish model. Hybrid NE treatment did not affect spontaneous movement or induce morphological changes in zebrafish larvae, and there was no evidence of mortality or cardiotoxicity after 48 h of treatment. With these results, we propose alginate-based nanoemulsions as a potential treatment for migraine pain.

Background: Dipeptidyl peptidase-4 (DPP-4) may be a suitable biomarker to identify people with severe asthma who have greater activation of the interleukin-13 (IL-13) pathway and may therefore benefit from IL-13-targeted treatments. We report the analytical performance of an Investigational Use Only immunoassay and provide data on the biological range of DPP-4 concentrations. Methods: We assessed assay performance, utilising analyses of precision, linearity and sensitivity; interference from common endogenous assay interferents, and from asthma and anti-diabetic medications, were also assessed. The assay was used to measure the range of serum DPP-4 concentrations in healthy volunteers and subjects with diabetes and severe, uncontrolled asthma. Results: The total precision of DPP-4 concentration measurement (determined using percentage coefficient of variation) was ≤5% over 20 days. Dilution analysis yielded linear results from 30 to 1305 ng/mL; the limit of quantitation was 19.2 ng/mL. No notable endogenous or drug interferences were observed at the expected therapeutic concentration. Median DPP-4 concentrations in healthy volunteers and subjects with asthma or Type 1 diabetes were assessed, with concentrations remaining similar in subjects with diabetes and asthma across different demographics. Conclusion: These analyses indicate that the ARCHITECT DPP-4 Immunoassay is a reliable and robust method for measuring serum DPP-4 concentration. Keywords: Asthma, Automated immunoassay, Biomarker, Dipeptidyl peptidase-4, IL-13

This study examined similarities and differences in study approaches reported by general chemistry students performing at different achievement levels. The study population consisted of freshmen enrolled in a required year-long general chemistry course at the U.S. Naval Academy. Students in the first and second semesters of the course were surveyed using a modified version of the published Approaches and Study Skills Inventory for Students (ASSIST) referred to as the M-ASSIST (Modified Approaches and Study Skills Inventory). Responses to items associated with using deep or surface approaches to studying were examined for students of three achievement levels (A/B, C, and D/F course grades) using both ANOVA and Structured Means Modeling to look for differences in study approaches between achievement levels. Results show that, with only 12 items, the M-ASSIST can be used to measure differences in reported use of deep and surface approaches by students in different achievement groups; that Structured Means Modeling can uncover significant differences that are not apparent with an ANOVA analysis of the same data; and that A/B and D/F students can be classified as reporting using either using primarily deep (A/B students) or primarily surface (D/F) study approaches. C students reported study approaches characteristic of both the A/B and D/F groups, leading to the interpretation that C students may be in an intermediate and possibly transitional state between the higher- and lower-grade groups. These results suggest a new understanding of C students as those who may not fully implement deep approaches to studying but, in general, demonstrate less reliance on surface approaches than lower-achieving students.

Students with an insufficient amount of time to study are becoming more prevalent in the general college population as many who enroll in college have competing responsibilities (full-time jobs, childcare, etc.). Such students are likely to choose study resources that they consider to be both effective and efficient. Students at the U.S. Naval Academy (USNA) are constrained in their study time because of their required course load and competing institutional daily requirements. The purpose of this study was to survey which resources students at USNA choose for studying and to look at the difference in choice in relation to different types of assessments and different student achievement levels in chemistry. Students (n = 1,015) were surveyed four times during the Fall 2013 semester after both instructor-written assessments and departmental multiple-choice common exams. In these surveys, students reported the main study resource they used to prepare for each assessment. A subset of students (n = 57) was interviewed soon after completing the third survey to better understand how the students used the resources they reported choosing. The results show a difference in study resources chosen depending on the type of assessment (instructor-written or common exam) and final course achievement level of the student. Application of these results to a broader audience of students who also have multiple time commitments may help chemical educators better format both the availability and content of chemistry study resources to help students of different achievement levels succeed in general chemistry.

This study aims to evaluate five different methodologies reported in the literature for developing fragility curves to assess the seismic performance of RC structures subjected to structural pounding. In this context, displacement-based and curvature-based fragility curves are developed. The use of probabilistic seismic demand models (PSDMs) on the fragility assessment of the pounding risk is further estimated. Linear and bilinear PSDMs are developed, while the validity of the assumptions commonly used to produce a PSDM is examined. Finally, the influence of the PSDMs’ assumptions on the derivation of fragilities for the structural pounding effect is identified. The examined pounding cases involve the interaction between adjacent RC structures that have equal story heights (floor-to-floor interaction). Results indicate that the fragility assessment of the RC structure that suffers the pounding effect is not affected by the examined methodologies when the performance level that controls the seismic behavior is exceeded at low levels of IM. Thus, the more vulnerable the structure is due to the pounding effect, the more likely that disparities among the fragility curves of the examined methods are eliminated. The use of a linear PSDM fails to properly describe the local inelastic demands of the structural RC member that suffers the impact effect. The PSDM’s assumptions are not always satisfied for the examined engineering demand parameters of this study, and thus may induce errors when fragility curves are developed. Nevertheless, errors induced due to the power law model and the homoscedasticity assumptions of the PSDM can be reduced by using the bilinear regression model.

The use of microalgal starch has been studied in biorefinery frameworks to produce bioethanol or bioplastics, however, these products are currently not economically viable. Using starch-rich biomass as an ingredient in food applications is a novel way to create more value while expanding the product portfolio of the microalgal industry. Optimization of starch production in the food-approved species Chlorella vulgaris was the main objective of this study. High-throughput screening of biomass composition in response to multiple stressors was performed with FTIR spectroscopy. Nitrogen starvation was identified as an important factor for starch accumulation. Moreover, further studies were performed to assess the role of light distribution, investigating the role of photon supply rates in flat panel photobioreactors. Starch-rich biomass with up to 30% starch was achieved in cultures with low inoculation density (0.1 g L−1) and high irradiation (1800 µmol m−2 s−1). A final large-scale experiment was performed in 25 L tubular reactors, achieving a maximum of 44% starch in the biomass after 12 h in nitrogen starved conditions.

Tributyltin (TBT) is an organotin chemical mainly used as biocide in marine antifouling paints. Despite the restrictions and prohibitions on its use, TBT is still an environmental problem due to its extensive application and subsequent release into the environment, being regarded as one of the most toxic chemicals released into the marine ecosystems. Microorganisms inhabiting impacted sites are crucial for their restoration since they have developed mechanisms to tolerate and break down pollutants. Nonetheless, transformation products resulting from the degradation process may still be toxic or, sometimes, even more toxic than the parent compound. The determination of the parent and degradation products by analytical methods, although necessary, may not be ecologically relevant since no information is provided regarding their ecotoxicity. In this study, marine bacteria collected from seven Portuguese fishing ports were isolated and grown in the presence of TBT. Bacteria that exhibited higher growth were used to bioremediate TBT-contaminated waters. The potential of these bacteria as bioremediation agents was evaluated through ecotoxicological assays using the sea snail Gibbula umbilicalis as model organism. Data suggested that some TBT-tolerant bacteria, such as Pseudomonas putida, can reduce the toxicity of TBT contaminated environments. This work contributes to the knowledge of TBT-degrading bacteria.

Root−associated microbial communities play important roles in the process of adaptation of plant hosts to environment stressors, and in this perspective, the microbiome of halophytes represents a valuable model for understanding the contribution of microorganisms to plant tolerance to salt. Although considered as the most promising halophyte candidate to crop cultivation, Salicornia ramosissima is one of the least-studied species in terms of microbiome composition and the effect of sediment properties on the diversity of plant-growth promoting bacteria associated with the roots. In this work, we aimed at isolating and characterizing halotolerant bacteria associated with the rhizosphere and root tissues of S. ramosissima, envisaging their application in saline agriculture. Endophytic and rhizosphere bacteria were isolated from wild and crop cultivated plants, growing in different estuarine conditions. Isolates were identified based on 16S rRNA sequences and screened for plant-growth promotion traits. The subsets of isolates from different sampling sites were very different in terms of composition but consistent in terms of the plant-growth promoting traits represented. Bacillus was the most represented genus and expressed the wider range of extracellular enzymatic activities. Halotolerant strains of Salinicola, Pseudomonas, Oceanobacillus, Halomonas, Providencia, Bacillus, Psychrobacter and Brevibacterium also exhibited several plant-growth promotion traits (e.g., 3-indole acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, siderophores, phosphate solubilization). Considering the taxonomic diversity and the plant-growth promotion potential of the isolates, the collection represents a valuable resource that can be used to optimize the crop cultivation of Salicornia under different environmental conditions and for the attenuation of salt stress in non-halophytes, considering the global threat of arable soil salinization.

Recent advances in the production and development of two-dimensional transition metal dichalcogenides (2D TMDs) allow applications of these materials, with a structure similar to that of graphene, in a series of devices as promising technologies for optoelectronic applications. In this work, molybdenum disulfide (MoS2) nanostructures were grown directly on paper substrates through a microwave-assisted hydrothermal synthesis. The synthesized samples were subjected to morphological, structural, and optical analysis, using techniques such as scanning electron microscopy (SEM), X-ray diffraction (XRD), and Raman. The variation of synthesis parameters, as temperature and synthesis time, allowed the manipulation of these nanostructures during the growth process, with alteration of the metallic (1T) and semiconductor (2H) phases. By using this synthesis method, two-dimensional MoS2 nanostructures were directly grown on paper substrates. The MoS2 nanostructures were used as the active layer, to produce low-cost near-infrared photodetectors. The set of results indicates that the interdigital MoS2 photodetector with the best characteristics (responsivity of 290 mA/W, detectivity of 1.8 × 109 Jones and external quantum efficiency of 37%) was obtained using photoactive MoS2 nanosheets synthesized at 200 °C for 120 min.

To understand the physicochemical properties and catalytic activity during the pyrolysis of atmospheric petroleum residue, a template-free ZSM-5 zeolite was synthesized using a direct method without additional seeds or an organic structure director and compared with conventionally synthesized ZSM-5. The crystallinities of the two zeolites were evaluated by XRD and FTIR and were quite similar; however, structural analyses using SEM and argon physisorption revealed that the zeolites diverged in particle diameter and in the external surface area of the micropores. The synthesis procedure without a template incorporated additional aluminum into the crystalline network, according to ICP-AES and TPD NH3 experiments. The catalytic pyrolysis performed over the template-free ZSM-5 generated results comparable to those for pyrolysis performed over the conventional ZSM-5 according to its hydrocarbon distribution. The selectivity to aromatics compounds was exactly the same for both ZSM-5 zeolites, and these values stand out compared to thermal pyrolysis. The template-free ZSM-5 produced 20% of light hydrocarbons (C4-C6), where such compounds are olefins and paraffins of great interest to the petrochemical industry. Therefore, template-free ZSM-5 is promising for industrial use due to its lowered synthesis time, low-cost and significant distribution to light hydrocarbons.

The aim of this work was to review the reported information about the phospholipid composition of lecithins derived from several natural sources (lipids of plant, animal, and marine origin) and describe their main applications for the cosmetic, food, and pharmaceutical sectors. This study was carried out using specialized search engines and according to the following inclusion criteria: (i) documents published between 2005 and 2020, (ii) sources of lecithins, (iii) phospholipidic composition of lecithins, and (iv) uses and applications of lecithins. Nevertheless, this work is presented as a narrative review. Results of the review indicated that the most studied source of lecithin is soybean, followed by sunflower and egg yolk. Contrarily, only a few numbers of reports focused on lecithins derived from marine animals despite the relevance of this source in association with an even higher composition of phospholipids than in case of those derived from plant sources. Finally, the main applications of lecithins were found to be related to their nutritional aspects and ability as emulsion stabilizers and lipid component of liposomes.

Due to its properties, paper represents an alternative to perform point-of-care tests for colorimetric determination of glucose levels, providing simple, rapid, and inexpensive means of diagnosis. In this work, we report the development of a novel, rapid, disposable, inexpensive, enzyme-free, and colorimetric paper-based assay for glucose level determination. This sensing strategy is based on the synthesis of gold nanoparticles (AuNPs) by reduction of a gold salt precursor, in which glucose acts simultaneously as reducing and capping agent. This leads to a direct measurement of glucose without any enzymes or depending on the detection of intermediate products as in conventional enzymatic colorimetric methods. Firstly, we modelled the synthesis reaction of AuNPs to determine the optical, morphological, and kinetic properties and their manipulation for glucose sensing, by determining the influence of each of the reaction precursors towards the produced AuNPs, providing a guide for the manipulation of nucleation and growth. The adaptation of this synthesis into the developed paper platform was tested and calibrated using different standard solutions with physiological concentrations of glucose. The response of the colorimetric signals obtained with this paper-based platform showed a linear behavior until 20 mM, required for glycemic control in diabetes, using the Red × Value/Grey feature combination as a calibration metric, to describe the variations in color intensity and hue in the spot test zone. The colorimetric sensor revealed a detection limit of 0.65 mM, depending on calibration metric and sensitivity of 0.013 AU/mM for a linear sensitivity range from 1.25 to 20 mM, with high specificity for the determination of glucose in complex standards with other common reducing interferents and human serum.

Transparent oxyfluoride glass–ceramics containing NaGdF4 nanocrystals were prepared by melt-quenching and doped with Er3+ (0.5 mol%) and different amounts of Yb3+ (0–2 mol%). The selected dopant concentration the crystallization thermal treatments were chosen to obtain the most efficient visible up-conversion emissions, together with near infrared emissions. The crystal size increased with dopant content and treatment time. NaGdF4 NCs with a size ranging 9–30 nm were obtained after heat treatments at Tg + 20–80 °C as confirmed by X-ray diffraction and high-resolution transmission electron microscopy. Energy dispersive X-ray analysis shows the incorporation of rare earth ions into the NaGdF4 nanocrystals. Near-infrared emission spectra, together with the up-conversion emissions were measured. The optical characterization of the glass–ceramics clearly shows that Er3+ and Yb3+ ions are incorporated in the crystalline phase. Moreover, visible up-conversion emissions could be tuned by controlling the nanocrystals size through appropriated heat treatment, making possible a correlation between structural and optical properties.

The anodic oxidation of oxytetracycline was performed with success using as anode a boron-doped diamond electrode. The experiments were conducted in batch mode, using two different electrochemical cells: an up-flow cell, with recirculation, that was used to evaluate the influence of recirculation flow rate; and a stirred cell, used to determine the influence of the applied current density. Besides oxytetracyclin electrodegradation rate and mineralization extent, oxidation by-products were also assessed. Both the flow rate and the applied current density have shown positive influence on the oxytetracycline oxidation rate. On the other hand, the mineralization degree presented the highest values at the lowest flow rate and the lowest current density tested. The main oxidation by-products detected were oxalic, oxamic and maleic acids.

Soil invertebrates have been widely used in ecotoxicology studies for decades, although their use as in vitro models, albeit promising, has not been pursued as much. The immune cells of earthworms (coelomocytes) and the coelomic fluid can be used, and are a highly relevant in vitro system. Although it has been tested before, to cover the testing of nanomaterials (NMs), several challenges should be considered. NMs characteristics (dispersibility, agglomeration, etc.) can interfere with the common in vitro methodologies, not only during exposure, but also during the measurements. Here, we have assessed the effect of a CuO NMs case study using surface-modified particles, functionalized for safe-by-design strategies with ascorbate, citrate, polyethylenimine, and polyvinylpyrrolidinone, plus the pristine CuO NMs and copper chloride (CuCl2) for comparison. Eisenia fetida’s coelomocytes were exposed for 24 h via the coelomic fluid. Changes in cell viability were evaluated using flow cytometry. All materials affected the cells in a dose-related manner, where CuCl2 was the most toxic followed by the citrate-coated CuO NM. There was a strong correlation between NM characteristics, e.g., the hydrodynamic size, and the EC50 (50% Effect Concentrations) values. This screening further confirms the potential for the usage of the standard earthworm model as an in vitro standard. Further detailed in vitro studies are needed using other NMs aiming toward their implementation and standardization. Additional cell endpoints can also be assessed, making it a high content tool for mechanistic understanding.

The electrochemical treatment of leachate samples from a Portuguese intermunicipal sanitary landfill was carried out using anodic oxidation. The treatment was performed in a pilot plant that possesses an electrochemical cell, with boron-doped diamond electrodes, working in batch mode with recirculation. The influence of the applied current density and the flow rate on the performance of the electrochemical oxidation was investigated. Current density was decreased by steps, during the degradation, in order to study this effect on the efficiency of the process. For the assays run at equal flow rate and initial current intensity, chemical oxygen demand (COD) removal seems to depend mainly on the charge passed and the variation of the current density during the anodic oxidation process can reduce the energetic costs. An increase in the recirculation flow rate leads to an increase in the organic load removal rate and a consequent decrease in the energetic costs, but it decreases the nitrogen removal rate. Also, the bias between dissolved organic carbon and COD removals increases with flow rate, indicating that an increase in recirculation flow rate decreases the mineralization index.

This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.

A new, integrated laboratory curriculum was recently developed at the U.S. Naval Academy in response to the 1999 ACS Committee on Professional Training guidelines that required inclusion of biochemistry and a stronger emphasis on student research. To meet these ACS requirements and to introduce more student choice in the major, we embarked on a complete redesign of the laboratory program, reducing eleven credit hours of traditional laboratory courses into a cohesive, eight-credit, four-semester sequence of integrated laboratory courses covering the core areas of chemistry. These courses are designed along broader themes with many experiments simultaneously exploring concepts from two or more subdisciplines of chemistry. Although the integrated laboratory concept has been applied at other institutions, this curriculum is unique in its design and scope. The program begins in the sophomore year and is completed in the junior year, allowing students to pursue research and advanced courses in their senior year. The integrated laboratory concept offers many attractive benefits, although there are potential difficulties: several of these are discussed as well. This paper may assist faculty contemplating a similarly comprehensive curricular change to carefully weigh the factors necessary to determine the feasibility and sustainability of such a program in their own unique educational environment. (Contains 3 tables and 1 list.)

An experiment related to the measurement of fluorescence lifetime for an undergraduate instrumental analysis or physical chemistry laboratory that highlights relative rates of electronic transitions in molecules and introduces students to data collection of a pulsed signal is illustrated. The experiment of the long fluorescence lifetime of Ru(bpy)[subscript 3][superscript 2+] around 600 ns was performed with aqueous solutions using light-emitting diode as an excitation source which allowed data to be collected on a modest oscilloscope.