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Flow cytometric sorting is a vital tool in biological research and clinical diagnostics. Theoretically, a high‐speed jet‐in‐air sorter is a fluorescent‐activated cell sorting sorter that ideally processes cells with high purity, yield, and viability. However, high‐speed jet‐in‐air sorting is a complex process due to its inherent requirements for high fluidic stability and electronic and timing precision. Here, we report that an additional manual correction of drop delay leads to improved cell yield. Adding 2% FBS to the loading buffer had no significant effect on the fate of sorted cells in 4 h. However, the addition of a suitable concentration of FBS/BSA in the collecting buffer resulted in a notable increase in cell count and proliferation and a significant decrease in cell apoptosis for cell lines and primary cells. Moreover, the level of gene expression remained steady in the 5% FBS collecting buffer. In summary, here we demonstrate techniques that can be easily followed to refine sorted yields of healthy cells., Flow cytometric sorting is a vital method in biological research and clinical diagnostics. Obtaining high‐quality sorted cells is an ongoing problem. Here, we demonstrate that a certain concentration of FBS helps to increase cell yield and cell viability and keep gene expression stable. Our work provides an effective sorting strategy.

Kinsenoside is the major bioactive component from herbal medicine with a broad range of pharmacological functions. Goodyeroside A, an epimer of kinsenoside, remains less explored. In this report we chemically synthesized kinsenoside, goodyeroside A and their analogues with glycan variation, chirality inversion at chiral center(s), and bioisosteric replacement of lactone with lactam. Among these compounds, goodyeroside A and its mannosyl counterpart demonstrated superior anti-inflammatory efficacy. Furthermore, goodyeroside A was found to suppresses inflammatory through inhibiting NF-κB signal pathway, effectively. Structure-activity relationship is also explored for further development of more promising kinsenoside analogues as drug candidates.

Acidic compounds were enriched from a water decoction of Portulaca oleracea using 717 anion exchange resin column chromatography. A total of 22 compounds including 9 catecholamine derivatives, of which six were rare sulfonic acid derivatives, and 9 nitro derivatives, were further isolated through various column chromatographic methods, and their structures were elucidated by interpreting their spectroscopic data and ECD calculations. Among them, 16 compounds were isolated from P. oleracea for the first time, 8 of which were undescribed compounds and four compounds were natural products. Pharmacological screening indicated that cis-3-(3-nitro4-hydroxyphenyl)-methyl acrylate exhibited anti-inflammatory activity, measured as inhibition of nitric oxide production in LPS-stimulated RAW264.7 macrophage cells, with an EC50 value of 18.0 mu M, The compounds showed only weak anti-microbial activity with (2R)-(+)-2-chlom 3 (3-nitro-4-hydroxyphenyl)-propionic acid methyl ester inhibiting Candida albicans with a MIC of 256 mu g/mL, and 3-methoxy-4,5-dinitrophenol inhibiting Shigella sonnei with a MIC of 512 mu g/mL. Refereed/Peer-reviewed

The effect of doping IB metals (Cu, Ag, and Au) on the photocatalytic activity of monoclinic Bismuth vanadate (BiVO4) was investigated by predicting their electronic and optical properties based on the first-principle calculation. For the Cu-doped system, the formation of shallow acceptor level will narrow the band gap and trap the electrons which may enhance the separation of photo-induced carriers. And the doping of Ag has little effect on the electronic structure. However, the doping of Au will introduce a deep localized impurity level which should serve as both charge traps and recombination centers, and hinder the separation of photo-induced carriers. With incorporation of Cu, the mobility of charge carriers is enhanced due to the smaller effective mass, but the Ag and Au doping would not improve the transfer of charges. In summary, only the doping of Cu has positive effect on improving the photocatalytic activity of monoclinic BiVO4. © 2015 Wiley Periodicals, Inc.