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This is a protocol for the enrichment of phenazine-1-carboxylic acid oxidizing microbes from a soil sample.

This protocol describes the culturing conditions used to grow S. robusta in the lab for all experiments.

This protocol describes how to deposit several cells of S. robusta in droplets on a petri dish, allowing the experimenter to track the growth of approximately single cell isolates with high throughput. This was used to determine drug sensitivity. We found that in, liquid culture, S. robusta is sensitive to 10 ug/mL puromycin and 250 ug/mL glyphosate. One caveat to keep in mind is that S. robusta appears more tolerant to antibiotics when grown on agar plates, being insensitive to up to 20 ug/mL of puromycin, for example. We recommend selection in liquid culture.