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Preservation of cells during crystallization of the cell suspension is influenced by two types of damaging factors. The first type of cryoinjury occurs during the crystallization of the extracellular environment and is caused by dehydration of cells, increasing the concentration and ionic strength of extracellular and intracellular solutions. As the cooling rate rises, the damage rate of the first type decreases as a result of the reduced time of action of damaging factors. The second type of cryoinjury is intracellular crystallization, the probability of which enhances at high cooling rates, is considered the most destructive to cells. The optimal linear cooling rate for PK-15 cells is determined using a physico-mathematical model, which describes the probability of cryoinjury of cells in the linear freezing mode and is based on the two-factor theory of cryoinjury, thermodynamic theory of homogeneous crystallization and general theory of activation-type processes. The findings have shown that within the range of cooling rates < 0.5 °C/min the cryoinjury of PK-15 cells occurs mainly due to the effects of the solution, and at cooling rates > 2.5 °C/min this was mainly resulted from an intracellular crystallization. The dependence of the percentage of damaged cells on the cooling rate has a relatively wide minimum within the range of cooling rates of 0.5 °C/min… 2.5 °C/min.

Reorientation of hydrides in the course of dry storage of spent nuclear fuel is a possible mechanism for the degradation of the properties of fuel claddings made of alloy E110 (Zr–1% Nb) based on spongy zirconium in VVER reactors. To determine the degree of reorientation of hydride precipitates in fuel claddings under conditions that simulate the conditions of dry storage, a series of tests have been performed for unirradiated samples with a hydrogen content of 100 and 200 wppm at various levels of circumferential stresses up to 100 MPa for 40 days. The orientation of hydride precipitates is determined using metallographic measurements. To assess the mechanical characteristics, the ring specimens have been tested for static uniaxial tension. The hydrides are analyzed using transmission electron microscopy and X-ray diffraction analysis. It is shown that test conditions lead to partial reorientation of hydride precipitates, which contributes, in turn, to a decrease in the plasticity.

The culture media quality, which depends to a large extent on growth promotion properties, determines the reliability and accuracy of test results obtained by microbiological methods. The procedure for culture media preparation is quite labourconsuming and includes several stages: successive dissolution of components exactly as specified in the recipe (qualitative and quantitative composition), sterilisation, adjusting the pH of the medium, and testing of growth promotion properties—therefore it is important to demonstrate the stability of each particular culture medium.The aim of the study was to evaluate growth promotion properties of the culture media prepared in the laboratory from a dry mixture, and to assess their stability during long-term storage.Materials and methods: stability testing was performed for fluid thioglycollate medium (FTM) and soybean-casein digest broth (SCD) prepared in the laboratory. FTM growth promotion properties were tested using the following test microorganisms: Bacillus subtilis ATCC 6633, Clostridium sporogenes ATCC 19404, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538. SCD growth promotion properties were tested using: Aspergillus brasiliensis ATCC 16404, Candida albicans ATCC 10231, Bacillus subtilis ATCC 6633. The stability study was carried out for 6 months, assessing changes in appearance and growth promotion properties. The test media were stored at room temperature and in a refrigerator at 2–8 °C.Results: no growth of the anaerobic microorganism Clostridium sporogenes was observed in FTM after 3 months, regardless of storage conditions. Later on, there was no growth of the gram-positive microorganisms Bacillus subtilis and Staphylococcus aureus, which are more sensitive to the storage conditions than Pseudomonas aeruginosa. The growth promotion properties of the SCD prepared in the laboratory did not change during 6 months of storage.Conclusions: FTM prepared from a dry mixture remains stable and retains its growth promotion properties for no more than two months when stored in a refrigerator at 2–8 °C. SCD can remain stable for 6 months, both at room temperature and when stored in the refrigerator.

A terpyridine derivative and an iridium complex catalyze the C–H borylation of a stoichiometric amount of a fluoroarene with high ortho-selectivity and tolerance of functional groups such as bromid...

—An increase in the level of ROS during the prenatal period, which is associated with active differentiation of cells, leads to the formation of dose-dependent teratogenic effects. The aim of this study was to determine the relationship between the duration of stress experienced by pregnant female rats in the third trimester with the degree of shift of free radical homeostasis in blood plasma and at different levels of the central nervous system. We analyzed changes in the level of activity of superoxide dismutase, changes in the products of lipid peroxidation and changes in oxidative modification of proteins in homogenates of the cerebral cortex, hypothalamus, cerebellum, and spinal cord of prenatally stressed sexually mature male outbred white rats. To this aim, their dams from 16 to 19 days of pregnancy were exposed daily to immobilization stress in plastic cases for one or three hours. Females of the control groups were kept in standard vivarium conditions with free access to food and water. Prenatal stress influenced the free radical homeostasis of all the CNS regions studied in 90-day-old male rats. More significant effects were observed after 3 hours of immobilization. The offspring of stressed mothers were characterized by more pronounced shifts in free radical balance. As a result of one-hour of daily immobilization from 16 to 19 days of pregnancy, we observed an increase in the indices of oxidative modification of the protein and lipid components in the mature offspring of stressed mothers, while the consequences of three-hour-long prenatal immobilization, on the contrary, are characterized by a decrease in the studied indices of free radical oxidation products and an increase in SOD activity. Regardless of the duration of exposure, the most pronounced shifts in the studied parameters of free radical homeostasis were in the hypothalamic region.

The values of permeability coefficients to water molecules and cryoprotectants are demanded to select the optimal duration of exposure of cells in cryoprotective media at the stage of their preparation for cryopreservation, as well as to find optimal cooling and warming rates during the freeze-thawing of cell suspensions. The necessary numerical values of such cell parameters as the osmotically inactive volume α and the surface-area-to-volume ratio γ were obtained for the analytical evaluation of the permeability coefficients of the PK-15 cells’ plasma membranes using physico-mathematical modelling. The permeability coefficients kp of the plasma membranes of PK-15 cells to 1,2-PD, EG, DMSO and glycerol cryoprotectants molecules, as well as the filtration coefficients Lp to water molecules at temperatures of 25, 15 and 5°C were determined by approximating the experimental data of the change in relative volume of cells on exposure time in the studied solutions by theoretical curves calculated on the basis of physical and mathematical model of passive transport of water and permeable substances under the condition of their maximum coincidence. The value of the activation energy of the transmembrane transfer of molecules of these substances is calculated

The electrogenic properties of plant–microbial fuel cells (P-MFCs) assembled into a battery are studied. The operation of a single cell is studied experimentally in comparison with parallel and series connections of cells, which are two options for connection in an electrical circuit. A potential difference of ~70 mV, which gradually vanishes, is registered in a P-MFC that does not include a plant organism. We hypothesize that the root system, with involvement of electrogenic chemoorganotrophic microorganisms, gives rise to diffusional electromotive force. We show that in series connection of three fuel cells the bioelectric potential generated by an individual cell, which is 170 mV, increases only by a factor of 1.5. In parallel connection of three cells, the current produced under a load increases, but also only by a factor of 1.5, and this takes place at later developmental stages of the plants, which is presumably caused by triggering certain compensatory mechanisms that diminish the electrogenic properties of the plants.

The research involved female mongrel white rats (n = 16) and their offspring (sexually mature males, n = 75). Pregnant females were divided into 4 groups: control (intact animals), stress (from the 16th to the 19th day of pregnancy females were subjected to 3-hour immobilization in plastic cases), stress+α-tocopherol (against the background of immobilization, females received α-tocopherol at a dose of 1 mg per 100 g of weight starting from the 2nd day of pregnancy), and α-tocopherol (females received α-tocopherol in the same dose and regimen as the group above). The control and stress groups received vegetable oil instead of α-tocopherol. In 3-month-old female offspring, we studied behaviour and determined the levels of products of free radical modification of proteins and lipids, total antioxidant activity as well as superoxide dismutase and ceruloplasmin activity in the blood plasma. Prenatal stress increased the exploratory and motor activity in offspring and modified the level of free radical homeostasis in the serum of male rats: there was an increase in the level of products of oxidative modification of proteins, thiobarbituric acid reactive substances concentration, end products of lipid peroxidation (Schiff bases), total antioxidant activity and ceruloplasmin activity, as well as a decrease in superoxide dismutase activity. Alpha-tocopherol taken by females during pregnancy brought all the oxidation parameters modified by immobilization to the values of the control group; the levels of primary products of oxidative modification of proteins and lipid peroxidation decreased two-fold, compared with the stress group. The activity of superoxide dismutase and ceruloplasmin returned to the control values, but the total antioxidant activity remained at the level of the stress group. Thus, there is reason to assume that α-tocopherol has a significant corrective effect on the intensity of free radical processes in prenatally stressed white male rats.

We studied the photophysical processes of halogenated fluorescein derivatives (eosin (E), erythrosine (ER), and Rose Bengal (RB)) in aqueous micellar solutions using dynamic light scattering and stationary and time-resolved fluorescence spectroscopy. The introduction of dye molecules into reverse AOT micelles causes an increase in their hydrodynamic radii Rh. The kinetics of fluorescence of the studied dye molecules in reverse micelles was investigated. The average time $$\langle \tau \rangle $$ of the excited state decreases with increasing Rh for eosin, erythrosine, and Rose Bengal, which is associated with an increase in the mobility of water molecules and a weakening of the effect of geometric limitation of dye molecules. The degrees of fluorescence anisotropy r of dye molecules in reverse micelles were measured; the r values in micellar systems are greater than in aqueous solutions and decrease with increasing Rh. For the studied dye molecules in micellar systems, the rotational correlation time θ was determined, which decreases for all studied dyes with increasing Rh, which indicates a decrease in the microviscosity of a limited aqueous medium inside the micelle. It is found that θE > θER > θRB; that is, the effect of the internal heavy atom appears in the value of the rotational correlation time.

In experiments in mice, the radioprotective efficacy of sugar-containing compounds was studied based on the condensation products of thioglycolic (TGH) and thiosalicylic (TSH) hydrazides with a series of natural aldoses: the monosaccharides D-glucose (Glс) and D-galactose (Gal) and the disaccharides D‑lactose (Lac) and D-maltose (Mal). It was shown that the use of Glc-TGH and Mal-TSH preparations increases the survival rate of deadly irradiated animals by 25% when administered 1 h before irradiation and by 40% when administered 24 h before irradiation. It is assumed that the leading role in the radioprotective activity of the drugs is played by the protection of the hematopoietic stem cells of the test animals.

TEM, SEM, and APT techniques have been used to analyze radiation-induced components of metal structure of fragments cut from the pressure vessel internals of Novovoronezh NPP Unit No 3 after 45 years of operation. The fragments differed in the neutron damaging doses (from 14 to 43 dpa) and the irradiation temperature (from 285 to 315°C). The density and dimensions of titanium carbides and carbonitrides, dislocation loops, radiation-induced voids, segregations, and nanoscale precipitates were determined. The contributions of structural components to the radiation hardening of the investigated fragments of 18Cr-10Ni-Ti stainless steel were estimated.

The results of microstructural studies of the fragments of E110 alloy fuel rod cladding specimens based on sponge and electrolytic zirconium after the operation as a part of VVER-1000 fuel assemblies with the subsequent creep tests under axial loading are presented. It was shown that, during the creep tests, the studied specimens showed no changes in the chemical composition, average size, and bulk density of the second phases, including radiation-induced ones. It was found that, during the creep tests, dislocation loops were annealed, i.e., an increase occurred in their average size with a simultaneous decrease in bulk density. It was shown that the specimens of fuel rod claddings made of an alloy based on electrolytic zirconium demonstrated more significant creep resistance compared to the sponge-based zirconium alloy specimens, which is related to a higher density of globular β-Nb precipitates in the irradiated electrolytic zirconium specimens.

The luminescence spectral characteristics of eosin, erythrosine, and rose Bengal molecules in reverse micelles of sodium bis(2-ethylhexyl)sulfosuccinate were investigated. Inclusion of the dye molecules in the reverse micelles leads to an increase of their hydrodynamic radii Rh, and a linear relationship is observed between this increase ΔRh and the degree of hydration w. The increase of Rh leads to shift in the absorption and fluorescence spectra and increases the Stokes shift. The ratio of the dipole moments of the molecules in the excited and ground states μe/μg increases with increase of Rh.

We investigate the effects that the spectral composition of radiation that plants are exposed to during their development has on the fractionation of carbon isotopes between atmospheric carbon dioxide and the primary products of photosynthesis in plant tissues. The difference in carbon isotope composition between carbon in air near the leaves and carbon in the leaves varies in the range of 7 to 19 depending on the spectral composition of incident light, with an increase in the red component resulting in enrichment of the leaves with the light isotope 12C. This differences are (i) the measure of isotope fractionation during the vital activity of plants and (ii) the rate of carbon assimilation due to photosynthetic reactions.

The formation of complexes with nanomarkers of the fluorescein family and BSA in aqueous solutions is studied and its constants are determined. Three different models are plotted to interpret the experimental data. The effect of saturating a complex of nanomarkers and BSA, the presence of negative cooperativity in complexation, and the dependence of complexation on the mutual combination of the values of electrostatic charges on the protein and nanomarkers are found and explained using these models. The parameter of nanomarker affinity toward BSA during complexation is calculated.

Geological, hydrological, hydrochemical, and biological research has been carried out in the eastern tropical Atlantic. Based on the multibeam echo-sounding and seismic profiling north of the Kane Gap (Sierra Leone Rise), an extensive sedimentary body (contourite fan) has been identified. Data of hydrophysical sounding allows assuming the existence of an overflow of the Antarctic Bottom Water (AABW) through the Kane Gap and its northward penetration. In the sediment cores (Middle Pleistocene to Holocene age), an influence of the AABW is reflected in the alternation of the high and medium carbonate dissolution rates.

A study of allergenic properties of the developed dermatological film with liquid aloe extract and actovegin was conducted. The experiments were carried out by the method of cutaneous samples and by the method of epicutaneous sensitization in guinea pigs. The results were evaluated using the scale of S.V. Suvorov. As a result of the work done, allergenic properties have not been identified.

The fluorescence characteristics of eosin in solutions of bovine serum albumin (BSA) were studied at various pH. The quenching of eosin fluorescence with increasing concentration was found to result from two competing processes: binding of BSA molecules with eosin molecules and the formation of associated species of the dye. The extent of association as well as the structural and thermodynamic parameters of eosin were determined in buffer and BSA solutions at different pH. Variation of the buffer solution pH led to change of these characteristics. The effect of the formation of associated species of eosin in BSA solutions on the dynamic quenching constant of eosin fluorescence was examined. The formation of associated species of eosin was shown to lead to a decrease in the dynamic quenching constant of this dye, which is proportional to the pH of the solution.

The paper presents microstructural studies of specimens cut from fuel elements made of E110 spongy zirconium-based alloy after operation in a VVER-1000 before reaching the burnup of ~35 MWd/kg U. As a result of exposure to high temperatures and neutron irradiation, significant changes in the phase composition of fuel cladding materials appear: change in the size, density, and composition of β-Nb particles; change in the composition of the Laves phase; formation of dislocation loops of α type, as well as δ and γ hydrides. The main structural elements determining the degradation of the mechanical properties of the E110 alloy under irradiation are dislocation loops and fine-phase precipitates owing to their relatively large density. The data obtained can be used to construct dose dependences of microstructural changes with the aim of predicting the residual life of claddings and fuel assemblies as a whole.

The radiation-induced structural elements in the materials of water-moderated water-cooled reactor pressure vessels have been studied by TEM and atomic probe tomography at the National Research Center Kurchatov Institute. The effect of Ni concentration in the range from 0.34 to 1.28 wt % on the formation of phases under fast neutron irradiation has been analyzed. The volume density of radiation-induced phases has been shown to depend on the Ni concentration in steel; the phase sizes and compositions remain almost the same. The volume density of these precipitations affects the radiation hardening, one of the radiation embrittlement mechanisms. The quantitative parameters of radiation-induced phases have been shown to control the service life growth, the enhancement of the thermal stability, and the melting technology of reactor vessel steels bearing nickel in the 0.3–0.7 wt % range.

Aluminum-, iron- and titanium-containing metal-organic frameworks based on terephthalic acid was prepared and was characterized using BET, XRD, TGA and FT-IR techniques. Sorption of food dyes Indigo carmine, Tartrazine and Ponceau 4R with synthesized metal-organic frameworks was investigated in wide range of pH. It was established that the sorption of all dyes is better in acid media. It is found that the used metal-organic frameworks can be easily recycled at least five times, via ethanol washing. Metal-organic frameworks can be used as effective and easy regenerable sorbents for wastewater treatment from organic dyes of food industry.

The results of the reconstruction of Wolf numbers from the 11th century until the middle of the 19th century A.D. based on radiocarbon data are presented. This time span includes the Oort, Wolf, Sporer, Maunder, and Dalton minima of solar activity, as well as the Medieval Solar Maximum (the 12th century and the first half of the 13th century A.D.) and the Late Medieval Maximum (the second half of the 14th century). It is known that the climate changed appreciably over the studied period: there were changes in the global temperature and in the concentration of carbon dioxide in the Earth’s atmosphere; more precisely, there was the Little Ice Age. This work differs from previous reconstructions of other authors in taking into account the influence of climate changes on the radiocarbon content in examined samples at that time span. During the reconstruction, changes in carbon dioxide content and changes in the regimen of carbon dioxide exchange between the ocean and the atmosphere are considered. It is especially important to consider changes in these parameters for the Little Ice Age, which was accompanied by significant changes in global temperature and the carbon dioxide content in the Earth’s atmosphere.

The dimerization processes and its thermodynamic parameters of fluoronic dyes (fluorescein (F), eosin (E), erythrosine (ER), bengal rose (BR)) in reverse micelles of AOT with different hydrodynamic radius R h are studied. The dimerization constants and its efficiency (the degree of dimerization of dye molecules (1-X)) were determined from the experimental data. It is found that an increase in the intercombination conversion due to the heavy atom effect leads to an increase of the value of (1-X). At the same time, the heavy atom effect affects the dye dimerization process for all the studied values of R h . The linear dependence of (1-X) on R h is observed. The slope of this dependence is affected by both the mass of the internal heavy atom and the charge of the anionic forms of dyes. It was found that there is a different structure of dye dimers for different R h for all the studied systems - different angles α between the molecules in the dimer. A linear dependence of α on R h is observed. At the same time, the growth gradients α ( R h ) practically do not differ for F, E, and BR and they are of the greatest importance for the studied water-micellar solutions of dyes. The growth of α from R h is insignificant for ER. The thermodynamic parameters (such as Gibbs potential ΔG, enthalpy (ΔH and entropy ΔS) were calculated from the experimentally measured dependences of the dimerization constant on the temperature. ΔH R h , that indicates that the dimerization reaction in the studied systems is controlled by enthalpy. The obtained linear relationship between TΔS and ΔH indicates the existence of enthalpy–entropy compensation in the dimeric reactions of the molecules of the studied dyes. The linear correlation observed between the values of TΔS and ΔH allowed us to establish that the higher the molecular weight of the halogen in the dye molecule, the more effectively an increase in ΔH contributes to the dimeric stability.

The experimental plant-microbial fuel cell based on the gradient of bioelectric potentials created in the rhizosphere and compatible with the production of plant products was created and tested.

The article is devoted to the problem of phosphoric nutrition of rice on chestnut soils of the northernmost zone of rice cultivation in Russia. The paper reveals the role of water-soluble fractions of phosphorus, aluminosilicates, iron phosphates and calcium phosphates in the nutrition of rice plants during the growing season. The influence of the previous culture on the content and consumption of these fractions was established.

The adsorption of serine anion on a smooth and platinized platinum electrode is investigated. The stationary and kinetic adsorption isotherms are plotted. For both smooth platinum electrodes and Pt(Pt) electrodes, the kinetics of the processes under investigation obeys the Roginsky–Zeldovich equation, and the stationary coverage is described by the Temkin isotherm. The main thermodynamic characteristics (adsorption equilibrium constant and change in the Gibbs free energy) of the process of adsorption of serine anion on both electrodes are found.

The effect of sodium alginate on the viability and kinetics of phase transformations of Saccharomyces cerevisiae cells during cryopreservation was investigated. There were determined the optimal conditions for yeast cell cryopreservation such as: the cooling rate of 1 deg/min in alginate-containing cryoprotective media (cell viability of (90.79 ± 2.01)% and (88.11 ± 1.75)% in solution of 1% sodium alginate and in combination of 5% dimethyl sulfoxide (DMSO) and 1% sodium alginate, respect tively). Using cryomicroscopy, the presence of sodium alginate in cryoprotective medium was shown to change the crystallization performance and to shift the phase transition zone towards lower temperatures. The beginning of phase transformations in the specimens, cooled in 5% DMSO solution was recorded at –8.5°C, in those, containing 1% sodium alginate and in a three-component system, comprising the combination of 1% sodium alginate and 5% DMSO, it was found at –16 and –15°С, respectively. Probl Cryobiol Cryomed 2018; 28(3): XXX–XXX

To evaluate the effects of glucagon-like peptide-1 analogs (GLP-1a) combined with insulin on myocardial ischemia-reperfusion injury in diabetic rats.Type 2 diabetes mellitus (T2DM) was induced in male Wistar rats with streptozotocin (65 mg/kg) and verified using an oral glucose tolerance test. After anesthesia, the left coronary artery was occluded for 40 min followed by 80 min reperfusion. Blood glucose level was measured during surgery. Rats were randomized into six groups as follows: (1) control rats; (2) insulin (0.1 U/kg) treated rats prior to ischemia; (3) insulin (0.1 U/kg) treated rats at reperfusion; (4) GLP-1a (140 mg/kg) treated rats prior to ischemia; (5) GLP-1a (140 mg/kg) treated rats at reperfusion; and (6) rats treated with GLP-1a (140 mg/kg) prior to ischemia plus insulin (0.1 U/kg) at reperfusion. Myocardial area at risk and infarct size was measured planimetrically using Evans blue and triphenyltetrazolium chloride staining, respectively.There was no significant difference in the myocardial area at risk among groups. Insulin treatment before ischemia resulted in a significant increase in infarct size (34.7% ± 3.4%GLP-1a pre-administration results in myocardial infarct size reduction in rats with T2DM. These effects are maximal in rats treated with GLP-1a pre-ischemia plus insulin at reperfusion.

The anodic oxidation of serine anion on smooth (Pt) and platinized (Pt(Pt)) platinum electrodes is studied by the methods of cyclic and linear voltammetry, rotating disk electrode, coulometry, and reflectance IR spectroscopy. On both electrodes, the potential regions of electrochemical transformation of this amino acid are determined. It is shown that electrooxidation of serine proceeds with abstraction of 4 and 2 electrons on Pt and Pt(Pt) electrodes, respectively. It is found that the anodic oxidation of serine anions proceeds from the adsorbed state; a possible kinetic scheme of this process is proposed.

Методами циклической линейной вольтамперометрии, вращающегося дискового электрода и кулонометрии, а также ИК-спектроскопии изучено влияние рН среды на процессы анодного окисления серина на платинированном платиновом электроде. Определены области потенциалов электрохимического превращения аминокислоты. Показано, что электроокисление серина происходит с участием 2 электронов в кислой и щелочной средах и 4 электронов – при окислении цвиттериона. Установлено, что вне зависимости от рН раствора анодное окисление серина осуществляется из адсорбированного состояния; предложена возможная кинетическая схема процесса.

A method of synthesis of silver glyconanoparticles with average particle size 15–40 nm and low polydispersity index based on available 3-thiopropionylhydrazones of mono- and disaccharides has been elaborated. The prepared compounds have exhibited high antifungal, antibacterial, and antiviral activity and are promising for further comprehensive study.

The accessible D-ribose mercaptoacetyl-, 3-mercaptopropionyl-, and 2-mercaprobenzoylhydrazones were used to develop a method of synthesis of gold glyconanoparticles with the average particle diameter of 10–40 nm and a low polydispersity index.

In their structure pyrimidin-4-one derivatives are similar to the endogenous nitrogen bases. This makes it possible to predict a wide range of pharmacological activities characteristic for them. The compounds of this series exhibit neurotropic, immunotropic, actoprotective, hypotensive, antihypoxic, anti-infl ammatory and antioxidant activity. Within this framework, the search for and creation of highly effective and safe anti-infl ammatory drugs in the series of N-arylpyrimidin-4(1H)-one is of great current interest. The aim of the work is to carry out predictive studies and targeted synthesis of N-substituted derivatives of pyrimidine-4(1H)-one with anti-infl ammatory activity, as well as toconfi rm the validity of their molecular construction by the results of pharmacological tests. Materials and methods. The research was carried out using the logical-structural approach and information technologies. The computer analysis of biological activity was carried out by the PASS program. Synthesis of the target compounds was carried out using a modifi ed procedure. The structure of the synthesized compounds was confi rmed by 1 H NMR, IR and UV spectroscopy. The investigation of the anti-infl ammatory effect of the synthesized compounds was carried out on the model of acute aseptic infl ammation. The synthesized substances were injected intraperitoneally, the magnitude of the edema was the criterion for evaluating the anti-infl ammatory activity. Results and discussion . In the course of the research on the basis of the logico-structural approach, hydroxyphenyl and alkyl derivatives of pyrimidin-4(1H)-one with anti-infl ammatory properties were validated. A preliminary analysis of the pharmacological properties of the predicted structures was carried out using the PASS program and the most promising compounds were selected. To synthesize the tolyl and hydroxyphenyl derivatives of pyrimidin-4(1H)-one, a modifi ed procedure was used. Its essence consists in the use of catalytic amounts of dimethylsulfoxide in order to increase the nucleophilicity of the amine component of the reaction. In order to confi rm the reliability and expediency of the molecular construction, the antiexudative activity of the target compounds was studied. Conclusion. The results of pharmacological studies indicate the prospect of searching for and creating new biologically active compounds having anti-infl ammatory activity among the tolyl and hydroxyphenyl derivatives of pyrimidin-4(1H)-one.