11 results on '"Klymov A"'
Search Results
2. A Radially Organized Multipatterned Device as a Diagnostic Tool for the Screening of Topographies in Tissue Engineering Biomaterials
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Joost teRiet, Manuela E. Gomes, John A. Jansen, X. Frank Walboomers, Rui L. Reis, Pedro S. Babo, Alexey Klymov, and Universidade do Minho
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0301 basic medicine ,Nanogrooves ,Materials science ,Periodontal Ligament ,Surface Properties ,0206 medical engineering ,Biomedical Engineering ,Medicine (miscellaneous) ,Biocompatible Materials ,Bioengineering ,02 engineering and technology ,03 medical and health sciences ,chemistry.chemical_compound ,Tissue engineering ,Cell Movement ,Anisotropic alignment ,Humans ,Nanotechnology ,Regeneration ,Platelet lysate ,Periodontal fiber ,Groove (engineering) ,Cells, Cultured ,Cell Proliferation ,Science & Technology ,Tissue Engineering ,Regeneration (biology) ,Other Research Radboud Institute for Health Sciences [Radboudumc 0] ,Biomaterial ,020601 biomedical engineering ,High-Throughput Screening Assays ,Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] ,030104 developmental biology ,chemistry ,Genipin ,Extracellular matrix organization ,Biomedical engineering - Abstract
Micro- and nanotextured biomaterial surfaces have been widely studied for their capacity to drive the regeneration of organized tissues. Nanotopographical features in the shape of grooveâ ridge patterns aim at mimicking the extracellular matrix organization. However, to date, a wide array of groove and ridge sizes has been described. In this work, we therefore tested a device composed of a multipatterned array consisting of six patterns of radially arranged parallel nanogrooves, with a pitch ranging from 0 to 1000nm and a depth ranging from 0 to 170 nm, to be used as a tool for the expeditious and simultaneous screening of surface topographies aiming the regeneration of anisotropically organized tissues such as ligament. The topographies were reproduced in (1) epoxy resin or (2) membranes produced by the crosslinking of platelet lysate (PL) with genipin (gPL). Both materials were seeded with periodontal ligament cells (PDLCs) and the proliferation, migration, as well as cell alignment were assessed. The effect of topography in PDLCs was only evident in terms of cell organization, resulting in a highly anisotropic organization of the cells for the 1000 and 600nm patterns, and in an increased isotropic organization for shallower topographies. Overall, our results suggest that this multipatterned system can be a valuable diagnostic tool for biomaterials aiming at the regeneration of anisotropically organized tissues, such as periodontal ligament., P.S.B. acknowledges Fundac¸a˜o para a Cieˆncia e Tecnologia (FCT) for the PhD Grant SFRH/BD/73403/2010. We thank the RIMLS Microscopic Imaging Center for the use of their facilities. J.t.R. is supported by a Dutch NWO Veni grant (680-47-421). This research received funding from an NWO Medium Sized Investment (NOW-ZonMV 91110007).
- Published
- 2016
3. Increased acellular and cellular surface mineralization induced by nanogrooves in combination with a calcium-phosphate coating
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X. Frank Walboomers, Alexey Klymov, Jiankang Song, Sander C.G. Leeuwenburgh, John A. Jansen, Joost te Riet, and Xinjie Cai
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Calcium Phosphates ,Microscopy, Electron, Scanning Transmission ,Bone sialoprotein ,Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2] ,Bone Morphogenetic Protein 2 ,02 engineering and technology ,Microscopy, Atomic Force ,Biochemistry ,Mineralization (biology) ,Mice ,0302 clinical medicine ,Coated Materials, Biocompatible ,Coating ,Osteogenesis ,MC3T3 ,biology ,Cell Differentiation ,3T3 Cells ,General Medicine ,021001 nanoscience & nanotechnology ,Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] ,Alkaline phosphatase ,Collagen ,0210 nano-technology ,Biotechnology ,Materials science ,Cell Survival ,Surface Properties ,Osteocalcin ,Biomedical Engineering ,chemistry.chemical_element ,Nanotechnology ,Calcium ,engineering.material ,Biomaterials ,03 medical and health sciences ,Calcification, Physiologic ,Animals ,Integrin-Binding Sialoprotein ,Viability assay ,Molecular Biology ,Cell Proliferation ,Osteoblasts ,Other Research Radboud Institute for Health Sciences [Radboudumc 0] ,Cell Membrane ,Substrate (chemistry) ,030206 dentistry ,Alkaline Phosphatase ,Nanostructures ,chemistry ,Solvents ,biology.protein ,engineering ,Biophysics ,Osteopontin - Abstract
Item does not contain fulltext The current work evaluated the influence of nanoscale surface-topographies in combination with a calcium phosphate (CaP) coating on acellular and cellular surface mineralization. Four groups of substrates were produced, including smooth, grooved (940nm pitch, 430nm groove width, 185nm depth), smooth coated, and grooved coated. The substrates were characterized by scanning/transmission electron microscopy and atomic force microscopy. Osteoblast-like MC3T3 cells were cultured on the substrates for a period up to 35days under osteogenic conditions. Differentiation was observed by alkaline phosphatase assay and PCR of collagen I (COLI), osteopontin (OPN), osteocalcin (OC), bone-morphogenic protein 2 (BMP2), and bone sialoprotein (BSP). Mineralization was quantified by a calcium assay and Alizarin Red staining. In addition, acellular mineralization was determined after incubation of substrates in just cell culture medium without cells. Results showed that a reproducible nano-metric ( approximately 50nm) CaP-layer could be applied on the substrates, without losing the integrity of the topographical features. While no relevant differences were found for cell viability, cells on smooth surfaces proliferated for a longer period than cells on grooved substrates. In addition, differentiation was affected by topographies, as indicated by an increased expression of OC, OPN and ALP activity. Deposition of a CaP coating significantly increased the acellular mineralization of smooth as well grooved substrate-surfaces. However, this mineralizing effect was strongly reduced in the presence of cells. In the cell seeded situation, mineralization was significantly increased by the substrate topography, while only a minor additive effect of the coating was observed. In conclusion, the model presented herein can be exploited for experimental evaluation of cell-surface interaction processes and optimization of bone-anchoring capability of implants. The model showed that substrates modified with CaP-coated coated nanogrooves display enhanced in vitro mineralization as compared to unmodified controls or substrates modified with either nanogrooves or CaP coatings. However, our results also indicated that acellular mineralization assays are not necessarily predictive for biological performance. STATEMENT OF SIGNIFICANCE: The manuscript describes the possibility to combine the mechanical properties of nanosized topographies with the biochemical properties of a calcium phosphate based coating for improvement of surface mineralization. Interestingly, our results demonstrate that further incubation of our surfaces in SBF type media allowed all surfaces to mineralize rapidly to a high extent. Moreover we prove that nanotexture be used to can stimulate and organize mineralization and that the combination surface of a CaP coating and a nanotexture has the potential to be effective as a bone-implant surface. Such experiments will be of considerable interest to those in the research community and industry, who are focusing on bio-mineralization processes and optimization of modern bone-implants. 01 februari 2016
- Published
- 2016
4. Chemical composition and some structural properties of Zn1-x MnxS semiconductor films obtained by closespaced vacuum sublimation
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D. Kurbatov, O. Klymov, and A. Frolov
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Lattice constant ,Semiconductor ,Vacuum sublimation ,Materials science ,chemistry ,Scattering ,business.industry ,Analytical chemistry ,chemistry.chemical_element ,Manganese ,Substrate (electronics) ,business ,Chemical composition - Abstract
We report on the evaluation of coherent scattering domain sizes, microstrains, microstresses, and average density of dislocations in Zn 1-x Mn x S films deposited close-spaced vacuum sublimation method with different substrate temperature. It was established that the introduction of manganese leads to some improvement of substructural characteristics of condensates compared to nondoped layers.
- Published
- 2018
5. Monitoring local delivery of vancomycin from gelatin nanospheres in zebrafish larvae
- Author
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Sander C.G. Leeuwenburgh, Yang Zhang, Fang Yang, John A. Jansen, Sebastian A. J. Zaat, Alexey Klymov, Xiaolin Zhang, Jiankang Song, Jeroen J.J.P. van den Beucken, Leonie de Boer, Biomaterials Science and Technology, Graduate School, and Medical Microbiology and Infection Prevention
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Pharmaceutical Science ,02 engineering and technology ,medicine.disease_cause ,01 natural sciences ,Gelatin ,Drug Delivery Systems ,Biodistribution ,International Journal of Nanomedicine ,Drug Discovery ,Tissue Distribution ,Zebrafish ,Fluorescence microscopy ,biology ,Chemistry ,In vivo real-time monitoring ,General Medicine ,Staphylococcal Infections ,021001 nanoscience & nanotechnology ,Endocytosis ,Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] ,Staphylococcus aureus ,Larva ,Injections, Intravenous ,Vancomycin ,0210 nano-technology ,Nanospheres ,Intracellular ,medicine.drug ,animal structures ,food.ingredient ,Green Fluorescent Proteins ,Biophysics ,Bioengineering ,010402 general chemistry ,Injections, Intramuscular ,Microbiology ,Biomaterials ,food ,All institutes and research themes of the Radboud University Medical Center ,In vivo ,medicine ,Animals ,Humans ,Fluorescent Dyes ,Bacteria ,Macrophages ,fungi ,Organic Chemistry ,Cell-material interaction ,biology.organism_classification ,Survival Analysis ,In vitro ,0104 chemical sciences ,Intracellular infection - Abstract
Xiaolin Zhang,1,2,* Jiankang Song,3,* Alexey Klymov,3,* Yang Zhang,3 Leonie de Boer,1 John A Jansen,3 Jeroen JJP van den Beucken,3 Fang Yang,3 Sebastian AJ Zaat,1,* Sander CG Leeuwenburgh3,* 1Department of Medical Microbiology, Amsterdam Infection and Immunity Institute, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands; 2Department of Biomaterials Science and Technology, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, the Netherlands; 3Department of Biomaterials, Radboud University Medical Centre, Nijmegen, the Netherlands *These authors contributed equally to this work Background: Infections such as biomaterial-associated infection and osteomyelitis are often associated with intracellular survival of bacteria (eg, Staphylococcus aureus). Treatment of these infections remains a major challenge due to the low intracellular efficacy of many antibiotics. Therefore, local delivery systems are urgently required to improve the therapeutic efficacy of antibiotics by enabling their intracellular delivery. Purpose: To assess the potential of gelatin nanospheres as carriers for local delivery of vancomycin into macrophages of zebrafish larvae in vivo and into THP-1-derived macrophages in vitro using fluorescence microscopy. Materials and methods: Fluorescently labeled gelatin nanospheres were prepared and injected into transgenic zebrafish larvae with fluorescent macrophages. Both the biodistribution of gelatin nanospheres in zebrafish larvae and the co-localization of vancomycin-loaded gelatin nanospheres with zebrafish macrophages in vivo and uptake by THP-1-derived macrophages in vitro were studied. In addition, the effect of treatment with vancomycin-loaded gelatin nanospheres on survival of S. aureus-infected zebrafish larvae was investigated. Results: Internalization of vancomycin-loaded gelatin nanospheres by macrophages was observed qualitatively both in vivo and in vitro. Systemically delivered vancomycin, on the other hand, was hardly internalized by macrophages without the use of gelatin nanospheres. Treatment with a single dose of vancomycin-loaded gelatin nanospheres delayed the mortality of S. aureus-infected zebrafish larvae, indicating the improved therapeutic efficacy of vancomycin against (intracellular) S. aureus infection in vivo. Conclusion: The present study demonstrates that gelatin nanospheres can be used to facilitate local and intracellular delivery of vancomycin. Keywords: in vivo real-time monitoring, fluorescence microscopy, biodistribution, cell-material interaction, Staphylococcus aureus, intracellular infection
- Published
- 2018
6. Nanostructured raspberry-like gelatin microspheres for local delivery of multiple biomolecules
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Alexey Klymov, Hajar Seyednejad, Bram Pape, Jiankang Song, Dennis W. P. M. Löwik, Sander C.G. Leeuwenburgh, Mani Diba, and Yang Zhang
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Materials science ,food.ingredient ,Systems Chemistry ,Biomedical Engineering ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Gelatin ,Biomaterials ,Mice ,Colloid ,Drug Delivery Systems ,food ,Animals ,Humans ,Molecular Biology ,chemistry.chemical_classification ,Supercritical carbon dioxide ,Biomolecule ,General Medicine ,Carbon Dioxide ,021001 nanoscience & nanotechnology ,Microspheres ,Supercritical fluid ,0104 chemical sciences ,Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] ,chemistry ,Drug delivery ,NIH 3T3 Cells ,Nanoparticles ,0210 nano-technology ,Dispersion (chemistry) ,Biotechnology - Abstract
Contains fulltext : 177168.pdf (Publisher’s version ) (Closed access) Multicompartment particles, which are particles composed of smaller building units, have gained considerable interest during the past decade to facilitate simultaneous and differential delivery of several biomolecules in various applications. Supercritical carbon dioxide (CO2) processing is an industrial technology widely used for large-scale synthesis and processing of materials. However, the application of this technology for production of multicompartment particles from colloidal particles has not yet been explored. Here, we report the formation of raspberry-like gelatin (RLG) microparticles composed of gelatin nanoparticles as colloidal building blocks through supercritical CO2 processing. We show that these RLG microparticles exhibit a high stability upon dispersion in aqueous media without requiring chemical cross-linking. We further demonstrate that these microparticles are cytocompatible and facilitate differential release of two different model compounds. The strategy presented here can be utilized as a cost-effective route for production of various types of multicompartment particles using colloidal particles with suitable interparticle interactions. STATEMENT OF SIGNIFICANCE: Multicompartment particles have gained considerable interest during the past decade to facilitate simultaneous and differential delivery of multiple biomolecules in various biomedical applications. Nevertheless, common methods employed for the production of such particles are often complex and only offer small-scale production. Here, we report the formation of raspberry-like gelatin (RLG) microparticles composed of gelatin nanoparticles as colloidal building blocks through supercritical CO2 processing. We show that these microparticles are cytocompatible and facilitate differential release of two model compounds with different molecular sizes, promising successful applications in various biomedical areas. Summarizing, this paper presents a novel strategy that can be utilized as a cost-effective route for production of various types of multicompartment particles using a wide range of colloidal building blocks.
- Published
- 2017
7. Studying the elemental composition and manganese distribution in Zn1 − x Mn x Te and Zn1 − x Mn x S films using the μ-PIXE and EDAX methods
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D. I. Kurbatov, S. S. Mel’nik, A. V. Klymov, A. S. Opanasyuk, and O. G. Ponomarev
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Elemental composition ,Microprobe ,Materials science ,chemistry ,Analytical chemistry ,chemistry.chemical_element ,Chemical vapor deposition ,Manganese ,Areal distribution ,Thin film ,Surfaces, Coatings and Films ,Solid solution - Abstract
The methods of proton-induced X-ray emission (μ-PIXE) and energy-dispersive X-ray analysis (EDAX) are used to study the elemental composition and areal distribution of manganese in Zn1 − xMnxTe and Zn1 − xMnxS films. The μ-PIXE technique is implemented using a nuclear scanning microprobe with a proton-beam energy of 1.5 MeV and cross-sectional dimensions of 4 × 4 μm. The semimagnetic solid solution films are obtained under different operating modes of thermal vapor deposition in the quasiclosed volume of a manganese-containing blend.
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- 2014
8. Mineralization and bone regeneration using a bioactive elastin-like recombinamer membrane
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Alexey Klymov, X. Frank Walboomers, J. Carlos Rodríguez-Cabello, M. Reza Nejadnik, Esther Tejeda-Montes, Alvaro Mata, and Matilde Alonso
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Calcium Phosphates ,Male ,Bone Regeneration ,Materials science ,Molecular Sequence Data ,Biophysics ,chemistry.chemical_element ,Biocompatible Materials ,Bioengineering ,Calcium ,Mineralization (biology) ,Rats, Sprague-Dawley ,Biomaterials ,Osteogenesis ,Cell Adhesion ,Animals ,Amino Acid Sequence ,Salivary Proteins and Peptides ,Cell adhesion ,Bone regeneration ,Cells, Cultured ,Tissue Scaffolds ,biology ,Mesenchymal stem cell ,Cell Differentiation ,Membranes, Artificial ,Mesenchymal Stem Cells ,Recombinant Proteins ,Elastin ,Rats ,Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] ,Membrane ,chemistry ,Mechanics of Materials ,Bone Substitutes ,Ceramics and Composites ,biology.protein ,Alkaline phosphatase ,Biomedical engineering - Abstract
Item does not contain fulltext The search for alternative therapies to improve bone regeneration continues to be a major challenge for the medical community. Here we report on the enhanced mineralization, osteogenesis, and in vivo bone regeneration properties of a bioactive elastin-like recombinamer (ELR) membrane. Three bioactive ELRs exhibiting epitopes designed to promote mesenchymal stem cell adhesion (RGDS), mineralization (DDDEEKFLRRIGRFG), and both cell adhesion and mineralization were synthesized using standard recombinant protein techniques. The ELR materials were then used to fabricate membranes comprising either a smooth surface (Smooth) or channel microtopographies (Channels). Mineralization and osteoblastic differentiation of primary rat mesenchymal stem cells (rMSCs) were analyzed in both static and dynamic (uniaxial strain of 8% at 1 Hz frequency) conditions. Smooth mineralization membranes in static condition exhibited the highest quantity of calcium phosphate (Ca/P of 1.78) deposition with and without the presence of cells, the highest Young's modulus, and the highest production of alkaline phosphatase on day 10 in the presence of cells growing in non-osteogenic differentiation medium. These membranes were tested in a 5 mm-diameter critical-size rat calvarial defect model and analyzed for bone formation on day 36 after implantation. Animals treated with the mineralization membranes exhibited the highest bone volume within the defect as measured by micro-computed tomography and histology with no significant increase in inflammation. This study demonstrates the possibility of using bioactive ELR membranes for bone regeneration applications.
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- 2014
9. Nanometer-grooved topography stimulates trabecular bone regeneration around a concave implant in a rat femoral medulla model
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Johannes G.E. Gardeniers, X. Frank Walboomers, Peter Mulder, Alexey Klymov, John A. Jansen, Joost te Riet, Faculty of Science and Technology, and Mesoscale Chemical Systems
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Nanogrooves ,Materials science ,Rat femoral condyle ,Surface Properties ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,chemistry.chemical_element ,Bioengineering ,Nanotopography ,02 engineering and technology ,Osseointegration ,03 medical and health sciences ,0302 clinical medicine ,Surface roughness ,medicine ,Animals ,Nanotechnology ,Regeneration ,General Materials Science ,Femur ,Bone regeneration ,Titanium ,Bone implants ,Other Research Radboud Institute for Health Sciences [Radboudumc 0] ,Prostheses and Implants ,030206 dentistry ,Anatomy ,021001 nanoscience & nanotechnology ,Rats ,Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] ,medicine.anatomical_structure ,chemistry ,Cancellous Bone ,Molecular Medicine ,Implant ,0210 nano-technology ,Cancellous bone ,Biomedical engineering - Abstract
Contains fulltext : 167419.pdf (Publisher’s version ) (Closed access) In the present study, a method was developed to reproduce two nanogrooved patterns (groove width/ridge width/depth: 150/150/50 nm and 200/800/70 nm) into cylindrical epoxy resin implants, which were subsequently coated with 20 nm of titanium. Also, implants with a conventional surface roughness (Rq=1.6 mum) were produced. After cytocompatibility analysis of the produced surfaces, implants were installed into the femoral condyle of rats for 4 and 8 weeks. The histomorphometrical analysis of bone volume in a 100 mum wide zone close to the implant surface showed that only for the 200/800 grooves the amount of bone increased significantly between 4 and 8 weeks of implantation. In addition, at the late time point only implants with the 200/800 pattern revealed a significantly higher bone volume compared to the rough controls. In conclusion, the 200/800 grooved pattern can positively influence bone volume adjacent to the implant surface, and should be evaluated and optimized in further (pre-)clinical studies.
- Published
- 2016
10. Optimization of phosphorous diffusion in silicon photocells
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Maksym Klymov
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Materials science ,Organic solar cell ,Silicon ,Natural resource economics ,business.industry ,chemistry.chemical_element ,Hybrid solar cell ,Quantum dot solar cell ,Polymer solar cell ,law.invention ,Monocrystalline silicon ,chemistry ,law ,Solar cell ,ComputerSystemsOrganization_SPECIAL-PURPOSEANDAPPLICATION-BASEDSYSTEMS ,Plasmonic solar cell ,Process engineering ,business - Abstract
Today the main goals for photovoltaic industry are improvement of solar cell parameters and decreasing its cost. Author describes an experiment in manufacturing conditions that proves satisfactory results and achieves the both mentioned goals of photovoltaic industry.
- Published
- 2013
11. Large-Scale Synthesis and Modifications of Bicyclo[1.1.1]pentane-1,3-dicarboxylic Acid (BCP)
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Pavel K. Mykhailiuk, Vasyl Ripenko, Serhii Zhersh, Daniil Vysochyn, and Ivan Klymov
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chemistry.chemical_classification ,Diketone ,Bicyclic molecule ,010405 organic chemistry ,Organic Chemistry ,Haloform reaction ,010402 general chemistry ,01 natural sciences ,Diacetyl ,Article ,0104 chemical sciences ,Amino acid ,Pentane ,Propellane ,chemistry.chemical_compound ,Dicarboxylic acid ,chemistry ,Alcohols ,Pentanes ,Organic chemistry ,Dicarboxylic Acids ,Amines ,Amino Acids - Abstract
In flow photochemical addition of propellane to diacetyl allowed construction of the bicyclo[1.1.1]pentane (BCP) core in a 1 kg scale within 1 day. Haloform reaction of the formed diketone in batch afforded bicyclo[1.1.1]pentane-1,3-dicarboxylic acid in a multigram amount. Representative gram scale transformations of the diacid were also performed to obtain various BCP-containing building blocks—alcohols, acids, amines, trifluoroborates, amino acids, etc.—for medicinal chemistry.
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