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Your search keyword '"Katia Guerrini"' showing total 2 results
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2 results on '"Katia Guerrini"'

1. Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain

Author

Katia Guerrini, Veniero Gambaro, Fiorenza Farè, Argo A, Cristina Borroni, Gabriella Roda, Lucia Dell’Acqua, Daria Catalano, Paolo Procaccianti, Guerrini, K, Argo, A, Borroni, C, Catalano, D, Dell’Acqua, L, Farè, F, Procaccianti,P, Roda,G, and Gambaro,V

Subjects
Adult, Male, Clinical Biochemistry, Analytical chemistry, Pharmaceutical Science, Hippocampus, Gas Chromatography-Mass Spectrometry, Analytical Chemistry, Heroin, chemistry.chemical_compound, Settore MED/43 - Medicina Legale, Limit of Detection, Drug Discovery, medicine, Humans, Tissue Distribution, Derivatization, Spectroscopy, Morphine, Codeine, Post-mortem brain specimen, Chromatography, Molecular Structure, Heroin Dependence, Illicit Drugs, Brain, Reproducibility of Results, Pons, Substance Abuse Detection, chemistry, Frontal lobe, Drug Overdose, Occipital lobe, medicine.drug
Abstract

Brain distribution pattern of “street” heroin metabolites (morphine and codeine) was investigated in two fatalities due to “acute narcotism”. A suitable sample pretreatment prior to solid-phase-extraction was developed to achieve a good recovery of the analytes and to eliminate the interfering species. After derivatization with MSTFA, samples were analyzed by GC/MS. Specificity, accuracy, precision and linearity of the method were evaluated; LOD and LOQ were, respectively, 10 ng/25 ng for morphine and 5 ng/10 ng for codeine. This method was applied to the analysis of six brain areas (hippocampus, frontal lobe, occipital lobe, nuclei, bulb and pons) coming from two cases of heroin-related deaths. No evidence of accumulation of metabolites in a specific brain region was found.

Published
2012

2. Determination of the active principles of Catha edulis: Quali–quantitative analysis of cathinone, cathine, and phenylpropanolamine

Author

Gabriella Roda, Sebastiano Arnoldi, Veniero Gambaro, Maria Laura Colombo, Katia Guerrini, and Lucia Dell’Acqua

Subjects
Flame Ionization, Chromatography, Cathinone, Plant Stems, Chemistry, Phenylpropanolamine, Forensic toxicology, Catha, Quantitative determination, Gas Chromatography-Mass Spectrometry, Pathology and Forensic Medicine, Plant Leaves, Forensic Toxicology, Alkaloids, Maceration (wine), medicine, Central Nervous System Stimulants, Gas chromatography–mass spectrometry, Desiccation, Law, Quantitative analysis (chemistry), Cathine, medicine.drug
Abstract

In the last years, all the vegetable material supposed to belong to the Catha Edulis species, seized at the Malpensa and Orio al Serio airports, were analyzed in our laboratory on behalf of the Tribunals of Busto Arsizio and Bergamo, respectively. After a preliminary botanic examination, the quali-quantitative determination of the active principles cathinone, cathine and phenylpropanolamine (PPA) was carried out by means of GC/MS and GC/FID techniques, which meet the requirements of the forensic analyses. We developed a fast, effective and reliable derivatization procedure which allowed to simultaneously detect cathine and PPA, whose discrimination is mandatory since PPA is not a psychoactive agent. Cathine was distributed in the various parts of the plant (leaves and stems) and its quantity ranged from 0.03% to 0.17% of the weight of the vegetable material; PPA was not detected in the twigs and its quantity in the leaves ranged from 0.07% to 0.16%. The quantitative determination of cathinone was carried out directly on the methanol solution after maceration of the vegetable material, its quantity ranging from 0.02% to 0.10%. No significant difference in the content of the two active principles was found between the fresh and the dried material.

Published
2012

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