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As a promising alternative to petroleum fossil energy, polymer electrolyte membrane fuel cell has drawn considerable attention due to its low pollution emission, high energy density, portability, and long operation times. Proton exchange membrane (PEM) like Nafion plays an essential role as the core of fuel cell. A good PEM must have satisfactory performance such as high proton conductivity, excellent mechanical strength, electrochemical stability, and suitable for making membrane electrode assemblies (MEA). However, performance degradation and high permeability remain the main shortcomings of Nafion. Therefore, the development of a new PEM with better performance in some special conditions is greatly desired. In this review, we aim to summarize the latest achievements in improving the Nafion performance that works well under elevated temperature or methanol-fueled systems. The methods described in this article can be divided into some categories, utilizing hydrophilic inorganic material, metal-organic frameworks, nanocomposites, and ionic liquids. In addition, the mechanism of proton conduction in Nafion membranes is discussed. These composite membranes exhibit some desirable characteristics, but the development is still at an early stage. In the future, revolutionary approaches are needed to accelerate the application of fuel cells and promote the renewal of energy structure.

Supported NiCu bimetallic catalysts have been produced in-situ on commercial Al2O3 by using layered double hydroxides as precursors. The resulting catalysts show a uniform Ni and Cu distribution, thus providing good activity and selectivity in the reforming reaction of n-heptane. The catalytic performance has been found to depend on the Cu/Ni ratio, revealing the synergic catalysis between homogeneously dispersed Ni and Cu sites. The good catalysis of NiCu bimetallic catalysts makes it possible to partly or even completely replace Pt with NiCu bimetallic catalysts.

Background and purpose Neuropathic pain is a widespread health problem with limited curative treatment. Decreased sarco/endoplasmic reticulum Ca2+ -ATPase (SERCA) expression has been reported in dorsal root ganglion (DRG) of animals suffering from neuropathic pain. We aimed to establish the relationship between SERCA expression and the pain responses and to elucidate the underlying molecular mechanism. Experimental approach Neuropathic pain was modeled using rat chronic constriction injury (CCI). Ca2+ imaging and current clamp patch-clamp were used to determine cytosolic Ca2+ levels and action potential firing, respectively. Western blots, immunofluorescence staining and RT-PCR were used to quantitatively assess protein and mRNA expression, respectively. H&E staining and coupled enzyme assay were used to evaluate the nerve injury and SERCA2b activity, respectively. Key results SERCA2b is the predominant SERCA isoform in rat DRG and its expression is decreased after CCI at mRNA, protein and activity levels. Whereas inhibiting SERCA with thapsigargin causes neuronal hyperexcitation, nerve injury, ER stress, satellite glial cell activation and mechanical allodynia, activating SERCA by CDN1163 or overexpressing SERCA2b in DRG after CCI produces long-term relief of mechanical and thermal allodynia with accompanied morphological and functional restoration through alleviation of ER stress. Furthermore, the downregulation of DRG SERCA2b in CCI rats is caused by increased production of reactive oxygen species (ROS) through Sp1-dependent transcriptional inhibition. Conclusion and implications Our findings reveal a novel pathway centering around SERCA2b as the key molecule underlying the mechanism of development and maintenance of neuropathic pain, and SERCA2b activators have the potential for therapeutic treatment of neuropathic pain.

An effective decontamination method for soils remediation is a long-standing challenge. Herein, hydrogen peroxide (H2O2) was spontaneously produced in thin films generated by evaporating almost all the water. H2O2-sensitive test strips and oxidation of 2-hydroxybenzophenone to 2-hydroxyphenyl benzoate confirmed the generation of H2O2. Experiments in which reaction time and temperature were varied and established dependence of H2O2 production on the long-lasting thin films. H2O2 production was independence of irradiation and oxygen from air or dissolved oxygen. Spin-trapping probe experiments suggested that electric fields at the thin interfacial films enabled OH- to hydroxyl radicals and further recombination to H2O2. As a proof-of-principle experiment, perfluorooctanoic acid (PFOA) was effectively degraded on the thin films without catalysts and UV irradiation could further facilitate the degradation. A degradation mechanism was proposed involving the PFOA radicals on thin interfacial films and the subsequent decomposition of the radicals with successive loss of CF2. These findings have important implications for the development of decontamination method for soils remediation based on thin films, because thin film formats are ubiquitous at the surface of soils and when aerosols falling onto a substrate.

In this work, a novel MXene-copolymeric molecularly imprinted hydrogel (FMMIH) with temperature/pH dual response and photothermal conversion performance for selective recognition of cis-diol compounds is successfully prepared. Functionalized MXene becomes an important part of the hydrogel’s skeleton by participating in the free radical polymerization reaction. Therefore, FMMIH exhibits improved mechanical properties and great photothermal conversion performance. Furthermore, based on the temperature/pH dual response, FMMIH can realize the controllable capture and release of ginsenoside Rb1 with cis diol structures through dual recognitions of functional groups and geometric space. Under the conditions of pH 8.5 and 25 °C, the adsorption capacity of FMMIH is 26.3 mg·g−1 and the imprinting factor is 16.4, showing a good imprinting effect. It is worth noting that the volume shrinkage caused by photothermal conversion of the hydrogel is conducive to the elution of template molecules and greatly saves the time of desorption (the desorption efficiency is as high as 89.4% under light conditions for 30 min, which is 3.7 times in dark conditions). These excellent properties make it possible to have broad prospects in many fields such as separation of cis-diol compounds, drug delivery systems, and biosensors.

Effective dye separation and desalination are critical for the treatment of highly saline textile wastewater with dye mixtures. In this study, a graphene oxide (GO) membrane with a tunable interlayer distance (d) was fabricated to generate clean water via two-stage filtration, namely, the dye/salt separation and desalination stages. In the first stage, under low pressure (e.g., 0.3 MPa), the membrane with a d value of ca. 7.60 A was suitable for removing the dye from the saline wastewater. The dye and salt (i.e., Na2SO4) rejection rates of >99% and

Circular RNAs (circRNAs) was suggested to play crucial regulatory roles in various human diseases, including Parkinson's disease (PD). This research aimed to investigate the function and potential mechanism of circ_0070441 in PD. MPP+ (1-methyl-4-phenylpyridinium)-treated SH-SY5Y cells was used as an in vitro cellular PD model. The expressions of circ_0070441, microRNA (miR)-626 and insulin receptor substrate 2 (IRS2) were measured by quantitative real-time polymerase chain reaction (RT-qPCR) or western blot. Cell Counting Kit-8 (CCK-8) assay, Cytotoxicity Detection Kit (Lactate Dehydrogenase), flow cytometry and Caspase-3 Assay Kit were used to detect cell viability, LDH release, cell apoptosis and caspase-3 activity, respectively. The levels of inflammation-related factors were detected by enzyme-linked immunosorbent assay (ELISA). The correlation among circ_0070441, miR-626 and IRS2 were confirmed by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay. The levels of circ_0070441 and IRS2 were increased while miR-626 expression was decreased in MPP+-treated SH-SY5Y cells in dose- and time-dependent manners. Depletion of circ_0070441 alleviated MPP+-triggered neuronal damage by regulating cell apoptosis and inflammation. Circ_0070441 acted as a sponge for miR-626, and IRS2 was a target of miR-626. Besides, the neuroprotective effects of circ_0070441 knockdown or miR-626 overexpression were partly overturned by the suppression of miR-626 or IRS2 overexpression. Moreover, circ_0070441 upregulated IRS2 expression by interacting with miR-626. In summary, circ_0070441 aggravated MPP+-triggered neurotoxic effect in SH-SY5Y cells by regulating miR-626/IRS2 axis.

Revealing the modification inhomogeneity at the molecular level is important to understand the structure–property relationship for polysaccharide derivatives. Herein, we report a study to uncover t...

A direct C–H halogenation of 3-aryl-1H-pyrazol-5-amines using N-halogenosuccinimides (NXS; X = Br, I, Cl) in dimethyl sulfoxide at room temperature has been developed. This transformation provides an effective metal-free protocol for the synthesis of novel 4-halogenated pyrazole derivatives with moderate to excellent yields. The method utilizes NXS reagents as cheap and safe halogenating reagents under simple and mild reaction conditions, it has broad substrate scope, and can be used for gram-scale synthesis. The utility of this procedure is established by further transformations of the 4-halogenated products. Mechanism studies show that DMSO plays a dual role of catalyst and solvent.

Significant progress has been made on understanding the critical role of organic components in directing the collagen mineralization. We hypothesize that the inorganic trace elements might also play important role in the mineralization of collagenous matrix. To this aim, we systematically compared the in-vitro biomineralization behaviors of gelatin, gelatin-HA and gelatin-SiHA electrospun membranes. The results indicated that the presence of Si ions played a striking influence on the nucleation behaviors and mineralized structures. The gelatin-SiHA samples demonstrated more homogeneous nucleation within the gelatin fiber and growth along the fiber direction, in comparison with the heterogeneous nucleation and growth of spherulitic clusters on top of the nanofiber surface, i.e. extrafibrillar mineralization. The likely shift of the nucleation mode to the intrafibrillar mineralization in the presence of Si ions led to good alignment of apatite c-axis with the long axis of the nanofiber, resulting in a mineralization process and microstructure that were closer to those in natural bone. Cellular response analysis indicated that Si incorporation improved the MSC attachment and cytoskeleton organization. Such findings might have important implication in both understanding the complex mechanisms involved in collagen mineralization and optimal designing of advanced bio-inspired materials with potential superior mechanical and biological properties.

An efficient TBAI-catalyzed ring-opening sulfonylation of benzo[d]thiazole and arylsulfonyl hydrazide has been developed. Various benzo[d]thiazole as good thiol surrogates are compatible with the catalytic conditions, providing diversified unsymmetrical thiosulfonates with good yields. This strategy features mild reaction conditions, broad substrate scope, readily available starting materials, and gram-scale synthesis. Importantly, these products can be readily converted to novel o-amino-substituted diaryl sulfides.

Compared with chemically synthesized materials, biomass materials often exhibit unique intrinsic structures, which can be utilized through optimizing microstructures and activation process to furnish low-cost and efficient Microwave Absorption Materials. This research uses a common hydrophyte (Cyperus malaccensis Lam. var. brevifolius Bocklr) in South China as the raw material to fabricate carbon-based biomass-derived absorbers through one-step synthesis. The fabricated carbon materials show efficient Microwave Absorption (MA) performance with minimum reflection loss (RLmin) of − 26.0 dB at 11.9 GHz from the sample pyrolyzed at 700 °C under the thickness of only 2 mm and wide effective absorption bandwidth of 4.6 GHz (11.6–16.2 GHz) from the sample annealed at 600 °C. Moreover, the effective MA bandwidth can cover the entire C to Ku band by tuning the thickness. A balance of dielectric loss mechanism and impedance matching is the reason why this biomass carbon-based absorber exhibits excellent MA performance. The results show that this biomass carbon has great potential to be low-cost, green and efficient carbon-based microwave absorbers.

Although it is known that stresses on females damage oocytes with increased production of stress hormones, whether corticotrophin-releasing hormone (CRH) or adrenocorticotropic hormone (ACTH) harm oocytes directly are largely unknown. We demonstrated that CRH exposure during in vitro maturation impaired competence of both pig and mouse cumulus-oocyte-complexes (COCs), and it impaired competence and induced apoptosis in pig cumulus-denuded oocytes (DOs) but not in mouse DOs. CRH receptor 1 was expressed in pig DOs and in cumulus cells (CCs) of both species but not in mouse DOs. In the presence of CRH, whereas mouse CCs underwent apoptosis, pig CCs did not. While pig CCs did, mouse CCs did not express CRH-binding protein. ACTH did not affect competence of either pig or mouse COCs or DOs although they all expressed ACTH receptor. Both pig and mouse CCs expressed steroidogenic acute regulatory protein (StAR), and ACTH enhanced their progesterone production while alleviating their apoptosis. Neither pig nor mouse DOs expressed StAR, but ACTH inhibited maturation-promoting factor and decelerated meiotic progression of DOs suggesting activation of protein kinase A (PKA). In conclusion, CRH impaired pig and mouse oocyte competence by interacting with CRH receptor and inducing CCs apoptosis, respectively. ACTH activated PKA in both DOs and CCs although it showed no effect on oocyte competence.

Catalytic enantioselective epoxidation of olefins plays an important role in the production of optically-active epoxy. Transition-metal complexes prove efficient for the catalytic epoxidation of un-functionalized olefins by employing privileged chiral ligands with rotational symmetry and bulky substituents, which usually require complicated multistep synthesis and are often very expensive. Here, this work proposes an efficient strategy to promote the enantioselectivity of Mn(III)-catalyzed indene epoxidation, which simply utilizes the nanosheets of layered double hydroxides (LDHs) to modify α-amino acid derivatives as chiral ligands, achieving an enantioselectivity of more than 91.0%, much higher than that (

Studies have shown that the construction of a three-dimensional interconnected filler network is an effective method to improve the thermal conductivity of the through-plane under a lower filler load. However, huge challenges still exist in building a long-range layered filler network and reducing the thermal resistance resulting from contact between fillers and matrix. In this work, boron nitride nanosheets (BNNSs) were proposed to be connected with modified cellulose nanofibers (CNFs) to obtain a long-range layered structure filler skeleton by bidirectional freezing orientation. Then epoxy resin was dipped under vacuum condition to prepare composite with thermal conductivity up to 1.43 W/mK in through-plane when filler content is 4.3 vol%, and the composite had low thermal expansion coefficient of 64.1 ppm/°C and excellent volume resistivity up to 3.7 × 1012 Ω cm at the same time.

Increasing the work function of borophene over a large range is crucial for the development of borophene-based anode materials for highly efficient electronic devices. In this study, the effect of fluorine adsorption on the structures and stabilities, particularly on the work function, of α-borophene (BBP), was systematically investigated via first-principles density functional theory. The calculations indicated that BBP was well-stabilized by fluorine adsorption and the work functions of metallic fluorine-adsorbed BBPs (Fn-BBPs) sharply increased with increasing fluorine content. Moreover, the work function of F-BBP was close to that of the frequently used anode material Au and even, for other Fn-BBPs, higher than that of Pt. Furthermore, we have comprehensively discussed the factors, including substrate deformation, charge transfer, induced dipole moment, and Fermi and vacuum energy levels, affecting the improvement of work function. Particularly, we have demonstrated that the charge redistribution of the substrate induced by the bonding interaction between fluorine and the matrix predominantly contributes to the observed increase in the work function. Additionally, the effect of fluorine adsorption on the increase in the work function of BBP was significantly stronger than that of silicene or graphene. Our results concretely support the fact that Fn-BBPs can be extremely attractive anode materials for electronic device applications.

A TBAI-mediated sulfenylation of N,3-diaryl-1-arylsulfonyl-1H-pyrazol-5-amines with arylsulfonyl hydrazides has been established, and an expanded inventory of N,5-diaryl-4-(arylthio)-1H-pyrazol-3-amines was constructed through C–S bond formation and N–S bond breaking. Mechanistic investigations suggest thiosulfonate as a key intermediate in the sulfenylation, and the detosylation is promoted by the generated arylsulfinic acid. The method is characterized by simple operating conditions, broad substrate range as well as gram-scale reaction.

Selective dehydrogenation of ethanol under mild conditions is of great significance for the conversion of ethanol into high-value chemicals. This work reports Au/anatase–rutile TiO2 photocatalysts ...

M2-polarized macrophages are one of critical factors in tumour chemoresistance. An increasing number of studies have shown that M2 macrophage polarization can be promoted by chemoresistance. A large number of evidences indicate that Bufalin has significant antitumour effect, previous studies have found that Bufalin can reduce the polarization of M2 macrophages to play an anti-tumour effect in vivo, but the mechanism remains unclear. In our study, we found that Bufalin reduced the polarization of M2 macrophages induced by chemoresistant cells both in vivo and in vitro; however, Bufalin had no obvious direct effect on M2 macrophage polarization. Furthermore, we demonstrated that Bufalin targeted the SRC-3 protein to reduce MIF release in chemoresistant cells in order to regulate the polarization of M2 macrophages. More interestingly, we also found that Cinobufacini, Bufalin is its main active monomer, which its could regulate the polarization of M2 macrophages to enhance the anti-tumour effect of oxaliplatin in vivo and in the clinic. Overall, this study provides a theoretical basis for the clinical application of drugs containing Bufalin as the main active ingredient in combination with established chemotherapy for the treatment of colorectal cancer.

Growing evidence indicates that prorenin receptor (PRR) is upregulated in collecting duct (CD) of diabetic kidney. Prorenin is secreted by the principal CD cells, and is the natural ligand of the PRR. PRR activation stimulates fibrotic factors, including fibronectin, collagen, and transforming growth factor-β (TGF-β) contributing to tubular fibrosis. However, whether high glucose (HG) contributes to this effect is unknown. We tested the hypothesis that HG increases the abundance of PRR at the plasma membrane of the CD cells, thus contributing to the stimulation of downstream fibrotic factors, including TGF-β, collagen I, and fibronectin. We used streptozotocin (STZ) male Sprague–Dawley rats to induce hyperglycemia for 7 days. At the end of the study, STZ-induced rats showed increased prorenin, renin, and angiotensin (Ang) II in the renal inner medulla and urine, along with augmented downstream fibrotic factors TGF-β, collagen I, and fibronectin. STZ rats showed upregulation of PRR in the renal medulla and preferential distribution of PRR on the apical aspect of the CD cells. Cultured CD M-1 cells treated with HG (25 mM for 1 h) showed increased PRR in plasma membrane fractions compared to cells treated with normal glucose (5 mM). Increased apical PRR was accompanied by upregulation of TGF-β, collagen I, and fibronectin, while PRR knockdown prevented these effects. Fluorescence resonance energy transfer experiments in M-1 cells demonstrated augmented prorenin activity during HG conditions. The data indicate HG stimulates profibrotic factors by inducing PRR translocation to the plasma membrane in CD cells, which in perspective, might be a novel mechanism underlying the development of tubulointerstitial fibrosis in diabetes mellitus.

The polymers with pH responsiveness and temperature sensitivity exhibit important applications in many fields. To endow the responsive polymers with self-healing is meaningful work, which contributes to increase their service life and reduce waste of resources significantly. In this research, a series of pH-responsive polyurethanes containing dynamic disulfide bonds and carboxylic acid functional groups were prepared by mixing polycaprolactone diol (PCL), hexamethylene diisocyanate (HDI), 2,2-dimethylolbutyric acid, and bis(2-hydroxyethyl) disulfide. The structure of the polymer was confirmed by some characterization methods such as infrared absorption spectroscopy, Raman scattering spectroscopy, X-ray diffraction, and differential scanning calorimetry. Many performances of the polymer such as the contact angle, thermal stability, mechanics, and self-healing properties can be adjusted by changing the functional units of polyurethanes. The dynamic disulfide bonds in the main chain were observed no harm to the pH response performance, instead which were beneficial to the promotion of heat resistance, tensile properties, and self-healing performance of polyurethane. The elongation at break and the tensile strength are increased by 85.3% and 54.9%, respectively. All the polyurethane exhibited considerable self-healing effects at 110°C, with the highest healing efficiency reaching 93.7%, as a result of the dissociation of hydrogen bonds and the exchange reaction of disulfide bonds.

Glaesserella parasuis causes porcine Glasser's disease and lipopolysaccharide (LPS) induces acute inflammation and pathological damage. Baicalin has antioxidant, antimicrobial, and anti-inflammatory functions. Long noncoding RNAs (lncRNAs) play key regulatory functions during bacterial infection. However, the role of lncRNAs in the vascular dysfunction induced by a combination of G. parasuis and LPS during systemic inflammation and the effect of baicalin on lncRNA expression induced in porcine aortic vascular endothelial cells (PAVECs) by a combination of G. parasuis and LPS have not been investigated. In this study, we investigated the changes in lncRNA and mRNA expression induced in PAVECs by G. parasuis, LPS, or a combination of G. parasuis and LPS, and the action of baicalin on lncRNA expression induced in PAVECs by the combination of G. parasuis and LPS. Our results showed 133 lncRNAs and 602 genes were differentially expressed when PAVECs were stimulated with the combination of G. parasuis and LPS, whereas 107 lncRNAs and 936 genes were differentially expressed when PAVECs were stimulated with the combination of G. parasuis and LPS after pretreatment with baicalin. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed the dominant signaling pathways triggered by the combination of G. parasuis and LPS were the tumor necrosis factor signaling pathway, phosphatidylinositol signaling system, and inositol phosphate metabolism. Protein-protein interaction network analysis showed the differentially expressed target genes of the differentially expressed lncRNAs (DELs) were related to each other. A coexpression analysis indicated the expression levels of the DELs were co-regulated with those of their differentially expressed target genes. This is the first study to systematically compare the changes in lncRNAs and mRNAs in PAVECs stimulated with a combination of G. parasuis and LPS. Our data clarified the mechanisms underlying the vascular inflammation and damage triggered by G. parasuis and LPS, and it may provide novel targets for the treatment of LPS-induced systemic inflammation.

Growing evidence indicates that N6-methyladenosine (m6A) is the most pervasive RNA modification in eukaryotic cells. However, the specific role of METTL3 in papillary thyroid carcinoma (PTC) initiation and development remains elusive. Here we found that downregulation of METTL3 was correlated with malignant progression and poor prognosis in PTC. A variety of gain- and loss-of-function studies clarified the effect of METTL3 on regulation of growth and metastasis of PTC cells in vitro and in vivo. By combining RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation sequencing (meRIP-seq), our mechanistic studies pinpointed c-Rel and RelA as downstream m6A targets of METTL3. Disruption of METTL3 elicited secretion of interleukin-8 (IL-8), and elevated concentrations of IL-8 promoted recruitment of tumor-associated neutrophils (TANs) in chemotaxis assays and mouse models. Administration of the IL-8 antagonist SB225002 substantially retarded tumor growth and abolished TAN accumulation in immunodeficient mice. Our findings revealed that METTL3 played a pivotal tumor-suppressor role in PTC carcinogenesis through c-Rel and RelA inactivation of the nuclear factor κB (NF-κB) pathway by cooperating with YTHDF2 and altered TAN infiltration to regulate tumor growth, which extends our understanding of the relationship between m6A modification and plasticity of the tumor microenvironment.

OBJECTIVE: This study aimed to evaluate the effect of adiposity and fat distribution on the odds of elevated cardiovascular risk factors among adults with type 2 diabetes mellitus. METHODS: The present cross-sectional study included 2,427 adults with type 2 diabetes mellitus. Body fat was assessed by dual-energy x-ray absorptiometry. Multivariate-adjusted logistic regression was used to estimate effects of adiposity parameters on elevated hemoglobin A(1c) (HbA(1c), ≥7.0%), hypertension (blood pressure ≥140/90 mmHg), and elevated low-density lipoprotein (LDL) cholesterol (≥2.6 mmol/L). RESULTS: The multivariable-adjusted odds ratio (OR) for elevated HbA(1c) was 0.82 (95% CI: 0.70–0.96) for each SD increase in leg fat mass. The multivariable-adjusted OR for hypertension was 1.15 (95% CI: 1.00–1.32) for each SD increase in android fat mass. Multivariable-adjusted ORs for elevated LDL cholesterol ranged from 1.16 (95% CI: 1.00–1.35) to 1.27 (95% CI: 1.06–1.51) for each SD increase in arm and android fat mass and percentage of total, truncal, arm, and android fat. Each SD increase in BMI, truncal-to-leg fat ratio, and android-to-gynoid fat ratio was significantly associated with increased risks of elevated HbA(1c), hypertension, and elevated LDL cholesterol. CONCLUSIONS: Subcutaneous fat in the lower body was associated with a more favorable glycemic profile, but not blood pressure or lipid profile, whereas central adiposity was associated with poor control of cardiovascular risk factors among patients with type 2 diabetes mellitus.

Methylophiopogonanone A (MOA), an abundant homoisoflavonoid bearing a methylenedioxyphenyl moiety, is one of the major constituents in the Chinese herb Ophiopogon japonicas. This work aims to assess the inhibitory potentials of MOA against cytochrome P450 enzymes and to decipher the molecular mechanisms for P450 inhibition by MOA. The results showed that MOA concentration-dependently inhibited CYP1A, 2C8, 2C9, 2C19, and 3A in human liver microsomes (HLMs) in a reversible way, with IC50 values varying from 1.06 to 3.43 μM. By contrast, MOA time-, concentration-, and NADPH-dependently inhibited CYP2D6 and CYP2E1, along with KI and kinact values of 207 µM and 0.07 minute−1 for CYP2D6, as well as 20.9 µM and 0.03 minutes−1 for CYP2E1. Further investigations demonstrated that a quinone metabolite of MOA could be trapped by glutathione in an HLM incubation system, and CYP2D6, 1A2, and 2E1 were the major contributors to catalyze the metabolic activation of MOA to the corresponding O-quinone intermediate. Additionally, the potential risks of herb-drug interactions triggered by MOA or MOA-related products were also predicted. Collectively, our findings verify that MOA is a reversible inhibitor of CYP1A, 2C8, 2C9, 2C19, and 3A but acts as an inactivator of CYP2D6 and CYP2E1. Significance Statement Methylophiopogonanone A (MOA), an abundant homoisoflavonoid isolated from the Chinese herb Ophiopogon japonicas, is a reversible inhibitor of CYP1A, 2C8, 2C9, 2C19, and 3A but acts as an inactivator of CYP2D6 and CYP2E1. Further investigations demonstrated that a quinone metabolite of MOA could be trapped by glutathione in a human liver microsome incubation system, and CYP2D6, 1A2, and 2E1 were the major contributors to catalyze the metabolic activation of MOA to the corresponding O-quinone intermediate.