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Ultrathin, ultrastrong, and highly conductive solid-state polymer-based composite electrolytes have long been exploited for the next-generation lithium-based batteries. In particular, the lightweight membranes that are less than tens of microns are strongly desired, aiming to maximize the energy densities of solid-state batteries. However, building such ideal membranes are challenging when using traditional materials and fabrication technologies. Here we reported a 7.1 μm thick heterolayered Kevlar/covalent organic framework (COF) composite membrane fabricated via a bottom-up spin layer-by-layer assembly technology that allows for precise control over the structure and thickness of the obtained membrane. Much stronger chemical/mechanical interactions between cross-linked Kevlar and conductive 2D-COF building blocks were designed, resulting in a highly strong and Li+ conductive (1.62 × 10-4 S cm-1 at 30 °C and 4.6 × 10-4 S cm-1 at 70 °C) electrolyte membrane that can prevent solid-state batteries from short-circuiting after over 500 h of cycling. All-solid-state lithium batteries using this membrane enable a significantly improved energy density.

In iron-containing silicate melts such as gasification slags and geological slags, iron can play a different role in the melt properties because of its varying valence states. In this paper, the st...

Petroleum coke (petcoke) ash fusibility is closely related to the ash-related fouling and slagging, which have significant effects on its clean and efficient utilization. Sodium (Na) element in pet...

Experimental measurements of the laminar flame speed and extinction strain rate of premixed methanol/air flames were conducted using the counterflow configuration. The validities of five methanol o...

Increasing evidence supports the essential roles of circular RNAs (circRNAs) and microRNAs (miRNAs/miRs) in different types of human cancer. For example, hsa_circ_0137008 functions as a sponge for mi-338-5p and inhibits the malignant phenotype in colorectal cancer. Furthermore, hsa_circ_RNA_0011780 downregulates FBXW7 by targeting miR-554a and suppressing the progression of non-small cell lung cancer. Thus far, only a single report has identified that the miRNA miR-331-3p exerts a pivotal effect on human colorectal cancer (CRC) evolution. However, both the up- and downstream regulatory mechanisms of miR-331-3p are unclear. In the present study, it was predicted via bioinformatics analysis that the circRNA, hsa_circ_0038646, and the glutamate receptor ionotropic kainate 3 (GRIK3) gene contain binding sites that can interact with miR-331-3p. Thus, hsa_circ_0038646/miR-331-3p/GRIK3 may be a novel therapeutic pathway for CRC. Reverse transcription-quantitative PCR and western blotting analyses were performed, as well as cell proliferation, luciferase reporter and Transwell migration assays. Hsa_circ_0038646 was overexpressed in both CRC cells and tissues, and this aberrant expression was positively related with increasing tumor grade. Knockdown of hsa_circ_0038646 significantly weakened human CRC cell proliferation and migration. It was shown that hsa_circ_0038646 can sponge miR-331-3p to suppress its expression, and that suppression of miR-331-3p can reverse the effects of hsa_circ_0038646 inhibition in CRC cells. It was determined that GRIK3 is a downstream target of miR-331-3p, and that hsa_circ_0038646 could increase the levels of GRIK3 by suppressing miR-331-3p in CRC cells. Restoring GRIK3 expression rescued the weakened CRC cell proliferation and migration following hsa_circ_0038646 knockdown. The present study indicated that hsa_circ_0038646 functions as a tumor promoter in CRC by increasing GRIK3 expression via sponging of miR-331-3p. The hsa_circ_0038646/miR-331-3p/GRIK3 axis may be a novel therapeutic and diagnostic target of CRC.

In this paper, experimental and numerical studies on the decomposition pyrene, a main component of tar, under the steam reforming condition were conducted. The effects of operational conditions of reaction temperature TR and steam to carbon molar ratio (S/C) on the yield of main light gases and soot are assessed. The associated global reaction rate and main reaction pathways were analyzed. The results revealed that pyrene was mostly converted into soot instead of light gases in the steam reforming. The yields of CO2 and H2 significantly increased with TR, whereas those of CO and CH4 remained relatively stable. The water shift reaction of exhaust gases at low temperature zone should be taken into account to accurately predicted the yield of light gases. The conversion of pyrene rapidly happened when S/C was

In this paper, the elimination of heavy tar by steam reforming at high temperature was studied with experiments and numerical simulation. The experiments were conducted in a tube reactor with five model compounds at temperatures of 1273–1673 K, steam of 0–40 vol% and sample weight of 3–20 mg with residence time of 2 s. The simulation was performed with the plug flow model in CHEMKIN program based on a kinetic model that consists of >200 chemical species and 2000 elementary step-like reactions. The results of experiments indicate that increasing temperature will accelerate the decomposition of heavy tar; moisture could significantly prompt the decomposition of heavy tars, but not so efficient above 20 vol%; less sample could help the decomposition of heavy tar; longer residence time can slightly increase the decomposition of heavy tar. At high temperature (1573 K or above), the heavy tar is mainly cracked into two parts: the one carbon molecules (CO and CO 2 ) and the soot at the beginning of reactor. Then the soot decomposes into CO, CO 2 and H 2 by steam reforming. The conversion and kinetic data of heavy tar can be divided by two temperature sections. The carbon conversion degrees of different model compounds from 1173 K to1473 K are close with similar trends, which may be represented by a universal kinetic model. Our results provide an insight in the understanding of the heavy tar decomposition and give the necessary information for the designation and operation of the tar cracker to eliminate heavy tar.

Hydrogen-enriched natural gas is a promising low-carbon fuel in combustion devices. To better assess its feasibility from the extinction prevention point of view, the extinction of near-limit premixed hydrogen-methane/air flames over a wide range of equivalence ratios was measured using the single-flame counterflow configuration. Results showed that the hydrogen addition resulted in a greater extinction stretch rate. Further analysis demonstrated that the extinction stretch rate of premixed hydrogen-methane/air flames linearly correlated with the corresponding reference flame speed. Thereby a combustion regime map diagram, separating the burning and extinction, was sketched. In addition, critical extinction Damkӧhlor number of premixed hydrogen-methane/air flames was investigated and it was found to be insensitive to the hydrogen addition and equivalence ratio. Finally, the hydrogen addition effect and the corresponding extinction response were assessed for two scenarios, 1) constant thermal load (gas turbine, gas-fired boilers, etc.), and 2) constant fuel injection pressure (gas stove, oven, etc.). The results indicated that H2 addition promoted the combustion safety by increasing the extinction stretch rates, but an allowable H2 variation window existed when considering the unexpected high-temperature damage of the burners. The allowable H2 variation window of Scenario 1 was found much broader than that of Scenario 2.

Crystallization inside the liquid slag of entrained flow gasifiers can increase the viscosity of the slag, affecting flow down the wall and resulting in operational problems. The chemical component of the slag is an intrinsic factor of crystallization characteristics. In the study presented here, Differential Scanning Calorimetry (DSC) and the Single Hot Thermocouple Technique (SHTT) were combined to investigate the influence of (SiO 2 + Al 2 O 3 ) ratios on crystallization temperature and crystalline morphology, and to construct temperature-time transformation (TTT) diagrams. Five synthetic slags with different amounts of silica and alumina (silica/alumina keeps constant) were prepared for their crystallization tendency. The mass ratio of (SiO 2 + Al 2 O 3 ) ranges from 30 to 70 wt.%. For the kinetics calculation, the Kissinger method was applied for activation energy under cooling conditions with DSC results and the JMA equation was used for crystallization mechanisms based on SHTT results under isothermal conditions. Bulk quenching samples were produced in a high temperature quenching furnace and analyzed by XRD to determine phases. The thermodynamic modeling program FactSage was also applied to predict the equilibrium composition and compared with experimental results. It turns out that crystallization of the slag with high amount of (SiO 2 + Al 2 O 3 ) was so weak that the crystals could hardly be observed. Higher basic oxides favored crystallization and led to higher crystallization temperature. When the proportion of (SiO 2 + Al 2 O 3 ) reduced to 30%, the crystallization tendency was extremely strong that the crystals formed at 1380 °C.

A comprehensive three-dimensional numerical model has been developed to for entrained flow coal hydrogasifier reactor. The kinetic model includes water evaporation, Johnson and Tran hydropyrolysis model, semichar reactions and secondary tar hydrocracking reactions. Numerical CFD(Computational Fluid Dynamics) simulation were performed using this model on a 6 t/d hydrogasifier reactor and the predictions are in good agreement with experimental results on coal conversion on a Dry Ash-Free (DAF) basis, overall carbon conversion, carbon conversions to the light hydrocarbon gases (LHGs, mainly methane) and the temperature at the outlet of the reactor. The performance of the reactor with different structural parameters (length-diameter draw ratio H / D , collision angle α , impinging distance h ) was compared, including the flow field, temperature distribution and carbon conversion to various products. The results show that larger H/D , smaller collision angle and impinging distance are favorable for higher carbon conversion and CH 4 production. But the influence of structural parameters on operating performance is not obvious.

Succinate dehydrogenase is a heterotetrameric complex comprising four nuclear-encoded subunits, catalyzes the oxidation of succinate to fumarate in the tricarboxylic acid cycle. A subset of cancers have been found to be associated with mutations in the four SDH genes. However, the functional roles of the SDH complex in tumorigenesis remain largely unclear, especially in hepatocellular carcinoma (HCC). Here, we investigated the expression levels of the four SDH subunits and their clinical significance in HCC, followed by systematic exploration of the effects of SDH dysfunction on HCC cell survival and metastasis both in vitro and in vivo, as well as the underlying molecular mechanisms. Our results showed that the expression of the SDHA/B/C/D subunits was significantly downregulated in HCC, associated with poor patient prognosis, and contributed to SDH inactivation. Additionally, attenuated SDH activity following SDHC knockdown promoted HCC-cell growth and metastasis both in vitro and in vivo via elevated reactive oxygen species levels and subsequent activation of nuclear factor-κB signaling. These findings suggest a critical tumor-suppressive role for SDH and provide strong evidence supporting this enzyme as a potential drug target in the treatment of HCC.

A kinetic model of hydropyrolysis was developed based on the Chemical Percolation Devolatilization (CPD) network devolatilization model, considering the hydrogenation reactions of active species and fragments produced during the decomposition of the coal structure with hydrogen. Light gas compositions were calculated and the yields of oxygen-containing species were corrected. The calculation results show that the model well predicts the yields of volatile matter and methane for the coals that are in the scope of application of the correlation of the chemical structure parameters, with the value of β ranging from 0.0035 to 0.004 atm −1 , where β is a coefficient related to the ratio of the reaction rate of hydrogenation to that of crosslinking. The model successfully predicts the influence of coal type, final temperature and pressure on the hydropyrolysis yield and gas compositions. Further studies are needed to validate the model and determine the value and influencing factors of β .

Sulfurized carbon is a promising candidate for cathode materials in practical lithium–sulfur batteries due to its high and stable capacity retention, extremely low self-discharge, and excellent safety. The main disadvantage is the relatively low sulfur content in sulfurized carbon materials. Borrowing the idea from rubber crosslinking chemistry, here we report the use of a vulcanization accelerator in carbon sulfurization. Vulcanization accelerators significantly improve sulfur content by ∼8 wt%, which results in a remarkable increase in the discharge capacity of corresponding Li–S batteries by ∼120 mA h g−1 while maintaining the outstanding cycling stability and low self-discharge. The effectiveness of this approach and the wide variety of vulcanization accelerator chemistry will pave the way for sulfurized carbon materials towards practical applications in Li–S batteries.

Aspirin is widely used in the treatment of a number of clinical conditions. Although aspirin is being thought to be a relatively "safe" medicine, it also has some side effects, particularly the risk of bleeding which may be severe and lead to death. The mechanisms, however, are not totally understood. It has been reported recently that aspirin induces apoptosis in many cell types. Thus, the aim of the current study is to explore whether aspirin induces platelet apoptosis. The data show that mitochondrial transmembrane potential (ΔΨm) depolarizations and phosphatidylserine (PS) exposures were dose-dependently induced by aspirin in platelets. To further confirm that aspirin incurs platelet apoptosis, caspase-3 activity was measured in platelets, and the result indicated that aspirin induced caspase-3 activation. Furthermore, the mean volume of platelets incubated with aspirin was obviously reduced. Caspase inhibitor z-VAD-fmk inhibited aspirin induced apoptotic platelet shrinkage and ΔΨm depolarization, but had no effect on PS exposure. In addition, platelets incubated with cyclooxygenase inhibitor indomethacin did not incur ΔΨm depolarazation and PS exposure. Taken together, the data indicate that aspirin induces platelet apoptosis via caspase-3 activation.

Ternary oxide Zn 2 SnO 4 was successfully introduced to SnO 2 hierarchical microsphere photoanode for dye-sensitized solar cells by spin-coating method. Zn 2 SnO 4 nanoparticles with average size of 20 nm modified the SnO 2 hierarchical microsphere film uniformly and sufficiently. The shell of Zn 2 SnO 4 nanoparticles plays an important role in resisting acid etchant and suppressing the recombination of conduction band electrons with the oxidized dye. Benefiting from the advantages of both constituents, the composite photoanode exhibits an improved chemical stability and good photoelectric properties in contrast with bare SnO 2 nanostructures.

Thin films of p-type Bi0.52Sb1.48Te3 + 3% Te were deposited on glass substrates by flash evaporation. X-ray diffraction and field-emission scanning electron microscopy were performed to characterize the thin films, and the effects of preparation and annealing parameters on the thermoelectric properties were investigated. It was shown that the power factors of the films increased with increasing deposition temperature. Annealing the as-deposited films improved the power factors when the annealing time was less than 90 min and the annealing temperature was lower than 250°C. A maximum power factor of 10.66 μW cm −1 K −2 was obtained when the film was deposited at 200°C and annealed at 250°C for 60 min. flash evaporation, thin film, thermoelectric properties, bismuth telluride

A double-layer (DL) film with a TiO2 nanosheet-layer on a layer of TiO2 nanorod-array, was synthesized on a transparent conductive fluorine-doped tin oxide substrate by a two-step hydrothermal method. Starting from the precursors of NaSeSO3, CdSO4 and the complex of N(CH2COOK)3, CdSe quantum dots (QDs) were grown on the DL-TiO2 substrate by chemical bath deposition method. The samples were characterized by X-ray diffraction, Scanning electron microscopy, Energy dispersion spectroscopy, and their optical scattering property was measured by light reflection spectrometry. Some CdSe QDs sensitized DL-TiO2 films serve as the photoanodes, were assembled into solar cell devices and their photovoltaic performance were also characterized. The short circuit current and open-circuit voltage of the solar cells range from 0.75 to 4.05 mA/cm2 and 0.20 − 0.42 V under the illumination of one sun (AM1.5, 100 mW/cm2), respectively. The photocurrent density of the DL-TiO2 film is five times higher than that of a bare TiO2 nanorod array photoelectrode cell.

Circulating adhesion molecules (CAMs), surface proteins expressed in the vascular endothelium, have emerged as risk factors for cardiovascular disease (CVD). CAMs are involved in intercellular communication that are believed to play a role in atherosclerosis. A Chinese medicine, the “Dantonic Pill” (DP) (also known as the “Cardiotonic Pill”), containing three Chinese herbal material medica, Radix Salviae Miltiorrhizae, Radix Notoginseng and Borneolum Syntheticum, has been used in China for the prevention and management of CVD. Previous laboratory and animal studies have suggested that this preparation reduces both atherogenesis and adhesion molecule expression. A parallel double blind randomized placebo-controlled study was conducted to assess the effects of the DP on three species of CAM (intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 and endothelial cell selectin (E-selectin)) in participants with mild-moderate hypercholesterolemia. Secondary endpoints included biochemical and hematological variables and clinical effects. Forty participants were randomized to either treatment or control for 12 weeks. Treatment with DP was associated with a statistically significant decrease in ICAM-1 (9% decrease,P= .03) and E-Selectin (15% decrease,P= .004). There was no significant change in renal function tests, liver function tests, glucose, lipids or C-reactive protein levels and clinical adverse effects did not differ between the active and the control groups. There were no relevant changes in participants receiving placebo. These results suggest that this herbal medicine may contribute to the development of a novel approach to cardiovascular risk reduction.

Char―CO 2 gasification reactions in the presence of CO and char-steam gasification reactions in the presence of H 2 were studied at the atmospheric condition using a thermogravimetric apparatus (TGA) at various reactant partial pressures and within a temperature range of 1123 K-1223 K. The char was prepared from a lignite coal. The partial pressure of H 2 and CO varied from 0.05 to 0.3 atm. The experimental results showed that Langmuir-Hinshelwood (L-H) kinetic equation was applicable to describe the inhibition effects of CO and H 2 . The kinetic parameters in L-H equations were obtained. Interactions of char gasification by steam and CO 2 in the presence of H 2 and CO were discussed. It was found that the kinetic parameters determined from pure or binary gas mixtures can be used to predict multi-component gasification rates. The results confirmed that the char-steam and char―CO 2 reactions proceed on separate active sites rather than common active sites.

A comprehensive three-dimensional (3D) numerical model is developed for simulation of entrained coal slurry gasifiers. In this model, a presumed probability density function (PDF) method is used to consider turbulent effects on gas-phase reactions in the gasifier. A realizable k−e model is adopted to predict turbulence information. Coal slurry particles are tracked with the Lagrangian method. The coal slurry gasification process is divided into several simple subprocesses, which are droplet evaporation, boiling, devolatilization, and heterogeneous reactions of coal char particles. The particle-source-in-cell method is adopted to couple gas−particle interactions. With this methodology, simulations for GE and staged coal slurry gasifiers are completed. A comparison between the predictions and measured data shows that the proposed model correctly predicts the global performance of the coal gasification process. The mixing process in the staged gasifier is better than that in the GE gasifier because of the ex...

There is a large range of time and length scales of turbulent fluctuations in an entrained flow coal gasifier. To figure out the turbulent effects on coal gasification processes and the controlling mechanisms of different regions in an entrained flow coal gasifier, the characteristic time and length scales for all major processes are estimated. On the basis of the comparison between the length/time scales of turbulent mixing in all ranges and the characteristic time of homo- and heterogeneous reactions, turbulent effects on reactions and coal gasification processes in different regions in an entrained coal gasifier are studied. In the flame region, there is a strong coupling effect between macro-scale turbulent fluctuation and heterogeneous reactions. The combustion of the volatile is strongly affected by the fluctuation of micro-scale Kolmogorov scales because the diffusion boundary layer of the particles is destroyed by this kind of fluctuation. In the nonflame region, the heterogeneous char gasificatio...

Bismuth selenide thin films were grown on Pt substrate via the route of electrochemical atomic layer epitaxy (ECALE) in this work for the first time. The electrochemical behaviors of Bi and Se on bare Pt, Se on Bi-covered Pt, and Bi on Se-covered Pt were studied by cyclic voltammetry and coulometry. A steady deposition of Bi 2 Se 3 could be attained after negatively stepped adjusting of underpotential deposition (UPD) potentials of Bi and Se on Pt in the first 40 deposition cycles. X-ray diffraction (XRD) analysis indicated that the films were single phase Bi 2 Se 3 compound with orthorhombic structure, and the 2:3 stoichiometric ratio of Bi to Se was verified by EDX quantitative analysis. The optical band gap of the as-deposited Bi 2 Se 3 film was determined as 0.35 eV by Fourier transform infrared spectroscopy (FTIR), which is consistent with that of bulk Bi 2 Se 3 compound.

Hydrogen formed in oxidative coupling of methane (OCM) over BaCO3 and MgO catalysts was measured since the data of H2 selectivity were missing almost in all articles published heretofore. It was found that H2 selectivity achieved about 18%, when C2 hydrocarbon's selectivity was maintained at 48%–45% over BaCO3 catalyst at the feed molar ratio of CH4/O2 = 4 in temperature range of 780 °C–820 °C. Under similar conditions, H2 selectivity was about 14%–16% over MgO catalyst, with C2 selectivity maintained at 41%–42%. Possible routes for hydrogen formation in OCM reaction were discussed. Effect of addition of alkali metallic ions was also investigated.

Measurement of temperature profiles of gas-solid two-phase flow at different heights in commercial-scale circulating fluidized bed (CFB) boilers was carried out. Experimental results showed that the thickness of thermal boundary layer was generally independent of the distance from the air distributor, except when close to the riser outlet. Through analysis of flow and combustion characteristics in the riser, it was found that the main reasons for the phenomena were: 1) the hydrodynamic boundary layer was thinner than the thermal layer and hardly changed along the CFB boiler height, and 2) both radial and axial mass and heat exchanges were strong in the CFB boiler. Numerical simulation of gas flow in the outlet zone confirmed that the distribution of the thermal boundary layer was dominated by the flow field characteristics.

BACKGROUND: Staurosporine (STS), a microbial alkaloid and potent PKC inhibitor, has become one of the most promising anti-cancer drugs. STS effectively induces apoptosis in many nucleated cells; however, it is still unclear whether STS induces apoptosis in enucleated platelets. METHODS: Apoptotic events in platelets treated with STS were assessed by flow cytometry or western blotting. RESULTS: STS induced depolarization of mitochondrial inner transmembrane potential (ΔΨm), up-regulation of Bax and Bak, phosphatidylserine (PS) exposure, release of mitochondrial cytochrome c, and activation of caspase-8 and caspase-9 in human platelets. Furthermore, STS stimulation induced phosphorylation of p38 mitogen-activated protein kinase (MAPK). Inhibition of p38 MAPK activation significantly reduced ΔΨm depolarization and PS exposure in platelets stimulated with STS. CONCLUSIONS: These data indicate that STS induces platelet apoptosis via the p38 MAPK signaling pathway. These findings suggest that platelet apoptosis-related hemorrhage should be noticed in STS and its derivatives in clinical tests.

The effects of alloying elements on the macrostructures, microstructures and tensile strength of AZ80 Mg alloy weldments were studied in the present study. The results indicate that with the decrease of Al element content of filler wire, the welding defects of seam are gradually eliminated and the β-Mg17Al12 phases at α-Mg boundaries are refined and become discontinuous, which are beneficial to the improvement of tensile strength. With AZ31 Mg alloy filler wire, the maximum tensile strength of AZ80 weldment is 220 MPa and fracture occurs at the welding seam of joint. It is experimentally proved that robust AZ80 Mg alloy joints can be obtained by tungsten inert gas (TIG) welding process with AZ31 Mg alloy filler wire. However, further study is required to improve the microstructures and reduce welding defects of joint in order to further improve the joining strength of AZ80 Mg alloy joint.

AgSn18SbTe20 and Sn50Te50 thermoelectric materials are prepared by mechanical alloying from powder mixtures of the elements followed by plasma-activated sintering (653 K, 15 min).

CFB fly ash from separators was mixed with water or the mixture of water and additives under the temperature of 363K by use of a blender. Then, this compound of fly ash and water or additives was pumped into a CFB combustion chamber by a sludge pump. Because the temperature of flue gas was high in CFB, the fly ash was hydrated fast and agglomerated in the same time. Through this process, the size of agglomerating fly ash is larger than the original particle and the relative residence time of agglomerated fly ash in CFB becomes longer. Therefore, the rate of utility of calcium in fly ash improves and the content of carbon in fly ash decreases. This results in a low Ca/S and low operational cost for CFB boiler. The additive is one key factor, which affects the rate of desulfurization of agglomerated fly ash. Effect of different additives on rate of desulfurization is not same. Cement and limestone are beneficiated to sulfur removal of agglomerated fly ash, but sodium silicate does not devote to the rate of sulfur removal of agglomerated fly ash.

In this paper, a self-assembling double-layered film consisting of SnO2 nanosheet arrays as an underlayer and hierarchical SnO2 microspheres as an overlayer was fabricated via a facile hydrothermal process. The effect of experimental parameters, such as seed layer, acetylacetone, and NH4F on the morphology of the SnO2 hierarchical double-layered film was investigated. We disclose that these hierarchical structures are the consequence of the simultaneous processes of growing and recrystallization on the FTO seeded surface and in solution. A formation mechanism was proposed to understand the growth process. The reflectance spectra of SnO2 double-layered films were also examined.The novel SnO2-based film shows an enhanced light-to-electricity conversion efficiency compared with a simple composite of SnO2 nanosheet arrays and microspheres due to their self-assembling capability and favorable nanostructures.

Abstract 5144 Aspirin is widely used in the treatment of a number of clinical conditions, such as fever, pain, strokes, and heart attacks. Although aspirin is being thought of a relatively “safety” medicine, it also has some side effects, particularly the risk of bleeding which may be severe and lead to death. The mechanisms, however, are not totally understood. The effectiveness of aspirin has been attributed to its ability to inhibit prostaglandin production by inhibiting the cyclooxygenase (COX) enzyme. Interestingly, it has been reported recently that aspirin induces apoptosis in many cell types. Platelet apoptosis induced by either physical or chemical compounds or platelet storage occurs widely. We have reported that the glycoprotein Ibα-von Willebrand factor interaction, hyperthermia, and calmodulin antagonists induce platelet apoptosis, suggesting that platelet apoptosis might play important roles in controlling the number and function of circulated platelet under physiological conditions, or in the development of platelet-related hemorrhagic diseases. Thus, the aim of the current study is to explore whether aspirin induces platelet apoptosis. Washed platelets (3×108/ml) were incubated with various concentrations of aspirin (2. 5 mM, 5 mM, 10 mM, 20 mM) at 37 °C for 2 hours. The effect of aspirin on platelet mitochondrial membrane potential (ΔΨm) depolarization and phosphatidylserine (PS) exposure were analyzed by cell-permeable lipophilic cationic dye tetramethylrhodamine ethyl ester (TMRE) and annexin V binding, respectively. The data show that ΔΨms depolarization and PS exposures were dose-dependently induced by aspirin in platelets. In order to further confirm that aspirin incurs platelet apoptosis, caspase-3 activation was measured in platelets incubated with aspirin by detecting 32 kD pro-caspase-3 and 17 kD cleaved caspase-3 fragment, and the result shows that aspirin induced caspase-3 activation. Furthermore, platelet shrinkage appeared in platelets incubated with aspirin (20 mM). Caspase inhibitor z-VAD-fmk inhibited apoptotic platelet shrinkage, and blocked ΔΨm depolarization, but had no effect on PS exposure, suggesting that caspase-3 acts upstream of ΔΨm depolarization, and plays a key role in aspirin-induced platelet apoptosis. Whereas, protein kinase C (PKC), protein kinase B (PKB), p38 mitogen-activated protein kinases (MAPK), protein 53 (p53), reactive oxygen species (ROS), and mitogen-activated protein kinase kinase (MAPKK) were not involved in aspirin-induced platelet apoptosis. In addition, platelets incubated with another COX inhibitor indomethacin did not incur ΔΨm depolarazation and PS exposure in platelets. Taken together, the data indicate that aspirin induces platelet apoptosis via caspase-3 activation, and independent of the COX signaling. Disclosures: No relevant conflicts of interest to declare.